AirPods and Bluetooth Radiation: Safety Research

Age-dependent acute interference with stem and progenitor cell proliferation in the hippocampus after exposure to 1800 MHz electromagnetic radiation.

Xu F et al. · 2017

Ten gigahertz microwave radiation impairs spatial memory, enzymes activity, and histopathology of developing mice brain

Sharma A, Kesari KK, Saxena VK, Sisodia R. · 2017

Effects of prenatal exposure to WIFI signal (2.45GHz) on postnatal development and behavior in rat: Influence of maternal restraint.

Othman H, Ammari M, Sakly M, Abdelmelek H. · 2017

Researchers randomly assigned 24 pregnant albino Wistar rats to four groups: control, WiFi-exposed, restrained, and both WiFi-exposed and restrained. Each WiFi exposure and restraint session lasted 2 hours per day throughout gestation until parturition. Prenatal WiFi exposure and restraint, separately and especially combined, adversely affected offspring physical development, neuromotor maturation, adult emotional responses, motor and exploratory behavior, brain oxidative stress, and serum biochemistry, with most impacts depending on offspring gender.

Effects of short and long term electromagnetic fields exposure on the human hippocampus

Deniz OG et al. · 2017

Researchers studied 60 healthy female medical students divided into low (<30 min daily) and high (>90 min daily) mobile phone exposure groups. Hippocampal volume and metabolite levels did not differ significantly between groups. However, the high exposure group performed significantly worse on Stroop and digit span backward tests of attention.

Personal radiofrequency electromagnetic field exposure measurements in Swiss adolescents.

Roser K et al. · 2017

Researchers measured personal RF-EMF exposure in 90 adolescents aged 13 to 17 years using a portable ExpoM-RF device across 13 frequency bands from 470 to 3600 MHz for three consecutive days with a time-activity diary. Mobile phones contributed 67.2% and mobile phone base stations contributed 19.8% of total personal RF-EMF measurements averaging 63.2 μW/m² (0.15 V/m). WLAN at school and home accounted for 3.5% of total personal measurements. Environmental sources contributed on average 6.0% to the brain dose and 9.0% to the whole-body dose according to dose calculations.

Effects of GSM and UMTS mobile telephony signals on neuron degeneration and blood-brain barrier permeation in the rat brain.

Poulletier de Gannes F et al. · 2017

Oxidative stress response in SH-SY5Y cells exposed to short-term 1800 MHz radiofrequency radiation

Marjanovic Cermak AM, Pavicic I, Trosic I · 2017

Human neuroblastoma SH-SY5Y cells were exposed to 1800 MHz radiofrequency radiation for 10, 30, and 60 minutes at an electric field strength of 30 V m-1 and a specific absorption rate of 1.6 W kg-1. Cell viability remained within normal physiological values after radiation exposure. Significantly higher ROS levels were observed at every radiation exposure time tested. After 60 minutes of exposure, the radiation caused significant lipid and protein damage. The highest glutathione concentration was detected after 10 minutes of exposure.

Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex.

Lameth J et al. · 2017

Researchers investigated whether a 2-hour head-only exposure to GSM-1800 MHz could affect a neuroinflammatory reaction triggered by lipopolysaccharide in 2-week-old or adult rats. In developing rats, 24 hours after exposure, cortical interleukin-1β and NOX2 NADPH oxidase transcripts were reduced by 50 to 60% compared with sham-exposed animals. Adult rats showed a 50% reduction in IL1β mRNA but no NOX2 suppression and displayed a significant growth response of microglial cell processes. GSM exposure decreased phosphorylation at two residues on the GluA1 AMPA receptor subunit (serine 831 and 845). The changes in gene expression, microglia, and GluA1 phosphorylation did not persist 72 hours after exposure and were absent without LPS pretreatment.

Exposure to 835 MHz radiofrequency electromagnetic field induces autophagy in hippocampus but not in brain stem of mice.

Kim JH et al. · 2017

Researchers exposed C57BL/6 mice to 835 MHz RF-EMF at an SAR of 4.0 W/kg for 12 weeks, then analyzed the hippocampus and brain stem. Several autophagic genes were significantly upregulated only in the hippocampus and not in the brain stem. Transmission electron microscopy revealed an increase in the number of autophagosomes and autolysosomes in the hippocampal neurons of exposed mice.

Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field.

Kim JH, Kim HJ, Yu DH, Kweon HS, Huh YH, Kim HR. · 2017

Researchers studied the effects of RF-EMF exposure on synaptic vesicles in the cortical neurons of mice. C57 BL/6 mice were exposed to 835 MHz RF-EMF at 4.0 W/kg SAR for 5 hours daily. The density of synaptic vesicles was significantly decreased in the presynaptic boutons of cortical neurons after exposure. The expression of synapsins I/II genes and proteins was also significantly decreased in exposed mice.

Histopathological, immunohistochemical, and stereological analysis of the effect of Gingko biloba (Egb761) on the hippocampus of rats exposed to long-term cellphone radiation.

Gevrek F. · 2017

Rats were divided into three groups — Control, EMR, and EMR+Egb761 — and the EMR groups were exposed to cellphone radiation at 0.96 W/kg SAR for one month. In the EMR group, apoptotic proteins (Bax, Acas-3) increased and the anti-apoptotic protein Bcl-2 decreased, along with a reduction in total granule and pyramidal cell counts. Rats that also received Egb761 showed decreased Bax and Acas-3, increased Bcl-2, fewer TUNEL-stained apoptotic cells, and a higher total viable cell number.

Evaluation of the potential of mobile phone specific electromagnetic fields (UMTS) to produce micronuclei in human glioblastoma cell lines.

Al-Serori H et al. · 2017

Researchers investigated whether UMTS radiofrequency signals cause micronucleus formation and other anomalies such as nuclear buds and nucleoplasmatic bridges in human glioblastoma cell lines. The experiments were conducted with human glioblastoma cell lines, U87 with wild-type p53 status and U251 with mutated p53 status, exposed to SAR doses of 0.25, 0.50, and 1.00 W/kg. The researchers found no evidence for induction of micronuclei or other anomalies, but observed apoptosis at the highest dose in U251 cells.

Microwave radiation (2.45 GHz)-induced oxidative stress: Whole-body exposure effect on histopathology of Wistar rats.

Chauhan P, Verma HN, Sisodia R, Kesari KK. · 2017

Researchers exposed male Wistar rats to 2.45 GHz microwave radiation at a power density of 0.2 mW/cm2 for 2 hours a day for 35 days, then examined brain, liver, kidney, testis, and spleen. Significantly high levels of lipid peroxide were found in the liver (p < 0.001), brain (p < 0.004), and spleen (p < 0.006) of exposed rats. Histological changes were also observed in the brain, liver, testis, kidney, and spleen after whole-body microwave exposure, compared to the control group.

The antioxidant effect of Green Tea Mega EGCG against electromagnetic radiation-induced oxidative stress in the hippocampus and striatum of rats

Ahmed NA, Radwan NM, Aboul Ezz HS, Salama NA · 2017

Researchers exposed adult rats to EMR at a frequency of 900 MHz modulated at 217 Hz, with a power density of 0.02 mW/cm2 and SAR of 1.245 W/kg, and measured oxidative stress parameters in the hippocampus and striatum. EMR exposure produced disturbances in oxidant and antioxidant parameters in both brain regions. Green tea mega EGCG given at the beginning of EMR exposure for two and three months had more beneficial effect against EMR-induced oxidative stress than EGCG given after two months of exposure.

Effect of 1.8 GHz radiofrequency electromagnetic radiation on novel object associative recognition memory in mice.

Wang K et al. · 2017

Researchers examined the effects of a single 30-minute exposure to 1.8 GHz radiofrequency electromagnetic radiation on recognition memory in mice, using the novel object recognition task. Exposure at an intensity above 2.2 W/kg SAR produced a significant, density-dependent increase in the recognition memory index, with no corresponding changes in spontaneous locomotor activity. The researchers also found that exposure increased dendritic-spine density and length in hippocampal and prefrontal cortical neurons, and significantly altered neuronal excitability in pyramidal neurons. The SAR used in the study was 3.3 W/kg, which the researchers noted was outside the range encountered in normal daily life.

Long term impairment of cognitive functions and alterations of NMDAR subunits after continuous microwave exposure

Wang H et al. · 2017

Researchers exposed 220 male Wistar rats to a 2.856 GHz radiation source at power densities of 0, 2.5, 5, and 10 mW/cm² for 6 min/day, 5 days/week, for up to 6 weeks. Rats in the 10 mW/cm² group showed declines in spatial learning and memory and EEG disorders, including decreased EEG frequencies and increased amplitudes and delta wave powers. Changes in the basic structure and ultrastructure of the hippocampus were found in the 10 and 5 mW/cm² groups. The researchers suggested that continuous microwave exposure could cause dose-dependent long-term impairment of spatial learning and memory, and that decreases in NMDAR key subunits and phosphorylation of NR2B might contribute to cognitive impairment.

Long-term exposure to 835 MHz RF-EMF induces hyperactivity, autophagy and demyelination in the cortical neurons of mice

Kim JH, Yu DH, Huh YH, Lee EH, Kim HG, Kim HR. · 2017

Researchers exposed C57BL/6 mice to 835 MHz RF-EMF at a specific absorption rate of 4.0 W/kg for 5 hours per day during 12 weeks. The study found that RF-EMF exposure led to myelin sheath damage and that mice displayed hyperactivity-like behavior. Autophagy-related proteins including LC3B-II and Beclin1 were increased in neuronal cell bodies, and the researchers suggest autophagy may act as a protective pathway during radiofrequency exposure.

1950 MHz radiofrequency electromagnetic fields do not aggravate memory deficits in 5xFAD mice.

Son Y et al. · 2016

Researchers exposed 5xFAD mice to 1950 MHz RF-EMF at 5 W/kg SAR for 2 hours daily, 5 days a week, for 3 months. They used Y-maze, Morris water maze, and novel object recognition tests to assess spatial and non-spatial memory. They also measured Aβ deposition, APP and CTFβ levels in hippocampus and cortex, and plasma Aβ peptides. The 3-month exposure did not affect Aβ-related memory impairment or Aβ accumulation.

Cerebral radiofrequency exposures during adolescence: Impact on astrocytes and brain functions in healthy and pathologic rat models.

Petitdant N et al. · 2016

Researchers used rats to assess whether astrocyte reactivity, perception, and emotionality were affected by RF EMF exposures during adolescence. They also investigated if adolescent brains were more sensitive to RF EMF exposures after neurodevelopmental inflammation. Results did not show any neurobiological impairment in healthy and vulnerable RF EMF-exposed rats compared to sham-exposed controls. Data did not support the hypothesis of a specific cerebral sensitivity to RF EMF of adolescents, even after neurodevelopmental inflammation.

Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields.

McNamee JP et al. · 2016

Adult male C57BL/6 mice were exposed to 1.9 GHz pulse-modulated or continuous-wave radiofrequency fields for 4 hours per day over 5 days at specific absorption rates of 0, 0.2, and 1.4 W/kg. Total RNA was isolated from the auditory cortex, amygdala, caudate, cerebellum, hippocampus, hypothalamus, and medial prefrontal cortex and gene expression was analyzed using Illumina MouseWG-6 (v2) BeadChip arrays. When controlling the false discovery rate, no differentially expressed genes were identified in any brain region compared to sham controls. Some genes showed differences without multiple-comparison correction, but no consistent pattern emerged across exposure levels or modulations.

Mobile phone use, behavioural problems and concentration capacity in adolescents: A prospective study

Roser K, Schoeni A, Röösli M · 2016

The HERMES study sample consisted of 439 Swiss adolescents aged 12–17 years. Researchers assessed behavioral problems with the Strengths and Difficulties Questionnaire and concentration capacity with a standardized computerized cognitive test named FAKT. Cross-sectional analyses showed behavioral problems associated with self-reported wireless device use but not operator-recorded mobile phone use, while concentration capacity was associated with both self-reported and operator-recorded exposures. Longitudinal analyses found no consistent associations between RF-EMF exposure measures and either outcome. The authors suggest information bias and reverse causality likely explain the cross-sectional findings.

Effects on auditory function of chronic exposure to electromagnetic fields from mobile phones.

Bhagat S, Varshney S, Bist SS, Goel D, Mishra S, Jha VK · 2016

Each participant's phone-using ear was compared to the non-phone-using ear, and subjects were grouped by daily use duration of 60 minutes or less versus more than 60 minutes. No statistically significant differences were found in high-frequency pure-tone averages or brainstem evoked response audiometry interpeak latencies between phone-using and control ears, and no subjective symptoms were reported.

Social behavioral testing and brain magnetic resonance imaging in chicks exposed to mobile phone radiation during development.

Zhou Z, Shan J, Zu J, Chen Z, Ma W, Li L, Xu J. · 2016

At day 4 of incubation, 76 normally developing chick embryos were divided into a control group of 39 and a radiation group of 37, with the radiation group exposed to mobile phone radiation for 10 hours each day from day 4 to 19 of incubation. Behavioral tests were performed 4 days after hatching, followed by T2-weighted MR imaging and diffusion tensor imaging. Compared with controls, chicks in the radiation group showed significantly slower aggregation responses, lower belongingness, and weaker vocalization. No significant differences were found for brain size and structural maturity except for cerebellum size, which was significantly smaller in the radiation group. The hatching and heteroplasia rates did not differ significantly between groups.

Long-term electromagnetic exposure of developing neuronal networks: A flexible experimental setup.

Oster S et al. · 2016

Researchers developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide for controlled, reproducible exposure of developing neuronal networks in vitro. The Stripline showed very good performance in the 800-1000 MHz band, with simulations indicating uniform specific absorption rate values of 361.7 ± 11.4 mW/kg at 1 W, 900 MHz across a large exposure area. Thermal drift during exposure did not exceed 0.1 K in the cellular medium. Embryonic rat cortical neurons were cultured on microelectrode array chips for non-invasive electrophysiological assessment over several weeks, demonstrating the system's reliability for long-term studies.

Evidence of cellular stress and caspase-3 resulting from a combined two-frequency signal in the cerebrum and cerebellum of sprague-dawley rats.

López-Furelos A et al. · 2016

Forty rats were divided into four groups of ten. Twenty-four hours after exposure, HSP 90 and HSP 70 levels differed between cerebral hemispheres at high SAR, while caspase-3 did not. In cerebellar hemispheres, single RF exposure at high SAR altered HSP 90, HSP 70, and caspase-3 compared with controls. The combined signals did not produce additive effects on absorbed energy or biological outcomes.