Meo SA et al. · 2011
Forty male Wistar albino rats were divided into three groups: a control group of eight, a group exposed for 30 minutes/day, and a group exposed for 60 minutes/day for a total period of 3 months. Testicular morphological changes were examined under a light microscope. The 60-minute exposure group showed 18.75% hypospermatogenesis and 18.75% maturation arrest compared to controls, while the 30-minute group showed no abnormal findings.
Lukac N et al. · 2011
Researchers exposed bovine semen to 1800 MHz GSM-like RF-EMW in vitro and measured motility at 0, 30, 120, and 420 minutes using a computer-assisted semen analyzer. The percentage of motile and progressively motile sperm decreased significantly after 120 and 420 minutes of exposure. Average path distance decreased after 30 and 420 minutes, while path velocity initially increased at 30 minutes but decreased at 420 minutes. Amplitude of lateral head displacement also decreased significantly after 420 minutes.
Kumar S, Kesari KK, Behari J. · 2011
Researchers exposed male Wistar rats to a 2.45 GHz microwave field for two hours per day over 60 days. They also applied a 100 Hz pulsed electromagnetic field to some groups, either alone or combined with the microwave exposure. Microwave exposure significantly increased caspase and creatine kinase levels and decreased testosterone and melatonin. Pulsed electromagnetic field exposure relieves the effect of microwave exposure by inducing Faraday currents.
Gutschi T et al. · 2011
Researchers examined 2110 men attending an infertility clinic from 1993 to October 2007 and divided them into cell phone users and non-users. Cell phone users had a significantly higher percentage of pathological sperm morphology (68.0%) compared to non-users (58.1%). Cell phone users also showed significantly higher testosterone and lower luteinizing hormone levels, while follicle stimulating hormone and prolactin levels did not differ significantly.
Saygin M, Caliskan S, Karahan N, Koyu A, Gumral N, Uguz A · 2011
Researchers used twelve-week-old male Wistar Albino rats divided into cage control, sham control, and 2.45 GHz EMR groups, with the exposure group receiving 3.21 W/kg for 60 minutes daily over 28 days. The exposed rats showed a significant reduction in Leydig cell numbers compared to cage controls. Spermatogenesis scores differed significantly between groups, and Bax gene and Caspase-8 enzyme levels showed significant changes.
Kumar S, Kesari KK, Behari J. · 2011
Falzone N, Huyser C, Becker P, Leszczynski D, Franken DR. · 2011
Highly motile human spermatozoa were exposed for 1 hour to 900-MHz mobile phone radiation at a specific absorption rate of 2.0 W/kg and examined at various times after exposure. The radiation did not affect the sperm acrosome reaction, but significant reductions were found in sperm head area and acrosome percentage of the head area among exposed sperm compared with unexposed controls. The mean number of zona-bound sperm was 22.8 in exposed samples versus 31.8 in controls, a statistically significant difference (p < 0.05). The researchers concluded that these results could indicate a significant effect of RF-EMF on sperm fertilization potential.
Kesari KK, Kumar S, Behari J. · 2011
The study investigated the effect of mobile phone exposure on fertility-related markers in 70-day-old male Wistar rats. Rats were exposed in Plexiglas cages for 2 hours a day for 35 days, with a specific absorption rate estimated at 0.9 W/kg. In exposed rats, antioxidant enzymes glutathione peroxidase and superoxide dismutase decreased, while malondialdehyde and free radical generation increased significantly. The researchers concluded that radiofrequency electromagnetic waves from commercially available cell phones might affect the fertilizing potential of spermatozoa.
Takahashi S et al. · 2010
Researchers exposed pregnant rats to 2.14 GHz electromagnetic fields for 20 hours per day during gestation and lactation at low and high specific absorption rates. No abnormal findings were observed in the dams, the F1 generation, or the F2 offspring across multiple parameters including growth, organ weights, survival, development, hormonal status, memory function, reproductive ability, embryotoxicity, and teratogenicity. The authors concluded that under their experimental conditions, whole-body exposure to 2.14 GHz for 20 hours per day during gestation and lactation did not cause any adverse effects on pregnancy or the development of rats.
Lee HJ et al. · 2010
Male rats were exposed to 848.5 MHz RF for 12 weeks. The exposure schedule consisted of two 45-min RF exposure periods separated by a 15-min interval. The whole-body average SAR was 2.0 W/kg. Researchers measured sperm counts, malondialdehyde concentrations, spermatogenesis stage frequency, germ cell counts, apoptotic cells, and performed immunoblotting for p53, bcl-2, caspase 3, p21, and PARP. The study concluded that subchronic exposure did not have observable adverse effects on rat spermatogenesis.
Falzone N, Huyser C, Franken DR, Leszczynski D. · 2010
Researchers examined effects of mobile phone radiation on apoptosis-related properties in human spermatozoa. Ejaculated, density-purified, highly motile human spermatozoa were exposed to mobile phone radiation at specific absorption rates of 2.0 and 5.7 W/kg. Mobile phone radiation had no statistically significant effect on any of the parameters studied, including caspase 3 activity, externalization of phosphatidylserine, DNA strand breaks, and reactive oxygen species generation. This suggests that impairment of fertility reported in some studies was not caused by induction of apoptosis in spermatozoa.
Elliott P et al. · 2010
The study was a case-control study using cancer registry and national birth register data in Great Britain with 1397 cases aged 0-4 and 5588 controls matched by sex and date of birth. Mean distance from a macrocell base station, total power output within 700 m, and modelled power density at the birth address were similar for cases and controls. Adjusted odds ratios for all cancers were 1.01 (0.87-1.18) for intermediate and 1.02 (0.88-1.20) for highest modelled power density exposure, with no significant trends for brain and central nervous system cancers or leukaemia and non-Hodgkin's lymphoma.
Vrijheid M et al. · 2010
A birth cohort was established in Sabadell, Spain between 2004 and 2006. Mothers completed questions about cell phone use in week 32 of pregnancy (n = 587) and their children's neurodevelopment was tested at 14 months using the Bayley Scales of Infant Development (n = 530). We observed only small differences in neurodevelopment scores between the offspring of cell phone users and nonusers. Those of users had higher mental development scores and lower psychomotor development scores, which may be due to unmeasured confounding, and there was no trend with amount of cell phone use within users.
Salama N, Kishimoto T, Kanayama HO, Kagawa S · 2010
Researchers randomly assigned 18 male rabbits to a phone group exposed to an 800 MHz mobile phone in standby for 8 hours daily over 12 weeks and a control group. Copulatory behavior was recorded with six female teasers, and serum testosterone, dopamine, and cortisol were measured. The phone group showed significantly more mounts without ejaculation, significantly fewer mounts with ejaculation, increased biting or grasping, longer mounting latency, and no significant hormone differences.
Salama N, Kishimoto T, Kanayama HO · 2010
Sperm concentration dropped significantly in the phone group by week 8 compared to both control groups. Motile sperm percentage declined significantly after week 10 in the phone and stress groups, with a greater decline in the phone group. Seminiferous tubule diameter was significantly smaller in the phone group than in either control group.
Meo SA, Al-Drees AM, Husain S, Khan MM, Imran MB · 2010
Researchers used 34 male Wistar albino rats divided into a control group and two groups exposed to mobile phone radiation for 30 or 60 minutes daily for three months. The 60-minute daily exposure group showed a significant decrease in serum testosterone compared to controls (p=0.028).
Fragopoulou AF, Koussoulakos SL, Margaritis LH. · 2010
Physically comparable pregnant mice were exposed to GSM 900 MHz radiofrequency radiation from a mobile phone. Within 5h after birth most pups were fixed and processed with double staining and paraffin histology, while other pups remained with their mothers until teeth eruption. Exposed newborns appeared normal externally, but histological examination revealed variations in cranial bone and rib ossification and displacement of Meckelian cartilage.
Chavdoula ED, Panagopoulos DJ, Margaritis LH. · 2010
Researchers exposed Drosophila melanogaster to GSM-900MHz radiation for 6 minutes daily to compare continuous and intermittent exposure effects on reproductive capacity and apoptotic cell death. Previous work showed 6-minute continuous daily exposure for five days greatly reduced reproductive capacity, measured by F1 pupae count, and this decrease was non-thermal and linked to increased DNA fragmentation in egg chamber cells. The current experiments found intermittent exposure also reduced reproductive capacity and disrupted the actin-cytoskeleton network of egg chambers, with the effect also due to DNA fragmentation. Intermittent exposures with 10-minute intervals were almost as effective as continuous exposure, while longer intervals allowed the flies to partly recover.
Panagopoulos DJ, Margaritis LH · 2010
Researchers exposed fruit flies to GSM 900 and DCS 1800 radiation for daily durations of 1 to 21 minutes. Reproductive capacity decreased almost linearly as exposure duration increased for both radiation types. GSM 900 radiation was slightly more bioactive than DCS 1800 radiation at the same exposure durations and equal intensities.
Panagopoulos DJ, Margaritis LH · 2010
Researchers examined whether the bioactivity windows for GSM 900 and 1800 MHz radiation depend on field intensity by measuring reproductive capacity in Drosophila melanogaster. They compared insects exposed at the window distances (30 cm for GSM 900, 20 cm for GSM 1800) to insects exposed closer (8 cm and 5 cm) behind a metal grid that shielded microwave and ELF fields while keeping intensities roughly equal. The decrease in reproductive capacity did not differ significantly between the two exposure groups. The bioactivity window seems to be due to the intensity of radiation-field (10 microW/cm(2), 0.6-0.7 V/m) at those distances.
Panagopoulos DJ, Chavdoula ED, Margaritis LH · 2010
Researchers exposed adult Drosophila melanogaster to GSM 900/1800 mobile phone antenna radiation at distances from 0 to 100 cm and measured reproductive capacity and cell death in the gonads using the TUNEL assay. The radiation decreased reproductive capacity by inducing cell death at all distances tested. The effect was strongest at 20-30 cm (radiation intensity around 10 microW/cm(2)), diminished considerably beyond 40-50 cm, and was not evident beyond 1 m or below 1 microW/cm(2).
Grigoriev YG et al. · 2010
Researchers replicated earlier Soviet studies from 1974 to 1991 that had examined immunological and reproductive effects of long-term low-level radiofrequency exposure in rats. Wistar rats were exposed in the far field to 2450 MHz continuous wave RF fields at an incident power density of 5 W/m² for 7 hours per day, 5 days per week, for a total of 30 days, resulting in a whole-body SAR of 0.16 W/kg. The results partly confirmed the earlier findings and indicated possible effects from non-thermal RF exposure on autoimmune processes. A separate part of the study suggested possible adverse effects of blood serum from exposed rats on pregnancy and foetal development of intact rats, though the researchers noted that applying these results to exposure standards is limited.
Balmori A. · 2010
Researchers exposed eggs and tadpoles of the common frog (Rana temporaria) to electromagnetic radiation from mobile phone antennae located 140 meters away for two months, from the egg phase until an advanced tadpole phase. The exposed group of 70 tadpoles showed low coordination of movements, asynchronous growth, and 90% mortality. A control group of 70 tadpoles kept under the same conditions inside a Faraday cage showed normal movement coordination, synchronous development, and only 4.2% mortality. The researchers concluded that radiation from phone masts in a real situation may affect development and may cause an increase in mortality of exposed tadpoles.
Kesari KK, Kumar S, Behari J. · 2010
Researchers exposed male Wistar rats to mobile phone frequencies at 0.9 W/kg specific absorption rate for 2 hours per day over 35 days. A significant decrease in protein kinase C and total sperm count, along with increased apoptosis, were observed in the exposed rats. The results suggest that a reduction in protein kinase activity may be related to overproduction of reactive oxygen species under microwave field exposure.
Kesari KK, Behari J. · 2010
Sperm GPx and SOD activity showed a significant decrease in exposed rats, while catalase showed a significant increase compared with the control group. The percentage of apoptosis was higher in the exposed group, and significant decreases were found in the S phase and G2/M transition phase of the cell cycle. The researchers concluded that these radiations may have a significant effect on the reproductive system of male rats, which may be an indication of male infertility.
