Maes A, Verschaeve L, Arroyo A, De Wagter C, Vercruyssen L · 1993
Researchers exposed human peripheral blood lymphocytes to 2450 MHz microwaves for 30 and 120 minutes at constant body temperature. Exposure caused a marked increase in chromosome aberrations, including dicentric chromosomes and acentric fragments, and micronuclei. Microwave exposure did not influence cell kinetics or sister chromatid exchange frequency.
Field AS, Ginsburg K, Lin JC · 1993
Researchers studied the effects of pulsed microwaves on membrane input resistance and action potential interval statistics in spontaneously active ganglion neurons of land snails (Helix aspersa). Experiments were conducted at strictly constant temperature, so macroscopic heating was not responsible for any observed effects. Exposure significantly increased mean input resistance, while mean firing rate and mean input current were not affected. The net input current was found to be more variable in exposed neurons, though changes in interspike interval standard deviation and input current autocorrelation were not consistent across neurons.
Seaman RL, DeHaan RL · 1993
Researchers studied inter-beat intervals of aggregated cardiac cells from chicken embryos during 190-second exposures to 2.45 GHz microwaves. The inter-beat interval decreased during exposures at 42.0 W/kg and higher with continuous-wave or square-wave modulation, consistent with thermal effects. Increases occurred during continuous-wave exposures at 1.2-12.2 W/kg and decreases after pulse-modulated exposures at 8.4-12.2 W/kg, which were not consistent with simple thermal effects.
Somosy Z, Thuroczy G, Kovacs J · 1993
Researchers investigated pyroantimonate-precipitable calcium in mouse intestinal epithelial cells after whole-body exposure to 2450 MHz continuous and 16 Hz square-modulated microwaves. Immediately after modulated irradiation at 0.5 and 1 mW/cm², calcium deposits rapidly appeared on the cytoplasmic side of lateral membranes and in the junctional complex including tight junctions. These changes were reversible, with calcium distribution returning to control-like patterns after 24 hours. Continuous-wave exposure at the same energy did not alter calcium distribution.
Rotkovska D, Bartonickova A, Kautska J · 1993
Lange DG, D'Antuono ME, Timm RR, Ishii TK, Fujimoto JM. · 1993
Chou CK, Guy AW, Kunz LL, Johnson RB, Crowley JJ, Krupp JH · 1992
Two hundred male Sprague-Dawley rats were assigned in equal numbers to radiation-exposure and sham-exposure conditions, with exposure beginning at 8 weeks of age and continuing daily for 25 months. The experimental rats were exposed to 2,450-MHz pulsed microwaves at 800 pps with a 10-microseconds pulse width, square-wave modulated at 8-Hz. Blood samples were analyzed at regular intervals for serum chemistries, hematological values, protein electrophoretic patterns, thyroxine, and plasma corticosterone levels.
Lai H, Carino MA, Horita A, Guy AW, · 1992
Researchers investigated which opioid receptor subtypes mediate a microwave-induced decrease in central cholinergic activity in rats. Rats were pretreated with specific antagonists of mu, delta, and kappa opioid receptors before exposure to microwaves. All three receptor subtypes were involved in the decrease in cholinergic activity in the hippocampus.
Lai H, Carino MA, Horita A, Guy AW · 1992
Researchers exposed rats to single 45-minute and repeated daily 45-minute microwave sessions at 2450 MHz, 1 mW/cm², with a whole-body SAR of 0.6 W/kg. After a single exposure, receptor concentration increased in the cerebral cortex but not in the hippocampus or cerebellum. No significant change in binding affinity of the receptors was observed in any of the brain regions studied. After repeated exposures, no significant change in receptor concentration was found in the cerebral cortex. Handling and exposure procedures did not significantly affect benzodiazepine receptors.
Kues HA et al. · 1992
Researchers exposed anesthetized monkeys to pulsed 2.45 GHz microwaves after topical treatment with timolol maleate or pilocarpine. Drug pretreatment lowered the power-density threshold for corneal endothelial lesions and increased iris vascular permeability from 10 to 1 mW/cm². Effects occurred at an average SAR of 0.26 W/kg and were confirmed with specular microscopy, fluorescein angiography, and horseradish peroxidase tracer techniques.
Czerska EM, Elson EC, Davis CC, Swicord ML, Czerski P · 1992
Researchers exposed human lymphocytes in vitro for 5 days to continuous-wave (CW) or pulsed-wave (PW) 2450-MHz radiation at non-heating and heating levels, with actual average SARs ranging to 12.3 W/kg. At non-heating levels, CW exposure did not affect spontaneous lymphoblastoid transformation, but PW exposure enhanced it significantly (P less than .002). At heating levels, PW exposure enhanced transformation to a greater extent than either conventional heating or CW heating, a finding significant at the .02 level.
Phelan AM, Lange DG, Kues HA, Lutty GA · 1992
Researchers exposed B16 melanoma cells to 2.45-GHz pulsed microwaves at 10 mW/cm² for 1 hour and measured membrane ordering using electron paramagnetic resonance. The exposure shifted the cell membrane from a more fluid-like phase to a more solid, ordered state. Similar membrane ordering occurred in melanin-reconstituted liposomes, but not in amelanotic cells or liposomes without melanin. Superoxide dismutase inhibited the microwave-induced ordering, implicating oxygen radicals as a cause.
Inaba R, Shishido K, Okada A, Moroji T. · 1992
Maillefer RH, Quock RM · 1992
Male Swiss Webster mice were exposed to 10, 15, and 20 mW/cm2 RFR at 2450 MHz for 10 minutes at SARs of 23.7, 34.6, and 45.5 W/kg, then tested in the abdominal constriction paradigm. Confinement alone did not appreciably alter body temperature but appeared to induce stress-associated analgesia that was not blocked by naltrexone. RFR exposure raised body temperature and further increased analgesia in an SAR-dependent manner. The high SAR-induced analgesia was blocked by naltrexone, but the hyperthermia was not.
Fukui Y, Hoshino K, Inouye M, Kameyama Y · 1992
Researchers exposed pregnant mice to 2.45 GHz microwave radiation for 15 or 20 minutes on day 13 of gestation. Pyknotic cell incidence in the ventricular zone was 1.83% after 15 minutes and 3.06% after 20 minutes of microwave exposure. Microwave radiation for 20 minutes produced effects comparable to immersion in 42°C hot water for 15 minutes. Brain weight was significantly lower and neuron density higher in the 20-minute exposure group compared to controls. The researchers concluded that microwave radiation at the tested dose mainly has a thermal effect.
Veyret B et al. · 1991
French researchers exposed mice to low-power pulsed microwaves (similar to radar frequencies) for 10 hours daily over five days to test effects on immune system function. They found that simple pulsed signals had little effect, but when the signals included additional amplitude modulation, the mice showed significant changes in antibody production - some frequencies strengthened immune responses while others weakened them.
Ciaravino V, Meltz ML, Erwin DN · 1991
Researchers exposed Chinese hamster ovary cells to adriamycin and pulsed RFR at 2,450 MHz and 33.8 W/Kg for 2 h. The maximal temperature in the tissue-culture medium was 39.7 +/- 0.2 degrees C. The experiments were controlled for chemical and RFR exposures as well as for temperature. Verified statistically, the data indicate that the RFR did not affect changes in cell progression caused by adriamycin and did not change the number of sister chromatid exchanges induced by adriamycin.
Garson OM, McRobert TL, Campbell LJ, Hocking BA, Gordon I. · 1991
Researchers studied chromosome damage in 38 radio-linemen exposed to radio-frequency radiation and 38 matched controls. They analyzed Two hundred metaphases per person, scored blind, and found the rate of aberrant cells was the same in both groups. There were no statistically significant differences in the types of chromosome aberrations.
Lai H, Carino MA, Wen YF, Horita A, Guy AW · 1991
Rats were repeatedly exposed to pulsed, circularly polarized microwaves at 2,450 MHz with 2-microsecond pulses at 500 pps, a power density of 1 mW/cm², and a whole-body SAR of 0.6 W/kg. Ten 45-minute sessions increased muscarinic cholinergic receptor concentration in the hippocampus, while ten 20-minute sessions decreased concentration in the frontal cortex and hippocampus. Pretreatment with naltrexone at 1 mg/kg IP before each exposure session blocked these microwave-induced changes in cholinergic receptors.
Lange DG, Sedmak J · 1991
Microwave exposure produced a dose-dependent increase in JEV-induced lethality. Hypercarbia at 5, 10, and 20% CO2 also produced a dose-dependent increase in JEV-induced lethality.
Somosy Z, Thuroczy G, Kubasova T, Kovacs J, Szabo LD · 1991
Researchers exposed mouse embryo 3T3 cells to 2450 MHz continuous and 16 Hz square-modulated microwaves at absorbed energies from 0.0024 to 2.4 mW/g. Modulated waves caused more morphological changes than continuous waves at the same intensity. Cell surface negative charge decreased after modulated-wave exposure but was unchanged after continuous-wave exposure at the same dose. Modulated waves at 0.024 mW/g increased cell ruffling and caused cytoplasmic ultrastructural alterations, while continuous waves required higher doses of 0.24 and 2.4 mW/g for similar effects.
Saalman E et al. · 1991
Researchers studied how 2.45 GHz microwave radiation affects the permeability of unilamellar liposomes containing a fluorescent marker. After 10 minutes of exposure, microwave-treated liposomes showed significantly greater marker release than those heated conventionally. The experiments were conducted above the lipid membrane's phase transition temperature, unlike earlier studies.
Krause D, Mullins JM, Penafiel LM, Meister R, Nardone RM, · 1991
Researchers studied the effects of 2.45-GHz continuous-wave microwaves on interferon-regulated enzymes in murine L929 cells. Radioactive 2-5A binding to RNase L was 14.5% above control in sham-exposed samples and 36.4% above control in microwave-exposed samples. RNase L specific activity rose along with the increased 2-5A binding, but 2-5A synthetase specific activity showed no change. No detectable differences were noted in cell viability, plating efficiency, or proliferation rate after exposure.
Spiers DE, Baummer SC · 1991
Researchers exposed Japanese quail eggs to 2,450 MHz microwaves at 5 or 20 mW/cm² for 8 hours daily from day 1 to 15 of incubation. At 30 °C ambient temperature, microwave exposure increased wet-embryo mass on day 16 by 9% at 5 mW/cm² and 61% at 20 mW/cm² compared to predicted masses without microwaves. Post-treatment tests showed no reliable indication that repeated microwave exposure resulted in abnormal physiological development. The authors concluded that microwave radiation can increase egg temperature and embryonic growth rate at below-normal incubation temperatures without altering basic metabolic and thermal characteristics.
Balcer-Kubiczek EK, Harrison GH. · 1991
Researchers exposed actively growing cultures of C3H/10T1/2 cells for 24 hours to 2.45-GHz microwaves pulse-modulated at 120 Hz. The electromagnetic fields alone had no effect on cell survival or induction of neoplastic transformation. When combined with the tumor promoter TPA, the enhancement of transformation was highly significant and ranged up to a level equivalent to that produced by 1.5 Gy of X rays. The frequency of neoplastic transformation was dependent on the level of electromagnetic exposure and was additive with doses of X rays given as a cocarcinogen.
