Misa Agustiño MJ et al. · 2012
Fifty-four adult female Sprague-Dawley rats were individually exposed to 2.45 GHz radiation for 30 minutes in a GTEM cell at non-thermal SAR levels. Ninety minutes after exposure HSP-90 and HSP-70 decreased significantly at SAR values of 0.046±1.10 W/Kg and 0.104±5.10−3 W/Kg. Twenty-four hours after exposure HSP-90 had partially recovered and HSP-70 had recovered completely. There were few indications of lesions in the glandular structure and signs of apoptosis were negative in all radiated animals.
Unknown authors · 2011
The Health Council of the Netherlands examined whether exposure limits should shift from specific absorption rate to a maximum allowable temperature increase. The council evaluated this change across six criteria including consistency, applicability, and causality. The council concluded that changing the dosimetric quantity would not advance the debate on non-thermal effects from long-duration, low-intensity exposure. The council recommended keeping the current system of basic restrictions and reference levels expressed as SAR and in V/m or µT.
Unknown authors · 2011
The Health Council of the Netherlands examined whether electromagnetic field exposure limits should shift from measuring SAR (specific absorption rate) to measuring temperature increases. They concluded that maintaining current SAR-based limits is preferable and that changing the measurement approach wouldn't resolve scientific debates about non-thermal EMF effects.
Saygin M, Caliskan S, Karahan N, Koyu A, Gumral N, Uguz A · 2011
Researchers used twelve-week-old male Wistar Albino rats divided into cage control, sham control, and 2.45 GHz EMR groups, with the exposure group receiving 3.21 W/kg for 60 minutes daily over 28 days. The exposed rats showed a significant reduction in Leydig cell numbers compared to cage controls. Spermatogenesis scores differed significantly between groups, and Bax gene and Caspase-8 enzyme levels showed significant changes.
Kumar S, Kesari KK, Behari J. · 2011
Researchers exposed male Wistar rats to a 2.45 GHz microwave field for two hours per day over 60 days. They also applied a 100 Hz pulsed electromagnetic field to some groups, either alone or combined with the microwave exposure. Microwave exposure significantly increased caspase and creatine kinase levels and decreased testosterone and melatonin. Pulsed electromagnetic field exposure relieves the effect of microwave exposure by inducing Faraday currents.
Sakurai T et al. · 2011
The study used DNA microarray to examine gene expression in SVGp12 human glial cells after exposure to 2.45 GHz continuous-wave RF fields at SARs of 1, 5, and 10 W/kg for 1, 4, and 24 hours. Microarray analysis identified 23 assigned and 5 non-assigned gene spots as potentially altered. Follow-up RT-PCR analysis of Twenty-two genes showed no significant alterations in gene expression. The authors concluded that under their experimental conditions they found no evidence that RF field exposure affected gene expression in SVGp12 cells.
Sakurai T et al. · 2011
Japanese researchers exposed human brain cells (glial cells) to 2.45 GHz radiofrequency radiation at various power levels for up to 24 hours and examined whether this changed gene activity. Using advanced genetic analysis techniques, they found no significant changes in how genes were expressed in the exposed cells compared to unexposed controls. This suggests that RF radiation at these levels did not trigger detectable genetic responses in this type of brain cell.
Türker Y et al. · 2011
Thirty male Wistar Albino rats were divided into five groups, with three groups receiving 2.45-GHz radiation for 60 minutes per day over 28 days. Radiation exposure increased lipid peroxidation and decreased vitamins A, C, and E in heart tissue.
Türker Y et al. · 2011
Researchers exposed rats to 2.45-GHz radiation (the same frequency used by Wi-Fi and microwaves) for one hour daily over 28 days and found it caused oxidative stress in heart tissue. The study showed that supplements selenium and L-carnitine could partially protect against this damage by reducing harmful free radicals and supporting the body's natural antioxidant defenses. This suggests that common wireless frequencies may stress cardiovascular tissue at the cellular level.
Unknown authors · 2011
Researchers studied mitotic spindle disturbances and apoptosis activation in V79 Chinese hamster cells exposed to continuous 2.45 GHz microwaves to investigate non-thermal cell damage. Exposure for 15 minutes significantly increased the proportion of aberrant spindles and apoptotic cells and decreased the mitotic index compared with untreated cells. Control heating experiments showed that spindle assembly was unaffected up to 41°C and apoptosis occurred only at 40°C, a temperature higher than that reached during microwave exposure.
Unknown authors · 2011
Researchers exposed Chinese hamster cells to 2.45 GHz microwave radiation (the same frequency used in microwave ovens and WiFi) and found it damaged the cellular machinery responsible for cell division and triggered cell death. The damage occurred at non-thermal power levels, meaning it wasn't caused by heating but by the electromagnetic fields themselves.
Papageorgiou CC et al. · 2011
Fifteen male and fifteen female subjects, matched for age and education level, were investigated while performing a modified version of the Hayling Sentence Completion test adjusted to induce working memory. ERPs were recorded at 30 scalp electrodes, both without and with exposure to a Wi-Fi signal. Male subjects showed decreased P300 amplitudes compared to female subjects, but only in the presence of Wi-Fi EMF. The researchers concluded that Wi-Fi exposure may exert gender-related alterations on neural activity associated with attentional resources during a linguistic working-memory test.
Unknown authors · 2011
In part B, mice were transplanted intraperitoneally with ascites (Ehrlich-Lettre ascites, strain E) carcinoma cells and then exposed to 112 MHz amplitude-modulated radiation at 16 Hz and 2.45 GHz radiation separately for 14 days. No tumor development occurred in mice exposed to radiofrequency or microwave radiation after a single topical dose of DMBA. Mice transplanted with Ehrlich-Lettre ascites carcinoma cells and exposed to the fields showed a slight but insignificant increase in cell numbers compared to controls.
Ibitoye ZA, Aweda AM. · 2011
Gasmelseed A. · 2011
They used a plane multilayered method at wireless frequencies of 900, 1800, and 2450 MHz. The study calculated specific absorption rate in three different eye models. Results confirmed a complex relationship between absorption and structural variations of the eye at these frequencies.
Garaj-Vrhovac V et al. · 2011
The study examined workers occupationally exposed to pulsed microwave radiation from marine radars. Comet assay parameters (mean tail intensity 0.67 vs 1.22, moment 0.08 vs 0.16) and micronucleus test parameters showed statistically significant differences between exposed and unexposed groups. Glutathione concentration was significantly lower in exposed workers (1.24 vs 0.53) while malondialdehyde concentration was significantly higher (1.74 vs 3.17), indicating oxidative stress. Electromagnetic field strength was measured at marine radar frequencies of 3 GHz, 5.5 GHz, and 9.4 GHz with specific absorption rate values determined.
Chaturvedi CM et al. · 2011
Jorge-Mora T et al. · 2011
Researchers investigated the effects of 2.45 GHz microwave radiation on the paraventricular nucleus of rat hypothalamus using c-Fos protein expression as a marker of cellular activation. Rats were exposed once or repeatedly (ten times in two weeks) to 2.45 GHz radiation at power intensities of 3 and 12 W in a GTEM cell, with SAR determined by Finite Difference Time Domain calculation. High SAR increased c-Fos expression at 90 minutes and 24 hours post-exposure, low SAR increased c-Fos counts at 24 hours, and repeated 3 W exposure increased PVN activation by more than 100% compared to acute exposure and non-radiated controls.
Sambucci M et al. · 2011
Newborn mice were exposed to WiFi signals at SAR of 0.08 or 4 W/kg, 2 hours per day, 5 days per week, for 5 weeks starting the day after birth. No effects were seen on most immune parameters in either sex, but male mice exposed at 4 W/kg showed reduced IFN-γ production in spleen cells. The researchers concluded that early post-natal WiFi exposure does not induce detrimental effects on the developing immune system. The study used a blind procedure with sham controls and found no differences in body weight or development between groups.
Yang X, He G, Hao Y, Chen C, Li M, Wang Y, Zhang G, Yu Z · 2010
Researchers studied how electromagnetic field exposure affects N9 microglial cells and the role of the JAK2-STAT3 signaling pathway in any resulting pro-inflammatory responses. EMF significantly induced phosphorylation of JAK2 and STAT3, and also dramatically increased the expression of CD11b, TNF-α, and iNOS, and the production of NO. When cells were pretreated with the JAK inhibitor P6, phosphorylation of JAK2 and STAT3 was strongly suppressed, and expression of CD11b, TNF-α, and iNOS was suppressed at 12 hours but not at 3 hours after EMF exposure.
Kesari KK, Behari J, Kumar S. · 2010
Researchers exposed male Wistar rats to 2.45 GHz microwave radiation for 2 hours a day for 35 days at a power density of 0.34 mW/cm2. The whole brain tissue was dissected and used for study of double strand DNA breaks by micro gel electrophoresis and comet assay. Exposed brain cells showed a significant increase in comet head, tail length, and tail movement. The study concluded that chronic exposure to these radiations may cause significant damage to brain, which may be an indication of possible tumour promotion.
Unknown authors · 2010
Fragopoulou et al · 2010
Sambucci M et al. · 2010
Researchers exposed pregnant mice to WiFi signals for 2 hours daily over 14 consecutive days starting 5 days after mating. No effects were found on mating success, number of newborns per mother, or body weight at birth. Offspring showed no differences in spleen cell number, B-cell frequency, or antibody serum levels. B cells from all groups produced comparable IgM and IgG and proliferated similarly when challenged with LPS in vitro. These findings were consistent in both female and male offspring at juvenile and adult ages.
Grigoriev YG et al. · 2010
Researchers replicated earlier Soviet studies from 1974 to 1991 that had examined immunological and reproductive effects of long-term low-level radiofrequency exposure in rats. Wistar rats were exposed in the far field to 2450 MHz continuous wave RF fields at an incident power density of 5 W/m² for 7 hours per day, 5 days per week, for a total of 30 days, resulting in a whole-body SAR of 0.16 W/kg. The results partly confirmed the earlier findings and indicated possible effects from non-thermal RF exposure on autoimmune processes. A separate part of the study suggested possible adverse effects of blood serum from exposed rats on pregnancy and foetal development of intact rats, though the researchers noted that applying these results to exposure standards is limited.
