Syed Taha SMA et al. · 2025
Male Sprague Dawley rats (n = 6 per group) were exposed for 60 days to 3.5 GHz or 24 GHz RF-EMF for 1 h/day or 7 h/day, and testicular cytokines, apoptosis-related genes, and sperm quality were evaluated. IL-10 was significantly reduced in the 24 GHz group at both 1-h and 7-h exposure duration, and at 7 h TNF-α was also lower at 24 GHz. Casp3 expression was higher and Tp53 was lower at 3.5 GHz at 1-h exposure duration. Sperm concentration and viability were reduced after 24 GHz exposure at 7 h, while sperm motility was reduced after 3.5 GHz exposure at both durations.
Unknown authors · 2025
Researchers exposed adult zebrafish to Wi-Fi radiation (2.45 GHz) for 4 hours daily over 30 days and found significant behavioral problems, movement changes, reduced brain chemicals, and increased oxidative stress. This study adds to growing evidence that chronic Wi-Fi exposure may harm brain function in vertebrates.
Unknown authors · 2025
Researchers established a dual-frequency EMR mouse model at 0.8/2.65 GHz. Exposure to this dual-frequency EMR significantly induced anxiety-like behavior in mice. Molecular experiments showed a significant decrease in CB1R levels in the medial prefrontal cortex along with a notable reduction in the endogenous cannabinoids 2-arachidonoylglycerol and anandamide, indicating downregulation of the entire endocannabinoid system. Overexpressing and knocking down CB1R in the medial prefrontal cortex confirmed that increasing mPFC CB1R levels reduced anxiety-like behavior while decreasing mPFC CB1R levels exacerbated it. Dual-frequency EMR also induced changes in the endocannabinoid system in the basolateral amygdala, and female mice exhibited similar behavioral phenotypes and molecular mechanisms.
Unknown authors · 2025
Nineteen healthy participants with a mean age of 36.5 years were exposed to 5G mobile phone exposure at 3.6 GHz and 0.0030 W/m2 for 5 and 20 minutes. Corticospinal excitability, intracortical facilitation, short intracortical inhibition, and long intracortical inhibition were measured with transcranial magnetic stimulation before and after exposure. A repeated-measures ANOVA found no significant interactions between exposure condition and time for any of these measures (all p > 0.15). Bayesian analyses gave Bayes factors close to 1, indicating inconclusive evidence for both the null and alternative hypotheses.
Unknown authors · 2025
Researchers created a 3D digital twin of an adult fruit fly using micro-CT scans and used it in numerical simulations to estimate absorbed power at 3.6 GHz. They exposed adult Drosophila melanogaster to 3.6 GHz fields for 5 days at 5.4 to 9 V/m (3.56 to 9.88 nW absorbed) and measured locomotor activity. They also exposed flies for 48 hours at an absorption level of 1.91 mW and measured fecundity. No effects were found on either locomotor activity or fecundity.
Gelenli Dolanbay E et al. · 2025
Researchers exposed pregnant rats to 3.5 GHz radiofrequency radiation (similar to 5G frequencies) and found that their male offspring developed severe testicular damage by adulthood. The exposed rats showed reduced sperm production, increased DNA damage, and higher rates of cell death in reproductive tissues compared to unexposed controls.
Özyılmaz C et al. · 2025
Syed Taha SMA et al. · 2025
Researchers exposed male rats to 5G frequencies (3.5 GHz and 24 GHz) for 60 days and found significant damage to sperm quality and testicular function. Both frequencies reduced sperm motility and concentration, with 24 GHz causing more severe effects including immune system disruption. The damage worsened with longer daily exposure times.
Vijay S et al. · 2025
Histological examination revealed vacuolation in the testes and corpus epididymis of both exposed groups and multifocal atypical hyperplasia in the seminal vesicle. Seminiferous tubule diameter decreased gradually in both exposed groups with a substantial decrease in the 24-hour group, and the spermatogenesis index was significantly reduced in both exposed groups. Sperm concentration was significantly reduced in both exposed groups, sperm motility decreased significantly in the 4-hour group, and sperm viability increased substantially in the 24-hour group.
Lameth J et al. · 2025
This study examined the effects of repeated head exposures to 5G signals at 3.5 GHz on adult male mice. The researchers found that while the 5G exposures did not alter behavioral outcomes, they did produce modifications in gene expression within the cerebral cortex.
Özyılmaz C et al. · 2025
Researchers exposed pregnant rats to WiFi-frequency radiation (2.45 GHz) throughout pregnancy, then examined their offspring's thyroid glands one year after birth. The study found significant thyroid damage including increased cell death, DNA breaks, tissue scarring, and abnormal cells in the exposed offspring. This suggests prenatal WiFi exposure may cause lasting thyroid problems that persist into adulthood.
Zhaowen Z et al. · 2025
Researchers randomly assigned 7-8-week-old male C57BL/6 mice to a sham group or a 4.9 GHz RFR group (16 mice per group) and assessed anxiety, depression, cognition, sperm quality, and fertility in their male offspring. Paternal RFR exposure induced anxiety-like behaviors and impaired sperm quality in the first-generation male offspring. No significant effects were observed on depression-like behaviors, cognitive performance, or fertility across F1-F2 generations.
Unknown authors · 2025
Unknown authors · 2025
EMR exposure caused marked structural damage to seminiferous tubules, increased collagen deposition, expanded tubular and interstitial volumes, raised IL-6 and TNF-α expression, and reduced antioxidant enzyme activities while increasing MDA levels. Alpha-lipoic acid supplementation prevented the structural damage, lowered inflammatory markers, restored AR, ZO-1, and ZO-2 expression, and reversed the oxidative stress parameters.
Laván D et al. · 2025
The researchers indirectly analyzed the relationship between oxidative-stress-responsive genes after Wi-Fi 2.4 GHz exposure and genes linked to Alzheimer’s disease. They reported that prolonged exposure to this radiation could worsen the modification of key neurodegeneration genes such as GSK3B and APOE. The authors note that the link between wireless systems and Alzheimer’s disease remains inconclusive and requires further research.
Unknown authors · 2024
Researchers investigated the effects of 4.9 GHz radiofrequency field on emotional behaviors and spatial memory in adult male mice. They used open field test, tail suspension test, and Y maze to assess anxiety, depression-like behavior, and spatial memory. Anxiety-like behavior and spatial memory did not change after exposure, but depression-like behavior was induced. Neuron number decreased and pyroptosis increased in the amygdala but not the hippocampus.
Unknown authors · 2024
Previous research found increased wakefulness in mice after prolonged exposure to 2.4 GHz electromagnetic radiation with 100 Hz square-pulsed modulation at 1/8 duty cycle. The current study addresses whether modulation frequency, pulse edge sharpness, carrier frequency, and duty cycle matter for sleep and wakefulness alteration.
Unknown authors · 2024
Researchers evaluated the impact of 5G-modulated 3.5 GHz RF EMF on mitochondrial stress in human fibroblasts and keratinocytes exposed for 24 hours at specific absorption rates of 0.25, 1, and 4 W/kg. They found a statistically significant reduction in mitochondrial ROS in fibroblasts at 1 W/kg, a slight but statistically significant enhancement of UV-B effects in keratinocytes at 0.25 and 1 W/kg, and no effect on mitochondrial membrane potential or apoptosis in any cell type or exposure condition.
Torres-Ruiz M et al. · 2024
Researchers exposed zebrafish embryos to unmodulated 700 MHz and 3500 MHz radiofrequency radiation for 1 and 4 hours during the blastula period and assessed outcomes up to 120 hours post fertilization. The exposure did not affect mortality, hatching, or body length, but caused specific organ morphological changes and lasting behavioral alterations in activity, anxiety-like behavior, and habituation. Effects were stronger at 700 MHz, particularly after 4 hours of exposure, and acetylcholinesterase activity was reduced.
Unknown authors · 2024
Researchers exposed SH-SY5Y neuronal-like cells and peripheral blood mononuclear cells to 2.45 GHz EMR for 24 hours with sulforaphane at 5, 10, and 25 µg/mL. Higher sulforaphane concentrations caused harmful effects and the compound showed a hormetic response in Nrf2, SOD2, and apoptosis markers.
Unknown authors · 2024
Researchers exposed brain-like cells and immune cells to 2.45 GHz WiFi radiation for 24 hours, finding it reduced cell survival and caused cellular damage. However, a low dose of sulforaphane (a compound from broccoli) protected the cells from this radiation damage. The protective effect only worked at low doses - higher concentrations of sulforaphane actually made things worse.
Unknown authors · 2024
Unknown authors · 2024
Researchers studied the impact of 2850 MHz electromagnetic field radiation on red and green cabbage seedlings, exposing them daily for seven days to homogeneous EMF-r for one, two, or four hours. Exposure caused dose-dependent reductions in root length, shoot length, and biomass in both cultivars compared with controls. Chlorophyll and carotenoid contents decreased significantly in both red and green cabbage. Phenolic, flavonoid, and anthocyanin contents increased with exposure duration in both cultivars. EMF-r produced oxidative stress shown by dose-dependent increases in total antioxidant activity, DPPH activity, H2O2 content, and MDA content, while antioxidant enzyme activities increased significantly.
Unknown authors · 2024
Researchers exposed 30 Aedes aegypti eggs to radiofrequency radiation at baseline, 900 MHz, and 18 GHz, with each frequency tested in triplicate. They assessed hatching responses, development times, larval numbers, and pupation periods until adult emergence. The 900 MHz group showed the highest hatching rate at 79 ± 10.54% and the lowest adult emergence rate at 33 ± 2.77%, with a significant difference between groups (p = 0.03).
Jamal et al. · 2024
Researchers conducted a randomized, triple-blind, crossover study with 44 healthy young volunteers exposed to 3.5 GHz signals at 1 to 2 V/m. Each session included baseline, exposure, and postexposure phases with seven runs of electrodermal activity recordings stimulated by beeps and skin temperature measurements at the hand, head, and neck. The study suggests a slight increase in head and neck temperature and minimal modulation of electrodermal metrics after exposure, though results remain within normal physiological range and could reflect an uncontrolled variable.
