RAMAN SPECTROSCOPY OF MAMMALIAN CELLS
Unknown authors
The study investigated Raman spectroscopy to distinguish tumor cells from normal cells but encountered fluorescence, Rayleigh scattering, and cell settling in liquid media. They identified phosphate buffered saline (PBS) as a nonfluorescent medium suitable for Raman spectroscopy. Suspending cells in agarose immobilized them, eliminated intensity fluctuations, and produced consistent spectra. Preliminary spectra of BHK cells in agarose showed possible Raman lines below 200 cm⁻¹.