Unknown authors · 2021
Researchers exposed T98 and A172 glioblastoma cells to 50 Hz, 70 G electromagnetic fields combined with temozolomide. The combination enhanced cell death and decreased migration potential in both cell lines. The treatment also increased p53 gene expression, decreased cyclin-D1 protein, and led to MGMT protein expression in T98 cells but not in A172 cells.
Unknown authors · 2021
Pregnant Wistar albino rats were exposed to pulsed 2.45 GHz microwave radiation at 2.8 mW/cm² for 2 hours daily throughout pregnancy. Male offspring were not exposed after birth and were examined at 75 days of age. Exposed adult rats showed irregular seminiferous tubules, degenerated germ cells, reduced tubule diameter and epithelial height, and increased interstitial space. Exposed rats also had more vacuoles, altered organelles, necrotizing cells, empty spaces between Sertoli and germ cells, and increased lipid droplets in Leydig cells. Fluoro Jade-C and superoxide dismutase 2 positivity were increased in exposed rats.
Mustafa E, Luukkonen J, Makkonen J, Naarala J · 2021
The researchers investigated the effects of 50 Hz extremely low-frequency magnetic fields on circadian gene expression, DNA damage signaling, and DNA repair after bleomycin treatment. Murine FDC-P1 hematopoietic cells were exposed to 200 μT magnetic fields for 15 minutes, 2 hours, 12 hours, or 24 hours. Immediate DNA damage was measured with a comet assay and circadian rhythm gene expression with real-time PCR. Circadian rhythm-related genes were upregulated after 12 hours and downregulated after 24 hours of exposure, but core circadian genes were not affected. The repair rate for bleomycin-induced DNA damage decreased only after 24 hours of magnetic field exposure.
Unknown authors · 2021
Unknown authors · 2021
Both ELF-EMFs and microampere currents significantly reduced long-term potentiation. The effect of 0.1 μA current was similar to 100 Hz/2 mT ELF-EMFs, while 0.2 μA current had a stronger effect than 200 Hz/2 mT ELF-EMFs on high-frequency stimulation LTP.
Unknown authors · 2020
Researchers exposed young adult male Wistar rats to mobile phone radiofrequency electromagnetic field radiation at 1966.1 MHz, 4.0 mW/cm2, and 0.36 W/kg for 2 hours per day over 16 weeks. Another set of animals was kept under similar conditions without radiation exposure as controls. At the end of the exposure period, the rats were tested for fear memory and then euthanized to measure hippocampal oxidative stress, circulating pro-inflammatory cytokines, and stress hormones. Exposed rats showed significantly increased hippocampal oxidative stress, elevated levels of IL-1beta, IL-6, and TNF-alpha, increased adrenal gland weight, and higher levels of ACTH and corticosterone. However, changes in contextual fear memory were not statistically significant.
Unknown authors · 2020
Researchers exposed rats to mobile phone radiation (1966.1 MHz) for 2 hours daily over 16 weeks and found significant increases in brain oxidative stress, inflammatory markers, and stress hormones. The study demonstrates that chronic cell phone radiation exposure can trigger biological stress responses in the brain and body, even at levels similar to everyday phone use.
Unknown authors · 2020
The study investigated molecular events associated with the synergistic activity of PEMFs and BMP2 on osteogenic differentiation. Human MSCs were exposed to PEMFs at 75 Hz and 1.5 mT in combination with BMP2 at the minimal dose able to induce differentiation. Researchers analyzed changes in BMP signaling pathway gene expression and phosphorylation of downstream proteins including SMAD1/5/8 and MAPK. Results showed synergistic activity on osteogenic differentiation transcription factors and markers, with increased BMP2, BMP6, and BMP type I receptor gene expression and activation of SMAD1/5/8 and p38 MAPK.
Gulati et al. · 2020
Researchers compared genotoxic effects of three UMTS frequency channels used by 3G phones (1923, 1947.47, and 1977 MHz) in human lymphocytes. Human lymphocytes were exposed for 1 hour and 3 hours and tested for ROS, DNA damage, TP53 mutations, preleukemic fusion genes, and apoptosis. No effects were seen on ROS, apoptosis, preleukemic fusion genes, or TP53 mutations at any frequency, though bulk RNA expression was inhibited. A small but statistically significant increase in DNA damage occurred depending on the UMTS frequency channel, with the maximum effect at 1977.0 MHz.
Gulati et al. · 2020
Researchers exposed human immune cells to three different 3G cell phone frequencies (1923, 1947, and 1977 MHz) for 1-3 hours to test for DNA damage and cellular stress. They found small but significant DNA damage that varied by frequency, with 1977 MHz causing the most harm, while other cellular damage markers remained unchanged.
Unknown authors · 2020
Vafaei S, Motejaded F, Ebrahimzadeh-Bideskan A · 2020
Unknown authors · 2020
Ashta A , Motalleb G , Ahmadi-Zeidabadi M · 2020
Researchers investigated the effects of a 10 Hz, 50 G frequency magnetic field, a 50 G static magnetic field, and 200 μm temozolomide on viability, free radical production, and p53 expression in human A172 glioblastoma cells. The combination of temozolomide with both magnetic fields increased cytotoxicity, free radical production, and p53 gene and protein expression.
Unknown authors · 2020
Jeong et al. · 2020
Public concerns exist about deleterious biological consequences of radiofrequency electromagnetic fields. These concerns have led to wide consideration of the potential effects of RF-EMF on the central nervous system.
Jeong et al. · 2020
Researchers exposed mice to 1,950 MHz radiofrequency radiation (similar to cell phone frequencies) for an extended period and observed behavioral changes along with alterations in gene expression patterns. The study focused on potential effects to the central nervous system, finding measurable impacts on both mouse behavior and genetic activity. This adds to growing evidence that chronic RF exposure may influence brain function and cellular processes.
Unknown authors · 2020
Pregnant rats were exposed to radiofrequency fields starting on day 21 of pregnancy, and offspring were tested with Y maze at 3 weeks, open field at 7 weeks, and hippocampal NMDAR expression at 9 weeks. The 1800 MHz and 1800 MHz + WiFi groups showed a downward trend in body weight, and the WiFi group opened eyes earlier than controls. In open field tests, the 1800 MHz + WiFi group had shorter overall path length and delayed stationary time, the WiFi group had shorter path length and increased velocity in the error arm, and the 1800 MHz + WiFi group showed increased central area activity. Both exposure groups down-regulated NR2A and NR2B and up-regulated NR2D, NR3A, and NR3B, while the WiFi group also up-regulated NR1 and NR2C.
Unknown authors · 2020
Researchers exposed pregnant rats to 1800 MHz cell phone radiation and 2400 MHz WiFi signals, then tested their offspring's behavior and brain development. The exposed pups showed altered movement patterns, changed brain chemistry, and different developmental timing compared to unexposed controls. The study suggests prenatal EMF exposure may affect how the brain develops during critical early periods.
Hepatic injury induced by radio frequency waves emitted from conventional Wi-Fi devices in Wistar ratsH M Fahmy et al. · 2020
Researchers exposed Wistar female rats to a standard 2.45 GHz radio frequency source with an averaged whole-body specific absorption rate of 0.01 W kg−1 24 h−1 daily for 40 consecutive days. The rats were randomly divided into control and Wi-Fi–exposed groups. Liver samples were evaluated for oxidative stress parameters, transaminase levels, molecular structure using Fourier transform infrared spectroscopy, histopathology, and ultrastructure. Exposure led to severe oxidative stress, deleterious effects on liver function, altered molecular structure, and severe histological and ultrastructural alterations indicating hepatotoxic effects.
Unknown authors · 2020
Researchers randomly divided 120 Wistar rats into ten groups of 12 and exposed them to RF-EMF, 10 cGy x-rays, 8 Gy x-rays, combinations of these, or sham exposure. They measured liver enzymes in serum, as well as histopathological changes and oxidative stress markers including MDA, nitric oxide, antioxidant power, total thiols, and PCO. Gamma-glutamyltransferase was not associated with irradiation, but aspartate transaminase, alanine transaminase, and alkaline phosphatase showed some association. MDA and PCO levels increased after 8 Gy irradiation, but pre-exposure to RF-EMF modulated these changes, and lobular inflammation frequency was associated with the intervention type.
Unknown authors · 2020
Pregnant mice were exposed to Wi-Fi signal (2.4 GHz) for 2 and 4 hours. Placenta tissues were examined for MDA and SOD levels, and gene expression of SOD, CDKNA1, GADD45a, Bax, and Bcl-2 was measured by real-time PCR, while apoptosis was assessed using TUNEL assay. MDA and SOD levels significantly increased in exposed groups (P-value<0.05), and SOD mRNA expression also significantly increased. CDKN1A and GADD45a gene expression increased in exposed groups (P-value<0.05), and apoptosis increased as shown by quantitative PCR and TUNEL assay (P-value<0.05).
Unknown authors · 2020
Researchers exposed pregnant mice to Wi-Fi signals (2.4 GHz) for 2 and 4 hours and examined the placenta tissue. They found increased oxidative stress, DNA damage markers, and cell death in the placenta after Wi-Fi exposure. This matters because the placenta is crucial for healthy fetal development during pregnancy.
Unknown authors · 2020
Researchers aimed to demonstrate the effect of ELF-EMF on selected chemical components of honeybees using FTIR spectroscopy. Honeybees were exposed to 50 Hz EMF at 1.6 mT for durations of 2, 6, 12, 24, and 48 hours. FTIR analysis revealed that exposure longer than 2 hours induced changes in the structure of chemical compounds, especially in regions corresponding to DNA, RNA, phospholipids, and protein vibrations, compared to unexposed controls.
Unknown authors · 2020
Researchers investigated chromosome response in human neuronal-like cells and plant roots (Allium sativum and Vicia faba) exposed to static and 50 Hz magnetic fields at intensities from 1 mT to 0.8 T. FTIR spectroscopy showed a significant decrease in phosphate band intensity in DNA after exposure of neuronal-like cells to 1 mT static and 50 Hz fields, suggesting chromatin uncoiling and unpackaging. Microscopy directly observed this effect in Allium sativum and Vicia faba roots exposed to a 0.8 T static magnetic field.
