Duong CN, Kim JY · 2016
Researchers studied whether EMF exposure could protect a human microglial cell line, HMO6, from cell death caused by oxygen-glucose deprivation (OGD), a model of ischemic injury. HMO6 cells were cultured for 4 hours under OGD with or without exposure to EMF at different combinations of frequencies and intensities, and cell survival, intracellular calcium, and ROS levels were measured. Among the combinations tested, 50 Hz/1 mT EMF was the most potent at reducing OGD-induced cell death and lowering intracellular calcium and ROS levels. A significant but less potent protective effect was also found at 10 Hz/1 mT, while no protective effect was found at other combinations.
Kesari KK, Juutilainen J, Luukkonen J, Naarala J. · 2016
(E) Barthélémy A et al. · 2016
Researchers exposed male Sprague Dawley rats for 15 minutes at 0, 1.5, or 6 W/kg, or for 45 minutes at 6 W/kg, to radiofrequency electromagnetic fields. Memory, emotionality, and locomotion were tested using fear conditioning, the elevated plus maze, and the open field. Long-term memory was reduced and cytosolic GFAP was increased in the hippocampus and in the olfactory bulb at 6 W/kg. No MBP or Aβ1–40 expression modification was shown. The study suggests that RF EMF-induced astrogliosis had functional consequences on memory but did not demonstrate that it was secondary to neuronal damage.
Hu Y et al. · 2016
Researchers exposed genetically modified mice with Alzheimer's disease to extremely low frequency magnetic fields (50Hz, 500μT) for three months daily. The magnetic field exposure improved cognitive function, reduced brain cell death, and decreased tau protein abnormalities that are hallmarks of Alzheimer's disease. This suggests that controlled magnetic field exposure might have therapeutic potential for neurodegenerative conditions.
Hidisoglu E et al. · 2016
Researchers exposed rats to 2100-MHz electromagnetic fields (similar to 3G cell phone frequencies) for 2 hours daily, comparing short-term (1 week) versus long-term (10 weeks) exposure effects on brain function and oxidative stress. They found that short-term exposure actually appeared protective, improving visual processing speed and antioxidant defenses, while long-term exposure caused harmful effects including slower brain responses and increased oxidative damage.
Unknown authors · 2016
Researchers studied the mutagenic effect of radiofrequency electromagnetic field exposure using the micronucleus test in secondary roots of Vicia faba seedlings. Roots were exposed for 72 hours using a continuous wave of 915 MHz radiation at three power density levels, with specific absorption rate values falling in the range of 0.4 to 1.5 W/kg. Three independent experiments showed a significant increase in micronucleus frequency, from a 2.3-fold increase at the lowest SAR level up to a 7-fold increase at the highest SAR.
Hidisoglu E et al. · 2016
Researchers exposed rats to 2100-MHz electromagnetic fields (similar to 3G cell phone frequencies) for either 1 week or 10 weeks and measured brain function and oxidative stress. Short-term exposure actually improved brain processing speed and antioxidant defenses, while long-term exposure caused brain processing delays and increased oxidative damage. The findings suggest that duration of EMF exposure determines whether effects are protective or harmful.
Calabrò E. · 2016
Researchers exposed human SH-SY5Y neuronal-like cells to a 50 Hz magnetic field and examined protein changes using Fourier Transform Infrared Spectroscopy. After exposure, the vibration bands of the methylene group increased significantly, and a significant shift to low energies of the Amide I band was observed. An increase in the intensity of parallel and antiparallel β-sheet structures relative to the α-helix component was also observed after exposure. The authors interpreted these results as suggesting that both protein aggregation and increased hydrogen bonding occurred in the exposed neuronal-like cells.
Ji Y, He Q, Sun Y, Tong J, Cao Y. · 2016
Researchers preexposed mouse bone-marrow stromal cells to 900-MHz radiofrequency fields at 120 μW/cm2 for 4 hours per day for 5 days, then exposed some cells to 1.5 Gy gamma radiation. Radiofrequency exposure alone produced no significant differences in DNA strand breaks compared to control cells. When radiofrequency preexposure was followed by gamma radiation, the number of DNA strand breaks was significantly decreased and repair was faster compared to gamma radiation alone.
Feng B, Qiu L, Ye C, Chen L, Fu Y, Sun W. · 2016
Researchers exposed human amniotic epithelial cells to a 50-Hz magnetic field at 0.4 mT and examined mitochondrial permeability, mitochondrial membrane potential, and cytochrome c release. Exposure to the magnetic field at 0.4 mT for 60 min transiently induced mitochondrial permeability transition and Cyt-c release, although there was no significant effect on mitochondrial membrane potential. Cells exposed to the magnetic field showed increased intracellular reactive oxidative species levels and GSK-3β dephosphorylation, and the induced mitochondrial permeability transition was attenuated by a ROS scavenger or GSK-3β inhibitor.
Salunke BP, Umathe SN, Chavan JG. · 2016
Researchers exposed mice to 50 Hz magnetic fields (the same frequency as power lines) for up to 120 days and found it caused significant anxiety-like behavior. The study identified that these fields disrupt brain chemistry by affecting NMDA receptors and increasing glutamate levels in key brain regions. This suggests that long-term exposure to power-frequency magnetic fields can alter brain function and behavior.
Gläser K et al. · 2016
Researchers irradiated human hematopoietic stem cells (HSC) and HL-60 cells at GSM (900 MHz), UMTS (1,950 MHz), and LTE (2,535 MHz) frequencies, at intensities ranging from 0 to 4 W/kg SAR, for short and long exposure periods. Studied endpoints included apoptosis, oxidative stress, cell cycle, DNA damage, and DNA repair. In all but one endpoint, no clear effect of mobile phone radiation was detected; the only alteration was a small but statistically significant decrease in DNA damage in HSC exposed to GSM modulation for 4 hours.
Mortazavi SM et al. · 2016
The study investigated the effects of radiofrequency radiation from GSM mobile phones on insulin release in rats. RF radiation did not alter insulin release in the rats. Mild to severe inflammatory changes in the portal spaces of the liver and damage to the cells of the islet of Langerhans were observed. The changes were linked to the duration of exposure.
Çeliker M et al. · 2016
Researchers exposed rats to 2100 MHz radiofrequency radiation (similar to 3G cell phone signals) for 30 days to study effects on hearing. While the rats' hearing tests showed no functional changes, microscopic examination revealed increased cell death and degeneration in the brain areas that process sound. This suggests that cell phone radiation may damage auditory neurons even before hearing loss becomes detectable.
Kuybulu AE et al. · 2016
Researchers exposed male Wistar rats pre- and post-natally to 2.45 GHz wireless electromagnetic fields. The study included pre-natal, post-natal, and sham exposure groups. Spot urine NAG/creatinine ratio was significantly higher in both pre- and post-natal groups, and tubular injury was detected in most post-natal specimens. Pre-natal exposure showed low Bax and high Bcl-2 staining in kidney cortex and medulla, with higher Bcl2/Bax ratios than sham.
Falone S et al. · 2016
Researchers investigated whether hyperproliferation induced by a 50 Hz, 1 mT ELF magnetic field in SH-SY5Y human neuroblastoma cells is supported by improved defense against methylglyoxal and by rewiring of energy metabolism. The ELF magnetic field interfered with the biology of these malignant cells by further de-differentiating the cellular phenotype and increasing proliferative activity. The magnetic field also triggered cytoprotective mechanisms, including enhanced defense against methylglyoxal and more efficient management of metabolic energy, presumably to support rapid cell outgrowth.
Nirwane A, Sridhar V, Majumdar A · 2016
Nirwane A, Sridhar V, Majumdar A. · 2016
Researchers exposed zebrafish to cell phone radiation at levels similar to those from mobile phones (1.34 W/kg SAR) for one hour daily over two weeks. The fish showed increased anxiety-like behaviors, impaired learning ability, and brain damage from oxidative stress. This study demonstrates that even brief daily exposure to mobile phone radiation can alter brain function and damage brain cells.
Nirwane A, Sridhar V, Majumdar A · 2016
Researchers exposed zebrafish to cell phone radiation (GSM 900 MHz) for 14 days at human-equivalent levels. The fish developed increased anxiety, reduced social behavior, and impaired learning, plus brain oxidative stress indicating cellular damage. This suggests everyday cell phone radiation may affect brain function.
Paknahad M, Mortazavi SM, Shahidi S, Mortazavi G, Haghani M · 2016
Researchers prepared class V cavities in 20 extracted human premolars and randomly divided them into two groups of 10. The experimental group was exposed to 2.4 GHz Wi-Fi radiation for 20 minutes at a distance of 30 cm while the router communicated with a laptop 20 meters away. Mercury concentration in artificial saliva was significantly higher in the exposed group (0.056 ± .025 mg/L) than in the control group (0.026 ± .008 mg/L) (P =0.009).
Erkut A et al. · 2016
Pregnant rats were exposed to 1800 MHz electromagnetic field for six, 12, or 24 hours daily for 20 days, and offspring were evaluated at 60 days after birth. Increasing prenatal exposure duration significantly reduced resting cartilage levels and increased apoptotic chondrocytes and myocytes. Calcineurin activity decreased in bone and muscle, and femur, tibia, and ulna development was impaired, especially with 24-hour daily exposure.
Mugunthan N et al. · 2016
Researchers exposed mice to 2G mobile phone radiation (900-1800 MHz) for 48 minutes daily over six months and examined their brain tissue under a microscope. They found that the radiation caused significant changes in the hippocampus (the brain's memory center), including reduced numbers of neurons in key areas and smaller cell nuclei throughout the region. These cellular changes suggest that chronic mobile phone radiation exposure may damage the brain structures responsible for learning and memory.
Valbonesi P, Franzellitti S, Bersani F, Contin A, Fabbri E. · 2016
Researchers exposed rat PC12 cells to a 1.8 GHz 217-GSM signal at 2 W/kg SAR for 24 hours. AChE enzyme activity was measured by Ellman's method, mRNA by real-time PCR, and protein by Western blotting. AChE enzymatic activity increased 1.4-fold in exposed cells, while mRNA and protein expression were not affected. The authors report this as the first evidence of RF-EMF effects on AChE activity in mammalian cells at this SAR.
Nakayama M, Nakamura A, Hondou T, Miyata H · 2016
Researchers measured DNA single-strand breaks, cell viability, and nitric oxide production in macrophage RAW264 cells exposed to a 0.5-mT, 50-Hz magnetic field for 24 hours with or without LPS stimulation. The magnetic field alone did not alter nitric oxide production. The combined effect of LPS and magnetic field was not mediated by LPS-induced nitric oxide production.
Unknown authors · 2016
The study evaluated the protective effect of lotus seedpod procyanidins against extremely low frequency electromagnetic field exposure at 50 Hz, 8 mT for 28 days. LSPCs increased the organ index of mice and restored damaged blood-producing function and cytokine levels including INF-γ, TNF-α, IL-2, IL-6, and IL-10 in the spleen to normal. LSPCs also inhibited splenocyte stagnation in G0/G1 phase, promoted proliferation, reduced apoptosis, prevented DNA content decline, and reversed changes in Bcl-2, Bax, Caspase-3, and Caspase-9 expression.
