Bourdineaud JP et al. · 2017
Researchers exposed Eisenia fetida earthworms to electromagnetic fields at 900 MHz and field levels ranging from 10 to 120 V m-1 for a period of two hours. All exposure treatments induced significant DNA modifications, and the number of probe hybridisation sites showed a significant two-fold decrease even after 24 hours of recovery. Genes involved in general stress, oxidative stress, and immune defence were up-regulated after exposure, and Western blots confirmed an increased quantity of HSP70 and MTCO1 proteins.
Zhang D, Zhang Y, Zhu B, Zhang H, Sun Y, Sun C · 2017
Researchers enrolled 186 male workers with occupational exposure to high-voltage electricity lines and 154 male subjects with insignificant exposure as a reference control. Resveratrol or placebo was given as dietary supplements at 500 mg twice daily, and inflammatory biomarkers and biomarkers of oxidative stress were assessed. Workers who had long-term exposure to high-voltage electricity lines showed elevated urinary levels of 8-OHdG and F2-isoprostane compared to the reference group. Resveratrol significantly reversed the adverse impacts of ELF-EMF, and stimulated cytokine production by resveratrol was found in exposed workers but not in the reference group.
Solek P et al. · 2017
Researchers investigated the effects of pulsed and continuous electromagnetic fields on two mouse spermatogenic cell lines, GC-1 spg and GC-2 spd, in vitro. The researchers evaluated mitochondrial metabolism, morphology, proliferation rate, viability, cell cycle progression, oxidative stress balance, and regulatory proteins. The results strongly suggested that EMF induced oxidative and nitrosative stress-mediated DNA damage, resulting in p53/p21-dependent cell cycle arrest and apoptosis. The researchers concluded that electromagnetic field impairs male fertility by inducing p53/p21-mediated cell cycle arrest and apoptosis.
Höytö A, Herrala M, Luukkonen J, Juutilainen J, Naarala J. · 2017
Researchers exposed human SH-SY5Y neuroblastoma cells to a 50 Hz, 100 μT magnetic field with or without a non-phototoxic level of blue light for 24 hours. The magnetic field increased cytosolic superoxide production, while blue light suppressed that effect. Mitochondrial superoxide production was reduced by both the magnetic field and blue light, but these effects were not additive. Micronucleus frequency was not affected by blue light or the magnetic field alone, but blue light, significantly when combined with the magnetic field, enhanced menadione-induced micronuclei.
Hanini R, Chatti A, Ghorbel SB, Landoulsi A. · 2017
Researchers studied the protective role of superoxide dismutase (SOD) against a static magnetic field of 200 mT in wild-type and mutant strains of Pseudomonas aeruginosa lacking one or both SOD enzymes. Inactivation of the sodM and/or sodB genes increased the sensitivity of the bacteria to stress from the static magnetic field. Mutant strains also showed significantly higher levels of malondialdehyde, a marker of oxidative damage to lipids, compared to wild-type cells.
Giorgi G et al. · 2017
Researchers exposed human neural cells (BE(2)C) to an extremely low frequency pulsed magnetic field (50 Hz, 1 mT) for 24 and 48 hours, alone and combined with oxidative stress, to study DNA methylation changes. Exposure to the magnetic field alone or oxidative stress alone produced only weak changes in DNA methylation levels at different CpG sites. When the two exposures were combined, a significant decrease in DNA methylation levels was found at different CpG sites, and most of those changes were transient.
Dornelles EB et al. · 2017
Researchers tested the in vitro cytotoxic effects of a 5 mT static magnetic field on human blood cells carrying different variants of the Val16Ala polymorphism in the manganese superoxide dismutase gene. Cytotoxic effects were observed in AA cells at all exposure times, while AV cells showed higher mortality only after 6 hours, and higher apoptosis induction was seen in AA cells compared to VV and AV cells. The authors suggest the results indicate a toxicogenetic effect of the static magnetic field related to an imbalance in SOD2 activity.
Unknown authors · 2016
Researchers exposed human lens epithelial cells to a 50 Hz ELF MF at 0.4 mT or sham exposure. DNA damage was assessed using γH2AX foci formation assay, western blot, flow cytometry, and alkaline comet assay. No significant increase in γH2AX foci formation was observed after exposures up to 48 hours, γH2AX expression levels did not differ between exposure groups, and no chromosomal DNA fragmentation was detected.
Unknown authors · 2016
They analyzed mitotic index and micronucleus rates after exposure. No significant differences were found in mitotic index rates within or between groups. Double Dyna and double Steco configurations showed statistically significant intragroup differences in micronucleus rates. No statistically significant differences in micronucleus rates were found between groups.
Unknown authors · 2016
Researchers investigated the effects of ELF magnetic fields together with 4 chemical mutagens using the bacterial Vitotox test. A 100 μT magnetic field at 50 Hz did not damage DNA and was not mutagenic in the assay. The magnetic field also did not influence the DNA-damaging capacity of the chemical mutagens.
Sanie-Jahromi F, Saadat I, Saadat M · 2016
Researchers evaluated the effects of 50-Hz EMF at two intensities and three exposure patterns, alone or combined with cisplatin, on the mRNA levels of seven DNA-repair genes in MCF-7 and SH-SY5Y cells. In MCF-7 cells exposed to EMF alone, the examined genes tended to be down-regulated, and the 15-min on/15-min off pattern at 0.50 mT down-regulated GADD45A, XRCC1, XRCC4, Ku80, Ku70 and LIG4. When cells were co-treated with cisplatin and EMF, the cisplatin IC50 rose significantly, GADD45A mRNA increased, and XRCC4, Ku80, Ku70 and DNA-PKcs mRNA decreased.
Unknown authors · 2016
Researchers investigated the effect of a 50 Hz, 1 mT extremely low frequency electromagnetic field on MAP2 and Nestin gene expression in dermal papilla mesenchymal cells. Four experimental groups were prepared: chemical exposure, EMF exposure, combined chemical and EMF exposure, and a control with no treatment, all treated for 5 days. An additional EMF group was exposed for 14 consecutive days to assess the effect of longer exposure on neural differentiation markers Nestin and MAP2. After 14 days, MAP2 expression was higher than after 5 days and cell proliferation decreased from days 5 to 20, indicating that longer exposure promotes neural differentiation.
Mahmoudinasab H, Saadat M · 2016
NQO1 mRNA decreased immediately after exposure to the 5 min on/5 min off pattern at 0.25 mT compared to unexposed cells. NQO2 mRNA increased immediately and at two hours after exposure to the same pattern at 0.50 mT compared to unexposed cells.
Unknown authors · 2016
Researchers exposed a human keratinocyte model to 60.4 GHz millimeter waves at 20 mW/cm2 for 3 hours in athermic conditions. No keratinocyte transcriptome modifications were observed after this exposure. The glycolysis inhibitor 2-deoxyglucose decreased cellular ATP content and induced a high modification in the transcriptome affecting 632 coding genes. The combined millimeter-wave and 2-deoxyglucose exposure did not alter ATP content but slightly altered the transcriptome, and RT-PCR validation confirmed six MMW-sensitive genes.
Koyama S et al. · 2016
Researchers exposed human corneal epithelial and human lens epithelial cells to 60 GHz millimeter-wavelength radiation for 24 hours at 1 mW/cm2. The micronucleus frequency did not increase significantly compared with sham-exposed and incubator controls. The comet assay for DNA strand breaks and heat shock protein expression also showed no statistically significant effects. The researchers concluded that the radiation had no effect on genotoxicity in human eye cells.
Koyama S et al. · 2016
Researchers exposed human corneal epithelial cells to 0.12 THz radiation at 5 mW/cm2 for 24 h and then examined genotoxicity, morphological changes, and heat shock protein expression. Micronucleus frequency did not increase significantly in exposed cells compared with sham-exposed and incubator controls, whereas the positive control treated with bleomycin for 1 h showed a significant increase. No significant morphological changes were observed, and expression of Hsp27, Hsp70, and Hsp90α did not differ significantly among the three treatment groups.
Unknown authors · 2016
Researchers studied the mutagenic effect of radiofrequency electromagnetic field exposure using the micronucleus test in secondary roots of Vicia faba seedlings. Roots were exposed for 72 hours using a continuous wave of 915 MHz radiation at three power density levels, with specific absorption rate values falling in the range of 0.4 to 1.5 W/kg. Three independent experiments showed a significant increase in micronucleus frequency, from a 2.3-fold increase at the lowest SAR level up to a 7-fold increase at the highest SAR.
McNamee JP et al. · 2016
Adult male C57BL/6 mice were exposed to 1.9 GHz pulse-modulated or continuous-wave radiofrequency fields for 4 hours per day over 5 days at specific absorption rates of 0, 0.2, and 1.4 W/kg. Total RNA was isolated from the auditory cortex, amygdala, caudate, cerebellum, hippocampus, hypothalamus, and medial prefrontal cortex and gene expression was analyzed using Illumina MouseWG-6 (v2) BeadChip arrays. When controlling the false discovery rate, no differentially expressed genes were identified in any brain region compared to sham controls. Some genes showed differences without multiple-comparison correction, but no consistent pattern emerged across exposure levels or modulations.
Nakatani-Enomoto S et al. · 2016
Nakayama M, Nakamura A, Hondou T, Miyata H · 2016
Researchers measured DNA single-strand breaks, cell viability, and nitric oxide production in macrophage RAW264 cells exposed to a 0.5-mT, 50-Hz magnetic field for 24 hours with or without LPS stimulation. The magnetic field alone did not alter nitric oxide production. The combined effect of LPS and magnetic field was not mediated by LPS-induced nitric oxide production.
Sagioglou NE et al. · 2016
Researchers studied apoptosis in the model biological system Drosophila melanogaster under different exposure protocols. A signal generator was used operating alternatively under Continuous Wave (CW) or Frequency Modulation (FM) emission modes, at three power output values (10 dB, 0, -10 dB), under four carrier frequencies (100, 395, 682, 900 MHz). Newly emerged flies were exposed either acutely for 6 or 60 minutes on the sixth day, or repeatedly for 6 or 60 minutes daily for the first six days of their life. All exposure protocols resulted in an increase of apoptotic cell death observed in egg chambers, even at very low electric field strengths. FM waves seem to have a stronger effect in ACD than continuous waves.
Safian F et al. · 2016
Researchers exposed mouse 2-cell embryos to EMF (900–1800 MHz) for 30 minutes per day over four days and compared them with unexposed controls. The rate of embryo survival to the blastocyst stage was similar in both groups. However, the percentage of dead embryos at the 2-cell stage was significantly higher in the EMF-exposed group, and loss of cell viability significantly increased in experimental blastocysts.
Odacı E et al. · 2016
Pregnant rats were exposed to 900 MHz EMF for 1 hour each day during days 13 to 21 of pregnancy. At 60 days old, offspring showed higher apoptotic index, greater DNA oxidation levels, and lower sperm motility and vitality compared to controls. Histopathological examination revealed immature germ cells in seminiferous tubule lumen and altered seminiferous tubule epithelium and structure. TUNEL staining showed nuclear changes indicating apoptosis with large numbers of apoptotic cells in most seminiferous tubule epithelium.
Banerjee S, Singh NN, Sreedhar G, Mukherjee S. · 2016
The study explored the effects of mobile phone radiation on micronucleus frequency in oral mucosal cells. Subjects were divided into low users (less than five years and less than three hours per week) and high users (more than five years and more than 10 hours per week). High mobile phone users showed a significantly increased number of micronucleated cells per 1000 exfoliated buccal mucosal cells compared to low users. The use of mobile phones with warmth around the ear showed the maximum increase in micronucleated cells. Mobile phone radiation even in the permissible range when used for longer duration causes significant genotoxicity, and the genotoxicity can be avoided to some extent by the regular use of headphones.
Akdag MZ et al. · 2016
The study used 16 adult male Wistar-Albino rats, with 8 exposed to 2.4 GHz radiation for over a year and 8 sham-exposed. DNA damage in brain, liver, kidney, skin, and testicular tissues was measured using the comet assay as percentage tail DNA. Percentage tail DNA increased in brain, kidney, liver, and skin of exposed rats but the increases were not statistically significant. In contrast, DNA damage in testicular tissue increased significantly (p < 0.01). The authors concluded that long-term 2.4 GHz Wi-Fi exposure does not cause DNA damage in the examined organs except testes, which appear more sensitive.
