Mahmoudinasab H, Saadat M. · 2018
Researchers measured the effects of morphine and EMF, in combination with β-lapachone, on cell growth inhibition and expression of antioxidant genes in SH-SY5Y cells. The study used 0.50 mT intensity of 50 Hz EMF under two exposure conditions: "15 min field-on/15 min field-off" and "30 min field-on continuously." Under the "15 min field-on/15 min field-off" condition, NQO1 mRNA level decreased while NQO2 expression increased. Multiple linear regression analysis indicated that NQO1 mRNA levels were associated positively with β-lapachone and negatively with EMF.
Mahmoudinasab H, Saadat M. · 2018
Researchers tested four treatment combinations on MCF-7 and SH-SY5Y cells: cisplatin alone, cisplatin with morin, cisplatin with electromagnetic field, and cisplatin combined with both morin and electromagnetic field. The electromagnetic field was applied at 50 Hz and 0.50 mT using a cycle of 15 minutes field-on followed by 15 minutes field-off. The IC50 value of cisplatin in the cisplatin-plus-morin-plus-EMF treatment was significantly higher than cisplatin alone and the cisplatin-plus-morin treatment in both cell lines. In SH-SY5Y cells, most of the antioxidant genes showed upregulation with the cisplatin-plus-EMF and cisplatin-plus-morin-plus-EMF treatments, while antioxidant gene expression patterns were similar across the four treatments in MCF-7 cells.
Hajipour Verdom B, Abdolmaleki P, Behmanesh M. · 2018
Researchers investigated the effects of a homogenous static magnetic field (SMF) on doxorubicin-induced toxicity and proliferation rate of cancer cells. SMF alone decreased cell viability and proliferation rate in MCF-7 and HFF cells, similar to doxorubicin alone. The combination of 10 mT SMF and 0.1 µM doxorubicin decreased viability and proliferation of cancer and normal cells in a synergistic manner. The findings suggest that SMF can decrease the required dose of chemotherapy drugs such as doxorubicin and thereby decrease their side effects.
Consales C et al. · 2018
Coballase-Urrutia E et al. · 2018
Researchers studied the effects of static magnetic fields at 0.8 mT on oxidative stress markers in a restraint stress animal model. Exposure to static magnetic fields over 5 days at 30, 60, and 240 minutes per day decreased NO, MDA, AGEs, and AOPP levels and increased SOD and GSH levels, with the response being time-dependent.
Unknown authors · 2017
Researchers investigated the role of PEMF exposure in regulating glutamate-induced excitotoxicity through the endocannabinoid system. PEMF exposure improved viability of HT22 cells after excitotoxicity and reduced lactate dehydrogenase release and cell death. A CB1R specific inhibitor suppressed the protective effects of PEMF exposure, even though CB1R expression did not change. PEMF exposure elevated AEA and 2-AG levels, indicating modulation of the endocannabinoid metabolic system. CB1R/ERK signaling was shown to be an important downstream pathway of PEMF in regulating excitotoxicity.
Sanie-Jahromi F, Saadat M · 2017
Luukkonen J et al. · 2017
Researchers pre-exposed human SH-SY5Y neuroblastoma cells to a 50 Hz, 100 μT magnetic field for 24 hours before treating them with menadione for 1 or 3 hours. They measured DNA damage response proteins using immunoblotting, DNA damage with the alkaline Comet assay, and cell cycle distribution with SYTOX Green staining and flow cytometry. The main findings were decreased p21 protein levels after 1-hour menadione treatment and increased G1 phase cells with decreased S phase cells after 3-hour menadione treatment. These effects were also observed without menadione. The authors concluded that magnetic field exposure can alter the G1 checkpoint response and that p21 may be involved in early responses to magnetic field exposure.
Fathi E et al. · 2017
Researchers exposed adipose tissue-derived mesenchymal stem cells to 0.432 μg/ml ZnSO4 together with a 50 Hz, 20 mT electromagnetic field. The combined treatment increased expression of osteogenic genes, alkaline phosphatase activity, and calcium levels. The effect depended on PKA and ERK1/2 signaling, as inhibitors of these pathways blocked the osteogenic promotion. The treatment also raised cAMP and PKA activity and upregulated Wnt/β-catenin pathway genes.
Unknown authors · 2017
The study evaluated the combined effect of 100 μM temozolomide and a 100 Hz, 100 G electromagnetic field on U87 and T98G human glioma cell lines. Co-treatment synergistically increased apoptosis in both cell lines by raising P53, Bax, and Caspase-3 expression and lowering Bcl-2 and Cyclin-D1 expression. The combination also increased reactive oxygen species production and overexpressed the heme oxygenase-1 gene compared to controls.
Unknown authors · 2017
Researchers investigated the effect of 1950 MHz Universal Mobile Telecommunication System radiofrequency exposure on Chinese hamster lung fibroblast V79 cells. Genotoxic and cytotoxic effects were measured using the cytokinesis-block micronucleus assay after 20-hour exposures at SAR values of 0.15, 0.3, 0.6, and 1.25 W/kg. Exposure at 0.15 and 0.3 W/kg significantly increased micronucleus frequency without cytotoxicity, while 0.6 and 1.25 W/kg had no effect. Pre-exposure at 1.25 W/kg for 20 hours before MMC treatment significantly reduced micronucleus frequency compared to MMC alone, indicating an adaptive response, whereas pre-exposure at 0.3 W/kg did not.
Talebnejad MR et al. · 2017
Researchers exposed 40 adult white New Zealand rabbits to GSM-simulated microwave radiation at distances of 10 cm or 30 cm for one or three days. Electroretinography responses measured seven days after exposure showed no statistically significant differences between any groups. Retinal tissue appeared normal without degeneration, but ciliary body congestion occurred more often in rabbits receiving higher microwave doses.
Su L, Wei X, Xu Z, Chen G · 2017
Researchers exposed neurogenic A172, U251, and SH-SY5Y cells to 1800 MHz RF-EMF at 4.0 W/kg intermittently for 1, 6, or 24 hours. DNA damage was measured by quantifying γH2AX foci, an early marker of DNA double-strand breaks. Cell cycle progression, proliferation, and viability were assessed using flow cytometry, hemocytometer, and cell counting kit-8 assay. Exposure did not significantly induce γH2AX foci formation or cause abnormal cell cycle progression, proliferation, or viability. Prolonged incubation up to 48 hours after exposure also did not significantly affect cellular behavior.
Villarini M et al. · 2017
Researchers exposed SH-SY5Y5 and SK-N-BE-2 neuroblastoma cells to 50 Hz magnetic fields at 0.01, 0.1, or 1 mT, aluminum chloride at 4 or 40 µM, or both together for 1 hour continuously or 5 hours intermittently. They measured DNA damage with the comet assay, redox status via GSH/GSSG ratio, and Hsp70 expression by western blot and real-time RT-PCR. Neither the magnetic field nor aluminum alone caused DNA damage, altered the GSH/GSSG ratio, or changed Hsp70 levels, and combined exposure showed no synergistic toxic effect.
Yang M et al. · 2017
Researchers conducted a systematic review and meta-analysis of 11 studies with 6028 cases and 11488 controls to investigate mobile phone use and glioma risk. Long-term mobile phone use of at least 10 years showed a significant positive association with glioma (OR = 1.44, 95% CI = 1.08-1.91). Long-term ipsilateral use was also associated with glioma (OR = 1.46, 95% CI = 1.12-1.92), while contralateral use was not associated regardless of duration. Long-term use was associated with 2.22 times greater odds of low-grade glioma (OR = 2.22, 95% CI = 1.69-2.92), but mobile phone use of any duration was not associated with high-grade glioma (OR = 0.81, 95% CI = 0.72-0.92).
Qureshi ST, Memon SA, Abassi AR, Sial MA, Bughio FA. · 2017
Researchers exposed dry chickpea seeds to GSM cell phone radiation at 900 MHz and laptop radiation at 3.31 GHz for 24 and 48 hours. Untreated seeds served as a negative control and gamma rays at 250 Gray served as a positive control. All radiofrequency treatments inhibited seed germination, with the 48-hour laptop exposure showing the strongest negative effect. Mitotic index decreased and abnormality index increased as exposure duration and frequency increased. Cell membrane damage, nuclear membrane damage, and ghost cells were observed only after 48 hours of exposure.
Lippi G et al. · 2017
Researchers collected two sequential citrated blood samples from 16 healthy volunteers and exposed one sample to a smartphone call for 30 minutes at 900 MHz. Exposure significantly prolonged collagen-epinephrine aggregation by a median of 10% and increased mean platelet volume by a median of 5%. Collagen-adenosine diphosphate aggregation and platelet count did not change.
D'Silva MH, Swer RT, Anbalagan J, Rajesh B. · 2017
Fertilized chick embryos were incubated in four groups: 2G exposed (60 eggs), 3G exposed (60 eggs), sham exposed control (60 eggs), and control (48 eggs). After the scheduled exposure duration, embryos were collected for histological analysis of liver structure and alkaline comet assay for DNA damage. Exposure of developing chick embryos to 2G and 3G cell phone radiations caused structural changes in liver including dilated sinusoidal spaces with haemorrhage, increased vacuolations in cytoplasm, increased nuclear diameter and karyorrhexis. DNA damage was significantly increased in embryos exposed to 2G and 3G radiation. The changes were more pronounced in the 3G experimental group.
Carlberg M, Hardell L. · 2017
The authors evaluated glioma risk from mobile and cordless phone use using Bradford Hill's viewpoints. All nine viewpoints were evaluated based on epidemiology and laboratory studies. A meta-analysis of case-control studies found an odds ratio of 1.90 (95% CI 1.31-2.76) with highest cumulative exposure. Risk increased with latency, with an OR of 1.62 (95% CI 1.20-2.19) for 10+ years latency. Highest risk was in the 20+ years latency group, OR 2.01 (95% CI 1.41-2.88) for wireless phones.
Buckner CA, Buckner AL, Koren SA, Persinger MA, Lafrenie RM. · 2017
Researchers exposed malignant cell lines to the Thomas-EMF pattern for one hour per day and observed inhibited cell growth. The same exposure did not alter growth of non-malignant cells. The anti-proliferative effect was mediated by cAMP and ERK signaling pathways. Exposure transiently altered cellular cAMP levels and promoted ERK phosphorylation in malignant cells.
Haghighat N, Abdolmaleki P, Parnian J, Behmanesh M. · 2017
Clarke D et al. · 2017
Researchers tested four different repetitive magnetic stimulation frequencies on primary neonatal astrocyte cultures and measured calcium responses. Only 1 Hz stimulation produced a statistically significant rise in intracellular calcium in both cytoplasmic and nuclear compartments. This calcium increase did not change astrocyte migration or proliferation in a scratch assay. However, 1 Hz stimulation reduced astrocyte hypertrophy 24 hours after scratch injury. The authors concluded that the results provide preliminary evidence that repetitive magnetic stimulation can influence astrocyte physiology.
Mortazavi SMJ et al. · 2017
Researchers divided 50 rats into five groups and pre-exposed three groups to 915 MHz radiofrequency radiation for 4 hours daily over one week at low, medium, and high power. Twenty-four hours after the last exposure, the rats were given a 4 Gy sublethal gamma radiation dose and sacrificed after 5 hours. Pre-exposure increased glutathione levels and glutathione reductase activity in liver, with glutathione increases only in the low power group and glutathione reductase activity increases in both low and medium power groups. The elevated antioxidant levels protected liver tissue from oxidative damage induced by the gamma radiation.
Chandel S, Kaur S, Singh HP, Batish DR, Kohli RK · 2017
Researchers exposed onion roots to 2100 MHz electromagnetic field radiation for 1, 2, and 4 hours in a single day. The exposure increased levels of malondialdehyde, hydrogen peroxide, and superoxide anion, indicating oxidative stress. Antioxidant enzymes superoxide dismutase and catalase showed increased activity in response.
Vlasova II et al. · 2017
Researchers exposed whole blood to millimeter-wave radiation at 32.9-39.6 GHz and 100 W/m² for 15 minutes and measured neutrophil oxidant production and morphofunctional changes. Neutrophil response to opsonized zymosan and E. coli was intensified after radiation exposure, while neutrophils remained intact without agonist addition. Similar enhancement occurred with isolated neutrophils in plasma, and heating was identified as the primary mechanism for increased oxidant production.
