Unknown authors · 2023
The EPG transgene was expressed in inhibitory interneurons under the hDlx promoter. Kainic acid was used to induce acute seizures. In vivo electrophysiological signals were recorded. hDlx EPG rats exhibited a significant delay in the onset of first seizure and significantly fewer seizures than controls.
Unknown authors · 2023
Thirty-six Sprague–Dawley rats were randomly divided into sham, control, and PEMFs groups, and the sciatic nerves were transected in the sham and PEMFs groups. One month later the nerve ends were reconnected in those two groups. At 4 and 8 weeks the PEMFs group showed higher sciatic functional indices, more distally regenerated axons, and larger fiber diameters compared with the sham group. Axon diameters and myelin thicknesses did not differ between groups, while BDNF and VEGF expression was higher in the PEMFs group at 8 weeks and semi-quantitative analysis revealed more BDNF, VEGF, and NF200. The authors concluded that PEMFs affect axonal regeneration after delayed repair and that upregulated BDNF and VEGF may be involved.
Unknown authors · 2023
Researchers exposed fruit flies to a magnetic field of 0.4-0.6 mT and found it extended lifespan under starvation in wild-type flies but not in cryptochrome mutants. The same exposure increased motor function in both wild-type and neurodegenerative disease model flies. Magnetic field exposure also improved night-time sleep quality in wild-type flies but not in cry<sup>b</sup> mutants. Repeated AC magnetic field exposure increased climbing activity in wild-type Drosophila but not in cry<sup>b</sup> mutants.
Unknown authors · 2023
Jin Z, Dong L, Tian L, Zhou M, Zheng Y · 2023
Researchers generated seven time-varying magnetic fields with musical rhythm from three music tracks (Turkish March, Moonlight Sonata, Funeral March) and four sinusoidal frequencies (3500 Hz, 2500 Hz, 1500 Hz, 500 Hz) at a mean intensity of about 2 mT, and recorded LTP at Schaffer-CA1 synapses using a multi-electrode array. The rhythmic magnetic fields from Turkish March and Moonlight Sonata significantly increased fEPSP amplitude, with the effect growing at higher frequencies, whereas the Funeral March field did not significantly enhance LTP. Sinusoidal magnetic fields at 3500 Hz and 2500 Hz enhanced fEPSP amplitude more than the musical rhythmic fields, while fields at 1500 Hz and 500 Hz inhibited fEPSP amplitude.
Hosseini E, Kianifard D · 2023
The study used 24 female rats divided into four groups: control, prenatal stress, ELF-EMF exposure, and combined stress plus ELF-EMF. Anxiety-like behavior was assessed with elevated plus-maze and open field tests. All treatment groups showed increased anxiety-like behavior, with the combined stress and ELF-EMF group showing the most serious anxiety. Expression of caspase-3 was upregulated and BDNF and GAP-43 were downregulated in the prefrontal cortex, dentate gyrus, and CA1 across treatment groups. Histomorphological analysis revealed extensive neurodegenerative changes in the hippocampus and prefrontal cortex.
Unknown authors · 2023
Researchers placed juvenile lumpfish in an artificial magnetic field gradient generated by a Helmholtz coil system at 230 µT for 30 minutes. Swimming speed decreased by 16% when the field was on, but overall activity and distance travelled did not change. The authors concluded that such a speed reduction near HVDC cables is unlikely to significantly affect lumpfish migration or homing.
Unknown authors · 2023
Researchers exposed adult male Wistar rats to 50 Hz extremely low-frequency magnetic fields at 1, 1.5, and 2 mT for 4 hours per day, 5 days per week for 30 days. The study found that different exposure levels caused increases in calcium levels and apoptosis in the rat brain. As the magnetic field strength increased, apoptosis and calcium levels in brain tissues also increased. The researchers concluded that the exposure caused neurodegeneration, increased apoptosis, and increased calcium concentration.
Unknown authors · 2023
Researchers tested magnetic field effects on 97,658 flies in a two-arm maze and 10,960 flies in negative geotaxis assays. Under meticulously controlled conditions with vast sample sizes, they found no evidence for magnetically sensitive behaviour in Drosophila. They suggest many or all previous positive results were false positives after reassessing statistical approaches and sample sizes. The findings cast doubt on magnetic sensing in Drosophila and support songbirds as the model for light-dependent magnetoreception.
Zhou H et al. · 2023
MF treatment significantly enhanced electricity generation and overall energy efficiency of G. sulfurreducens by 50% and 22%, respectively. The response of current to MFs was instantaneous and reversible. Cyclic voltammetry showed redox couples changed from -0.31 to -0.39 V, suggesting that MFs could alter electron transfer components. Genes for periplasmic c-type cytochromes, outer membrane cytochromes, pili, and ribosome were upregulated under MF treatment. Enhanced electron transfer was also linked to overexpression of NADH dehydrogenase I, ABC transporter, transcriptional regulation, and ATP synthase.
Zhang X-J et al. · 2023
EMP exposure induced anxiety, cognitive decline, and short-term memory impairment. The study identified 41 differentially expressed lncRNAs and 266 differentially expressed mRNAs between EMP and sham groups. Sixty-one co-expression relationships between 18 lncRNAs and 56 mRNAs were linked to synapse and metabolic pathways. A DEL-miRNA-DEM network was constructed with 51 DEL-miRNA pairs and 290 miRNA-mRNA pairs. LncRNA AABR07042999.1 and mRNAs Tph2, Slc6a4, Dbh, and Th were upregulated, and serotonin, dopamine, and norepinephrine levels increased in the prefrontal cortex and hippocampus.
Wydorski PJ et al. · 2023
Researchers collected porcine endometrial slices during the peri-implantation period and exposed them to 50 Hz ELF-EMF for 2 hours in vitro. They measured DNA methylation in the promoter regions of EGR2, HSD17B2, ID2, IL1RAP, MRAP2, NOS3, PTGER4, SERPINE1, VDR and ZFP57 using qMS-PCR. Exposure increased methylation of EGR2, ID2, and PTGER4, decreased methylation of IL1RAP and NOS3, and did not change methylation of HSD17B2, MRAP2, SERPINE1, VDR, and ZFP57.
Unknown authors · 2023
Researchers optimized an in vitro differentiation protocol of human hematopoietic stem/progenitor cells to B-cell lineages. Human HSPCs were continuously exposed to 300 mT of 50 Hz magnetic field for 35 days in a blinded manner. The percentages of myeloid or lymphoid cells and their differentiation from pro-B to immature-B cells showed no significant changes compared to the control group. Expression levels of RAG1 and RAG2 in B cells were also similar to those in the control group. Results indicate that 50 Hz magnetic field at 300 mT does not affect human B-cell early differentiation from hematopoietic stem/progenitor cells.
Şenol N, Kaya E, Coşkun Ö, Aslankoç R, Çömlekçi S · 2023
Unknown authors · 2023
Researchers assessed the effect of electromagnetic fields on honeybee pollination efficiency using field and laboratory experiments. They measured gene and protein expression in stress and behavioral pathways, and evaluated honeybee behavior and seed production in California poppy. EMF exposure caused strong physiological stress in honeybees, reduced honeybee visits and seed production in nearby poppies, and showed a hump-shaped relationship with plant community richness and abundance.
Unknown authors · 2023
Unknown authors · 2023
Researchers exposed zebrafish embryos to an 11.4 Tesla UHF from 6 to 24 hours post fertilization and assessed development over five days. No significant effects were observed on embryo mortality, hatching rate, body length, left-right patterning, or locomotor behavior. RNA sequencing revealed upregulation of TNF inflammatory factors and activation of TNF signaling pathways, which was confirmed by qPCR.
Unknown authors · 2023
Exposed MDA-MB-231 cells had higher viability, more live cells, and longer, denser filopodia than unexposed cells. ELF-MF also raised mitochondrial ROS levels and altered mitochondrial morphology in these cells. Proteomic analysis found 328 proteins changed in MDA-MB-231 cells and 242 in MCF-10A cells, with enrichment in focal adhesion and mitochondrion pathways. The exposure reduced adhesion and increased migration and invasion of MDA-MB-231 cells, while transcription factors linked to cellular reprogramming were upregulated in MDA-MB-231 and downregulated in MCF-10A.
Unknown authors · 2023
Unknown authors · 2023
Researchers investigated whether the electromagnetic perceptive gene (EPG) could serve as a therapeutic method against obesity. They confirmed that EPG modulates Ca2+ levels in primary adipose cells and regulates genes such as CasR, PPARγ, GLU4, and GAPDH during adipogenesis. The study also found that EPG changed myocyte transcription factors including CasR, MyoG, MyoD, and Myomaker in C2C12 cells and satellite cells. In vivo experiments showed that EPG with magnetic field decreased total weight, fat, and fat accumulation and increased lean mass in mice, with effects depending on age.
Huang Z, Ito M, Zhang S, Toda T, Takeda J-I, Ogi T, Ohno K · 2023
Researchers examined the effect of extremely low-frequency electromagnetic fields on AML12 and HEK293 cells. After 3 h of exposure, protein expression of HSP70 and HSP90 decreased while their acetylation increased, enhancing binding to HOP. Mitochondrial amount decreased but ATP levels and maximal oxygen consumption increased, leading to fewer protein aggregates and higher cell viability. The acetylation of HSPs returned to baseline by 12 h.
Guo et al. · 2023
Researchers exposed zebrafish embryos at 3 hours post-fertilization to a 50-Hz magnetic field at intensities of 100, 200, 400, and 800 μT for 1 hour or 24 hours daily over 5 days. Magnetic field exposure did not affect basic development parameters including hatching rate, mortality, and malformation rate. Exposure to 200 μT magnetic field significantly induced spontaneous movement hypoactivity in zebrafish larvae. Histological examination revealed brain morphological abnormalities including condensed cell nucleus and cytoplasm and increased intercellular space. Exposure to 200 μT magnetic field inhibited syn2a transcription and expression and increased reactive oxygen species levels.
Unknown authors · 2023
Eighty rats were divided into four exposure groups and a control group, and the treatment groups were exposed to 50-Hz magnetic fields at 1, 100, 500, and 2000 μT for 2 hours daily over 60 days. All rats were immunized with human serum albumin on days 31, 44, and 58. AID expression significantly decreased at 1 μT, Bcl-6 expression did not change, and serum IL-6 increased only in the 500 μT group after immunization.
Franczak A et al. · 2023
Researchers exposed HeLa, ES-2, and DU-145 cancer cells to 50 Hz ELF-EMF at 1.3 mT for 15 and 30 minutes. The exposure increased reactive oxygen species levels in the cells. ES-2 cells showed upregulation of XRCC5 and higher levels of TNF-α, RAD51, APE1, XRCC1, and NSUN2, while DU-145 cells had reduced BCL-2, HSP90, RAD51, and TNF-α and increased VIM and METTL3.
Unknown authors · 2023
The study aimed to estimate genetic alterations in proteins, isozymes, and DNA banding patterns and the extent of nuclear DNA damage in corn under electromagnetic radiation stress using SDS-PAGE, isozyme assays, RAPD-PCR, and Comet assay. SDS-PAGE analysis showed distinct polymorphisms of 96.66% between exposed and non-exposed seedlings, with band molecular weights ranging from 60.27 to 192.35 kDa and gain or loss of bands. RAPD-PCR generated 85 amplified products with polymorphism values from 90.91% to 100% and DNA fragment sizes from 153 to 1008 bp, while Comet assay recorded a tailed ratio of 20% in exposed nuclei after 5 days versus 3% in controls.