Duan Y, Wang Z, Zhang H, He Y, Lu R, Zhang R, Sun G, Sun X. · 2013
Researchers investigated whether lotus seedpod procyanidins (LSPCs) given by oral gavage could protect mice from cognitive deficits and oxidative damage caused by extremely low frequency electromagnetic field (ELF-EMF) exposure at 50 Hz and 8 mT for 28 days. A water maze test indicated that learning and memory abilities of the ELF-EMF group deteriorated significantly compared with the control group, but the ELF-EMF + LSPCs90 group had remarkably improved learning and memory abilities compared with the ELF-EMF group. ELF-EMF exposure was associated with significant increases in oxidative stress markers and decreases in antioxidant enzyme activities.
Li Y, Zhang Y, Wang W, Zhang Y, Yu Y, Cheing GL, Pan W. · 2019
Researchers allocated forty Sprague-Dawley rats to four groups, including an STZ-induced dementia group exposed to pulsed EMF at 10 mT and 20 Hz. The Morris water maze was used to assess learning and memory abilities, and IGF-1 and IGF-2 gene expression were measured by quantitative PCR. Mean escape latency was reduced by 66%, swimming distance decreased by 55%, and time for first crossing the platform decreased by 41.6% in the pulsed EMF group compared with the STZ group. IGF-2 gene expression significantly increased in the pulsed EMF group compared with the STZ group.
Akbarnejad Z et al. · 2018
Researchers injected amyloid-β 1-42 into the lateral ventricle of rats to establish an Alzheimer's disease model, then exposed some groups to a 50 Hz, 10 mT extremely low frequency magnetic field for 14 consecutive days. AD rats showed significant impairment in learning and memory compared to control rats, and spatial memory was assessed using the Morris water maze. The researchers found that the magnetic field improved learning and memory impairments and suggested it also disrupted the processes of AD rat model formation.
Hong I et al. · 2018
Researchers delivered low intensity repetitive magnetic stimulation (LI-rMS) at 10 mT and at frequencies of 1 Hz and 10 Hz to B50 rat neuroblastoma cells in vitro for 10 minutes. Both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. The effects were frequency-specific, with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites suggested that higher spontaneous activity may have led to an increase in GABA release.
Djordjevic NZ, Paunović MG, Peulić AS · 2017
Researchers investigated whether short-term exposure to ELF-EMF at 50 Hz could produce anxiety-like behaviour in rats through changes in hypothalamic oxidative stress and nitric oxide. Ten adult male Wistar albino rats were divided into a control group with no exposure and an experimental group exposed to the field during 7 days, after which open field and elevated plus maze tests measured anxiety-like behaviour. The exposed rats showed anxiety-like behaviour and higher concentrations of superoxide anion and nitric oxide in the hypothalamus, while peroxynitrite concentration was not affected.
Gao X, Wang X, Chen F, Qi H, Wang X, Ming D, Zhou P. · 2014
Researchers conducted magnetic stimulation experiments at 1 Hz and 10 mT for 20 minutes and analyzed spontaneous EEG activity from 10 subjects. Compared with a sham exposure group, EEG power in the theta band (3.5-7.5 Hz) and lower-alpha band (7.5-10 Hz) increased significantly after magnetic stimulation. In an auditory oddball task, the latency period of P300 extended and its amplitude decreased.
Glinka M, Sieroń A, Birkner E, Cieślar G · 2013
Researchers measured the activity of antioxidant enzymes and a lipid peroxidation marker in male rats exposed to an extremely low-frequency magnetic field during skin wound healing. Each animal had an experimental cut wound on its back. The 40 Hz, 10 mT exposure was associated with increased glutathione peroxidase activity in serum and decreased malone dialdehyde in liver tissue, which the researchers described as suggesting inhibition of phospholipid peroxidation and stabilization of cellular membranes.
Korpinar MA, Kalkan MT, Tuncel H. · 2012
Anxiety and stress-related behavioral changes were investigated by elevated plus-maze and hole-board systems. In the elevated plus-maze, exposed rats spent proportionally less time on open arms than control rats (0.12±0.08 versus 0.34±0.18, p <0.01), while hole-board results showed no statistically significant differences between the two groups.
Korpinar MA, Kalkan MT, Tuncel H. · 2012
Researchers exposed rats to 50 Hz magnetic fields (the same frequency as household electrical wiring) for 21 days and measured their anxiety levels using standard behavioral tests. The exposed rats showed significantly higher anxiety and stress-related behaviors compared to unexposed rats, spending much less time in open, exposed areas of test mazes. This suggests that prolonged exposure to power-frequency magnetic fields may increase stress responses in the brain.
Henrykowska G et al. · 2009
Researchers exposed human blood platelet suspensions for 15 minutes to a 50 Hz magnetic field of different shapes at a flux density of 10 mT. The study measured catalase and superoxide dismutase activity, malondialdehyde concentration, and free radical generation in human blood platelets. Free radicals, malondialdehyde, and catalase all showed increased values compared with the initial level regardless of field shape, while superoxide dismutase activity was lower than at the onset of the experiment.
Sahebjamei H, Abdolmaleki P, Ghanati F · 2007
Researchers investigated the effects of static magnetic fields on antioxidant enzyme activities in suspension-cultured tobacco cells. Cells were exposed to static magnetic fields at 10 and 30 mT for 5 days, 5 hours each day, and compared with control cells. Superoxide dismutase activity increased with exposure, while catalase and ascorbate peroxidase activity decreased, and lipid peroxidation levels also increased.
J. A. Martin · 1970
This 1970 technical report examined the biological effects of electromagnetic fields from what's called a 'siege array,' focusing on microwave radiation's impact on the human body. The research investigated pulse field effects and radiation heating patterns, representing early military or defense-related EMF exposure studies. This work contributed to understanding how concentrated electromagnetic energy affects human biology.
Wang X et al. · 2008
Researchers exposed rats to extremely low-frequency electromagnetic fields (20 Hz, 14 mT) for one hour per day before daily morphine injections for 12 days, then measured dopamine D2 receptor density in the dorsal hippocampus during withdrawal. Morphine withdrawal alone significantly lowered D2 receptor density compared to saline controls, and ELF-EMF exposure during morphine treatment further reduced receptor density on the first and third withdrawal days. In both groups, D2 receptor density tended to recover as the number of withdrawal days increased.
Jouni FJ, Abdolmaleki P, Ghanati F. · 2012
Researchers studied whether a static magnetic field affects oxidative stress in Vicia faba plants grown in soil from a high background natural radioactivity area of Iran. Plants were continuously exposed to a static magnetic field of 15 mT for 8 days, each 8 hours per day. Antioxidant enzyme activity and flavonoid content were lower in plants from both high and low natural radioactivity soils compared to the control. The results suggest that static magnetic field exposure causes accumulation of reactive oxygen species, and that natural radioactivity of soil exaggerates oxidative stress.
Ahmed Z, Wieraszko A. · 2008
Researchers exposed brain tissue from the hippocampus (memory center) to pulsed magnetic fields for 30 minutes. The neurons became significantly more electrically active, firing more signals and changing how they communicate. This shows magnetic fields can directly alter brain cell function.
Ahmed Z, Wieraszko A. · 2008
Researchers investigated the influence of a pulsed magnetic field on hippocampal evoked potentials in vitro. Exposure to a pulsed magnetic field at 0.16 Hz and 15 mT, applied for 30 minutes, amplified the population spike and the slope of EPSP recorded from hippocampal tissue. The amplification was additive to previously induced long-term potentiation and occurred in an NMDA-independent way. The pulsed magnetic field also modified paired-pulse facilitation and paired-pulse inhibition, suggesting it affects both excitatory and inhibitory processes in the hippocampus.
Shokrollahi S, Ghanati F, Sajedi RH, Sharifi M · 2018
Researchers exposed soybean plants to static magnetic fields of 0, 20, and 30 mT for 5 days at 5 hours each day, examining ferrous content and the gene expression and activity of ferritin and catalase. At 20 mT, gene expression of the iron transporter, ferrous content, hydrogen peroxide content, and ferritin and catalase gene expression, content, and activity all decreased. Opposite responses were observed under 30 mT treatments. The researchers also evaluated purified ferritin and apoferritin from horse spleen and catalase from bovine liver under the static magnetic field, finding that tertiary structure, size, and activity were altered, while secondary structure was not influenced.
Vignola MB et al. · 2012
Researchers evaluated the effect of pulsed electromagnetic field (PEMF) therapy on inflammatory biomarkers associated with oxidative stress in rats with experimental myopathy. Myopathy was induced by injecting 50 μl of 1% carrageenan, and PEMF treatment was applied at 20 mT and 50 Hz for 30 min/day/rat. PEMF treatment was associated with decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery.
G. AKOYUNOGLOU · 1964
Khadir R, Morgan JL, Murray JJ. · 1999
Researchers investigated the effects of a sinusoidal 60 Hz magnetic field on free radical production, degranulation, and viability in human neutrophils. Exposure of unstimulated human neutrophils to the magnetic field did not affect the functions examined. When cells were also stimulated with PMA, exposure to a 22 mT, 60 Hz magnetic field induced significant increases in superoxide anion production of 26.5% and in β-glucuronidase release of 53%. Release of lysozyme and lactate dehydrogenase was unchanged by the magnetic field, whether the cells were stimulated or not.
Frilot C 2nd, Carrubba S, Marino AA. · 2011
Researchers exposed rats to a 2.5-G, 60-Hz magnetic field and used positron emission tomography with fluorodeoxyglucose to identify brain regions where glucose uptake changed. Increased glucose utilization occurred in hindbrain voxels when the field was applied orthogonally to the sagittal plane, but not when the angle between the field and the sagittal plane varied randomly. Distinct activation effects were observed in response to transient and steady-state magnetic stimuli.
Rajabbeigi E, Ghanati F, Abdolmaleki P, Payez A · 2013
Researchers studied how parsley cells responded to 21 ppm iron and a static magnetic field (SMF; 30 mT), measuring antioxidant enzymes and related molecules at 6 and 12 hours after treatments. SMF exposure alone increased catalase activity, but when combined with iron, or with iron alone, catalase activity decreased compared to control cells. The combination of SMF with iron reduced the iron content of cells and lessened iron's negative effect on catalase activity. Ferritin levels dropped significantly in SMF-treated and SMF-plus-iron-treated cells at 12 hours, leading the researchers to question ferritin's proposed role as a reactive oxygen species detoxifying molecule.
Payez A et al. · 2013
Both field types increased the speed of germination but did not affect germination percent, compared to the control group. EMF significantly reduced seedling length and vigor index I, while SMF had no effects on these parameters, but both treatments significantly increased vigor index II. Both treatments increased catalase activity and proline contents but reduced peroxidase activity, lipid peroxidation, and electrolyte leakages of membranes.
Sullivan K, Balin AK, Allen RG · 2011
Researchers determined the effects of a static magnetic field (SMF) on the proliferation of various types of human cells. SMF significantly decreased the initial attachment of fibroblasts and their subsequent growth, with effects observed in both fetal lung (WI-38) and adult skin fibroblast lines. SMF inhibited melanoma cell growth by 20% on day 7, but did not affect melanoma cell attachment. Oxidant production increased 37% in WI-38 cells exposed to SMF during the first 18 hours after seeding, but no elevation in oxidant levels was observed after a prolonged 5-day exposure.
Todorović D et al. · 2013
Researchers exposed beetles to a strong static magnetic field (1,000 times Earth's strength) and found it didn't affect development time but did alter movement patterns in one species, with effects varying by magnetic pole orientation, showing static fields can influence animal behavior.
