Unknown authors · 2005
Researchers exposed cultured cells from different tissues to intermittent 50 Hz, 1 mT ELF-EMF for 1-24 hours. They used alkaline and neutral comet assays to assess ELF-EMF-induced DNA strand breaks. The study identified three responder cell types (human fibroblasts, human melanocytes, rat granulosa cells) and three non-responder cell types (human lymphocytes, human monocytes, human skeletal muscle cells).
Unknown authors · 2005
Researchers exposed six different types of human and animal cells to power line frequency electromagnetic fields (50 Hz, 1 milliTesla) for up to 24 hours. They found that three cell types showed DNA damage while three others remained unaffected, demonstrating that EMF sensitivity varies significantly between different tissues and cell types.
Tkalec M, Malaric K, Pevalek-Kozlina B. · 2005
Researchers evaluated duckweed growth and peroxidase activity after exposure in a Gigahertz Transversal Electromagnetic (GTEM) cell to electric fields at frequencies of 400, 900, and 1900 MHz. Plants exposed for 2 h to a 23 V/m field at 900 MHz showed significantly decreased growth compared with the control, while the same field strength at 400 MHz did not have such an effect. Peroxidase activity in exposed plants varied depending on exposure characteristics, with a significant increase of 41% found in plants exposed for 2 h to 41 V/m at 900 MHz.
Lee S et al. · 2005
The study used the serial analysis of gene expression (SAGE) method to measure genome-wide gene expression changes in cultured human HL-60 cells exposed to pulsed 2.45 GHz RF fields. After a 2-hour exposure, 221 genes showed altered expression, increasing to 759 genes after 6 hours. Apoptosis-related genes were upregulated while cell cycle genes were downregulated, with no significant increase in heat shock genes.
Lee S et al. · 2005
Researchers exposed human blood cells to 2.45 GHz radiofrequency radiation (the same frequency used in WiFi and microwave ovens) and found it altered the activity of hundreds of genes. After just 2 hours, 221 genes changed their expression, increasing to 759 genes after 6 hours. The affected genes were involved in cell death and cell division processes, suggesting biological effects occur through non-heat mechanisms.
Unknown authors · 2005
Researchers exposed cultured human fibroblasts to intermittent 50 Hz ELF-EMF (5 minutes on, 10 minutes off) at 1 mT for 2 to 24 hours. Micronuclei frequency increased in a time-dependent manner, becoming statistically significant after 10 hours and plateauing at roughly three times the basal level after about 15 hours. Chromosomal aberrations were also elevated, reaching up to 10-fold above baseline.
Unknown authors · 2005
Researchers exposed human skin cells to 50 Hz electromagnetic fields (like power lines) in an on-off pattern and found significant chromosomal damage. The cells showed three times more micronuclei (broken chromosome fragments) and up to 10 times more chromosomal breaks after 10-15 hours of exposure. This suggests power-frequency EMFs can damage DNA in human cells.
Miyakoshi J et al. · 2005
Researchers exposed human glioma MO54 cells to a continuous wave 1950 MHz RF field at SARs of 1, 2, and 10 W/kg for up to 2 hours. Cell growth was assessed by counting cell numbers up to 4 days after exposure. RF exposure at 2 and 10 W/kg did not affect the growth of MO54 cells. However, exposure at 10 W/kg for 1 and 2 hours significantly decreased the protein level of phosphorylated Hsp27 at serine 78.
Harakawa S et al. · 2005
The effects of exposure to extremely low frequency electric fields (ELF EFs) on plasma lipid peroxide levels and antioxidant activity (AOA) in Sprague–Dawley rats were studied. The test compared rats treated with a combination of the oxidizing agent AAPH and a 50 Hz EF of 17.5 kV/m for 15 min per day for 7 days, as well as AAPH alone, EF alone, or no treatment. EF significantly decreased the plasma peroxide level in rats treated with AAPH, similar to treatment by ascorbic acid or the superoxide dismutase.
Keshvari J, Lang S. · 2005
Researchers used the finite-difference time-domain (FDTD) computational method to calculate specific absorption rate (SAR) in anatomically correct adult and child head models exposed to a half-wave dipole at 900, 1800, and 2450 MHz. The ear and eye regions were studied because they represent realistic exposure scenarios for mobile wireless communication devices.
Cosquer B, Galani R, Kuster N, Cassel JC. · 2005
In a first phase, researchers validated the elevated plus-maze apparatus in male Sprague-Dawley rats. They tested anxiety responses at light intensities of 200, 30, 10, and 2.5 lux and with diazepam doses of 0.5 and 1.0 mg/kg. Anxiety responses decreased with decreasing light intensity and were attenuated by diazepam. A separate group of rats was then exposed to 2.45 GHz EMFs with a whole-body SAR of 0.6 W/kg and brain-averaged SAR of 0.9 W/kg for 45 min. EMF exposure did not significantly affect anxiety responses at either low or high ambient light intensity.
Lai H, Singh NP · 2005
Researchers studied four groups of rats: one exposed to 2450-MHz microwaves at 1 mW/cm2, one to magnetic field noise at 45 mG, one to both simultaneously, and a sham-exposed group. Brain cells of microwave-exposed rats had significantly higher levels of DNA single- and double-strand breaks compared with sham-exposed animals. Noise exposure alone did not significantly affect DNA strand break levels, but simultaneous noise exposure blocked the microwave-induced increases.
Lai H, Singh NP · 2005
Researchers exposed rats to cell phone-frequency microwaves (2450 MHz) for 2 hours and found significant DNA damage in brain cells. However, when they simultaneously exposed the rats to a weak magnetic field with random fluctuations, it completely blocked the DNA damage from occurring. This suggests that certain types of magnetic field exposure might actually protect against some forms of EMF damage.
Unknown authors · 2005
Researchers exposed two clones of MCF-7 breast cancer cells to 50Hz electromagnetic fields and calculated IC(50) values from tamoxifen dose-response curves. The dose-response curves shifted to higher tamoxifen concentrations in an intensity-dependent manner, with a maximal response at 1.2muT. The authors hypothesized that electromagnetic field exposure could contribute to tamoxifen resistance observed in breast cancer after long-term treatment.
Unknown authors · 2005
German researchers exposed breast cancer cells to 50 Hz electromagnetic fields (the same frequency as power lines) and found that EMF exposure made the cells more resistant to tamoxifen, a common breast cancer drug. The effect was strongest at 1.2 microTesla field strength, suggesting that power-frequency EMF exposure could interfere with cancer treatment effectiveness.
Schmid G, Sauter C, Stepansky R, Lobentanz IS, Zeitlhofer J · 2005
Researchers exposed 58 healthy volunteers to a generic 1.97 GHz UMTS-like signal in a double-blinded crossover design. Each subject completed four clinical visual perception tests under high exposure, low exposure, and sham conditions. High exposure produced an average SAR of 0.63 W/kg (1 g) and 0.37 W/kg (10 g) in the left temporal cortex. No statistically significant differences in visual perception parameters were found between any exposure condition and sham.
Unknown authors · 2005
Residential exposure was calculated as time-weighted average magnetic fields from power lines from 1967 to diagnosis, while occupational exposure was assessed via job titles classified by hours per week above 0.1 μT from 1955 to diagnosis. The two highest residential exposure categories showed elevated odds ratios for all brain tumours (OR = 1.6, 95% CI 0.9–2.7 and OR = 1.3, 95% CI 0.7–2.3), but the risk was not statistically significant and no clear exposure-response pattern emerged. Occupational exposure showed no association with brain tumours for any site, and the occupational exposure groups showed an inverse association.
Komatsubara Y et al. · 2005
Researchers exposed murine m5S cells to 2.45 GHz high-frequency electromagnetic fields for 2 hours at SARs of 5, 10, 20, 50, and 100 W/kg continuous wave and at a mean SAR of 100 W/kg pulse wave with a maximum of 900 W/kg. The effects were compared with sham-exposed controls and with mitomycin C and X-ray treatments as positive controls. No significant differences in chromosomal aberrations were observed between HFEMF-exposed cells and sham controls, while positive controls increased chromatid-type and chromosome-type aberrations.
Unknown authors · 2005
Researchers exposed 24 male Wistar rats to 50 Hz electromagnetic fields at 100-300 microT for 4 hours daily over one month. Skin and thyroid samples were analyzed using immunohistochemistry and stereology with markers for serotonin, substance P, CGRP, PGP 9.5, and NPY. Exposed rats had significantly more serotonin-positive mast cells in skin and NPY-containing nerve fibers in thyroid compared to controls.
Unknown authors · 2005
Researchers investigated whether acute magnetic-field exposure could elicit DNA damage in brain cells from adult rats, adult mice, and immature mice. Rodents were exposed to a 60 Hz magnetic field at 0, 0.1, 1, or 2 mT for 2 h and brain cells were analyzed at 0, 2, and 4 h after exposure using the alkaline comet assay. No significant increase in DNA damage was detected at any magnetic-field strength or time point.
Martinez-Burdalo M, Martin A, Anguiano M, Villar R · 2005
Researchers analyzed safety assessment procedures for human exposure near cellular base-station antennas at 900, 1800, and 2170 MHz. They compared distances where electromagnetic fields were below reference levels with distances where specific absorption rate (SAR) values in an exposed person were below basic restrictions according to European guidelines. A high-resolution human body model was placed in front of each antenna at different distances to compute whole-body averaged SAR and maximum 10 g averaged SAR. The finite-difference time-domain method was used for both electromagnetic field and SAR calculations.
Cosquer B, Vasconcelos AP, Frohlich J, Cassel JC. · 2005
Wang J et al. · 2005
Researchers exposed mouse C3H10T1/2 cells to 2450 MHz HFEMF at SARs of 5 to 200 W/kg for 2 hours, with or without methylcholanthrene (MC) and the tumor promoter TPA. Transformed foci of Type II and Type III were counted as the endpoint. No significant differences were observed in malignant transformation frequency between controls and HFEMF with or without TPA. Transformation frequency increased for HFEMF at SAR above 100 W/kg when combined with MC or MC plus TPA, while heat exposure did not increase transformation.
Unknown authors · 2005
Researchers exposed two-month-old Wistar male rats to 50 Hz electromagnetic fields at 100-300 microT and 54-160 V/m for 4 h a day, seven days a week for one month. After sacrifice, skin and thyroid samples were processed for toluidine blue staining or indirect immunohistochemistry, and stereological analysis was performed using an M42 grid and a special microscopic frame. The numerical and volume density of intact type A mast cells in the thyroid was significantly higher in the exposed group compared to controls. Mast cells and immunoreactive nerve fibers were observed in the skin, and histamine-immunoreactive mast cells were observed in the thyroid of exposed animals. However, the differences in stereological data were not statistically significant by the Mann-Whitney test.
Unknown authors · 2005
Serbian researchers exposed male rats to 50 Hz electromagnetic fields (the same frequency as power lines) for 4 hours daily over one month. They found significant increases in specific immune cells called mast cells in the thyroid gland, along with changes to nerve fibers in skin tissue. These cellular changes suggest the body's immune and nervous systems respond to power-frequency EMF exposure.
