Unknown authors · 1996
Researchers tested whether 60 Hz electromagnetic fields (like those from power lines) could stimulate breast cancer cell growth or cause DNA damage in laboratory conditions. The study found that while estrogen and chemical estrogens promoted cancer cell division, electromagnetic field exposure at multiple intensities showed no effect on cell growth, gene activity, or DNA damage.
Detlavs I et al. · 1996
Researchers exposed Wistar rats with full-thickness dermal wounds to RF EMF for 30 min daily during the first 5 days using 53.53 GHz without modulation, 42.19 GHz without modulation, and 42.19 GHz with a 200-MHz frequency modulation band. RF EMF without frequency modulation decreased glycoprotein macromolecules and reduced inflammatory exudation and total collagen accumulation. RF EMF with frequency modulation significantly elevated hexoses and sialic acid concentrations, indicating intensified exudative phenomena, and increased collagenous protein accumulation. The authors concluded that non-thermal EMF affects reparative-proliferative processes in soft tissue wounds.
Unknown authors · 1996
Researchers treated cultured mammalian cells with three carcinogens that produce DNA single-strand breaks. The repair rate of these lesions was unchanged by 50 Hz electric, magnetic, or combined fields across a range of intensities.
Elekes E, Thuróczy G, Szabó LD. · 1996
Researchers tested the effect of continuous or amplitude-modulated 2.45 GHz microwave radiation on the immune response of mice. CW exposures induced elevations of the number of antibody-producing cells in the spleen of male Balb/c mice by 37%. AM microwave exposure induced elevation of the spleen index by 15% and antibody-producing cell number by 55% in the spleen of male mice. No changes were observed in female mice.
Elekes, E, Thuroczy, G, Szabo, LD · 1996
Researchers exposed male and female mice to microwave radiation at 2.45 GHz (similar to microwave ovens and WiFi) for 3 hours daily over 6 days to test effects on immune function. They found that both continuous and pulsed microwave exposure significantly increased antibody production in male mice (37-55% increases), but had no effect on female mice. This suggests that microwave radiation can stimulate immune system activity, with males appearing more sensitive than females.
Kubinyi G, Thuroczy G, Bakos J, Boloni E, Sinay H, Szabo LD, · 1996
Pregnant CFLP mice were exposed to 2.45 GHz microwave radiation for 100 minutes each day throughout gestation in an anechoic chamber. Brain aminoacyl-tRNA synthetase activity decreased significantly after continuous-wave exposure but not after amplitude-modulated exposure. Liver enzyme activity increased after both continuous-wave and amplitude-modulated exposure, while body and organ weights were unaffected.
Urech, M, Eicher, B, Siegenthaler, J · 1996
Researchers conducted field experiments lasting one to three years alongside laboratory tests, exposing Parmelia tiliacea and Hypogymnia physodes to 2.45 GHz microwaves at 0.2, 5, and 50 mW/cm² with a control. Both species showed a substantially reduced growth rate at the highest exposure of 50 mW/cm². Temperature measurements revealed that microwaves at 2.45 GHz and 50 mW/cm² substantially increased lichen surface temperature and accelerated drying. When the same species were exposed near a 9.5 MHz short-wave broadcast transmitter at up to 235 V/m, no visible effects were detected.
Unknown authors · 1996
Researchers investigated interactions of weak ELFMFs with primitive haemopoietic cells in vitro using the multipotential progenitor cell line FDCP-mix(A4). They determined proliferative activity and clonogenic potential under optimal and sub-optimal growth conditions with exposures to ambient laboratory ELFMFs or three other ELFMF regimes including nulled fields, Ca2+-ion cyclotron resonance at 50 Hz, and vertical 50 Hz fields of 6 muT(RMS). Using exposures of 1, 4, 7 and 21 days, they found no significant alteration of growth rate, cell-cycle state or clonogenic efficiency.
Unknown authors · 1996
Researchers exposed blood stem cells to extremely low frequency magnetic fields similar to those from power lines for up to 21 days. The fields had no effect on cell growth, division, or ability to form colonies. This laboratory study found no evidence that power line EMF directly promotes blood cancer development in these primitive blood cells.
Singh B, Bate LA · 1996
Researchers exposed pigs to infrared, low-power (6.1 mW cm-2), and high-power (11.4 mW cm-2) 915 MHz microwave radiation for 24 hours, with controls under natural light. Lung tissues were examined ultrastructurally and for acid phosphatase activity, and rough endoplasmic reticulum fraction was measured. Low-power microwave exposure increased the rough endoplasmic reticulum to cytoplasm ratio, prominent Golgi profiles, and secretory vesicles in pulmonary intravascular macrophages compared to other groups. High-power microwave exposure caused extracellular acid phosphatase precipitates and disrupted collagen matrix in the pulmonary interstitium. Intracellular globules appeared in infrared and low-power microwave groups but not in controls or high-power group.
Pashovskina MS, Akoev IG · 1996
A solution of rat muscle actomyosin was exposed to 2375 MHz pulse-modulated microwave radiation with rectangular pulse modulation ranging from 50 to 300 pulses per second. The study found that actomyosin ATPase activity was dependent on both modulation frequency and microwave intensity. Specific modulation frequencies were found to change the ATPase activity of actomyosin.
Sparks PB, Mond HG, Joyner KH, Wood MP · 1996
Researchers exposed 16 implanted Telectronics META pulse generators to simulated GSM transmissions and a 2-W digital mobile telephone. The devices were programmed to maximum sensitivity and tested in unipolar and bipolar settings and rate adaptive and nonrate adaptive modes. At maximum sensitivity, 11 of 16 devices showed no EMI, while brief ventricular triggering, pauses, and one episode of pacemaker-mediated tachycardia occurred in the remaining devices. When programmed to routine sensitivities, only one device displayed rare single beat ventricular triggering, and no changes in minute ventilation rate adaptive pacing were observed.
Lai H, Singh NP · 1995
Researchers measured DNA single-strand breaks in brain cells of rats acutely exposed to low-intensity 2450 MHz microwaves, using an alkaline microgel electrophoresis method. After 2 hours of exposure to pulsed microwaves, no significant effect was observed immediately after exposure, but a dose rate-dependent increase in DNA single-strand breaks was found in brain cells at 4 hours postexposure. In rats exposed for 2 hours to continuous-wave 2450 MHz microwaves at 1.2 W/kg SAR, increases in brain cell DNA single-strand breaks were observed both immediately and at 4 hours postexposure.
Lai H, Singh NP, · 1995
Researchers exposed rats to microwave radiation at levels similar to cell phone use and found that it caused DNA breaks in brain cells. The damage appeared 4 hours after exposure, even at relatively low power levels (0.6 W/kg). This suggests that microwave radiation can damage the genetic material in brain cells at exposure levels considered 'safe' by current standards.
Roy S et al. · 1995
Researchers investigated whether a low-frequency, low-intensity magnetic field could affect the oxidative burst triggered by PMA in rat peritoneal neutrophils. In the presence of the magnetic field, a 12.4% increase in the fluorescence signal was observed in PMA-stimulated neutrophils (n = 5, P < 0.02, 18 pairs of measurements). The researchers described this as what they believed to be the first experimental observation of a magnetic field influencing free radical species generated during signal transduction in living cells.
Unknown authors · 1995
The study used quantitative Northern analysis to measure MYC and beta-actin mRNA levels in human leukemic HL60 cells. Cells were exposed to 60 Hz sinusoidal magnetic fields at 0.57, 5.7, or 57 microT for 20 minutes using a new field-exposure system designed to optimize any response. No significant effect of the electromagnetic fields on gene expression was observed with any of the exposure systems or analytical methods.
Unknown authors · 1995
Researchers exposed human leukemia cells to 60 Hz magnetic fields at various strengths for 20 minutes to test whether power line frequency EMF could activate cancer-related genes. Despite using improved methods and testing conditions similar to previous positive studies, they found no effect on MYC or beta-actin gene expression. This contradicts earlier claims that EMF exposure rapidly activates genes involved in cell growth.
Lacy-Hulbert et al. · 1995
Researchers exposed human leukemia cells (HL60) to 60 Hz magnetic fields at various strengths for 20 minutes, then measured whether genes linked to cancer growth (MYC and beta-actin) became more active. Despite using conditions similar to previous studies that claimed to find effects, they found no changes in gene activity from the electromagnetic field exposure.
Unknown authors · 1995
Researchers performed an extensive series of experiments with human HL60 and Daudi cells incorporating blind exposures and internal standards. Exposure conditions covered flux densities from 5.7 microT to 10 mT and durations from 20 to 60 minutes. No alteration in the human MYC gene (c-myc) or beta-actin steady-state mRNA levels was observed.
Kakita Y et al. · 1995
Researchers studied the effect of microwave irradiation on the survival of bacteriophage PL-1 specific for Lactobacillus casei using a commercial 2,450 MHz microwave oven. Phage inactivation followed almost first-order reaction kinetics and was unaffected by continuous versus intermittent irradiation or phage concentration but was affected by suspension volume which prevented heat loss. Phage genome DNA breakage was primarily caused by heat generated by microwave irradiation, while the same temperature from external heat did not affect phage DNA.
Chou CK, McDougall JA, Can KW · 1995
Researchers tested the possibility of tissue heating from an auditory brainstem implant and a modified cochlear implant during MRI using a full-sized human phantom with simulated skull, brain, and muscle. Thermograms were taken before and after a 26-minute MRI scan with 94 excitations and 25 ms echo time set to induce maximum radiofrequency heating. Results showed no observable heating associated with either implant during worst-case MRI of the head.
Sherry CJ, Blick DW, Walters TJ, Brown GC, Murphy MR · 1995
Researchers examined the effect of acute ultrawideband electromagnetic radiation exposure on monkeys performing a Primate Equilibrium Platform task. The exposure had no effect on task performance when tested immediately after exposure. Each monkey was exposed twice for 2 minutes at a pulse repetition rate of 60 Hz with a peak electric field strength of 250 kV/m.
Unknown authors · 1995
Researchers exposed human lymphoid cells immortalized by Epstein-Barr virus and HL60 leukemic cells to 50 Hz magnetic fields at 10 μT or 1 mT for periods ranging from 20 minutes to 72 hours. C-myc transcript levels were measured by Northern blot analysis normalized to the GaPDH gene. No statistically significant difference in c-myc transcript levels was observed between exposed and control cells for either cell type at any exposure duration, and the same result was found in cells synchronized in G0/G1 phase.
Pakhomov AG, Dubovick BV, Degtyariov IG, Pronkevich AN · 1995
Pakhomov AG, Dubovick BV, Degtyariov IG, Pronkevich AN · 1995
Researchers studied the combined effects of microwave radiation and drugs on an isolated frog auricle preparation. Exposure to pulse-modulated 915 MHz microwaves for up to 40 minutes had no effect on the rate or amplitude of spontaneous auricle twitches unless the absorbed power caused heating. Caffeine (1 mM) strongly increased the average heart power, and this effect was significantly augmented by about 15% under burst-type pulsed microwaves with an average SAR of 8-10 W/kg, while heating was only approximately 0.1°C.
