Sarapultseva EI, Igolkina JV, Tikhonov VN, Dubrova YE · 2014
Researchers exposed the ciliate Spirostomum ambiguum to 1 GHz and 10 GHz radiofrequency electromagnetic fields at power flux densities ranging from 0.05 to 0.5 W/m2 for periods from 0.05 to 10 hours. Exposure to 0.1 W/m2 of either 1 or 10 GHz radiofrequency fields resulted in a significant decrease in the motility of the ciliates. The motility of unexposed offspring of ciliates that had been irradiated with 0.1 W/m2 of 10 GHz radiofrequency fields remained significantly compromised across at least 10 to 15 generations.
Unknown authors · 2013
Researchers exposed male CD1 mice to 50 Hz ELF magnetic fields at 0.1, 0.2, 1, or 2 mT for 7 days, 15 hours per day. Hsp70 expression was measured in cerebral cortex-striatum, hippocampus, and cerebellum using RT-PCR and Western blot. DNA damage was assessed in the same tissues by comet assay. No changes in hsp70 mRNA or protein occurred except a weak mRNA increase in hippocampus at 0.1 mT. Mice exposed to 1 or 2 mT and sacrificed immediately showed increased DNA strand breaks in all brain areas, which returned to baseline after 24 hours.
Unknown authors · 2013
Researchers exposed peripheral blood samples from healthy non-smoking male donors to 3T MRI electromagnetic fields for durations of 0, 22, 45, 67, and 89 minutes, with a positive control exposed to 4 Gy of cobalt-60 gamma radiation. DNA single-strand breaks were measured by the comet assay, and chromosome breakage, loss, and micronuclei formation were measured by the micronucleus test. The comet assay and micronucleus test showed no DNA damage from MRI exposure at any duration.
Unknown authors · 2013
Unknown authors · 2013
Researchers examined the effects of mobile phone radiation on developing neural tissue of chick embryos using four study groups. TUNEL results were statistically significant at 30 and 48 hours in the third group. Caspase-3 was negative in the control group at 48 and 72 hours and showed moderate activity in the third group. The study concluded that mobile phone radiation caused developmental delay in early chick embryos.
Unknown authors · 2013
Rago R et al. · 2013
The study enrolled healthy men and divided them into four groups based on daily mobile phone use: no use (10 men), less than two hours (16 men), two to four hours (17 men), and more than four hours (20 men). Among the men using phones more than four hours daily, researchers compared 12 who carried the phone in a trousers pocket with 8 who carried it in a shirt pocket. Conventional sperm parameters such as density, total count, morphology, and progressive motility did not differ significantly between groups. However, sperm DNA fragmentation was higher in the group using phones more than four hours per day and in those who carried the phone in a trousers pocket.
Gurbuz N, Sirav B, Colbay M, Yetkin I, Seyhan N. · 2013
Researchers exposed male Wistar albino rats to 1800 MHz or 2100 MHz radiofrequency radiation for 30 min per day, 6 days per week, for either a month or two months. They measured micronucleus frequency in exfoliated bladder cells as an indicator of genotoxic damage. No significant differences in micronucleus frequency were found between any exposed group and its corresponding control group, nor between the two 2100 MHz exposure durations.
Waldmann P et al. · 2013
Researchers investigated whether GSM 1,800 MHz radiofrequency fields cause genotoxic effects in human lymphocytes. Blood from 20 healthy volunteers was exposed to three specific absorption rates of 0.2, 2, and 10 W/kg and sham for 28 hours. Four genotoxicity tests were conducted, including chromosome aberration, micronucleus, sister chromatid exchange, and comet assays. None of the nine endpoints showed a significant and reproducible exposure effect across the three analyzing laboratories. Positive controls showed highly significant differences from sham exposures, validating the study methods.
Pesnya DS, Romanovsky AV. · 2013
Liu C et al. · 2013
Li SS, Zhang ZY, Yang CJ, Lian HY, Cai P · 2013
Researchers studied the effects of short-term and long-term ELF-EMF exposure on male Drosophila melanogaster using transcriptomic analysis and QRT-PCR. Transcriptomic analysis showed that short-term exposure up-regulated 439 genes and down-regulated 874 genes, while long-term exposure up-regulated 514 genes and down-regulated 1206 genes. Short-term exposure may decrease male reproductive ability, but long-term exposure had no effect. QRT-PCR confirmed that jra, ark, and decay genes were down-regulated after exposure for one generation and 72 hours, suggesting apoptosis may be inhibited.
Zhijian C et al. · 2013
The study used protein microarray to examine protein expression in human B lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation at a specific absorption rate of 2.0 W/kg for 24 hours. The analysis identified 27 differentially expressed proteins associated with DNA damage repair, apoptosis, oncogenesis, cell cycle, and proliferation, with a fold change greater than 1.5 and P<0.05. Western blot validation confirmed that RPA32 expression was significantly down-regulated and p73 expression was significantly up-regulated in the exposed group (P<0.05).
Xu S et al. · 2013
Tsybulin O et al. · 2013
Japanese quail embryos were exposed in ovo to GSM 900 MHz radiation for 38 hours or 158 hours with a 48-second on, 12-second off cycle at an average power density of 0.25 μW/cm(2) and a specific absorption rate of 3 μW/kg. The number of differentiated somites was assessed microscopically and DNA damage was assessed by an alkaline comet assay. After 38 hours the number of differentiated somites increased and DNA strand breaks decreased, while after 158 hours the number of differentiated somites decreased and DNA damage increased.
Jiang B, Zong C, Zhao H, Ji Y, Tong J, Cao Y · 2013
Adult male ICR mice were pre-exposed to 900 MHz radiofrequency fields for 4 hours per day for 7 days, then given an acute whole-body dose of 3 Gy gamma radiation. The micronucleus assay was used to measure genotoxicity in immature erythrocytes in peripheral blood and bone marrow. Mice exposed to the radiofrequency fields alone showed micronucleus indices similar to unexposed controls, while mice given gamma radiation alone showed a significantly increased micronucleus frequency. Mice pre-exposed to the radiofrequency fields before gamma radiation showed a significant decrease in micronucleus indices compared to those given gamma radiation alone.
Atlı Şekeroğlu Z, Akar A, Sekeroğlu V. · 2013
Researchers investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields on bone marrow cells of immature and mature rats. Rats in the treatment groups were exposed to RF-EMF for 2 hours per day for 45 days. Significant differences were observed in chromosome aberrations, micronucleus frequency, mitotic index, and ratio of polychromatic erythrocytes in all treatment and recovery groups. A 15-day recovery period after exposure did not reduce the damage to the same extent as the corresponding control groups.
Tkalec M, Stambuk A, Srut M, Malarić K, Klobučar GI. · 2013
Researchers exposed earthworms (Eisenia fetida) in vivo to 900 MHz radiofrequency electromagnetic fields at field levels of 10, 23, 41 and 120 V m(-1) for 2 hours using a Gigahertz Transversal Electromagnetic (GTEM) cell. All exposure treatments induced significant genotoxic effects in earthworm coelomocytes, as detected by the Comet assay. The results also indicated enhanced catalase and glutathione reductase activity, as well as lipid and protein oxidative damage. Antioxidant responses and the potential for damage to lipids, proteins, and DNA differed depending on field level, field modulation, and duration of exposure.
Shahin S et al. · 2013
Twelve-week-old female mice were exposed to 2.45 GHz microwave radiation for 2 hours per day for 45 days to study effects on implantation and pregnancy. Implantation sites were significantly affected in the microwave-irradiated mice compared to controls. The researchers concluded that low-level microwave irradiation-induced oxidative stress suppresses implantation and may also lead to deformity of the embryo if pregnancy continues.
Liu C, Duan W, Xu S, Chen C, He M, Zhang L, Yu Z, Zhou Z. · 2013
Researchers exposed a mouse spermatocyte-derived GC-2 cell line to 1800 MHz GSM signals for 24 hours using an intermittent schedule of 5 minutes on and 10 minutes off, at SAR levels of 1 W/kg, 2 W/kg, or 4 W/kg. At a SAR of 4 W/kg, DNA migration in a modified comet assay was significantly increased, and levels of the DNA adduct 8-oxoguanine (8-oxoG) were also increased. No detectable DNA strand breakage was observed by the alkaline comet assay. The increases in DNA damage were accompanied by increases in reactive oxygen species (ROS), and these effects were mitigated by co-treatment with the antioxidant alpha-tocopherol.
Hancı H et al. · 2013
Researchers exposed pregnant rats to 900-MHz EMF during days 13–21 of pregnancy and examined the testicles of their offspring at postnatal day 21. The offspring of exposed mothers showed irregularities in seminiferous tubule basal membrane and epithelium, immature germ cells in the lumen, and a decreased diameter in seminiferous tubules and thickness of epithelium. Apoptotic index, lipid peroxidation, and DNA oxidation were higher in the offspring of exposed mothers than in controls.
Burlaka A et al. · 2013
Researchers exposed Japanese quail embryos in ovo to GSM 900 MHz radiofrequency radiation at 0.25 µW/cm2 for 158 to 360 hours on a discontinuous schedule. The exposure produced a significant persistent overproduction of superoxide and nitrogen oxide in embryo cells throughout the analysis period. Exposed embryo cells also showed significantly increased levels of TBARS and 8-oxo-dG alongside significantly decreased superoxide dismutase and catalase activities.
Atasoy HI, Gunal MY, Atasoy P, Elgun S, Bugdayci G · 2013
Researchers exposed male rats to radiofrequency radiation from Wi-Fi devices operating at 2.437 GHz for 24 hours a day for 20 weeks, then examined their testes. The exposed rats showed significant increases in markers indicating DNA damage in the testes compared to controls. Catalase and glutathione peroxidase activity were decreased in the exposed group, which the researchers suggested may have been due to radiofrequency effects on enzyme activity. The researchers said these findings raise questions about the safety of radiofrequency exposure from Wi-Fi Internet access devices for growing organisms of reproductive age, with a potential effect on both fertility and the integrity of germ cells.
Calabrò E et al. · 2013
Researchers used SH-SY5Y neuroblastoma cells as an experimental model to study the effects of a 50 Hz electromagnetic field in the range from 50 µT to 1.4 mT. Fourier transform infrared spectroscopy analysis showed a reduction in intensity of the amide A band and a slight increase of vibration bands corresponding to methylene groups. Exposure to a 50 Hz electromagnetic field higher than 0.8 mT was associated with a significant reduction of cell viability and reduced mitochondrial transmembrane potential.
Deshmukh PS et al. · 2013
The study investigated possible DNA-damaging effects of low-level microwave radiation in the brains of male Fischer rats. Rats were exposed for 30 days at three frequencies: 900, 1800, and 2450 MHz, at specific absorption rates on the order of 10−4 W/kg. DNA damage in brain tissue was assessed using alkaline comet assay after the exposure period. The researchers concluded that low SAR microwave radiation at these frequencies may induce DNA strand breaks in brain tissue.
