Unknown authors · 1996
Rats were trained in ten daily sessions on a 12-arm radial maze to assess spatial memory. Exposure to a 60 Hz magnetic field at 0.75 mT for 45 minutes before each session significantly retarded learning.
Unknown authors · 1996
Researchers at the University of Washington exposed rats to 60 Hz magnetic fields (the same frequency as power lines) for 45 minutes before each training session in a maze test. The magnetic field exposure significantly impaired the rats' ability to learn spatial navigation tasks. When researchers gave the rats a drug that boosts brain chemicals called cholinergics, it reversed the learning problems caused by the magnetic field.
Unknown authors · 1996
Researchers exposed rats to 60 Hz magnetic fields (the same frequency as household electricity) for 45 minutes before training sessions in a spatial memory test. The magnetic field exposure significantly impaired the rats' ability to learn and navigate a maze. When researchers gave the rats a drug that boosts brain chemicals called cholinergic systems, it reversed the learning problems caused by the magnetic field.
Unknown authors · 1996
Researchers exposed human melanoma MeWo cells to a 50-Hz, 400-mT extremely low frequency magnetic field and measured mutations in the hypoxanthine-guanine phosphoribosyl transferase gene. The mutant frequency increased with longer exposure periods and higher induced current intensities. Mutations induced by X-rays were enhanced by the magnetic field exposure. No significant increase in mutant frequency occurred when DNA replication was inhibited during exposure.
Bawin SM, Satmary WM, Jones RA, Adey WR, Zimmerman G. · 1996
Researchers used rat hippocampal slices to study how extremely-low-frequency (ELF) magnetic fields affect rhythmic slow activity (RSA), a brain rhythm associated with memory functions. Slices were exposed for 10-minute epochs to 1 or 60 Hz magnetic fields at intensities of 5.6, 56, or 560 μT, or were sham exposed. 1 Hz fields at 56 and 560 μT, but not at 5.6 μT, triggered irreversible destabilization of RSA intervals, while 60 Hz fields showed similar but not statistically significant trends. The field effects on RSA did not occur when nitric oxide (NO) synthesis was pharmacologically inhibited.
Cleary, SF, Du, Z, Cao, G, Liu, LM, McCrady, C · 1996
Researchers investigated the effects of RF radiation exposure on interleukin 2 (IL-2) -dependent proliferation of cytolytic T lymphocytes (CTLL-2). At specific absorption rates greater than 25 W/kg, exposure produced a consistent, statistically significant reduction in CTLL-2 proliferation, especially at low IL-2 concentrations. At lower SARs, 2450 MHz exposure increased CTLL-2 proliferation immediately after exposure but reduced 24 h postexposure proliferation. The researchers found significant qualitative and quantitative differences between the effects of temperature elevation and RF radiation.
Wolke S, Neibig U, Elsner R, Gollnick F, Meyer R, · 1996
Unknown authors · 1996
Researchers transfected HeLa cells with plasmids containing c-fos upstream regulating regions up to -700 base pairs linked to a CAT reporter gene. The cells were exposed to a 60 Hz electromagnetic field at 60 mGrms. CAT expression increased above control levels after 5 minutes of exposure, peaked at 20 minutes, and returned to basal levels after 40 minutes. Deletion analysis identified a responsive region of 138 base pairs from -363 to -225 containing SRE/AP-1 sites.
Unknown authors · 1996
The study examined the effects of extremely low frequency magnetic fields on cell growth and c-myc mRNA expression in Chinese hamster ovary cells. The study tested a 5 mT, 60 Hz magnetic field. The exposure system consisted of a CO2 incubator with built-in Helmholtz coils (250 mm inner diameter, 160 mm distance, 128 turns), a slide regulator, and a thermocontroller. No significant difference was found in growth rate or c-myc expression between cells exposed to the magnetic field, sham-exposed cells, and cells in a conventional incubator.
Kittel A, Siklos L, Thuroczy G, Somosy Z · 1996
Unknown authors · 1996
The researchers investigated whether 60-Hz magnetic fields act at the cellular level to produce gene expression changes that can result in neoplastic transformation. Twenty-four hour exposure to 200 microT continuous magnetic fields produced negative results in Syrian hamster embryo cells and C3H/10T1/2 murine fibroblasts, with or without a chemical promoter. Extensive coded and uncoded analyses found no magnetic field effect on gene expression in human HL60 cells. No MF-induced changes in ornithine decarboxylase expression were observed in C3H/10T1/2 cells under the same exposure conditions.
Unknown authors · 1996
Exposure of breast cancer cells to 12 mG, 1 G, or 9 G 60-Hz electromagnetic fields failed to stimulate Cdk2 or cyclin B1-cdc2 activity or cyclin D1 synthesis. Simultaneous co-exposure to 60-Hz fields and chemical promoters did not enhance Cdk2 activation beyond the effect of the chemical promoter alone. Estrogen and xenoestrogens stimulated estrogen receptor binding to the estrogen receptor element, but EMF did not.
Unknown authors · 1996
Researchers tested whether 60 Hz electromagnetic fields (like those from power lines) could stimulate breast cancer cell growth or cause DNA damage in laboratory conditions. The study found that while estrogen and chemical estrogens promoted cancer cell division, electromagnetic field exposure at multiple intensities showed no effect on cell growth, gene activity, or DNA damage.
Detlavs I et al. · 1996
Researchers exposed Wistar rats with full-thickness dermal wounds to RF EMF for 30 min daily during the first 5 days using 53.53 GHz without modulation, 42.19 GHz without modulation, and 42.19 GHz with a 200-MHz frequency modulation band. RF EMF without frequency modulation decreased glycoprotein macromolecules and reduced inflammatory exudation and total collagen accumulation. RF EMF with frequency modulation significantly elevated hexoses and sialic acid concentrations, indicating intensified exudative phenomena, and increased collagenous protein accumulation. The authors concluded that non-thermal EMF affects reparative-proliferative processes in soft tissue wounds.
Unknown authors · 1996
Researchers treated cultured mammalian cells with three carcinogens that produce DNA single-strand breaks. The repair rate of these lesions was unchanged by 50 Hz electric, magnetic, or combined fields across a range of intensities.
Elekes E, Thuróczy G, Szabó LD. · 1996
Researchers tested the effect of continuous or amplitude-modulated 2.45 GHz microwave radiation on the immune response of mice. CW exposures induced elevations of the number of antibody-producing cells in the spleen of male Balb/c mice by 37%. AM microwave exposure induced elevation of the spleen index by 15% and antibody-producing cell number by 55% in the spleen of male mice. No changes were observed in female mice.
Elekes, E, Thuroczy, G, Szabo, LD · 1996
Researchers exposed male and female mice to microwave radiation at 2.45 GHz (similar to microwave ovens and WiFi) for 3 hours daily over 6 days to test effects on immune function. They found that both continuous and pulsed microwave exposure significantly increased antibody production in male mice (37-55% increases), but had no effect on female mice. This suggests that microwave radiation can stimulate immune system activity, with males appearing more sensitive than females.
Kubinyi G, Thuroczy G, Bakos J, Boloni E, Sinay H, Szabo LD, · 1996
Pregnant CFLP mice were exposed to 2.45 GHz microwave radiation for 100 minutes each day throughout gestation in an anechoic chamber. Brain aminoacyl-tRNA synthetase activity decreased significantly after continuous-wave exposure but not after amplitude-modulated exposure. Liver enzyme activity increased after both continuous-wave and amplitude-modulated exposure, while body and organ weights were unaffected.
Urech, M, Eicher, B, Siegenthaler, J · 1996
Researchers conducted field experiments lasting one to three years alongside laboratory tests, exposing Parmelia tiliacea and Hypogymnia physodes to 2.45 GHz microwaves at 0.2, 5, and 50 mW/cm² with a control. Both species showed a substantially reduced growth rate at the highest exposure of 50 mW/cm². Temperature measurements revealed that microwaves at 2.45 GHz and 50 mW/cm² substantially increased lichen surface temperature and accelerated drying. When the same species were exposed near a 9.5 MHz short-wave broadcast transmitter at up to 235 V/m, no visible effects were detected.
Unknown authors · 1996
Researchers investigated interactions of weak ELFMFs with primitive haemopoietic cells in vitro using the multipotential progenitor cell line FDCP-mix(A4). They determined proliferative activity and clonogenic potential under optimal and sub-optimal growth conditions with exposures to ambient laboratory ELFMFs or three other ELFMF regimes including nulled fields, Ca2+-ion cyclotron resonance at 50 Hz, and vertical 50 Hz fields of 6 muT(RMS). Using exposures of 1, 4, 7 and 21 days, they found no significant alteration of growth rate, cell-cycle state or clonogenic efficiency.
Unknown authors · 1996
Researchers exposed blood stem cells to extremely low frequency magnetic fields similar to those from power lines for up to 21 days. The fields had no effect on cell growth, division, or ability to form colonies. This laboratory study found no evidence that power line EMF directly promotes blood cancer development in these primitive blood cells.
Singh B, Bate LA · 1996
Researchers exposed pigs to infrared, low-power (6.1 mW cm-2), and high-power (11.4 mW cm-2) 915 MHz microwave radiation for 24 hours, with controls under natural light. Lung tissues were examined ultrastructurally and for acid phosphatase activity, and rough endoplasmic reticulum fraction was measured. Low-power microwave exposure increased the rough endoplasmic reticulum to cytoplasm ratio, prominent Golgi profiles, and secretory vesicles in pulmonary intravascular macrophages compared to other groups. High-power microwave exposure caused extracellular acid phosphatase precipitates and disrupted collagen matrix in the pulmonary interstitium. Intracellular globules appeared in infrared and low-power microwave groups but not in controls or high-power group.
Pashovskina MS, Akoev IG · 1996
A solution of rat muscle actomyosin was exposed to 2375 MHz pulse-modulated microwave radiation with rectangular pulse modulation ranging from 50 to 300 pulses per second. The study found that actomyosin ATPase activity was dependent on both modulation frequency and microwave intensity. Specific modulation frequencies were found to change the ATPase activity of actomyosin.
Bortkiewicz A, Gadzicka E, Zmyslony M, · 1996
The study compared 71 workers at medium-frequency broadcast stations with 22 non-exposed radio link station workers. Age and work tenure distributions did not differ significantly between groups. Heart rate variability was measured from 512 normal heart evolutions at rest using a Medea-HV system. Time-domain and frequency-domain HRV parameters showed no statistically significant differences between exposed and non-exposed groups. In the exposed group, maximum EM field intensity correlated negatively with high-frequency power spectrum.
Sparks PB, Mond HG, Joyner KH, Wood MP · 1996
Researchers exposed 16 implanted Telectronics META pulse generators to simulated GSM transmissions and a 2-W digital mobile telephone. The devices were programmed to maximum sensitivity and tested in unipolar and bipolar settings and rate adaptive and nonrate adaptive modes. At maximum sensitivity, 11 of 16 devices showed no EMI, while brief ventricular triggering, pauses, and one episode of pacemaker-mediated tachycardia occurred in the remaining devices. When programmed to routine sensitivities, only one device displayed rare single beat ventricular triggering, and no changes in minute ventilation rate adaptive pacing were observed.