Unknown authors · 2004
They measured cytoplasmic free calcium using the ratiometric fura-2 technique and mitochondrial membrane potential using the JC-1 probe. Exposure caused a highly significant increase in DNA strand breaks measured by the comet assay. No changes were observed in JC-1 fluorescence or cytoplasmic free calcium compared to sham-exposed cells.
Vorobyov V, Pesic V, Janac B, Prolic Z. · 2004
Researchers exposed rats repeatedly for three days to low-intensity 915 MHz microwaves modulated at 4 Hz and recorded EEG from the somatosensory cortex. Microwave exposure alone significantly enhanced fast EEG rhythms (18-30 Hz), an effect not seen in sham-exposed or radiation-naïve rats. In microwave-exposed rats, scopolamine did not cause the EEG slowing observed in non-exposed rats, and its effect resembled that of physostigmine in naive animals.
Busljeta I, Trosic I, Milkovic-Kraus S. · 2004
The study observed erythropoietic changes in rats subchronically exposed to radiofrequency microwave radiation at a nonthermal level. Adult male Wistar rats were exposed to 2.45 GHz continuous RF/MW fields for 2 hours daily, 7 days a week, at 5 – 10 mW/cm2. In exposed animals, erythrocyte count, haemoglobin and haematocrit were increased in peripheral blood on irradiation days 8 and 15. Anuclear cells and erythropoietic precursor cells were significantly decreased in the bone marrow on day 15, while micronucleated cells' frequency was increased.
Trosic I, Busljeta I, Modlic B. · 2004
Researchers used an erythrocyte micronucleus assay to investigate genetic damage in bone marrow red cells of rats exposed to radiofrequency/microwave radiation. Wistar rats were exposed to a 2.45 GHz continuous field for 2 hours daily, 7 days a week, at a power density of 5–10 mW/cm2, with a whole body average specific absorption rate calculated to be 1.25 ± 0.36 W/kg. Significant differences in polychromatic erythrocytes were found on experimental days 8 and 15, and the frequency of micronucleated polychromatic erythrocytes was also significantly increased on day 15. Comparisons after 2, 8, and 30 irradiation treatments did not reveal statistically significant differences between sham-exposed and treated subgroups.
Sarimov R et al. · 2004
Researchers exposed lymphocytes from seven healthy persons to GSM microwaves at different frequencies in the range of 895-915 MHz and measured changes in chromatin conformation. A 30-min exposure at 900 and 905 MHz produced statistically significant condensation of chromatin in lymphocytes from 1 of 3 tested donors. Stronger effects were found following 1-h exposure, with chromatin condensation in 3 donors and decondensation in 1 donor. The observed chromatin changes were similar to effects of heat shock within the temperature window of 40/spl deg/C-44/spl deg/C.
Koyama S et al. · 2004
Researchers tested whether an extremely low-frequency magnetic field (ELFMF) could affect mutations caused by hydrogen peroxide in pTN89 plasmids. Mutations were detected in the supF gene carried by the plasmids in Escherichia coli. The mutation frequency was 2.28×10−4 for hydrogen peroxide treatment alone, and 5.81×10−4 when the ELFMF was applied at the same time. No mutations were observed with ELFMF exposure alone, and the researchers indicated that the ELFMF may potentiate hydrogen peroxide-induced mutation.
Lee BC et al. · 2004
Researchers investigated whether a 60 Hz magnetic field could affect the antioxidant defense system in mouse brain by measuring chemiluminescence and superoxide dismutase (SOD) activity in brain homogenates. A chemiluminescence measure linked to oxidative stress was significantly increased in mice exposed to 60 Hz, 12 G for 3 hours compared to the sham-exposed control group. Brain SOD activity was also significantly increased in the exposed group. The researchers suggest these results indicate the field could deteriorate the antioxidant defensive system through reactive oxygen species other than superoxide radicals.
Rollwitz J, Lupke M, Simkó M · 2004
Researchers studied the effects of 50 Hz magnetic fields on free radical production in mouse bone marrow-derived macrophages and monocytes. Exposure to the magnetic fields at a flux density of 1 mT produced a significant increase in free radical production in both cell types. The radicals produced were mainly superoxide anion radicals, found in macrophages at 33% and in their precursor cells at 24%.
Anderson LE et al. · 2004
Researchers set out to determine whether long-term exposure to a 1.6 GHz radiofrequency field would affect cancer incidence in Fischer 344 rats. Offspring were exposed to near-field radiofrequency signals at brain SAR levels of 1.6 W/kg or 0.16 W/kg for 2 hours per day, 5 days per week, from about 36 days old until they were 2 years old. No statistically significant differences were found among treatment groups for neoplastic lesions or other clinical signs.
Cassel JC, Cosquer B, Galani R, Kuster N. · 2004
Rats were tested in a 12-arm radial-maze after daily exposure to 2.45 GHz microwaves for 45 minutes at a SAR of 0.6 W/kg. The performance of exposed rats was comparable to that found in sham-exposed or in naive rats.
Sarimov et al. · 2004
Researchers exposed human white blood cells (lymphocytes) to cell phone radiation at levels similar to what phones emit during calls. They found that 30-60 minutes of exposure caused changes in the DNA packaging inside cells that were similar to heat stress damage. These cellular changes occurred in most test subjects and suggest that phone radiation may trigger stress responses in our immune cells even at low power levels.
Sarimov et al. · 2004
Researchers exposed human immune cells to cell phone radiation at typical frequencies for 30 minutes to 1 hour. The extremely weak signals - 200 times below safety limits - triggered DNA packaging changes resembling heat stress in most subjects, suggesting cellular effects from everyday phone use.
Ilhan A et al. · 2004
Researchers investigated the effect of Ginkgo biloba on mobile phone-induced oxidative damage in rat brain tissue. Mobile phone exposure increased malondialdehyde, nitric oxide, xanthine oxidase, and adenosine deaminase while decreasing superoxide dismutase and glutathione peroxidase activities in brain tissue. Ginkgo biloba treatment prevented these oxidative alterations and the mobile phone-induced cellular injury in brain tissue.
Demsia G, Vlastos D, Matthopoulos DP. · 2004
The study aimed to investigate whether 910-MHz electromagnetic fields could be a noxious agent capable of inducing genotoxicity, and rats were exposed daily for 2 h over 30 consecutive days. Researchers examined bone marrow smears from EMF-exposed and sham-exposed animals and observed an almost threefold increase in micronuclei in polychromatic erythrocytes. An induction of micronuclei was also observed in polymorphonuclear cells.
Lagroye I et al. · 2004
Researchers exposed C3H 10T½ cells to 2450 MHz CW microwaves at 1.9 W/kg for 2 hours, then irradiated them with 4 Gy gamma rays, and used the single-cell gel electrophoresis assay to detect DNA damage. Ionizing radiation induced significant DNA damage, but microwave exposure alone caused no detectable DNA damage. Microwaves did not impede DNA migration induced by gamma rays, and no evidence of DNA–protein crosslinks or changes in DNA-associated protein was found after microwave exposure.
Ono T et al. · 2004
A possible mutagenic effect of 2.45 GHz radiofrequency exposure was examined using lacZ-transgenic MutaTM mice. Offspring were exposed in utero for 16 hours a day from embryonic day 0 to 15 and examined at 10 weeks of age. Mutation frequencies at the lacZ gene in spleen, liver, brain, and testis were similar to those in non-exposed mice. Sequencing of mutant DNAs showed no appreciable difference in mutation quality between exposed and non-exposed samples.
Unknown authors · 2004
Researchers exposed cerebral cortical neurons from postnatal rats to low-intensity 900MHz continuous microwave electromagnetic fields at 9mW/cm2 with a SAR of 3.22mW/g for 2 hours per day over 4 or 5 consecutive days and also for 12 hours at a time. They measured cytochrome oxidase activity as a marker of energy metabolism. Compared to sham-exposed neurons, cytochrome oxidase activity decreased significantly in neurons exposed for 2 hours per day and in those exposed for 12 hours (P < 0.001).
Unknown authors · 2004
Chinese researchers exposed developing rat brain neurons to 900 MHz microwave radiation (the frequency used by many cell phones) and found significant decreases in cellular energy production. The study showed that even low-intensity exposure damaged the neurons' ability to generate energy, with effects occurring after just 2 hours of daily exposure.
Unknown authors · 2004
Chinese researchers exposed developing rat brain neurons to 900 MHz microwave radiation (the same frequency used by many cell phones) and found significant decreases in cytochrome oxidase activity, a key enzyme for cellular energy production. The effects occurred at power levels similar to those from mobile devices and persisted even with brief daily exposures over several days.
Unknown authors · 2004
Unknown authors · 2003
Researchers exposed cultured human diploid fibroblasts to intermittent ELF electromagnetic fields. DNA damage was measured using alkaline and neutral comet assays. Exposure at 50 Hz sinusoidal, 1-24 h, 20-1,000 mu T, with a 5 min on/10 min off pattern induced dose-dependent and time-dependent DNA single-strand and double-strand breaks. Effects were observed at magnetic flux densities as low as 35 mu T, well below ICNIRP guidelines. The induced comet tail factors returned to normal within 9 h after exposure ended.
Unknown authors · 2003
Austrian researchers exposed human skin cells to 50 Hz electromagnetic fields (the same frequency as power lines) and found it caused DNA breaks in both single and double strands. The damage occurred at magnetic field strengths as low as 35 microTesla, well below international safety guidelines, and got worse with higher exposures and longer duration.
Unknown authors · 2003
Austrian researchers exposed human skin cells to 50 Hz electromagnetic fields (the same frequency as power lines) and found DNA damage occurred in a dose-dependent manner. The damage included both single and double-strand DNA breaks, starting at magnetic field levels as low as 35 microTesla - well below current safety guidelines. This provides laboratory evidence supporting epidemiological studies linking power line EMF exposure to increased cancer risk.
Unknown authors · 2003
This study examined cultured fibroblasts from six donors of varying ages exposed to 50 Hz ELF-EMF at 1 mT for 1-24 hours. Basal DNA strand break levels correlated with donor age, with maximum response observed at 15-19 hours of exposure, and this response was more pronounced in cells from older donors.
Haarala C et al. · 2003
Sixty-four subjects (32 men, 32 women) in two independent laboratories performed nine cognitive tasks twice, once with the EMF on and once with it off. Reaction times and accuracy were recorded, and the order of exposure and tasks was counterbalanced across subjects and gender. There were no statistically significant differences in performance between genders or laboratories. The previous results were not replicated, and the researchers concluded that EMF had no effect on reaction times or accuracy.