Franzellitti S et al. · 2010
Researchers exposed human trophoblast HTR-8/SVneo cells to 1.8 GHz continuous wave and GSM signals for 4, 16, or 24 hours. The GSM-Talk signal used an intermittent pattern of 5 minutes on and 10 minutes off. The alkaline comet assay measured primary DNA damage and strand breaks from incomplete repair. Amplitude-modulated GSM-217 Hz and GSM-Talk signals significantly increased comet parameters after 16 and 24 hours, while continuous wave did not. DNA damage recovered within 2 hours after exposure ended, returning to sham-exposed levels.
Patruno A et al. · 2010
The study analyzed the effect of ELF-EMF on the human keratinocyte cell line HaCaT to assess mechanisms and support therapeutic use in wound healing. Exposure to ELF-EMF increased iNOS and eNOS expression levels, NOS activities, and NO production. Higher AP-1 expression and cell proliferation rate were also associated with ELF-EMF exposure. In contrast, ELF-EMF decreased COX-2 expression, PGE2 production, catalase activity, and O2− production.
Vecchio F et al. · 2010
Researchers recorded eyes-closed resting EEG data from 16 healthy elderly subjects and 5 young subjects during GSM-on and GSM-off conditions lasting 45 minutes each. Spectral coherence measured inter-hemispheric synchronization across delta, theta, and alpha frequency bands. Elderly subjects showed a statistically significant increment of inter-hemispheric coherence of frontal and temporal alpha rhythms during the GSM condition compared to young subjects. The results suggest GSM-EMFs affect inter-hemispheric synchronization of alpha EEG rhythms as a function of physiological aging.
Panda NK, Jain R, Bakshi J, Munjal S. · 2010
Participants underwent a battery of audiologic investigations including pure-tone audiometry, tympanometry, distortion product otoacoustic emissions, auditory brain responses, and middle latency responses. There was no significant difference between users and controls for any of the audiologic parameters. However, trends for audiologic abnormalities were seen within users, with high-frequency loss and absent distortion product otoacoustic emissions observed with longer duration of use, excessive use, and age over 30 years. The researchers concluded that long-term and intensive mobile phone use may cause inner ear damage, but a larger sample size would be needed for definitive conclusions.
Hardell L, Söderqvist F, Carlberg M, Zetterberg H, Mild KH · 2010
Researchers studied the concentration of β-trace protein in blood in relation to emissions from wireless phones in 62 persons aged 18-30 years. The concentration of β-trace protein decreased with increasing number of years of use of a wireless phone, yielding a negative β coefficient of -0.32 with a 95% confidence interval of -0.60 to -0.04. Cumulative use in hours also gave a negative β coefficient, although not statistically significant. Forty of the 62 persons participated in an experimental study with 30 min exposure to an 890-MHz GSM signal, and no statistically significant change of β-trace protein was found. In a similar study of the remaining 22 participants with no exposure, β-trace protein increased significantly over time, probably due to a relaxed situation.
Vorobyov V, Janać B, Pesić V, Prolić Z. · 2010
Researchers studied EEG in 10 freely moving rats with electrodes implanted in the cortex and dorsomedial hypothalamus over five consecutive days. Rats were exposed to ELF-MW (915 MHz, 20-ms pulse duration, approximately 0.3 mW/cm(2), 4 Hz) intermittently (1-min 'On', 1-min 'Off') for 10 min several times per day. Baseline EEG showed dominant activity in the 3.2-6.0 Hz and 17.8-30.5 Hz ranges in the cortex, and in the 6.0-17.8 Hz range in the hypothalamus. ELF-MW exposure increased beta(2) activity in the hypothalamus more than in the cortex, significantly reducing the initial cortical-hypothalamic EEG imbalance. A cumulative effect was observed across repeated exposures.
Panagopoulos DJ, Chavdoula ED, Margaritis LH · 2010
Researchers exposed adult Drosophila melanogaster to GSM 900/1800 mobile phone antenna radiation at distances from 0 to 100 cm and measured reproductive capacity and cell death in the gonads using the TUNEL assay. The radiation decreased reproductive capacity by inducing cell death at all distances tested. The effect was strongest at 20-30 cm (radiation intensity around 10 microW/cm(2)), diminished considerably beyond 40-50 cm, and was not evident beyond 1 m or below 1 microW/cm(2).
Kim BC, Park SO. · 2010
Researchers measured human exposure to CDMA800 and CDMA1800 signals at 50 locations across Korea where the general public had expressed concern. Measurement positions were within approximately 32 to 422 m of the nearest base station at each site. The measured exposure levels were very low compared with the international standard and the Korean human protection notice, with the highest field level at 1.5 V/m.
Kawai H, Nagaoka T, Watanabe S, Saito K, Takahashi M, Ito K. · 2010
Researchers used computational models of pregnant women at 4 and 8 weeks of pregnancy to calculate the specific absorption rate (SAR) in embryos exposed to electromagnetic plane waves from 10 MHz to 1.5 GHz. The models had a 2 mm spatial resolution and represented a woman similar in height and weight to an average-sized Japanese adult female, with either a cubic 4-week or spheroidal 8-week embryo. The maximum average SAR in the embryos was lower than 0.08 W kg(-1) when the incident power density was at the ICNIRP reference level for the general public.
Joseph W, Vermeeren G, Verloock L, Martens L. · 2010
Researchers converted personal electromagnetic field measurements into whole-body specific absorption rates (SAR) for members of the general public. Among the body types compared, the highest SAR values at 950 MHz were found for the 1-year-old child, followed by the 5-year-old child, 10-year-old child, average woman, and average man. All calculated values remained below the basic restriction of 0.08 W/kg for the general public.
Hirata A et al. · 2010
Researchers exposed rabbits to 2.45-GHz microwaves across a range of power densities to study the threshold for behavioral signs of thermal stress. The study found that a core temperature elevation of 1 degrees C was an estimate of the threshold for inducing behavioral signs of microwave-induced thermal stress in rabbits. The whole-body average SAR needed to produce behavioral signs was estimated at approximately 1.3 W/kg.
Gerner C et al. · 2010
Researchers used proteome analysis to study whether low-intensity radio-frequency electromagnetic field exposure could affect protein synthesis in cultured human cells. Four different cell types were exposed to 2 W/kg specific absorption rate, and autoradiography of 2D gel spots was used to measure changes in synthesis of individual proteins. An 8-hour exposure caused a significant increase in protein synthesis in Jurkat T-cells and human fibroblasts, and to a lesser extent in activated primary human mononuclear cells, while short-term exposure did not significantly alter the proteome. Quiescent, metabolically inactive mononuclear cells did not detectably respond to the exposure.
Frei P et al. · 2010
Researchers collected personal exposimeter measurements during one week and diary data from 166 study participants to evaluate different methods of assessing radio frequency electromagnetic field exposure. The mean personal exposure was 0.13 mW/m(2) when measurements during personal phone calls were excluded and 0.15 mW/m(2) when such measurements were included. Self-estimated exposure and geo-coded distance turned out to be poor surrogates for personal exposure.
Findlay RP, Dimbylow PJ. · 2010
Researchers calculated specific energy absorption rate (SAR) values in a 10-year-old sitting voxel model from exposure to electromagnetic fields at 2.4 and 5 GHz, frequencies commonly used by Wi-Fi devices. Both plane-wave exposure and irradiation from antennas in the near field were investigated for a variety of exposure conditions. In all situations studied, the calculated SAR values were considerably below basic restrictions.
Augner C et al. · 2010
Researchers tested whether RF-EMF emitted by mobile phone base stations may affect salivary alpha-amylase, immunoglobulin A, and cortisol levels. Fifty-seven participants were randomly allocated to one of three experimental scenarios, and each participant went through five 50-minute exposure sessions. When exposure shifted from low to high in scenario 3, an increase of cortisol was detected, and IgA concentration was not significantly related to the exposure. The researchers suggested that RF-EMF in considerably lower field densities than ICNIRP guidelines may influence certain psychobiological stress markers.
Zhijian C et al. · 2010
Researchers used a comet assay to study whether 1.8-GHz radiofrequency radiation (RFR, SAR of 2 W/kg) could influence DNA repair in human B-cell lymphoblastoid cells exposed to doxorubicin (DOX). RFR alone did not directly induce DNA damage in the cells. A specific combined-exposure pattern could obviously influence DNA repair at 6 h and 12 h after exposure to DOX for four DOX doses.
Volkow ND et al. · 2010
Researchers measured brain glucose metabolism in fifteen healthy subjects inside a 4 T magnet, comparing conditions with and without echo-planar imaging (EPI) gradient pulses. Relative metabolism was lower in areas at the poles of the brain when the EPI pulses were on compared to when they were off. The size of the metabolic decreases was significantly correlated with the estimated strength of the electric field (r = 0.68, p < 0.0001), such that a stronger field was associated with larger decreases. No differences were detected between the ON and OFF conditions for mood ratings or for absolute whole-brain metabolism.
Ozgur E, Güler G, Seyhan N. · 2010
Researchers exposed guinea pigs to an 1800-MHz GSM-modulated signal at a whole body SAR of 0.38 W/kg for 10 or 20 minutes per day for seven days to study oxidative damage in the liver. After exposure, significant increases in malondialdehyde and total nitric oxide levels were observed, along with decreases in several antioxidant enzyme activities in the liver. The extent of oxidative damage was found to be proportional to the duration of exposure, and both NAC and EGCG were found to protect liver tissue against this damage.
Potenza L et al. · 2010
Researchers exposed human umbilical vein endothelial cells (HUVECs) to a 300 mT static magnetic field for 4, 24, 48, and 72 hours. After 4 hours of exposure, the static field induced damage at the nuclear and mitochondrial levels, reduced mitochondrial content, and increased reactive oxygen species. Twenty-four hours of exposure increased mitochondrial DNA content as well as expression of a gene related to mitochondrial biogenesis. No significant differences between exposed and sham cultures were found after 48 and 72 hours, and cell viability did not show a statistically significant change overall.
Martínez-Sámano J et al. · 2010
Researchers exposed adult male Wistar rats to extremely low frequency electromagnetic fields (60 Hz, 2.4 mT) for two hours to evaluate early effects on antioxidant systems in plasma, liver, kidney, and heart. SOD activity was lower in the plasma of restrained and EMF-exposed animals compared to unrestrained rats. There were no significant differences in CAT activity and TBARS levels among the experimental groups versus the control group. The researchers noted that oxidative stress induced by movement restraint was stronger than that produced by EMF.
Mannerling AC, Simkó M, Mild KH, Mattsson MO · 2010
Researchers exposed human K562 leukemia cells to a 50-Hz sine-wave magnetic field for 1 hour at flux densities ranging from 0.025 to 0.10 mT, measuring proliferation, cell cycle distribution, superoxide radical anion levels, and HSP70 protein levels. Both vertical and horizontal exposures caused significantly and transiently increased HSP70 levels of more than twofold at several flux densities, compared to sham controls and also compared to a heat-treatment positive control. Superoxide radical anion levels increased by 30–40%, and adding free radical scavengers melatonin or 1,10-phenantroline inhibited the magnetic-field-induced increase in HSP70. Cell cycle kinetics and proliferation were not affected by exposure at 0.10 mT for 1 hour.
Lee HM et al. · 2010
Researchers cultured human intervertebral disc (IVD) cells in alginate beads and exposed them to an electromagnetic field at 1.8 millitesla magnetic flux density and 60 Hz. Cellular proliferation was observed in the EMF group (p < 0.05), and no cytotoxicity was found. There was no significant change in the expression of aggrecan, type I, and type II collagen mRNA in the EMF group compared to the control group. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2.
Garip AI, Akan Z. · 2010
Researchers investigated the effect of ELF-EMF on apoptosis in K562 human leukemia cells, with and without oxidative stress, and examined links to heat-shock protein 70 levels. ELF-EMF at 1 mT and 50 Hz was applied for 3 hours, with one group of cells treated with H2O2 to induce oxidative stress and another left untreated. In cells not exposed to oxidative stress, ELF-EMF caused a decrease in the number of apoptotic cells and a slight increase in viability. In cells treated with H2O2, ELF-EMF increased the number of apoptotic cells, and hsp70 and reactive oxygen species levels were also increased.
Akan Z, Aksu B, Tulunay A, Bilsel S, Inhan-Garip A · 2010
Researchers applied 50 Hz, 1 mT electromagnetic fields for 4 to 6 hours to human monocytic leukemia cells (THP-1) that had been stimulated with Staphylococcus aureus or interferon gamma/lipopolysaccharide. Field application increased nitric oxide levels, with the increase appearing earlier and being more prominent in cells stimulated with S. aureus. ELF-EMF alone caused increased hsp70 levels in a time-dependent manner, and the growth curve of exposed bacteria was lower than the control. The researchers suggested that ELF-EMF could be exploited for beneficial uses.
Szemerszky R, Zelena D, Barna I, Bárdos G. · 2010
Researchers exposed adult male Sprague-Dawley rats to 50 Hz electromagnetic fields at 0.5 mT, either for 5 days at 8 hours daily or for 4 to 6 weeks at 24 hours daily. Neither exposure duration produced the body changes associated with chronic stress, such as body weight reduction, thymus involution, or adrenal gland hypertrophy. After long-term exposure, rats showed elevated proopiomelanocortin and depressive-like behavior, and blood glucose was also higher. The researchers noted that the exposure intensity used was much higher than typical human exposure.
