de Souza FT et al. · 2014
Researchers investigated whether cell phone use alters the expression of gene products related to cellular stress in parotid glands. They used saliva from 62 individuals, comparing the gland exposed to cell phone radiation (ipsilateral) to the unexposed contralateral gland. No differences were found in salivary flow, total protein concentration, p53, p21, ROS, GSH, heat shock proteins 27 and 70, or IgA, even when grouping by years of use or monthly call minutes.
de Groot MW, Kock MD, Westerink RH. · 2014
Researchers investigated acute (30 min) and sub-chronic (48 h) exposure to 50 Hz ELF-EMF on naïve and chemically stressed PC12 cells. Acute exposure up to 1000 μT did not affect basal or depolarization-evoked calcium levels in naïve cells. Sub-chronic exposure up to 1000 μT had no consistent effects on calcium homeostasis, ROS production, or membrane integrity in naïve cells. In chemically stressed cells, both acute and sub-chronic exposure also failed to exert consistent effects on calcium homeostasis, ROS production, and membrane integrity. The authors concluded that exposure up to 1000 μT does not induce neurotoxic effects in vitro in either cell type.
Vereshchako GG, Chueshova NV, Gorokh GA, Naumov AD. · 2014
Researchers studied the effects of prolonged 897 MHz cell phone radiation exposure on male rats from irradiated parents during embryogenesis and postnatal development. Irradiation decreased the number of births and shifted the sex ratio toward more males. Radiation accelerated sexual development at two months, caused disproportions in spermatogenic cell stages, and increased mature sperm count while reducing their viability.
Tök L, Nazıroğlu M, Doğan S, Kahya MC, Tök O. · 2014
Thirty-two rats were randomly assigned to four groups, with one group exposed to Wi-Fi at 2.45 GHz for 60 minutes daily over 30 days and another group receiving melatonin during the same exposure. Lipid peroxidation was slightly higher in the Wi-Fi group than in controls, and glutathione peroxidase activity was significantly lower in the Wi-Fi group. Melatonin supplementation significantly decreased lipid peroxidation and increased both glutathione peroxidase activity and reduced glutathione levels compared with the Wi-Fi group.
Marzook EA, Abd El Moneim AE, Elhadary AA. · 2014
Triglycerides and testosterone increased significantly in all exposed groups, with a dose-dependent rise in the sesame oil groups. Superoxide dismutase and catalase activities decreased in exposed rats but increased with higher sesame oil doses. Total cholesterol decreased only in rats receiving 3 ml of sesame oil, and glutathione reductase rose significantly in that group. High-density and low-density lipoprotein cholesterol changed in opposite directions between the exposed and sesame oil groups.
Luo YP, Ma HR, Chen JW, Li JJ, Li CX. · 2014
Researchers randomly assigned 40 male SD rats to a normal control group, a model group, a Shuifei Jibin Capsule group, and an American Ginseng Capsule group, with 10 rats each. Rats in the model, SJC, and AGC groups were exposed to imitated 900 MHz cellular phone radiation for 4 h in 12 consecutive days, while the normal control group was not irradiated. Compared to the normal control, the model group showed hepatocyte nuclear atrophy, increased liver MDA and Nrf2 protein, and decreased SOD and GSH. In the SJC group, liver MDA and Nrf2 decreased and SOD, GSH, and GSH-PX increased; in the AGC group, MDA and Nrf2 decreased and SOD and GSH increased.
Burlaka A et al. · 2014
Yi G, Wang J, Wei X, Deng B, Tsang KM, Chan WL, Han C. · 2014
The study used a conductance-based neuron model with ELF sinusoidal magnetic field as an additive voltage input. Neurons with distinct spiking patterns showed different response properties to magnetic field exposure. For tonic spiking neurons, perturbations were maximized at harmonics of intrinsic spiking frequency, while for burst spiking neurons they were maximized at harmonics of bursting frequency. Perturbations increased with magnetic field intensity, and bursting dynamics were less sensitive than tonic spiking to ELF magnetic field exposure. ELF magnetic field exposure was more likely to perturb spike times than spiking frequency.
Ozgur E, Guler G, Kismali G, Seyhan N · 2014
Researchers exposed Hep G2 hepatocarcinoma cells to intermittent 900- and 1800-MHz radiofrequency radiation at a specific absorption rate of 2 W/kg for 1, 2, 3, or 4 hours. Cell viability changed according to the frequency and duration of the exposure. Four-hour exposure produced more pronounced effects than shorter durations. 1800-MHz radiation had a larger impact on cell viability and injury than 900-MHz radiation. Morphological analysis showed irregular nuclei and DNA damage after 4 hours of 1800-MHz exposure.
Margaritis LH et al. · 2014
Researchers exposed newly emerged flies daily for 3-7 days to various EMF sources including mobile phone, DECT, Wi-Fi, Bluetooth, and others. All EMF sources produced statistically significant effects on fecundity and apoptotic cell death, even at very low intensity levels such as 0.3 V/m from Bluetooth. The study found no linear cumulative effect when increasing exposure duration or combining sources, and discussed the role of average versus peak electric field values.
Kahya MC, Nazıroğlu M, Ciğ B. · 2014
Researchers studied the effects of 900 MHz radiation on oxidative stress, apoptosis, and mitochondrial depolarization in a breast cancer cell line. Cells were exposed to 900 MHz radiation for 1 hour at a SAR value of 0.36 ± 0.02 W/kg, and some groups were also incubated with sodium selenite before exposure.
Canseven AG, Esmekaya MA, Kayhan H, Tuysuz MZ, Seyhan N. · 2014
Raji cells were exposed to microwave radiation at a specific absorption rate of 0.350 W/kg for 24 hours. Microwave exposure alone significantly increased apoptotic activity and significantly decreased cell viability, and combined exposure with Gemcitabine increased apoptotic cells further than microwave radiation alone.
Alon L, Cho GY, Yang X, Sodickson DK, Deniz CM. · 2014
Researchers investigated MR temperature mapping and 10-g average specific absorption rate (SAR) computation for safety evaluation of RF-emitting devices. They tested an MRI-compatible dipole antenna and a non-MRI-compatible mobile phone, measuring temperature changes in phantoms and comparing results with physical probe measurements and electromagnetic field simulations. The researchers concluded that MR temperature mapping alongside physical property measurements can assess RF/microwave safety with high resolution and fidelity.
Ying Li and Paul Heroux · 2014
Researchers exposed five cancer cell lines to extra-low-frequency magnetic fields in the range of 0.025 to 5 µT and examined them for karyotype changes after 6 days. All cancer cell lines lost chromosomes from magnetic field exposure, with a mostly flat dose-response. The researchers suggest these karyotype contractions are caused by magnetic field interference with mitochondria's adenosine triphosphate synthase, compensated by the action of adenosine monophosphate-activated protein kinase. Constant magnetic field exposures for three weeks allowed a rising return to baseline, unperturbed karyotypes, but small increases or decreases in field strength were again capable of inducing karyotype contractions.
Pelletier SJ et al. · 2014
Researchers tested various intensities of direct current electric fields (0, 25, 50, and 100V/m) on neurons, microglia, and astrocytes in a controlled laboratory setting. Neurons developed an elongated cell body shape with neurite outgrowth associated with a significant increase in growth associated protein-43. BV2 microglial cells showed distinct morphological changes with an increase in cyclooxygenase-2 expression, and these changes depended on whether the cells had already been activated with lipopolysaccharide. Astrocytes displayed elongated cell bodies with cellular filopodia that were oriented perpendicularly to the direct current electric field.
Sefidbakht Y et al. · 2014
Researchers exposed HEK293T cells carrying a firefly luciferase gene to 940 MHz RF-EMF at a mean specific absorption rate of approximately 0.09 W kg-1. The effects of exposure durations of 15, 30, 45, 60 and 90 minutes on luciferase activity and oxidative response elements were investigated. Luciferase activity was reduced after 30 and 45 minutes of continuous exposure, while after 60 minutes the exposed cells showed higher luciferase activity compared with non-exposed controls. ROS generation was highest in the 30-minute exposed cells, followed by a sharp rise in catalase and superoxide dismutase activity and elevation of glutathione during the 45-minute exposure.
Manta AK, Stravopodis DJ, Papassideri IS, Margaritis LH. · 2014
Researchers measured reactive oxygen species (ROS) levels in the bodies and ovaries of 4-day-old Drosophila melanogaster flies after exposing them to DECT base radiation at 1.88–1.90 GHz. Male and female bodies showed a twofold increase in ROS levels after 6 hours of exposure, with ROS levels returning to normal 24 hours after that exposure ended. Ovaries responded more quickly, reaching a 2.5-fold ROS increase after 1 hour of exposure, with levels returning to normal 4 hours after that exposure ended.
Abu Khadra KM, Khalil AM, Abu Samak M, Aljaberi A. · 2014
Researchers evaluated biochemical parameters in the saliva of 12 young males before and after using a mobile phone. The phone emitted radio frequency signals of 1800 MHz with a 1.09 w/kg specific absorption rate, and participants used it for 15 and 30 minutes. SOD levels followed a quadratic trend, rising after 15 minutes of talking but then dropping as talking time increased. The researchers suggested that exposure to electromagnetic radiation may exert an oxidative stress on human cells, as evidenced by the increase in the concentration of the superoxide radical anion released in the saliva of cell phone users.
Pandir D, Sahingoz R · 2014
Researchers exposed Mediterranean flour moth (Ephestia kuehniella) larvae to a 1.4-Tesla magnetic field at 50 Hz for time periods ranging from 3 to 72 hours. DNA damage, measured by the comet assay, was significantly larger than in the control group at increasing exposure times. Antioxidant enzyme activities (SOD, CAT, GPx, and GST) decreased and the lipid peroxidation marker MDA increased in exposed larvae compared to controls.
Chen C et al. · 2014
Researchers exposed embryonic neural stem cells (eNSCs) to 1800 MHz radiofrequency electromagnetic fields at SAR values of 1, 2, and 4 W/kg for 1, 2, and 3 days. RF-EMF exposure did not influence eNSC apoptosis, proliferation, cell cycle, or the ratio of differentiated neurons and astrocytes. However, neurite outgrowth of eNSC-derived neurons was inhibited after 4 W/kg RF-EMF exposure for 3 days. The mRNA and protein expression of proneural genes Ngn1 and NeuroD were decreased after RF-EMF exposure, while expression of their inhibitor Hes1 was upregulated.
Unknown authors · 2013
The study investigated HSP70 responses in transfected rat primary fibroblast cells exposed to 10 Hz electric fields at intensities of 20–500 V/m. Low frequency electric fields induced HSP70 heat shock expression, with peak responses at 8 hours after a 2 hour exposure. The approximate threefold increase was substantially lower than that from thermal stimulation, raising questions about clinical utility.
Unknown authors · 2013
Unknown authors · 2013
Unknown authors · 2013
Researchers exposed cultured human fetal scleral fibroblasts to 0.2 mT ELF-EMF for 24 hours. The cells were treated with puerarin at concentrations of 0, 0.1, 1, and 10 microM. MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively after ELF-EMF exposure. Puerarin at 0.1 microM reversed the effect by 8.53% in mRNA and 17.97% in protein expression, with greater reversal at 1 and 10 microM.
Ketabi N, Mobasheri H, Faraji-Dana R. · 2013
Researchers investigated the effects of ultra high frequency nonionizing electromagnetic fields on the channel activities of the nanopore forming protein OmpF porin. Using the voltage clamp technique on an artificial bilayer, they exposed the pore to electromagnetic fields at 910 to 990 MHz and found that the frequency of channel gating and voltage sensitivity increased, while conductance remained unchanged. No changes in membrane capacitance or permeability were identified, and the energy transferred to nano-environments was estimated to address possible thermal effects.
