Outdoor EMF Infrastructure Research

Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure

Piszczek P et al. · 2022

Investigation of oxidative damage, antioxidant balance, DNA repair genes, and apoptosis due to radiofrequency-induced adaptive response in mice

Unknown authors · 2022

The study used 24 adult male Swiss-Albino mice divided into Four groups of 6. The radiofrequency and adaptive response groups were exposed to a 900 MHz GSM signal at 0.339 W/kg for 4 h/day over 7 days. After the final exposure, bleomycin was injected intraperitoneally at 37.5 mg/kg and the mice were sacrificed 30 minutes later. Oxidative damage and antioxidant mechanisms were examined in blood, DNA-repair gene expression was measured in liver, and apoptosis was assessed in liver using the TUNEL method. The adaptive response group showed increased ROS and p53 and OGG-1 expression, decreased SOD, CAT and TAC levels, and reduced apoptosis.

Effects of continuous prenatal and postnatal global system for mobile communications electromagnetic waves (GSM-EMW) exposure on the oxidative stress biomarkers in female rat liver

Unknown authors · 2022

Researchers continuously exposed pregnant Sprague-Dawley rats and their offspring to 900 MHz GSM-modulated RF-EMR for 24 hours per day and assessed liver oxidative stress at postnatal days 1, 9, and 21. Exposure significantly raised MDA levels at PND9 and PND21, lowered catalase activity at all ages, reduced catalase amount and mRNA at PND1 and PND9, and decreased GPx activity at PND21. CytoSOD levels increased at PND9 and 21 while mitoSOD levels decreased at PND21, and both GPx level and mitoSOD activity were elevated at all ages. SOD1 mRNA expression rose at PND1 but fell at PND9 and 21, whereas GPx1 expression was consistently reduced in the exposed group.

Investigation of oxidative damage, antioxidant balance, DNA repair genes, and apoptosis due to radiofrequency-induced adaptive response in mice

Unknown authors · 2022

Scientists exposed mice to 900 MHz cell phone radiation for 7 days, then administered a DNA-damaging drug called bleomycin. The RF-exposed mice showed increased DNA repair activity and reduced cell death compared to unexposed mice. This suggests low-level RF exposure may trigger protective cellular responses.

Effects of continuous prenatal and postnatal global system for mobile communications electromagnetic waves (GSM-EMW) exposure on the oxidative stress biomarkers in female rat liver

Salameh M et al. · 2022

Researchers exposed pregnant and newborn rats to 900 MHz radiofrequency radiation (the same frequency used by many cell phones) 24 hours per day and found significant liver damage in the offspring. The radiation caused oxidative stress, reduced protective antioxidant enzymes, and altered gene expression in the developing liver. This suggests continuous RF exposure during pregnancy and early development may harm liver function in offspring.

Effect of the prenatal electromagnetic field exposure on cochlear nucleus neurons and oligodendrocytes in rats

Unknown authors · 2022

Researchers randomly assigned pregnant Sprague-Dawley rats to a control group or a 900-MHz EMF exposure group, with the EMF group exposed daily until birth. Newborn rats were sacrificed by anesthesia on days 7, 10, 15, and 30. Structural damage in cochlear nuclear neurons and oligodendrocytes and increased caspase-3 activity were observed in the EMF groups compared to controls. However, no significant difference was found between groups in structural damage or caspase-3 activity across postnatal stages, and auditory brainstem response latencies did not differ. The authors concluded that prenatal 900-MHz EMF exposure has no harmful effects on ventral cochlear nucleus development.

Zinc affects nuclear factor kappa b and DNA methyltransferase activity in C3H cancer fibroblast cells induced by a 2100 MHz electromagnetic field

Unknown authors · 2022

EMF exposure increased cell viability compared with control, while zinc co-treatment reduced viability relative to EMF alone. EMF also significantly raised NF-κB and DNMT activities, and zinc supplementation significantly lowered these activities compared with EMF alone.

EMF promote BMSCs differentiation and functional recovery in hemiparkinsonian rats

Unknown authors · 2022

Researchers exposed purified BMSCs to sinusoidal or square waveform electromagnetic fields at 400 µT and 75 Hz for either 1 hour per week or 7 hours per day, then injected them into the left substantia nigra of Parkinsonian rats. Cell morphology analysis showed that sinusoidal EMF exposure caused most BMSCs to differentiate into neurons, including large and small neurons with apparent synapses. Square EMF exposure produced mainly astrocytes, microglia, and oligodendrocytes, while both BMSCs alone and sinusoidal EMF-exposed BMSCs improved locomotor activity in the rats.

Short-term exposure to radiofrequency radiation and metabolic enzymes' activities during pregnancy and prenatal development

Unknown authors · 2022

Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells

Unknown authors · 2022

The study aimed to examine the 96-hour effects of ELF-EMF on HERV-H, K, and W expression in human melanoma cells. SK-MEL-37 cells were continuously exposed to ELF-EMF at 50 Hz and intensities of 1.5 and 3 mT for 96 hours, and HERV expression was measured by qPCR after mRNA extraction. Exposure to ELF-EMF for 96 hours significantly downregulated HERV-H, K, and W env gene expression (P<0.001). The researchers suggested that low intensity and long-term exposure to ELF-EMF may pave using this type of radiation as a novel therapeutic approach by neutralizing the HERVs upregulated expression in melanoma cells.

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells

Unknown authors · 2021

Researchers isolated spermatogonial stem cells and Sertoli cells from neonate mouse testes. The stem cells were exposed to 50 Hz electromagnetic fields at 2.5 mT for one hour daily over five days and then treated with exosomes from pre-pubertal Sertoli cells. Electromagnetic field exposure increased reactive oxygen species, reduced catalase expression, cell viability, and colonization, and increased apoptosis and Caspase-3 expression while decreasing GFRα1 expression. Sertoli-derived exosomes significantly improved these adverse effects by regulating oxidative stress.

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells

Unknown authors · 2021

Researchers exposed mouse sperm stem cells to 50 Hz electromagnetic fields (like power lines) and found it caused oxidative damage and cell death. However, protective molecules called exosomes from neighboring Sertoli cells could reverse this damage. The study suggests EMF exposure harms male reproductive cells, but natural protective mechanisms exist.

Effects of Low-Intensity Microwave Radiation on Oxidant-Antioxidant Parameters and DNA Damage in the Liver of Rats

Unknown authors · 2021

Eighteen Sprague-Dawley male rats were divided into three groups of six each. Two groups were exposed to 1800 MHz or 2100 MHz microwave radiation for 2 hours per day over 7 months, while a third group served as sham control. After exposure, liver tissue was analyzed for DNA damage, malondialdehyde, 8-hydroxydeoxyguanosine, and total oxidant-antioxidant parameters. Both exposure frequencies significantly increased MDA, 8-OHdG, total oxidant status, oxidative stress index, and comet assay tail intensity, while decreasing total antioxidant status.

Effects of Low-Intensity Microwave Radiation on Oxidant-Antioxidant Parameters and DNA Damage in the Liver of Rats

Unknown authors · 2021

Researchers exposed rats to cell phone radiation at 1800 MHz and 2100 MHz frequencies for 2 hours daily over 7 months. Both frequencies caused significant liver damage, including DNA breaks and increased oxidative stress markers. The study demonstrates that even low-intensity microwave radiation can harm liver tissue through cellular damage mechanisms.

Hippocampal Oxidative Stress Induced by Radiofrequency Electromagnetic Radiation and the Neuroprotective Effects of Aerobic Exercise in Rats: A Randomized Control Trial

Unknown authors · 2021

Male Wistar rats were randomly assigned to 4 groups: moderate aerobic exercise, exposure to 900/1800 MHz radiofrequency electromagnetic waves for 3 hours per day, combined exercise and exposure, or exposure to an experimental phone without battery. Hippocampal dead cell count was significantly higher in the radiation-only group compared to the exercise, combined, and control groups. Malondialdehyde concentration was significantly higher in the radiation-only group than in the exercise, combined, and control groups. The activity of catalase, glutathione peroxidase, and superoxide dismutase was significantly higher in the exercise, combined, and control groups compared to the radiation-only group.

Hippocampal Oxidative Stress Induced by Radiofrequency Electromagnetic Radiation and the Neuroprotective Effects of Aerobic Exercise in Rats: A Randomized Control Trial

Unknown authors · 2021

Researchers exposed rats to cell phone radiation (900/1800 MHz) for 3 hours daily and found it caused brain cell death and oxidative damage in the hippocampus. However, rats that also performed moderate aerobic exercise showed protection against this radiation-induced brain damage, with significantly fewer dead brain cells and better antioxidant defenses.

Lost opportunities for cancer prevention: historical evidence on early warnings with emphasis on radiofrequency radiation

Hardell & Carlberg · 2021

The paper discusses historical lessons from early warnings on cancer risks and lost prevention opportunities. It states that radiofrequency radiation has a cancer-causing effect and that decades of studies show increased human cancer risk. Based on three case-control studies of wireless phone use, the authors present a preventable fraction for brain tumors and note an increase in undefined brain tumors in Sweden, especially among ages 20–39, which they suggest may be linked to high wireless phone use among young people.

Modulation of Macrophage Activity by Pulsed Electromagnetic Fields in the Context of Fracture Healing Bioengineering

Unknown authors · 2021

Researchers screened 22 ELF-PEMFs and identified two fields, A and B, with similar fundamental frequencies but different pulse patterns that diversely affected macrophage function. Macrophages exposed to field A showed pro-inflammatory markers such as increased phospho-Stat1, CD86, ROS, and pro-inflammatory cytokines. Macrophages exposed to field B showed anti-inflammatory and pro-healing markers including increased Arginase I, anti-inflammatory cytokines, and growth factors. Conditioned medium from field B-exposed macrophages enhanced stem cell migration more strongly than field A and stimulated extracellular matrix gene expression in stem cells.

Possible effects of different doses of 2.1 GHz electromagnetic radiation on learning, and hippocampal levels of cholinergic biomarkers in Wistar rats

Unknown authors · 2021

Researchers exposed rats to 2.1 GHz radiation at 45 V/m or 65 V/m for one week and compared them to a sham-exposed group. They tested memory using object location and Y-maze tasks. Rats exposed to 65 V/m performed worse on both memory tasks. The same rats had lower hippocampal levels of the cholinergic biomarkers AChE, ChAT, and VAChT.

Possible effects of different doses of 2.1 GHz electromagnetic radiation on learning, and hippocampal levels of cholinergic biomarkers in Wistar rats

Unknown authors · 2021

Researchers exposed rats to 2.1 GHz radiofrequency radiation at two different power levels for one week and tested their learning abilities. Rats exposed to the higher dose (65 V/m) showed impaired spatial memory and significantly reduced levels of key brain chemicals needed for learning and memory in the hippocampus. This suggests that even short-term exposure to this frequency can affect brain function in a dose-dependent manner.

Effects of 2.45 GHz Wi-Fi exposure on sleep-dependent memory consolidation

Unknown authors · 2021

Thirty young men participated in a double-blind, sham-controlled crossover study with all-night 2.45 GHz Wi-Fi exposure. Declarative memory improved overnight with Wi-Fi exposure, but emotional and procedural memory were unaffected. No sleep-specific physiological parameters changed with exposure, so the behavioral effect lacked physiological support and may be a random finding.

Protective effects of selenium on electromagnetic field-induced apoptosis, aromatase P450 activity, and leptin receptor expression in rat testis

Khoshbakht S et al. · 2021

Twenty-four male Wistar rats were divided into four groups: CONT (control), EM group (2100 MHZ), EM/SE group (2100 MHZ + selenium (0.2 mg/kg)), and SE group (selenium 0.2 mg/kg). EMF exposure significantly reduced serum LH, FSH, GnRH, testosterone, sperm count, germinal epithelium thickness, and seminiferous tubule diameter, while increasing serum leptin, sperm abnormality, aromatase, apoptotic cells, and leptin receptor. Selenium co-administration increased sperm count, germinal epithelium thickness, seminiferous diameters, and hormone levels, and decreased sperm abnormality, leptin receptor, and apoptotic cells compared with EMF alone.

Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line

Zeni O, Romeo S, Sannino A, Palumbo R, Scarfì MR · 2021

Researchers exposed SH-SY5Y neuroblastoma cells to 1950 MHz radiofrequency fields. Medium from the exposed cells reduced menadione-induced DNA damage in recipient non-exposed cells (P<0.05, comet assay). Direct exposure to the same RF conditions also reduced DNA damage (P<0.05) and increased extracellular hsp70 without changing intracellular hsp70.

900 MHz Radiofrequency Field Induces Mitochondrial Unfolded Protein Response in Mouse Bone Marrow Stem Cells

Wen X et al. · 2021

Researchers exposed mouse bone marrow stromal cells to continuous wave 900 MHz radiofrequency fields at 120 μW/cm2 for 4 hours per day over 5 consecutive days. ROS levels increased significantly at 30 minutes and 4 hours after exposure but returned to baseline by 24 hours. Expression of HSP10, HSP60, and ClpP proteins increased significantly at 30 minutes and 4 hours but reversed by 24 hours. When JNK2 was inhibited with siRNA, the RF exposure no longer increased these protein levels.

900 MHz Radiofrequency Field Induces Mitochondrial Unfolded Protein Response in Mouse Bone Marrow Stem Cells

Xie W et al. · 2021

Chinese researchers exposed mouse bone marrow stem cells to 900 MHz radiofrequency radiation (the same frequency used by many cell phones) for 4 hours daily over 5 days. The radiation triggered a cellular stress response in the mitochondria (the cell's powerhouses), causing them to produce stress proteins and reactive oxygen species, though cells appeared to recover within 24 hours.