Unknown authors · 2000
Researchers investigated whether exposure to 60-Hz sinusoidal magnetic fields (0.3-1.2 G for 3-72 h) would cause proliferation of human astrocytoma cells. Sixty-Hertz magnetic fields caused a time- and dose-dependent increase in proliferation of astrocytoma cells, measured by (3)H-thymidine incorporation and by flow cytometry, and strongly potentiated the effect of two agonists. However, magnetic fields had no effect on DNA synthesis of rat cortical astrocytes, which are similar nontransformed cells. A protein kinase C inhibitor and down-regulation of protein kinase C inhibited the effect of magnetic fields on basal and agonist-stimulated thymidine incorporation.
Unknown authors · 2000
Researchers examined the effect of 50 mT, 60 Hz ELFMF on heat-induced HSP-70 expression in HL60RG cells. Exposure to 50 mT ELFMF alone did not increase HSP-70 production. Combined exposure to 50 mT ELFMF and mild heat at 40°C and 42°C suppressed heat-induced HSP-70 expression. This suppression required simultaneous exposure for more than 5 hours. Lower magnetic densities of 5 mT and 0.5 mT did not suppress HSP-70.
Unknown authors · 2000
Laurence JA, French PW, Lindner RA, Mckenzie DR · 2000
The aim was to investigate mechanisms for the effects of pulsed microwave radiation on protein conformation. The researchers modeled heat diffusion and showed that pulsed exposure can cause transient temperature excursions outside the normal range even when athermal. They propose that the power window phenomenon may be caused by incomplete triggering of the heat shock response.
Kalns J, Ryan KL, Mason PA, Bruno JG, Gooden R, Kiel JL. · 2000
Grant FH, Schlegel RE, · 2000
Researchers evaluated 20 pacemaker-phone combinations involving 6 pacemakers and 9 phones in vitro under worst-case conditions. The phones represented CDMA, TDMA-11 Hz, TDMA-22 Hz, TDMA-50 Hz, and TDMA-217 Hz digital wireless technologies. Small increases in the perpendicular air gap between the phone and the saline surface resulted in a dramatic reduction in interaction, with approximately half of the 208 test runs showing interaction at 1 cm no longer interacting at 2 cm. At a gap of 7.4 cm the percentage of runs with interaction decreased to 1.4%, and the overall interaction rate was less than 0.07% at a perpendicular distance of 8.6 cm.
Gapeev AB, Chemeris NK · 2000
Researchers used a mathematical model to analyze how modulated extremely high frequency electromagnetic radiation affects intracellular calcium concentration in neutrophils. The model showed that the calcium rise depends on both the frequency and phase of the modulating signal relative to chemical stimulation. A rise above 50% of the initial calcium level occurred at about 1 Hz and phase range 0.3-2.5 radians, but only when modulating signal intensity exceeded a threshold and chemical stimulus intensity was high. Added noise changed the phase-frequency characteristics and created multiple response bands with higher Q-factors. The model results matched earlier experimental data showing effects only with extracellular calcium and high calcium ionophore A23187 concentrations.
Chiabrera A, Bianco B, Moggia E, Kaufman JJ, · 2000
The aim of the study was to observe Zeeman-Stark modeling of the RF EMF interaction with ligand binding. The model accounts for ion collision losses, endogenous forces, metabolic non-equilibrium, and thermal noise. The model predicts that ligand binding probability may be affected by low-intensity exogenous EMF when the receptor's potential energy well matches the radiofrequency photon energy. The authors state that the results point toward electromagnetic control of biochemical processes and the need for a new database of safety standards.
Romano-Spica V, Mucci N, Ursini CL, Ianni A, Bhat NK · 2000
Researchers analyzed gene expression in hemopoietic and testicular cell types after exposure to 50 MHz radiofrequency radiation modulated at 16 Hz. They observed overexpression of ets1 mRNA in Jurkat T-lymphoblastoid and Leydig TM3 cell lines only when the 16 Hz modulation was present. A putative candidate gene was also repressed after EMF exposure.
Pashovkina MS, Akoev IG · 2000
Researchers studied the activity of alkaline phosphatase during exposure to pulse-modulated microwave radiation. The carrier frequency was 2375 MHz with modulation pulse rates of 10-390 Hz, on-off time ratio 2, and specific absorption rates of 8 and 0.8 microW/cm2. At a modulation frequency of 70 Hz, alkaline phosphatase activity increased 1.8-2.0 times. Exposure times were 1 and 3 minutes under continuous temperature control.
Miyakoshi J, Koji Y, Wakasa T, Takebe H · 1999
Researchers exposed Chinese hamster ovary K1 cells to 5 mT ELFMF at 60 Hz for up to 6 weeks with or without X-irradiation. Long-term exposure to 5 mT ELFMF alone did not increase mutant frequency of the HPRT gene. However, exposure to 5 mT ELFMF after X-irradiation enhanced the X-ray-induced mutation rate. As little as 1 week of ELFMF exposure after X-irradiation increased the mutation rate.
Unknown authors · 1999
Researchers evaluated the effect of a 60 Hz electromagnetic field on NF-kappaB or AP-1-dependent reporter gene expression in human U937 leukemia cells. Reporter genes were electroporated into the cells and pathway activation was measured by CAT protein using CAT ELISA. Magnetic field intensities of 0.08, 0.1, 1.0, and 1.3 mT had no effect on either signaling pathway.
Unknown authors · 1999
Unknown authors · 1999
Researchers investigated the effect of 50 Hz magnetic fields on the cytosolic calcium oscillator in Jurkat E6.1 cells at field strengths from 0 to 0.40 mT root mean square. The total spectral power of the cytosolic Ca2+ oscillator was reduced by magnetic field exposure and the effect increased in a dose-response manner. This reduction was also observed in the 0-10 mHz subinterval of the oscillation spectrum, while higher frequency subintervals showed no significant change.
Unknown authors · 1999
Researchers exposed HBL-100 cells and normal human mammary epithelial cells to 60 Hz EMF at flux densities of 0.1, 1.0, and 10.0 Gauss for periods ranging from 20 min to 24 h. Gene expression was quantitated using ribonuclease protection assays. EMF exposure had no statistically significant effect on basal levels of c-myc transcripts in either human breast cell model, and had no effect on alterations in c-myc expression induced by 12-O-tetradecanoylphorbol-13-acetate. Transcript levels of c-erbB-2, p53, p21, GADD45, bax, bcl-x, mcl-1, and c-fos were also unaffected by EMF exposure.
Unknown authors · 1999
Researchers exposed HL-60 cells to a sinusoidal 0.1 or 1 mT magnetic field at 60 Hz for 2 hours and then pulse-labeled them with radioactive isotopes. In control cultures without magnetic field exposure, the cognate form of stress protein 72c was faintly observed, and 0.1 mT exposure did not alter stress protein metabolism compared to controls. At 1 mT, the inducible form of stress protein 70i was synthesized, stress protein 90 was phosphorylated but not synthesized, and stress protein 27 isoforms a and b were induced. Statistical analysis of Western blot data confirmed a significant increase in stress proteins 90 and 27a after 1 mT exposure.
Velizarov, S, Raskmark, P, Kwee, S, · 1999
Researchers exposed cell cultures to a GSM 960 MHz field in a TEM cell at 39°C and 35°C. Exposed cells showed a significant change in proliferation compared to non-exposed controls at both temperatures. Sham-exposed cells showed no significant change in proliferation at either temperature.
Unknown authors · 1998
Researchers exposed HL60 cells to 6-microT 60 Hz magnetic fields and measured MYC expression. The assay methods and exposure conditions were as close as practical to those of the investigators that originally reported a positive effect. A chemical agent demonstrated the cells responded to a known stimulus and the system had low basal variability to detect effects of the previously reported magnitude. Using cells from a commercial source and from the original investigators, no evidence was found that EMF exposure induces MYC expression.
Unknown authors · 1998
Researchers exposed cultured human osteosarcoma Saos-LP-12 cells to a 400 mT, 50 Hz extremely low frequency magnetic field. The exposure induced mutations in the HPRT gene. The Saos-LP-12 cells have a deletion in the p53 coding region. Introducing a wild-type p53 expression plasmid into these cells suppressed the ELFMF-induced mutations. No marked difference in the mutation spectrum was observed among ELFMF exposure with or without p53 and sham exposures.
Unknown authors · 1998
Unknown authors · 1998
Researchers screened 1,920 randomly selected cDNAs in HL60 cells exposed to 60 Hz magnetic fields (2 mT) or X rays (5 Gy) compared to unexposed cells. Eighteen cDNAs were differentially expressed in X-irradiated cells, and 15 matched known genes related to cell growth, protein synthesis, energy metabolism, oxidative stress, or apoptosis. BIGEL analysis revealed no differences greater than 1.5-fold in expression levels in magnetic-field-exposed cells compared to sham-exposed cells.
Unknown authors · 1998
Researchers exposed DT40 lymphoma B cells to low energy electromagnetic fields and measured inositol phospholipid turnover. PLC-gamma2-deficient cells and cells with SH2 domain mutant PLC-gamma2 did not show increased IP3 levels after EMF exposure. SYK-deficient cells lacked PLC-gamma2 activation, and the C-terminal SH2 domain of SYK was essential. LYN-deficient cells did not show enhanced inositol phospholipid turnover, but introduction of wild-type fyn restored responsiveness. The results indicate EMF-induced PLC-gamma2 activation is mediated by LYN-regulated stimulation of SYK downstream of LYN and upstream of PLC-gamma2.
Unknown authors · 1998
Researchers exposed DT40 lymphoma B-cells to low energy electromagnetic fields and observed activation of PLC-γ2 and increased inositol phospholipid turnover. PLC-γ2 activation was mediated by stimulation of Bruton's tyrosine kinase (BTK), which acts downstream of LYN kinase and upstream of PLC-γ2. BTK-deficient B-cells did not show enhanced PLC-γ2 activation in response to EMF, but introducing the wild-type human btk gene restored EMF responsiveness. The authors conclude that BTK exerts a pivotal and mandatory function in initiation of EMF-induced signaling cascades in B-cells.
Unknown authors · 1998
Researchers used BTK-deficient DT40 B cells reconstituted with the human btk gene to test whether a 1-gauss 60-Hz electromagnetic field alters inositol 1,4,5-trisphosphate production. Blinded exposures with anti-IgM treatment as a positive control showed no significant effect of the field on inositol 1,4,5-trisphosphate production. The researchers also evaluated BTK activation in wild-type DT40 cells and found that while pervanadate activated BTK in a dose- and time-dependent manner, detection of BTK activation depended on the kinase buffer. Using cells from the original investigators, no evidence supported the hypothesis that the magnetic field had a causal effect on protein-tyrosine kinase activities affecting inositol 1,4,5-trisphosphate production.
Unknown authors · 1998
Researchers examined the separate and combined effects of 60 Hz magnetic fields and a phorbol ester on PKC activity in HL60 cells. Exposure to a 1.1 mT magnetic field alone or combined with 2 microM PMA for 1 h did not enhance PKC activity. In a second experiment, cells preexposed to 50 nM PMA for 45 min then exposed to both PMA and magnetic field for 15 min showed a greater decrease in cytosolic PKC activity and a larger increase in membrane activity than PMA treatment alone or PMA with sham exposure. The authors concluded that magnetic fields may act synergistically on an already activated pathway.
