Shahin S et al. · 2013
Twelve-week-old female mice were exposed to 2.45 GHz microwave radiation for 2 hours per day for 45 days to study effects on implantation and pregnancy. Implantation sites were significantly affected in the microwave-irradiated mice compared to controls. The researchers concluded that low-level microwave irradiation-induced oxidative stress suppresses implantation and may also lead to deformity of the embryo if pregnancy continues.
Liu C, Duan W, Xu S, Chen C, He M, Zhang L, Yu Z, Zhou Z. · 2013
Researchers exposed a mouse spermatocyte-derived GC-2 cell line to 1800 MHz GSM signals for 24 hours using an intermittent schedule of 5 minutes on and 10 minutes off, at SAR levels of 1 W/kg, 2 W/kg, or 4 W/kg. At a SAR of 4 W/kg, DNA migration in a modified comet assay was significantly increased, and levels of the DNA adduct 8-oxoguanine (8-oxoG) were also increased. No detectable DNA strand breakage was observed by the alkaline comet assay. The increases in DNA damage were accompanied by increases in reactive oxygen species (ROS), and these effects were mitigated by co-treatment with the antioxidant alpha-tocopherol.
Hancı H et al. · 2013
Researchers exposed pregnant rats to 900-MHz EMF during days 13–21 of pregnancy and examined the testicles of their offspring at postnatal day 21. The offspring of exposed mothers showed irregularities in seminiferous tubule basal membrane and epithelium, immature germ cells in the lumen, and a decreased diameter in seminiferous tubules and thickness of epithelium. Apoptotic index, lipid peroxidation, and DNA oxidation were higher in the offspring of exposed mothers than in controls.
Bilgici B, Akar A, Avci B, Tuncel OK · 2013
Researchers exposed rats to a 900 MHz electromagnetic field at a whole body average specific absorption rate of 1.08 W/kg for 1 hour per day for 3 weeks. Malondialdehyde and advanced oxidation protein product levels increased in rat brain tissue after electromagnetic field exposure. Nitric oxide levels in the brain showed no significant difference, but serum nitric oxide increased significantly after exposure, and garlic did not reduce that increase. The researchers also tested orally administered garlic powder at 500 mg/kg/day and found it had a protective effect against the oxidative stress seen in brain tissue.
Atasoy HI, Gunal MY, Atasoy P, Elgun S, Bugdayci G · 2013
Researchers exposed male rats to radiofrequency radiation from Wi-Fi devices operating at 2.437 GHz for 24 hours a day for 20 weeks, then examined their testes. The exposed rats showed significant increases in markers indicating DNA damage in the testes compared to controls. Catalase and glutathione peroxidase activity were decreased in the exposed group, which the researchers suggested may have been due to radiofrequency effects on enzyme activity. The researchers said these findings raise questions about the safety of radiofrequency exposure from Wi-Fi Internet access devices for growing organisms of reproductive age, with a potential effect on both fertility and the integrity of germ cells.
Kurzeja E et al. · 2013
Researchers studied the potential relationship between static magnetic field exposure and the antioxidant system of fibroblasts cultured with fluoride ions. Three chambers with static magnetic fields of different intensities (0.4, 0.6, and 0.7 T) were used, and fluoride ions were added at a concentration of 0.12 mM. Static magnetic fields were found to reduce the oxidative stress caused by fluoride ions and to normalize the activities of antioxidant enzymes including superoxide dismutase, glutathione peroxidase, and catalase. The results also suggest that exposure to fluoride and a static magnetic field improves the tolerance of cells to the oxidative stress induced by fluoride ions.
Glinka M, Sieroń A, Birkner E, Cieślar G · 2013
Researchers measured the activity of antioxidant enzymes and a lipid peroxidation marker in male rats exposed to an extremely low-frequency magnetic field during skin wound healing. Each animal had an experimental cut wound on its back. The 40 Hz, 10 mT exposure was associated with increased glutathione peroxidase activity in serum and decreased malone dialdehyde in liver tissue, which the researchers described as suggesting inhibition of phospholipid peroxidation and stabilization of cellular membranes.
Wang X et al. · 2013
Researchers exposed early adolescent male mice to a 50 Hz magnetic field at 2 mT for 60 minutes per day from postnatal day 23 to 35. Spatial memory was then tested using the Y-maze and Morris water maze tasks. The magnetic field exposure did not affect Y-maze performance but improved spatial learning acquisition and memory retention in the water maze.
Kitaoka K, Kitamura M, Aoi S, Shimizu N, Yoshizaki K. · 2013
Researchers investigated whether chronic extremely low-frequency magnetic field exposure affected emotional behavior and corticosterone synthesis in mice, using an intensity of 3 mT over a total exposure of 200 hours. Exposed mice showed a significant increase in total immobility time in a forced swim test and showed latency to enter the light box in a light-dark transition test, compared with sham-treated mice. Corticosterone secretion was significantly high in the exposed mice, but no changes were observed in adrenocorticotropic hormone or stress-response gene expression, and Cyp17a1 mRNA was significantly reduced.
He YL, Liu DD, Fang YJ, Zhan XQ, Yao JJ, Mei YA. · 2013
Researchers investigated the effects of extremely low-frequency electromagnetic fields (ELF-EMF) on sodium channel activity in rat cerebellar granule cells. Exposing the cells to ELF-EMF for 10–60 minutes significantly increased sodium currents by 30–125% in a time- and intensity-dependent manner. Increases in intracellular arachidonic acid, prostaglandin E2, and phosphorylated PKA levels were observed in the cells following ELF-EMF exposure. The data demonstrated for the first time that neuronal sodium currents are significantly increased by ELF-EMF exposure via a cPLA2, arachidonic acid, prostaglandin E2, EP receptors, and PKA signaling pathway.
Gutiérrez-Mercado YK et al. · 2013
Researchers used 96 Wistar male rats divided into three groups of 32 rats each: a control group, a sham group, and an experimental group exposed to extremely low frequency magnetic fields at 120 Hz and 0.66 mT. All animals received colloidal carbon intravenously, and capillary permeability was studied using optical and transmission electron microscopy. In the exposed group, researchers detected an increase in capillary permeability and a significant increase in vascular area, effects not seen in the control or sham groups.
El Gohary MI, Salama AA, El Saeid AA, El Sayed TM, Kotb HS. · 2013
Researchers studied how caffeine and extremely low-frequency magnetic field (ELF-MF) exposure affected brain electrical activity in rats over 15 days. Caffeine given at 10 mg/kg caused a reduction in the mean power amplitude of EEG signals across most frequency bands, especially the alpha band (8–12 Hz), and this effect was more evident in motor cortex than in visual cortex. Exposure to ELF-MF at 0.2 mT enhanced mean power amplitude in most EEG frequency bands, with the effect more pronounced in the right hemisphere than the left. When caffeine was given alongside ELF-MF exposure, a large increase in alpha-band amplitude appeared after 5 days at all recording sites.
Duan Y, Wang Z, Zhang H, He Y, Lu R, Zhang R, Sun G, Sun X. · 2013
Researchers investigated whether lotus seedpod procyanidins (LSPCs) given by oral gavage could protect mice from cognitive deficits and oxidative damage caused by extremely low frequency electromagnetic field (ELF-EMF) exposure at 50 Hz and 8 mT for 28 days. A water maze test indicated that learning and memory abilities of the ELF-EMF group deteriorated significantly compared with the control group, but the ELF-EMF + LSPCs90 group had remarkably improved learning and memory abilities compared with the ELF-EMF group. ELF-EMF exposure was associated with significant increases in oxidative stress markers and decreases in antioxidant enzyme activities.
Deng Y, Zhang Y, Jia S, Liu J, Liu Y, Xu W, Liu L. · 2013
Researchers divided sixty male SPF Kunming mice into four groups: a control group, an ELF-MF group exposed at 2 mT for 4 hours per day, a group given aluminum at 200 mg per kg, and a combined ELF-MF plus aluminum group. After 8 weeks of treatment, all three exposure groups showed learning memory impairment, with prolonged escaping latency to the platform and a reduced percentage of time in the platform quadrant in the Morris water maze task. All three exposed groups showed a statistically significant decrease in superoxide dismutase activity and an increase in malondialdehyde levels. The combined ELF-MF plus aluminum treatment induced no more damage than ELF-MF and aluminum did separately.
Balassa T et al. · 2013
Researchers exposed rats to extremely low-frequency magnetic fields (0.5 and 3 mT, 50 Hz) during two critical periods of brain development: in utero during the second gestation week, or as newborns for 7 days starting 3 days after birth. Basic excitability of hippocampal slices was increased by both the fetal 0.5 mT and the newborn 3 mT treatments. Fetal exposure significantly inhibited paired-pulse depression and significantly decreased the efficacy of long-term potentiation induction in the neocortex, but not in the hippocampus. Neonatal treatment had no significant effect on plasticity phenomena.
Bai WF, Xu WC, Feng Y, Huang H, Li XP, Deng CY, Zhang MS. · 2013
Researchers divided rat bone marrow stromal cells into three groups: one treated with electromagnetic fields, one induced without fields, and a control group. The cells in the EMF group expressed neuron-specific markers and genes, formed synaptic junctions, and pulsed excitatory postsynaptic currents. The researchers suggested that EMF appears to be a promising clinical option for stem cell transplantation therapies to combat central nervous system diseases.
Akdag MZ, Dasdag S, Cakir DU, Yokus B, Kizil G, Kizil M. · 2013
Researchers exposed adult male Sprague-Dawley rats to 50 Hz ELF magnetic fields at 100 or 500 μT for 2 hours per day for 10 months, then measured beta-amyloid protein, protein carbonyl, and malondialdehyde in the brain. Protein carbonyl and malondialdehyde levels were increased by exposure to both field strengths, while beta-amyloid protein levels were not significantly changed.
Wang H et al. · 2013
Researchers exposed 100 Wistar rats to a 2.856 GHz pulsed microwave field at average power densities of 0, 5, 10, and 50 mW/cm² for 6 minutes. Rats exposed to 10 or 50 mW/cm² showed significant deficits in spatial learning and memory at 6 hours, 1 day, and 3 days after exposure. At the 5 mW/cm² level, exposed rats showed no difference compared with the sham group. The study suggested that impairment of LTP induction and damages of hippocampal structure, especially changes of synapses, might contribute to cognitive impairment after microwave exposure.
Tong J, Chen S, Liu XM, Hao DM · 2013
Researchers recorded hippocampal CA1 neuron activity in rats exposed to 900 MHz electromagnetic radiation at 10 W/m2 using glass electrode extracellular recording and a polygraph. Discharge frequency of hippocampal CA1 neurons was reduced after exposure, though the change in scale was not obvious. Electromagnetic radiation might change the spontaneous discharge patterns of hippocampus CA1 region neurons, making the explosive discharge pattern increase obviously. The researchers suggested that these changes may lead to a decrease in the ability of learning and memory.
Sharma A, Sisodia R, Bhatnagar D, Saxena VK · 2013
Researchers exposed Swiss albino mice to 10 GHz microwaves at a power density of 0.25 mW/cm2 for 2 hours per day for 30 days, then tested spatial memory using the Morris water maze test. Exposed mice showed statistically significant higher mean latency to reach the target quadrant compared to sham-exposed mice. A decrease in protein levels was also estimated in the whole brain of exposed mice compared to sham-exposed mice.
Pelletier A et al. · 2013
Researchers examined the effects of chronic RF-EMF exposure (900 MHz, 1 V m(-1)) on the main functions involved in body energy homeostasis, including feeding behaviour, sleep, and thermoregulatory processes. Thirteen juvenile male Wistar rats were exposed to continuous RF-EMF for 5 weeks and compared with 11 non-exposed animals. The frequency of rapid eye movement sleep episodes was greater in the exposed group independently of ambient temperature, with increases of 42.1% at 24 °C and 31.6% at 31 °C. Exposure also increased daytime food intake, and the researchers concluded that RF-EMF exposure can induce energy-saving processes without strongly disturbing the overall sleep pattern.
Odacı E et al. · 2013
Researchers exposed pregnant rats to 900 MHz electromagnetic fields for one hour daily between days 13 and 21 of pregnancy, then assessed the female pups' spinal cord tissue and motor behavior. The rotarod test showed a significant increase in motor functions in the EMF group pups (p=0.037), while the open field test found no difference between groups. Pathological changes were observed in the spinal cords of the EMF group pups, and the researchers suggested these changes may have led to the increase in motor activity.
Ntzouni MP et al. · 2013
Narayanan SN et al. · 2013
Researchers exposed male adolescent rats to radio-frequency electromagnetic radiation at 900 MHz from an active mobile phone, then tested their emotionality and locomotion. In the elevated plus maze test, exposed rats showed significantly reduced open-arm entries, time on open arms, and distance on open arms, along with decreased rearing and grooming frequency. Total distance travelled and total arm entries did not differ significantly between exposed and control rats.
Hao D, Yang L, Chen S, Tong J, Tian Y, Su B, Wu S, Zeng Y · 2013
Researchers exposed 32 trained Wistar rats to 916 MHz, 10w/m2 electromagnetic field for 6 hours a day, 5 days a week, over 10 weeks, and tested their spatial learning and memory in an eight-arm radial maze each weekend. During weeks 4–5, the exposed rats had longer completion times and higher error rates than the control group, but during weeks 1–3 and 6–9 the two groups were close to each other. The researchers concluded that the 916 MHz EMF influenced learning and memory in rats to some extent in a period during exposure, and that the rats can adapt to long-term EMF exposure.
