Yang G, Ren Z, Mei YA. · 2015
Researchers studied the effects of 50 Hz magnetic fields on GABAA receptor activity in rat cerebellar granule neurons. Exposing the neurons to 1 mT of the field for 60 minutes significantly increased GABAA receptor currents without modifying sensitivity to GABA. Levels of phosphorylated PKC were significantly elevated after 60 minutes of exposure, and this elevation was blocked by an EP1 receptor-specific antagonist.
Zhao QR, Lu JM, Yao JJ, Zhang ZY, Ling C, Mei YA. · 2015
Researchers used a mouse model to examine the effects of extremely low-frequency (50 Hz) electromagnetic field exposure on a recognition memory task and morphological changes of hippocampal neurons. Exposure at 1 mT for 12 hours per day produced a time-dependent deficit in novel object associative recognition memory and decreased hippocampal dendritic spine density. The memory deficit was transient, observed only after exposing mice to the fields for 7 to 10 days, and occurred without changes in spontaneous locomotor activity. Over-expression of neuritin in the hippocampus using an adeno-associated virus vector significantly increased neuritin levels and dendritic spine density, and neuritin prevented the exposure-induced effect by increasing hippocampal spine density.
Duan W et al. · 2015
Researchers exposed a mouse spermatocyte-derived GC-2 cell line intermittently to 50 Hz ELF-EMF at intensities of 1, 2, or 3 mT, or to 1800 MHz RF-EMF in GSM-Talk mode at SAR values of 1, 2, or 4 W/kg. After 24 hours of exposure, neither ELF-EMF nor RF-EMF affected cell viability. ELF-EMF exposure significantly increased DNA strand breaks at 3 mT, while RF-EMF exposure significantly induced oxidative DNA base damage at a SAR of 4 W/kg. The researchers suggest that both field types may produce genotoxicity at relatively high intensities but through different patterns of DNA damage.
Giorgi G et al. · 2014
Researchers investigated whether pulsed magnetic field exposure could interfere with DNA damage and repair in human neuroblastoma BE(2)C cells under oxidative stress. Cells were treated with hydrogen peroxide and exposed to a pulsed magnetic field at 1 mT and 50 Hz, and DNA double strand breaks were tracked using gamma-H2AX foci formation at 1, 24, 48, and 72 hours after treatment. The results suggest that pulsed magnetic field exposure does not interfere with genotoxicity and cytotoxicity induced by oxidative stress.
Salunke BP, Umathe SN, Chavan JG. · 2014
Researchers exposed mice to 50 Hz magnetic fields from power lines for 8 hours daily up to 120 days. The mice developed obsessive-compulsive behaviors and showed increased nitric oxide levels in brain regions controlling behavior, suggesting power-frequency fields can alter brain chemistry.
Salunke BP, Umathe SN, Chavan JG. · 2014
Researchers exposed Swiss albino mice to a 50 Hz, 10 G extremely low-frequency magnetic field for 8 hours per day across periods of up to 120 days. Anxiety was measured using an elevated plus maze, open field test, and social interaction test, on the 7th, 30th, 60th, 90th, and 120th exposure day, respectively. The findings suggest that the magnetic field significantly induced anxiety behavior and that the NMDA receptor was involved in its effect. Glutamate and GABA levels were significantly elevated in the hippocampus and hypothalamus, though GABA receptor modulators did not produce a significant effect on the induced anxiety.
Reale M et al. · 2014
Researchers exposed human brain cells to 50 Hz magnetic fields from household electricity and found they triggered harmful oxidative stress. The cells' natural defenses initially compensated, but failed when combined with other stressors, suggesting everyday EMF exposure may increase brain vulnerability to damage.
Li Y, Yan X, Liu J, Li L, Hu X, Sun H, Tian J. · 2014
Researchers exposed newborn rat nerve cells to 50 Hz electromagnetic fields (power line frequency) for two hours. The exposure increased production of BDNF, a protein essential for nerve growth and brain health, by triggering calcium flow into cells and activating specific cellular pathways.
Reale M et al. · 2014
Scientists exposed human brain cells to 50 Hz electromagnetic fields from power lines for 24 hours. The EMF exposure caused cellular damage and weakened the cells' natural defense systems, especially when cells were already stressed, suggesting potential links to brain degeneration.
de Groot MW, Kock MD, Westerink RH. · 2014
Researchers exposed nerve cells (PC12 cells) to 50 Hz magnetic fields at extremely high levels - up to 1000 microteslas, which is 10,000 times stronger than typical background exposure. They tested both healthy cells and chemically-stressed cells that were more vulnerable to damage. The study found no toxic effects on the nerve cells' calcium balance, oxidative stress levels, or cell membrane integrity, even at these extraordinarily high exposure levels.
Jin YB et al. · 2014
Researchers assessed DNA damage using comet assays in mouse and human cell lines exposed to 60-Hz, 1 mT ELF-MF for 4 or 16 hours. Cells exposed to ELF-MF alone showed no significant DNA damage compared to unexposed cells. No synergistic or additive DNA damage occurred when ELF-MF was combined with ionizing radiation, hydrogen peroxide, or c-Myc activation.
de Groot MW, Kock MD, Westerink RH. · 2014
Researchers investigated acute (30 min) and sub-chronic (48 h) exposure to 50 Hz ELF-EMF on naïve and chemically stressed PC12 cells. Acute exposure up to 1000 μT did not affect basal or depolarization-evoked calcium levels in naïve cells. Sub-chronic exposure up to 1000 μT had no consistent effects on calcium homeostasis, ROS production, or membrane integrity in naïve cells. In chemically stressed cells, both acute and sub-chronic exposure also failed to exert consistent effects on calcium homeostasis, ROS production, and membrane integrity. The authors concluded that exposure up to 1000 μT does not induce neurotoxic effects in vitro in either cell type.
Alsaeed I et al. · 2014
Researchers examined the effects of perinatal exposure to ELF EMF on autism-relevant behavioral parameters in male BALB/C mice. Exposed mice lacked normal sociability and preference for social novelty, and also showed decreased exploratory activity. The researchers concluded that the results are supportive of the hypothesis of a causal link between exposure to ELF EMF and ASD, but recommended replications with further tests.
Salunke BP, Umathe SN, Chavan JG · 2014
Researchers exposed mice to 50 Hz magnetic fields (power line frequency) for 8 hours daily up to 120 days. This caused obsessive-compulsive behaviors by increasing nitric oxide levels in brain regions. The study suggests household electrical frequencies may affect brain chemistry and behavior.
Salunke BP, Umathe SN, Chavan JG · 2014
Researchers exposed Swiss albino mice to an extremely low frequency magnetic field (50 Hz, 10 G) for 8 hours per day for up to 120 days to study obsessive-compulsive disorder-like behavior. OCD-like behavior was measured using the marble burying behavior test, and the results showed that the magnetic field induced time-dependent marble burying behavior on each exposure day tested. A nitric oxide precursor worsened the OCD-like behavior, while nitric oxide synthase inhibitors reduced it, with corresponding changes in nitric oxide levels.
Reale M et al. · 2014
Researchers evaluated the effects of an electromagnetic wave at a magnetic field intensity of 1 mT and a frequency of 50-Hz on human SH-SY5Y cells, a well-characterized immortalized neuronal cell model. ELF-EMF exposure elevated the expression of NOS and O2−, which were countered by compensatory changes in antioxidant catalase activity and enzymatic kinetic parameters related to CYP-450 and CAT activity. Cytokine gene expression was additionally evaluated and found rapidly modified by ELF-EMF exposure. When co-exposed to H2O2-induced oxidative stress, ELF-EMF proved not as well counteracted, resulting in a decline in CAT activity and a rise in O2− levels.
Jung IS, Kim HJ, Noh R, Kim SC, Kim CW. · 2014
Researchers investigated how extremely low frequency magnetic fields affect nerve-growth-factor-induced neuronal differentiation of PC12 cells using proteomic methods. After 5 days of exposure at 50 Hz and 1 mT, neurite length and the number of neurite-bearing cells increased, while proliferation activity decreased. Six proteins were found to be differentially expressed between exposed and unexposed NGF-treated cells, including peripherin, VGF8a, HSP72-psI, LMW-PTP/ACP1, TUBA1A, and OPA1L.
Li L, Xiong DF, Liu JW, Li ZX, Zeng GC, Li HL. · 2013
Researchers tested cognitive and brain function in 310 Chinese electrical workers regularly exposed to power line electromagnetic fields during equipment inspections, comparing them to 300 unexposed office workers. The study found no differences in memory, reaction time, or other brain performance measures between the two groups. This suggests that occupational exposure to power frequency electromagnetic fields may not impair basic cognitive abilities.
Park JE, Seo YK, Yoon HH, Kim CW, Park JK, Jeon S · 2013
Researchers treated human bone marrow derived mesenchymal stem cells (hBM-MSCs) with ELF-EMF at 50 Hz and 1 mT and measured neural marker expression and cell signaling. The expression of neural markers NF-L, MAP2, and NeuroD1 increased at 6 days, while phosphorylation of Akt and CREB, but not ERK, increased at 90 min. The researchers suggest that EMF induces neural differentiation through activation of EGFR signaling and mild generation of reactive oxygen species (ROS).
Jin YB et al. · 2012
Researchers exposed mouse NIH3T3 and human WI-38 fibroblast cells to a 60 Hz, 1 mT magnetic field for 4 hours, with or without ionizing radiation, hydrogen peroxide, or c-Myc activation. Cells exposed to the magnetic field alone showed no significant difference in micronucleus frequency compared to unexposed cells. No synergistic effects were observed when the magnetic field was combined with ionizing radiation, hydrogen peroxide, or c-Myc activation.
Cui Y, Ge Z, Rizak JD, Zhai C, Zhou Z, Gong S, Che Y. · 2012
Researchers examined the effects of extremely low frequency magnetic field (ELF-MF) exposure on learning in mice using two water maze tasks. ELF-MF exposure at 1 mT, 50 Hz was associated with oxidative stress in the hippocampus and striatum, and impaired both spatial learning and habit learning. The study provides evidence for an association between impaired learning and oxidative stress in the hippocampus and striatum induced by ELF-MF exposure.
Lee HJ, Jin YB, Lee JS, Choi JI, Lee JW, Myung SH, Lee YS. · 2012
NIH3T3 cells were exposed to a 60 Hz ELF-MF at 1 mT for 4 hours, alone or with ionizing radiation, hydrogen peroxide, or c-Myc overexpression. A 4 hour exposure to ELF-MF alone produced no cell transformation. ELF-MF exposure did not influence the transformation activity of ionizing radiation, hydrogen peroxide, or activated c-Myc, indicating no additive or synergistic effects.
Sakhnini L, Al Ali H, Al Qassab N, Al Arab E, Kamal A. · 2012
Researchers investigated the possible effect of ELF-EMFs on motor performance in prenatal and neonatal exposed mice. Motor performance was evaluated using rotarod experiments after 5 days of subacute exposure. Prenatal exposed groups showed a pronounced deficit in learning abilities, but no pronounced effect was observed for the neonatal exposed group.
Hong MN et al. · 2012
Researchers exposed MCF10A human breast epithelial cells to 1 mT of 60 Hz ELF-MF for 4 hours and measured intracellular ROS level, SOD activity, and GSH/GSSG ratio. The ELF-MF exposed cells showed no statistically significant changes in these parameters compared to incubator controls or sham-exposed cells. In contrast, ionizing radiation exposed cells showed marked changes in ROS level, SOD activity, and GSH/GSSG ratio, and were positive for morphological changes and SA-β-Gal activity.
Cui Y, Ge Z, Rizak JD, Zhai C, Zhou Z, Gong S, Che Y. · 2012
Researchers exposed mice to power line frequency magnetic fields for 4 hours daily over 12 weeks. The exposed mice showed impaired learning and memory abilities, plus brain damage from oxidative stress. This suggests household electrical fields may affect cognitive function.
