Barteri M et al. · 2015
The study examined the effects of 900 MHz electromagnetic field exposure on the catalytic activity of lactoperoxidase and horseradish peroxidase. Experimental evidence indicates that irradiation causes conformational changes of the active sites and influences the formation and stability of intermediate free radicals, as shown by enzyme kinetics, circular dichroism spectroscopy, and cyclic voltammetry.
Barteri M et al. · 2015
Researchers exposed two important enzymes (lactoperoxidase and horseradish peroxidase) to 900 MHz radiofrequency radiation, the same frequency used by many cell phones. The radiation altered the enzymes' structure and function, changing how they catalyzed chemical reactions and affecting the stability of free radicals they produce. This suggests that RF radiation can directly interfere with fundamental biological processes at the molecular level.
Todorović D, Prolić Z, Petković B, Kalauzi A. · 2015
Researchers analyzed the bioelectrical activity of antennal lobe neurons in adult Morimus funereus beetles exposed to extremely low frequency magnetic fields of different waveforms and exposure durations. Sine wave magnetic fields did not change the reversibility factor of antennal lobe neuronal activity regardless of exposure duration, while square wave exposure for 10 and 15 minutes significantly changed the reversibility factors of the nearest neurons.
Li C, Wu T. · 2015
Researchers numerically compared dosimetric results among adult, child, and infant models to evaluate infant exposure to magnetic fields from an electronic article surveillance system operating at 125 kHz and 13.56 MHz. Postures did not significantly influence dosimetric results when the cross-sectional area under exposure was similar. Infants showed higher SAR values for brain and central nervous system tissues compared with adults, 1.5x at 125 kHz and 112x at 13.56 MHz, though safety limits were unlikely to be exceeded. The infant's specific anatomy, such as a non-proportionally large head and high fat content, did not lead to higher SAR values.
Percherancier Y et al. · 2015
Researchers used fluorescence recovery after photobleaching microscopy to measure dye diffusion between NIH3T3 fibroblasts through gap junctions. They exposed cells to 50 Hz magnetic fields at 0.4 or 1 mT for 24 hours and assessed direct effects on gap junctional intercellular communication. They also tested for synergism with the inhibitor phorbol ester by incubating cells with TPA for 1 hour after 24 hours of magnetic field exposure. Under these conditions, they observed neither direct inhibition of gap junctional intercellular communication nor synergism with TPA-induced inhibition.
Megha K et al. · 2015
Researchers exposed Fischer rats to 900 MHz and 1800 MHz microwave radiation for 30 days at 2 hours per day, 5 days per week, with specific absorption rates of 5.953 × 10−4 and 5.835 × 10−4 W/kg respectively. Levels of dopamine, norepinephrine, epinephrine, and serotonin were measured in the hippocampus using LC–MS/MS. Exposed animals showed significant reductions in DA, NE, E and 5-HT, and significant downregulation in mRNA expression of TH, TPH1, and TPH2 compared with sham-exposed animals (p < 0.05).
Zuo H et al. · 2015
Researchers exposed differentiated PC12 cells to continuous microwave radiation at 2.856 GHz for 5 minutes with an average power density of 30 mW/cm2. After exposure, RKIP was downregulated while the MEK/ERK/CREB signaling pathway was activated excessively. The ratio of Bcl-2/Bax decreased, caspase-3 activity increased, and apoptotic DNA fragmentation increased. The researchers suggested that RKIP may act as a key regulator of neuronal damage caused by microwave radiation.
Danker-Hopfe H et al. · 2015
Researchers conducted a double-blind, randomized, sham-controlled cross-over study to examine whether electromagnetic fields from GSM 900 and WCDMA/UMTS devices affected sleep. Thirty healthy young men between 18 and 30 years old were each exposed three times per exposure condition while their sleep was recorded. The only consistent finding across individuals was an increase of stage R sleep under GSM 900 MHz exposure in 9 of 30 subjects and under WCDMA/UMTS exposure in 10 of 30 subjects.
Tang J et al. · 2015
Researchers exposed 108 male Sprague-Dawley rats to a 900 MHz, 1 mW/cm2 electromagnetic field for 14 or 28 days at 3 hours per day, with some rats left unexposed as a sham group. Rats exposed for 28 days crossed platforms less often and spent less time in the target quadrant than rats exposed for 14 days and unexposed rats. Twenty-eight days of exposure was also associated with cellular edema, neuronal cell organelle degeneration, and albumin and HO-1 extravasation in the hippocampus and cortex. The researchers found that 28 days of EMF exposure induced the expression of mkp-1, resulting in ERK dephosphorylation.
Chung YH et al. · 2015
Researchers exposed rats to an extremely low frequency magnetic field at 60 Hz and 2.0 mT for 2 or 5 days, then measured neurotransmitter levels in the cortex, striatum, thalamus, cerebellum and hippocampus. The exposure produced significant differences in norepinephrine and vanillyl mandelic acid in the striatum, thalamus, cerebellum and hippocampus. Significant increases in serotonin and 5-hydroxyindoleacetic acid were observed in the striatum, thalamus or hippocampus, and dopamine concentration was significantly increased in the thalamus. The researchers concluded that exposure to ELF-MF may evoke changes in these brain chemicals, though the mechanisms remain to be characterized.
Patruno A, Tabrez S, Pesce M, Shakil S, Kamal MA, Reale M · 2015
Researchers evaluated the effect of extremely low frequency electromagnetic field exposure on catalase, cytochrome P450, and inducible nitric oxide synthase activity and protein expression in the myelogenous leukemia cell line K562. A significant modulation of iNOS, CAT, and cytochrome P450 protein expression was recorded in both stimulated and non-stimulated cell lines following ELF-EMF exposure. Changes in the kinetic parameters of these enzymes in response to ELF-EMF indicated an interaction between ELF-EMF and the enzymological system.
Cao H, Qin F, Liu X, Wang J, Cao Y, Tong J, Zhao H · 2015
Researchers exposed male Sprague Dawley rats to 1.8 GHz radiofrequency fields at different times of day to study whether circadian rhythms of plasma antioxidants were affected. Six groups of animals were exposed at a power density of 201.7 μW/cm2 and a specific absorption rate of 0.05653 W/kg for 2 hours per day for 32 consecutive days, each at a different time of day, while a seventh group served as sham-exposed controls. The circadian rhythms of melatonin, GSH-Px, and SOD were shifted in exposed rats, with levels significantly decreased when exposure was given at 23 and 3 h GMT. The researchers concluded that there may be adverse effects of radiofrequency exposure on antioxidant function, affecting both daily antioxidant levels and circadian rhythmicity.
Duan W et al. · 2015
Researchers exposed a mouse spermatocyte-derived GC-2 cell line intermittently to 50 Hz ELF-EMF at intensities of 1, 2, or 3 mT, or to 1800 MHz RF-EMF in GSM-Talk mode at SAR values of 1, 2, or 4 W/kg. After 24 hours of exposure, neither ELF-EMF nor RF-EMF affected cell viability. ELF-EMF exposure significantly increased DNA strand breaks at 3 mT, while RF-EMF exposure significantly induced oxidative DNA base damage at a SAR of 4 W/kg. The researchers suggest that both field types may produce genotoxicity at relatively high intensities but through different patterns of DNA damage.
Wang X et al. · 2015
Researchers exposed Neuro-2a cells to 900 MHz GSM signals at specific absorption rates of 0, 0.5, 1, or 2 W/kg for 24 hours, with or without silencing of the DNA repair enzyme OGG1. Without OGG1 silencing, 2 W/kg RF-EMFs induced oxidative DNA base damage, and with OGG1 silencing, damage was observed at exposures as low as 1 W/kg. Increases in oxidative DNA base damage were accompanied by similar increases in reactive oxygen species, but neither DNA strand breakage nor altered cell viability was observed.
Cheng Y et al. · 2015
Researchers examined the effects of extremely low-frequency electromagnetic fields on hippocampal neural progenitor cells cultured from embryonic and adult ischemic brains. Exposure to ELF-EMF at 50 Hz and 0.4 mT significantly enhanced proliferation in both embryonic and ischemic neural progenitor cells. Neuronal differentiation was also enhanced after 7 days of cumulative ELF-EMF exposure, while glial differentiation was not markedly influenced. The data suggest that ELF-EMF promotes neurogenesis of ischemic neural progenitor cells, possibly through the Akt pathway.
Chung YH et al. · 2015
Researchers exposed laboratory rats to 60 Hz magnetic fields (the same frequency as household electrical systems) for 2 to 5 days and found significant changes in brain chemistry. The magnetic field exposure altered levels of key neurotransmitters including serotonin, dopamine, and norepinephrine across multiple brain regions. These chemical messengers control mood, movement, attention, and other critical brain functions.
de Groot MW, van Kleef RG, de Groot A, Westerink RH · 2015
Researchers exposed primary rat cortical neurons for 7 days to 50 Hz ELF-EMF at field strengths ranging from 0 to 1000 μT, then measured cell viability, calcium levels, neurite outgrowth, and spontaneous neuronal activity. Cell viability was not affected by exposure across the full range tested, and neuronal activity appeared largely unaltered. The researchers concluded that chronic ELF-EMF exposure has only limited neurotoxic potential in vitro.
Jankowska M et al. · 2015
Researchers assumed that exposure to ELF-EMF would change the effects of a scorpion alpha toxin called LqhαIT on cockroach nerve function and on whole insects. Experiments were performed in vivo on cockroaches (Periplaneta americana) and in vitro on isolated cockroach abdominal nerve cord with cerci. The exposure to ELF-EMF modified the effects of LqhαIT on activity of the cercal nerve and of the connective nerve, with a decrease in the toxin effect on the cercal nerve but an increase on the connective nerve. Biotests showed that toxicity of LqhαIT on cockroaches was reduced by the exposure to ELF-EMF at 0.7 and 7 mT.
Zhao QR, Lu JM, Yao JJ, Zhang ZY, Ling C, Mei YA. · 2015
Researchers used a mouse model to examine the effects of extremely low-frequency (50 Hz) electromagnetic field exposure on a recognition memory task and morphological changes of hippocampal neurons. Exposure at 1 mT for 12 hours per day produced a time-dependent deficit in novel object associative recognition memory and decreased hippocampal dendritic spine density. The memory deficit was transient, observed only after exposing mice to the fields for 7 to 10 days, and occurred without changes in spontaneous locomotor activity. Over-expression of neuritin in the hippocampus using an adeno-associated virus vector significantly increased neuritin levels and dendritic spine density, and neuritin prevented the exposure-induced effect by increasing hippocampal spine density.
Cao H, Qin F, Liu X, Wang J, Cao Y, Tong J, Zhao H. · 2015
Researchers exposed rats to cell phone-level radiation for 2 hours daily over 32 days. The radiation disrupted natural 24-hour cycles of antioxidant production, with nighttime exposures causing the biggest drops in protective compounds like melatonin. This suggests RF radiation may interfere with the body's internal clock.
Masuda H et al. · 2015
The cortex tissue targeted was locally exposed to 1439 MHz RF using a figure-8 loop antenna at an averaged specific absorption rate of 2.0 W/kg in the target area for 50 min. Three microcirculatory parameters related to cerebral inflammation were measured by the cranial window method in real-time under RF exposure. No extravasation of intravenously injected fluorescent dye was observed during RF exposure. There was no significant difference either in pial venule blood flow velocity or diameter between exposed and sham-exposed rats. Histological evaluation for the brain immediately after RF exposure did not reveal any serum albumin leakage sites or degenerate neurons.
Şahin A et al. · 2015
The study consisted of control, sham-exposed, and EMF-exposed groups with six rats in each. The EMF-exposed group received 900 MHz EMF for 1 hour per day for 30 days while the sham group was placed in the same jar without exposure. Stereological analysis revealed a significantly lower total number of pyramidal neurons in the cornu ammonis of EMF-exposed rats compared to both control and sham groups, and histopathology showed abnormal cells with dark cytoplasm and shrunken morphology.
Bin-Meferij MM, El-Kott AF · 2015
İkinci A et al. · 2015
Researchers exposed young male rats to 900 MHz radiofrequency radiation (similar to cell phone frequencies) for one hour daily during adolescence and examined their spinal cords. They found significant damage including breakdown of the protective myelin sheaths around nerve fibers, increased oxidative stress markers, and structural abnormalities in nerve cells. This suggests that radiofrequency exposure during critical developmental periods may harm the developing nervous system.
Kazemi E et al. · 2015
The study investigated the effect of 900 MHz GSM on oxidative stress and intracellular ROS levels in human mononuclear cells, monocytes, and lymphocytes. Blood samples from 13 healthy volunteers were exposed to RF radiation from a mobile phone simulator for 2 hours, while control samples were not exposed. Mononuclear cells were isolated by density gradient centrifugation and ROS was measured using a CM-H2DCFDA fluorescence probe and flow cytometry. Results showed a significant increase in ROS production in monocyte-rich cell populations after exposure, but no significant change in lymphocyte-rich populations.
