Lantow M, Schuderer J, Hartwig C, Simko M. · 2006
Heat and PMA treatment significantly increased superoxide radical anions and ROS production in Mono Mac 6 cells. No significant differences in free radical production were detected after RF EMF exposure compared to controls. The GSM-DTX signal at 2 W/kg showed a significant difference versus sham due to a decreasing sham value, but this difference disappeared when compared to incubator controls. No significant effects on HSP70 expression were detected after RF EMF exposures.
Linet MS et al. · 2006
Researchers used a questionnaire to assess cellular telephone use in 551 non-Hodgkin lymphoma cases and 462 population controls. Compared to never users, the odds ratios for non-Hodgkin lymphoma were close to 1.0 for all categories of lifetime use, indicating no increased risk. The study also found little evidence linking cellular telephone use to specific non-Hodgkin lymphoma subtypes such as diffuse large B-cell lymphoma or follicular lymphoma. The authors noted that less than 5% of the population reported using cellular telephones for six or more years or for 200 or more cumulative hours.
Anghileri LJ, Mayayo E, Domingo JL, Thouvenot P. · 2006
The study aimed to evaluate health risks caused by radio frequency accelerated carcinogenesis and the importance of processes driven by the calcium ion signal. Exposed animals showed organ hypertrophy, lymphoid blood and ascites, solid tumours, and mortality that were very different from control animals. The results emphasized the role of calcium ion signal influx in the activation of oncogenes and the failure of thymus-determined immune defences.
Trosic I, Busljeta I. · 2006
Researchers exposed rats to 2.45 GHz microwave radiation at a whole-body SAR of 1.25+/-0.36 W/kg for 2 hours per day, 7 days a week. Bone marrow polychromatic erythrocytes increased on days 8 and 15, and peripheral blood polychromatic erythrocytes increased on days 2 and 8. Bone marrow micronucleated PCE frequency increased on day 15, and peripheral blood micronucleated PCEs increased on day 8, but all values declined nearly to control levels by the end of the experiment.
Stankiewicz W et al. · 2006
Researchers investigated whether human blood immune cells activated in vitro would be sensitive to electromagnetic field exposure. The results suggest that immune activity of responding lymphocytes and monocytes can be additionally intensified by 900 MHz microwaves.
Capri M et al. · 2006
Researchers exposed human peripheral blood mononuclear cells from young and elderly donors to radiofrequency radiation at 1,800 MHz and a Specific Absorption Rate of 2 W/kg, with or without mitogenic stimulation. However, RF exposure produced a slight but significant downregulation of CD95 expression in stimulated CD4+ T lymphocytes from elderly donors only.
Reale M et al. · 2006
Researchers investigated whether overnight exposure to a 1 mT, 50-Hz sinusoidal electromagnetic field affects the expression of iNOS and MCP-1 in human monocytes. EMF exposure affected iNOS and MCP-1 expression in cultured human mononuclear cells at both the mRNA level and the protein level. iNOS was down-regulated while MCP-1 was up-regulated.
Frahm J, Lantow M, Lupke M, Weiss DG, Simkó M · 2006
Researchers investigated whether extremely low frequency electromagnetic fields affect cellular functions and immunologic parameters of mouse macrophages. Magnetic field exposure led to a 1.6-fold increased uptake of latex beads in exposed cells compared to controls after 45 minutes. IL-1β formation increased significantly after 4 hours and reached a maximum 12.3-fold increase after 24 hours compared to controls. All tested flux densities significantly stimulated free radical formation, and no significant differences in micronucleus formation were found in exposed cells.
Unknown authors · 2005
The study found that human lymphocyte-synthesized melatonin plays a crucial role in modulating the IL-2/IL-2 receptor system. When melatonin biosynthesis was blocked with parachlorophenylalanine, both IL-2 and IL-2 receptor levels fell, and adding exogenous melatonin restored them. The effects were mediated by melatonin receptors, as antagonists luzindole and CGP 55644 significantly decreased IL-2 and IL-2 receptor expressions. Blocking membrane receptors with luzindole increased prostaglandin E2-induced inhibition of IL-2 production.
Unknown authors · 2005
Researchers exposed two-month-old Wistar male rats to 50 Hz electromagnetic fields at 100-300 microT and 54-160 V/m for 4 h a day, seven days a week for one month. After sacrifice, skin and thyroid samples were processed for toluidine blue staining or indirect immunohistochemistry, and stereological analysis was performed using an M42 grid and a special microscopic frame. The numerical and volume density of intact type A mast cells in the thyroid was significantly higher in the exposed group compared to controls. Mast cells and immunoreactive nerve fibers were observed in the skin, and histamine-immunoreactive mast cells were observed in the thyroid of exposed animals. However, the differences in stereological data were not statistically significant by the Mann-Whitney test.
Lim HB, Cook GG, Barker AT, Coulton LA. · 2005
Researchers exposed human peripheral blood to 900 MHz fields at specific absorption rates of 0.4, 2.0, and 3.6 W/kg for durations of 20 minutes, 1 hour, and 4 hours. Heat stress at 42 degrees Celsius increased the number of cells expressing HSP70 and HSP27 in a time-dependent manner. No statistically significant difference in stress protein expression was detected after RF-field exposure.
Zeni O et al. · 2005
Researchers exposed human peripheral blood leukocytes from healthy volunteers to a 900 MHz GSM signal for 2 hours at SARs of 0.3 and 1 W/kg. DNA damage was assessed with the comet assay, chromosome aberrations and sister chromatid exchanges were measured in stimulated lymphocytes, and cell proliferation was evaluated via the mitotic index. No statistically significant differences were observed between exposed and sham-exposed samples for any of these endpoints, while positive controls showed the expected increases in DNA damage.
Hardell L, Eriksson M, Carlberg M, Sundstrom C, Mild KH. · 2005
B-cell non-Hodgkin's lymphoma was not associated with the use of cellular or cordless telephones. For T-cell non-Hodgkin's lymphoma with a latency period of more than five years, cordless phone use showed an odds ratio of 2.47 with a 95% confidence interval of 1.09 to 5.60. The authors noted that the results indicate an association between T-cell NHL and phone use but are based on low numbers and must be interpreted with caution.
Gandhi G, Anita · 2005
Researchers investigated DNA and chromosomal damage in peripheral blood lymphocytes of 24 mobile phone users exposed to microwave frequencies of 800-2000 MHz. DNA damage measured by comet assay showed a mean comet tail length of 26.76 ± 0.054 mm with 39.75% of cells damaged, which was highly significant compared to controls. The in vivo capillary blood micronucleus test also revealed a highly significant frequency of micronucleated cells at 0.25.
Yariktas M et al. · 2005
Trosic I, Busljeta I. · 2005
Researchers exposed Wistar rats to 2.45 GHz continuous RF/MW radiation for 2 hours daily, 7 days a week, at 5–10 mW/cm², with subgroups receiving 4, 16, 30, and 60 total hours of exposure. The frequency of micronucleated bone marrow erythrocytes was significantly increased after 15 irradiation treatments. No statistically significant differences in micronucleus frequency were observed after 2, 8, or 30 irradiation treatments.
Markovà E, Hillert L, Malmgren L, Persson BR, Belyaev IY. · 2005
Researchers investigated the effects of GSM microwaves at different carrier frequencies on human lymphocytes from healthy persons and from persons reporting hypersensitivity to electromagnetic fields. They measured changes in chromatin conformation and analyzed proteins 53BP1 and gamma-H2AX, which have been shown to colocalize in distinct foci with DNA double-strand breaks. GSM microwaves affected chromatin conformation and 53BP1/gamma-H2AX foci similar to heat shock, and on average the same response was observed in lymphocytes from hypersensitive and healthy subjects.
Belyaev IY et al. · 2005
Researchers exposed human lymphocytes from healthy subjects and from persons reporting hypersensitivity to electromagnetic field (EMF) to 915 MHz microwaves and 50 Hz magnetic field, then measured chromatin conformation and markers of DNA damage. Both exposures caused significant chromatin condensation, similar to heat shock at 41 °C, but no significant differences were found between hypersensitive and healthy subjects. Neither 915 MHz nor 50 Hz exposure induced 53BP1 foci, and no apoptosis was induced.
Unknown authors · 2004
The authors conducted an extensive literature review and propose that ELF EMF exposure increases free radical levels. They suggest EMF might induce an activated state such as phagocytosis, enhancing free radical release and leading to genotoxic events. The proposed mechanisms include direct activation of macrophages leading to phagocytosis and free radical production, and direct stimulation of free radical production by EMF-induced macrophage activation. They also propose that EMF increases the lifetime of free radicals causing persistently elevated concentrations, and that long-term exposure leads to chronically increased free radicals that inhibit melatonin effects. Taken together, these reactions could lead to higher DNA damage incidence and increased tumor risk.
Capri M et al. · 2004
Researchers studied whether prolonged in vitro exposure to 1800 MHz radiofrequency could affect human peripheral blood mononuclear cells from young and elderly donors by altering apoptosis, mitochondrial membrane potential, and heat shock protein 70 levels. Three GSM signal modulations (Basic, DTX, and Talk) were applied at specific absorption rates of 1.4 and 2.0 W kg−1. Endpoints were analyzed both with and without the apoptosis-inducing agent 2-deoxy-D-ribose. No significant difference was found between RF-exposed and sham-exposed cells for any endpoint. The 1800 MHz RF did not induce apoptosis on its own, did not alter apoptosis triggered by an agent, and did not change mitochondrial function or HSP 70 expression.
Hook GJ et al. · 2004
Researchers exposed Molt-4 T lymphoblastoid cells to CDMA, FDMA, iDEN, and TDMA modulated RF radiation for up to 24 hours. DNA damage was measured with the single-cell gel electrophoresis assay and apoptosis with the annexin V affinity assay. No statistically significant difference in DNA damage or apoptosis was observed between sham and exposed cells for any frequency, modulation, or exposure time.
Hook et al. · 2004
Researchers exposed J774.16 mouse macrophage cells to FMCW radiation at 835.62 MHz and CDMA radiation at 847.74 MHz for 20–22 hours at a specific absorption rate of 0.8 W/kg. Cells were stimulated with γ-interferon and bacterial lipopolysaccharide before exposure. The results indicated that FMCW- and CDMA-modulated RF radiation did not alter parameters indicative of oxidative stress in J774.16 cells.
Kimata H. · 2004
Writing mail on a mobile phone increased plasma nerve growth factor, neurotrophin-3, and allergic skin wheal responses in atopic dermatitis patients. Viewing a comic video reduced these neurotrophin levels and allergic skin responses, while a nonhumorous video did not. Previewing the comic video also counteracted the mobile phone-mediated enhancement of neurotrophins and allergic skin wheal responses, whereas the weather video did not.
Demsia G, Vlastos D, Matthopoulos DP. · 2004
The study aimed to investigate whether 910-MHz electromagnetic fields could be a noxious agent capable of inducing genotoxicity, and rats were exposed daily for 2 h over 30 consecutive days. Researchers examined bone marrow smears from EMF-exposed and sham-exposed animals and observed an almost threefold increase in micronuclei in polychromatic erythrocytes. An induction of micronuclei was also observed in polymorphonuclear cells.
Ji S, Oh E, Sul D, Choi JW, Park H, Lee E. · 2004
Researchers used the Comet assay to measure Olive Tail Moment and Tail DNA% in T-cells, B-cells, and granulocytes before and after exposure. Olive Tail Moment and Tail DNA% in B-cells and granulocytes increased significantly after exposure.
