Hagiwara S, Iwasaka H, Takeshima N, Noguchi T. · 2009
Sciatic nerves were treated with PRF at 37 degrees and 42 degrees, which inhibited hyperalgesia in the inflammatory groups when compared to RF and sham treatment. This effect was attenuated after intrathecal administration of the alpha2-adrenoceptor antagonist yohimbine, the selective 5-HT3 serotonin receptor antagonist MDL72222, and the non-selective serotonin receptor antagonist methysergide. All three drugs were found to significantly inhibit the analgesic effect of PRF. The results suggest that the analgesic action of PRF involves the enhancement of noradrenergic and serotonergic descending pain inhibitory pathways.
Gul A, Celebi H, Uğraş S. · 2009
Researchers exposed pregnant rats to mobile phones during pregnancy and examined ovarian follicles in their female offspring at 21 days old. The study group had fewer ovarian follicles than the control group. The authors suggest that intrauterine microwave exposure has toxic effects on ovaries.
Anghileri LJ, Mayayo E, Domingo JL. · 2009
The study found a synergism between aluminum and radiofrequency that increased early mortality in lymphoma-bearing mice. This synergism was stronger with aluminum lactate than with aluminum citrate due to higher ionization at physiological pH. The dissociation of aluminum complexes appears to induce a remarkable acceleration of mortality and lymphoid-related hypertrophy of the spleen and liver at an early age. Calcium chloride overload had no effect when cellular calcium homeostasis was functional. The researchers measured organ hypertrophy using the organ-to-body-weight ratio and histopathological examination.
Adang D, Remacle C, Vorst AV. · 2009
Researchers exposed four-month-old Wistar albino rats to two different microwave frequencies and exposure modes for 2 hours a day, seven days a week, over 21 months. They measured leucocytes, erythrocytes, monocytes, neutrophils, lymphocytes, hemoglobin, mean corpuscular hemoglobin concentration, and mortality rate. After three and eight months, monocytes differed by about 20% in the 970-MHz continuous wave group; after 14 and 18 months, white blood cells increased about 15% and neutrophils about 25%; lymphocytes fell about 15% at 18 months. Mortality in exposed groups increased after 21 months at age 25 months and reached almost twice the sham-exposed value by age 28 months.
Mailankot M et al. · 2009
Researchers exposed male albino Wistar rats to RF-EMR from an active GSM mobile phone for one hour daily over 28 days. Exposed rats had significantly reduced sperm motility but no change in total sperm count. RF-EMR exposure also increased lipid peroxidation and decreased glutathione levels in the testis and epididymis. Facial temperature did not change significantly during exposure.
Balci M, Namuslu M, Devrim E, Durak I · 2009
Four groups of ten Wistar rats each were studied for three weeks. Rats in the computer and computer plus vitamin C groups were exposed to computer monitors, and vitamin C was given orally at 250 mg/kg/day. In corneal tissue, monitor exposure increased malondialdehyde levels and catalase activity, while vitamin C further raised malondialdehyde, superoxide dismutase, and glutathione peroxidase activities and lowered catalase activity. In lens tissue, monitor exposure raised malondialdehyde and glutathione peroxidase activities and increased superoxide dismutase, while vitamin C increased superoxide dismutase and decreased catalase activity.
Narayanan SN, Kumar RS, Potu BK, Nayak S, Mailankot M · 2009
Researchers exposed male Wistar rats to 50 missed calls per day for four weeks from a GSM mobile phone in vibratory mode. After exposure, the rats were tested for spatial memory using the Morris water maze test. Both exposed and control rats decreased their escape time with training. Phone-exposed rats had about three times higher mean latency to reach the target quadrant and spent about two times less time in it than controls.
Kumar RS, Sareesh NN, Nayak S, Mailankot M. · 2009
The study aimed to determine whether mobile phone exposure causes changes in rat behavior on the elevated plus maze. Rats were divided into control and exposure groups of six each. Exposed rats were tested at one hour and 24 hours after exposure, while controls were tested with the 24-hour group. The researchers stated that preliminary results indicate mobile phone exposure induced a deficit in open arm exploration on the elevated plus maze.
Xu Q, Tong J, Jin ZD, Lu MX, DU HB, Cao Y. · 2009
Researchers randomly assigned 96 healthy Kunming mice to four groups: normal control, microwave (120 microW/cm(2), 900 MHz), gamma-ray irradiation (5 Gy), and combined microwave plus gamma-ray (120 microW/cm(2) + 5 Gy). The microwave and combined groups were exposed to 120 microW/cm(2) microwave for 1 h/d for 14 days before the gamma-ray and combined groups received 5 Gy (60)Co gamma-ray irradiation on the 15th day. Bone marrow pathology in the combined exposure group was milder and recovered faster than in the gamma-ray only group. Splenic cell apoptosis was significantly lower in the combined group on days 6 and 9 after irradiation (23.02% +/- 15.18% and 25.37% +/- 11.62%). Serum superoxide dismutase increased and lipid peroxide decreased significantly in the combined group (P < 0.05).
Moisescu MG, Leveque P, Verjus MA, Kovacs E, Mir LM. · 2009
The exposure increased Lucifer Yellow uptake, and this increase was not due to heating. Chlorpromazine and ethanol blocked the increase while Filipin did not, indicating involvement of clathrin-coated vesicles.
Li X et al. · 2009
Researchers exposed 70 male rats to microwave radiation at average power densities of 0, 10, 30, and 100 mW/cm(2). Rats were sacrificed at 6 hours, 1 day, 3 days, and 7 days after exposure. Protein levels of AQP4 increased initially and then recovered at 10 and 30 mW/cm(2), but increased progressively at 100 mW/cm(2) within 14 days. Gene expression of AQP4 increased at 6 hours after 10 mW/cm(2) exposure and returned to baseline, while at 30 and 100 mW/cm(2) it peaked at 7 days and did not return.
Gao XF,Pei LP, Chen CH, Yang XS, Zhang GB, Deng ZH, Yu ZP. · 2009
Researchers exposed rats to whole-body microwave irradiation at 90 and 5 W/cm² for 20 minutes and measured changes in HSP70 in the hippocampus. Both mRNA and protein expression of HSP70 in the rat hippocampus increased after the exposures. Anal temperature and SAR values also increased significantly, and these changes were positively correlated with power and irradiation time.
Cao Y, Xu Q, Lu MX, Jin ZD, DU HB, Li JX, Nie JH, Tong J. · 2009
Researchers treated male Kunning mice with low-dose microwave radiation before exposing them to gamma-ray irradiation, then measured survival, blood parameters, and markers of bone marrow activity. Mice pre-exposed to 120 microW/cm² 900 MHz microwave had a higher 30-day survival rate and more white blood cells compared with gamma-ray-treated mice that did not receive microwave pre-exposure. The microwave pre-exposure group also showed increases in thymus and spleen organ indexes, bone marrow cell proliferation activity, CFU-GM hematopoietic foci numbers, and serum concentrations of GM-CSF and IL-3. The researchers suggested that low-dose microwave radiation may exert potential antagonistic effects on hematopoietic injuries induced by ionizing radiation.
Amara S et al. · 2009
Researchers exposed male rats to a static magnetic field at 128 mT for one hour per day over 30 consecutive days and then measured antioxidant enzyme activity, malondialdehyde concentration, and DNA oxidation in the brain. In the frontal cortex, exposure decreased glutathione peroxidase activity by 39%, CuZn superoxide dismutase activity by 35%, and catalase activity by 59%. In the hippocampus, exposure decreased CuZn-SOD activity by 51% and Mn-SOD activity by 13%, and increased malondialdehyde concentration by 32%. DNA oxidation was not detected, and the researchers suggested that an increase in metallothioneins in the frontal cortex may have protected DNA from oxidative damage.
Varró P, Szemerszky R, Bárdos G, Világi I. · 2009
Researchers exposed whole rats or excised rat brain slices to a 50 Hz electromagnetic field at 250–500 µT to examine effects on synaptic efficacy in the central nervous system. Slices treated directly ex vivo showed a decrease in basic synaptic activity, observed as a diminution in amplitude of evoked potentials. Following whole-body exposure, enhanced short- and long-term synaptic facilitation in hippocampal slices and increased seizure susceptibility in neocortical slices were observed. The researchers noted that the overall changes may strongly depend on the synaptic structure and neuronal network of the affected region, together with the specific spatial parameters and constancy of the EMF.
Strasák L, Bártová E, Krejci J, Fojt L, Vetterl V. · 2009
Researchers exposed female and male laboratory mice for 4 days to a 50 Hz magnetic field at 2 mT inside a cylindrical coil. After exposure, levels of the proteins c-Jun and c-Fos in different parts of the brain were measured using Western-blot techniques. The expression of c-Jun decreased after magnetic field exposure, while c-Fos expression was not affected, and neither result depended on the sex of the mice.
Janać B, Tovilović G, Tomić M, Prolić Z, Radenović L. · 2009
Researchers exposed adult Wistar rats continuously to an extremely low frequency magnetic field (50 Hz, 0.5 mT) for 1, 3, and 7 days. The study examined serotonin 5-HT(2A) receptors in the prefrontal cortex and dopamine D(1) and D(2) receptors in the striatum, considering their involvement in motor behavior regulation. Serotonin 5-HT(2A) receptor affinity decreased and density increased in the prefrontal cortex, with the affinity effect being most prominent after 7 days of exposure. Dopamine D(1) and D(2) receptors in the striatum showed no significant effect on either affinity or density.
Di Loreto S et al. · 2009
Researchers investigated the effects of exposures to two different 50 Hz sinusoidal ELF magnetic field intensities, 0.1 and 1 mT, on maturing rat cortical neurons. Exposure positively affected cell viability and reduced apoptotic death, with no significant effects on the main antioxidative defences. Linear regression analysis suggested a positive correlation between reduced glutathione and ROS levels in 1 mT magnetic-field-exposed cells. The researchers also observed brain-derived neurotrophic factor over-expression and interleukin 1β-dependent regulation of pro-survival signaling pathways following exposure.
Balassa T, Szemerszky R, Bárdos G. · 2009
Researchers exposed rats to a 50 Hz electromagnetic field and then tested situational and social anxiety as well as locomotor activity. The tests used included the elevated plus-maze, novel object exploration, social interaction, and territoriality assessments. The researchers found that the field parameters may cause discomfort and may influence behavior, but there was no verified effect on social and territorial behavior.
Naziroğlu M, Gümral N. · 2009
Researchers divided thirty rats into five groups to study the effects of 2.45 GHz electromagnetic radiation on the brain's antioxidant system and EEG records. Exposed rats received 2.45 GHz electromagnetic radiation for 60 minutes per day for 28 days, after which EEG records and brain cortex samples were collected. Brain cortex concentrations of vitamin A, vitamin C, and vitamin E were lower in the exposed group than in controls, though these concentrations were increased by selenium and L-carnitine supplementation. L-carnitine appeared to have a more protective effect on vitamin concentrations than selenium.
López-Martín E et al. · 2009
Researchers studied whether pulse-modulated GSM radiation had different effects on brain activity than an unmodulated signal of the same wavelength, and examined c-Fos expression in seizure-related brain circuits. Rats made seizure-prone with picrotoxin and then exposed to GSM radiation showed differences in clinical and EEG signs and in c-Fos expression compared with picrotoxin-treated rats exposed to unmodulated radiation. The most marked effects of GSM radiation on c-Fos expression in picrotoxin-treated rats were observed in limbic structures, olfactory cortex areas, subcortical areas, the dentate gyrus, and the central lateral nucleus of the thalamic intralaminar nucleus group. Neither radiation treatment caused tissue heating, so the researchers ruled out thermal effects.
Del Vecchio G et al. · 2009
Dasdag S, Akdag MZ, Ulukaya E, Uzunlar AK, Ocak AR · 2009
The study used 31 Wistar Albino adult male rats, with the exposed group having their heads placed in a carousel and exposed to 900 MHz microwave radiation for 2 hours per day, 7 days a week, for 10 months. The apoptosis score in exposed rats was significantly lower than in both the sham group and the cage control group. Total antioxidant capacity and catalase were higher in the exposed group than in the sham group, while the oxidative stress index showed no significant difference between those groups. The protein p53 was not significantly changed by the exposure.
Daniels WM, Pitout IL, Afullo TJ, Mabandla MV · 2009
Researchers studied the effect of electromagnetic radiation in the mobile phone range on the behaviour of the rat. Behavioural, histological and biochemical tests were performed on exposed and unexposed male and female rats to examine learning and memory, emotional states and corticosterone levels. No significant differences were found in spatial memory or brain morphology between groups, but some exposed animals showed decreased locomotor activity, increased grooming and a tendency of increased basal corticosterone levels. The researchers suggested that EMR exposure may lead to abnormal brain functioning.
Bas O, Odaci E, Kaplan S, Acer N, Ucok K, Colakoglu S · 2009
Researchers investigated the number of pyramidal cells in the hippocampus of 16-week-old female rats following postnatal exposure to 900 MHz EMF. Rats in the EMF group were exposed to 900 MHz EMF for 1 hour per day for 28 days, with a SAR ranging from 0.016 (whole body) to 2 W/kg (locally in the head). Postnatal EMF exposure caused a significant decrease of the pyramidal cell number in the CA, and cell loss was also visible at qualitative observation.
