Shallom JM et al. · 2002
Researchers exposed chick embryos to athermal 915 MHz microwave radiation to test for induction of the stress protein Hsp70. Hsp70 levels increased within 2 hours, reaching a maximum about 30% above controls by 3 hours. Embryos pre-exposed to microwave radiation before hypoxic stress had significantly higher survival after re-oxygenation than non-exposed controls (P < 0.05).
Leszczynski D, Joenväärä S, Reivinen J, Kuokka R · 2002
Researchers exposed human endothelial EA.hy926 cells to 900 MHz GSM mobile phone radiation for 1-hour and observed changes in phosphorylation of many proteins. The exposure transiently increased phosphorylation of hsp27 and altered expression levels of hsp27 and p38MAPK, and this phosphorylation increase was prevented by a p38MAPK inhibitor. The culture temperature remained at 37 +/- 0.3 degrees C throughout the exposure, confirming non-thermal effects. The authors hypothesized that mobile phone radiation-induced activation of hsp27 may facilitate brain cancer by inhibiting the cytochrome c/caspase-3 apoptotic pathway and increase blood-brain barrier permeability by stabilizing endothelial cell stress fibers.
Testylier G, Tonduli L, Malabiau R, Debouzy JC · 2002
Rats were exposed to 2.45 GHz continuous wave at 2 or 4 mW/cm(2) for 1 h during the day, or to 800 MHz modulated at 32 Hz at 200 mW/cm(2) for 1 or 14 h during the night. Exposure at 4 mW/cm(2) caused a significant 40% decrease in mean acetylcholine release from hippocampus, maximal at 5 h post exposure. Fourteen-hour nighttime exposure at 800 MHz induced a significant 43% decrease in acetylcholine release during 11 p.m.-4 a.m. compared to control rats.
Di Carlo A, White N, Guo F, Garrett P, Litovitz T. · 2002
Chick embryos were exposed to ELF-EMFs at 8 microT continuously for 4 days, or to ELF or RF EMFs repeated daily for 4 days in sessions of 20, 30, or 60 minutes once or twice daily. Several exposure protocols produced statistically significant decreases in protection against hypoxic stress, including continuous ELF and 30 or 60 minute ELF twice daily, or 30 or 60 minute once daily RF. Following 4 days of ELF-EMF exposure, HSP70 levels declined by 27% compared to controls.
Bisht KS, Moros EG, Straube WL, Baty JD, Roti Roti JL · 2002
Researchers exposed C3H 10T½ cells to 835.62 MHz FDMA or 847.74 MHz CDMA modulated radiofrequency radiation to determine whether it caused micronuclei to form. Cells were exposed at power levels of 3.2 or 4.8 W/kg for CDMA and 3.2 or 5.1 W/kg for FDMA, for durations of 3, 8, 16, or 24 hours. In three repeat experiments, no exposure condition produced a significant increase in micronuclei compared to sham-exposed cells.
Stagg RB et al. · 2001
Researchers exposed tube-restrained Fischer 344 rats to pulse-modulated 1.6 GHz Iridium cell phone fields at brain SARs of 0.16, 1.6 and 5 W/ kg for 2 hours. Core body temperature increased transiently by about 0.3 degrees C during the first 30 minutes of loose-tube restraint in conditioned animals. Immobilization alone caused a nearly 10-fold rise in ACTH and corticosterone in conditioned rats, and non-conditioned rats showed 3-10-fold higher hormone levels at 120 minutes. RF exposure did not change core body temperature, corticosterone, ACTH, or brain Odc, Fos, Jun mRNA compared with sham-exposed immobilized animals. The authors concluded that the pulse-modulated digital Iridium RF field at SARs up to 5 W/kg is incapable of altering these stress-related responses.
Cheever KL et al. · 2001
Researchers exposed pregnant rats to 10 MHz radiofrequency radiation and measured metabolism and distribution of 2-methoxyethanol and its metabolite methoxyacetic acid. Radiofrequency exposure reduced total body clearance of radiolabeled 2-methoxyethanol but did not change methoxyacetic acid levels in serum, embryos, or extraembryonic fluid. Protein adduct formation in embryos did not differ in a way that would explain the reported synergy between radiofrequency radiation and 2-methoxyethanol.
Anglesio L et al. · 2001
Radiofrequency field monitoring was carried out in Torino, Italy, to evaluate how electromagnetic field strength varies with height, location, and frequency. The study separated contributions from broadcasting antennas and mobile phone base stations. Theoretical evaluation added emissions from all base station installations in Torino and examined calculated field strength maps considering orography at different heights. Theoretical values were compared with measured values in the mobile telephony frequency range.
Mausset A, de Seze R, Montpeyroux F, Privat A · 2001
Researchers injected rats with gamma-vinyl-GABA to increase GABA levels and then used immunohistochemistry and image analysis to measure cerebellar GABA content. They exposed rats to 900 MHz pulsed radiofrequency at 4 W/kg SAR and continuous waves at 32 W/kg SAR. Pulsed exposure selectively reduced the stained processes area in the Purkinje cell layer, while continuous exposure decreased optical density in all three cell layers. The authors noted that it is unknown whether these effects are partly due to local tissue heating.
Cranfield CG, Wood AW, Anderson V, Menezes KG. · 2001
Researchers tested whether simulated GSM mobile phone signals at 915 MHz and 2 W kg(-1) influence calcium concentration or calcium signalling patterns in a human lymphocyte cell line. No significant changes were found in the regression slope of calcium fluorescence, mean calcium concentration, number of calcium spikes, or mean height of those spikes. A significant alteration in average frequency from Fourier spectra was found in one category only: pulsed wave exposure of activated cells.
Boscol P et al. · 2001
The study investigated the immune systems of 19 women with a mean age of 35 years who had been exposed to electromagnetic fields from radiotelevision broadcasting stations in their residential area for at least 2 years, with a mean exposure duration of 13 years. The exposed group had mean field levels of 4.3 plus or minus 1.4 V/m in the range 500 KHz to 3 GHz, while the control group had nearby residential exposure of less than 1.8 V/m. The exposed women showed statistically significant reductions in several immune cell types and lower in vitro production of IL-2 and interferon-gamma by peripheral blood mononuclear cells. The researchers concluded that high frequency electromagnetic fields reduce cytotoxic activity in the peripheral blood of women without a dose-response effect.
Schilling CJ · 2000
The study investigated effects of exposure to very high frequency radiofrequency radiation on six antenna engineers in two separate incidents. The first incident occurred in 1995 and the second in 1996. Six men were likely accidentally exposed to high levels of VHF radiofrequency radiation at 100 MHz while working on transmission masts, with four men in one incident and two in another. They experienced symptoms and signs including headache, paresthesiae, diarrhea, malaise, and lassitude. The condition of four men, two from each incident likely to have had the highest exposure, showed no significant improvement.
Kulkybaev GA, Pospelov NI · 2000
The study aimed to investigate changes in gastric electric activity and serum catecholamine levels under the influence of electromagnetic microwaves. Chronic experiments on 17 dogs revealed that ultrahigh-frequency electromagnetic waves applied to the epigastric area and head induced a double-phase response. During exposure, gastric electric activity was depressed and total catecholamines increased. After exposure, gastric activity was higher and epinephrine and norepinephrine levels were lower at 24 hours after each of 10 procedures and during 7 days after the 10 procedures.
de Pomerai D et al. · 2000
Researchers exposed nematode worms (C. elegans) overnight to 750-MHz microwaves at a SAR of 0.001 W/kg and measured levels of heat-shock proteins (HSPs). The worms showed an increase in HSPs following that exposure. The authors argued that the effect on HSPs was non-thermal and suggested that 'current exposure limits for microwave equipment may need to be reconsidered.'
Li et al. · 1999
Researchers exposed human fibroblast cells to 837 MHz continuous microwaves for 2 hours at SAR levels of 0.9 or 9.0 W/kg. TP53 protein levels were measured by Western blot at 2, 8, 24, and 48 hours after exposure. No significant changes in TP53 protein expression were found in exposed cells compared to sham or untreated cells at any time point. No morphological alterations were observed in microwave-treated cells compared to sham-treated cells.
Goswami PC et al. · 1999
Researchers exposed C3H 10T 1/2 mouse embryo fibroblasts to 835.62 MHz FMCW or 847.74 MHz CDMA microwaves at an average SAR of 0.6 W/kg and measured proto-oncogene expression. The exposures did not alter proto-oncogene expression kinetics after serum stimulation, nor did they change Jun and Myc mRNA levels or AP1, AP2, and NF-kappaB DNA-binding activity during the transition to plateau phase. However, 835.62 MHz FMCW exposure increased Fos mRNA about 2-fold in exponential and plateau-phase cells, and 847.74 MHz CDMA exposure increased it 1.4-fold.
Morrissey JJ et al. · 1999
Researchers investigated the effects of a one-hour exposure of mice to a 1.6-GHz radiofrequency signal, either continuous wave or pulse-modulated at 11 Hz (IRIDIUM), on c-fos gene expression in the brain. C-fos expression was not significantly elevated until exposure levels exceeded six times the peak dose and 30 times the whole-body average dose relative to human cellular telephone exposure limits. The pattern of c-fos elevation in limbic cortex and subcortex areas was consistent with a stress response due to thermal perception and restraint, not a direct interaction of IRIDIUM energy with brain tissue.
Fink JM, Wagner JP, Congleton JJ, Rock JC · 1999
This study evaluated police officers' exposures to microwaves emitted by traffic radar units, with measurements taken at approximated ocular and testicular levels of officers seated in patrol vehicles. Fifty-four radar units covering 17 different models, 4 frequency bands, and 3 antenna configurations were provided by four speed-enforcement agencies and one transportation research institute. The researchers concluded that police officer exposure to microwave radiation is apparently minimal.
Imaida et al. · 1998
Researchers exposed male F344 rats to a 1.439 GHz TDMA electromagnetic near-field for 90 minutes a day, 5 days a week, over 6 weeks. The rats were initiated with diethylnitrosamine and underwent partial hepatectomy at week 3. Peak liver SARs ranged from 1.91 to 0.937 W/kg and whole-body average SARs from 0.680 to 0.453 W/kg at 0.33 W antenna power. Exposure did not significantly alter the numbers or areas of GST-P-positive foci in the liver compared to sham-exposed rats. Serum levels of corticosterone, ACTH, and melatonin were increased in exposed rats.
de Seze R, Fabbro-Peray P, Miro L · 1998
Researchers measured adrenocorticotropin, thyrotropin, growth hormone, prolactin, luteinizing hormone, and follicle stimulating hormone in nine weekly blood samples taken from three weeks before exposure to two weeks after. Five of the six hormones showed no difference across samples, while thyrotropin decreased 21% on the seventh sample but returned to baseline afterward.
Kwee S, Raskmark P · 1998
Researchers exposed transformed human epithelial amnion cells to 960 MHz microwave fields at three power levels and three exposure times using a TEM cell. Cell growth was reduced in exposed cells compared to control and sham-exposed cells. The proliferation response varied with SAR level and exposure duration, showing a linear correlation between power level and growth change. At lower power levels, a longer exposure time was needed to reach the maximum effect, and repeated exposures did not alter the outcome.
Daniells et al. · 1998
Researchers used transgenic Caenorhabditis elegans strain PC72 carrying a heat-shock promoter driving a beta-galactosidase reporter gene. The nematodes were exposed in a transverse electromagnetic cell at 750 MHz and 27 dBm, with the cell held at 25°C in a shielded incubator. Significant stress responses were observed at 2 and 16 hours but not at 4 or 8 hours. Lower power levels of 21 dBm produced greater responses than 27 dBm at both 750 and 300 MHz, a pattern opposite to that expected from simple heating. In a 5 x 5 multiwell plate, wells in rows nearest the exposure showed the strongest response, while the most distant row did not rise above control levels, suggesting a shadow effect.
Loscher W, Kas G, · 1998
Researchers observed behavioral abnormalities, reduced milk yield, and increased health problems in a herd of cows near a TV and cell phone transmitting antenna over two years. Evaluation of possible factors found no explanation other than high-frequency electromagnetic fields. In an experiment, a cow with abnormal behavior was moved 20 km from the antenna and normalized completely within five days, but symptoms returned when the cow was brought back. The researchers suggested the abnormalities might be related to electromagnetic field exposure, with power densities measured at 0.02-7 mW/m2.
Grigor'ev IuG, Stepanov VS · 1998
Researchers investigated how prior low-level electromagnetic field exposure affects memory formation (imprinting) in chicks. EMF at power densities from 0.4 to 10 mW/cm2 was found to potentially influence the forming of memory (imprinting). The study showed the possibility of EMF-modulated information being fixed in the embryonic brain during the natal period and conserved after birth.
Behari J, Kunjilwar KK, and Pyne S · 1998
Researchers studied the effect of low-level amplitude-modulated radiofrequency radiation on Na+–K+-ATPase activity in the brains of developing male Wistar rats aged 23 days. Rats were exposed to carrier wave frequencies of 147 MHz and its sub-harmonic frequencies 73.5 and 36.75 MHz, amplitude modulated at 16 and 76 Hz, for 30–35 days at 3 hours per day. Na+–K+-ATPase activity increased by around 19–20% in rats exposed with amplitude modulation at 16 Hz, and by around 15–16% in rats exposed with amplitude modulation at 76 Hz, compared to controls. The researchers concluded that the effect on Na+–K+-ATPase activity was insensitive to the carrier wave frequencies under investigation.