Unknown authors · 2024
Researchers exposed Chinese hamster lung fibroblast V79 cells to a 1950 MHz LTE signal at SAR values of 0.3 and 1.25 W/kg. Exposure to the LTE signal alone did not cause any measurable chromosomal damage, oxidative stress, or changes in cell cycle progression. A 20-hour pre-exposure at 1.25 W/kg before mitomycin-C treatment significantly reduced micronuclei, and ROS formation was significantly decreased at both SAR levels. Shorter pre-exposures, lower SAR, or continuous wave exposure did not alter mitomycin-C-induced micronuclei.
Unknown authors · 2024
Researchers evaluated the impact of 5G-modulated 3.5 GHz RF EMF on mitochondrial stress in human fibroblasts and keratinocytes exposed for 24 hours at specific absorption rates of 0.25, 1, and 4 W/kg. They found a statistically significant reduction in mitochondrial ROS in fibroblasts at 1 W/kg, a slight but statistically significant enhancement of UV-B effects in keratinocytes at 0.25 and 1 W/kg, and no effect on mitochondrial membrane potential or apoptosis in any cell type or exposure condition.
Unknown authors · 2024
Gurhan, H., Barnes, F. · 2024
Unknown authors · 2024
Researchers exposed SH-SY5Y neuronal-like cells and peripheral blood mononuclear cells to 2.45 GHz EMR for 24 hours with sulforaphane at 5, 10, and 25 µg/mL. Higher sulforaphane concentrations caused harmful effects and the compound showed a hormetic response in Nrf2, SOD2, and apoptosis markers.
Unknown authors · 2023
Unknown authors · 2023
Exposed MDA-MB-231 cells had higher viability, more live cells, and longer, denser filopodia than unexposed cells. ELF-MF also raised mitochondrial ROS levels and altered mitochondrial morphology in these cells. Proteomic analysis found 328 proteins changed in MDA-MB-231 cells and 242 in MCF-10A cells, with enrichment in focal adhesion and mitochondrion pathways. The exposure reduced adhesion and increased migration and invasion of MDA-MB-231 cells, while transcription factors linked to cellular reprogramming were upregulated in MDA-MB-231 and downregulated in MCF-10A.
Unknown authors · 2023
Researchers exposed semen samples from fertile men to radiofrequency radiation from a smartphone using 4G, 5G, or WiFi connectivity. Sperm motility and viability decreased with WiFi exposure but not with 4G or 5G exposure. The study also tested whether a cellphone case or increased distance could reduce the effects.
Unknown authors · 2023
Researchers analysed DNA methylation profiles of cervical cancer and neuroblastoma cell lines exposed to 900 MHz GSM-modulated radiofrequency electromagnetic fields. Radiofrequency exposure did not affect the DNA methylation of Alu elements in any of the cell lines analysed. It influenced DNA methylation of LINE-1 and ribosomal repeats in terms of average profiles and organisation of methylated and unmethylated CpG sites, in different ways in each of the three cell lines studied.
Unknown authors · 2023
Human keratinocytes were exposed to 900 MHz RF-EMF for 1 hour at a low dose rate (SAR < 10 mW/kg) using a custom system that maintained 37°C, 5% CO2, and 95% humidity. Whole genome bisulfite sequencing was performed directly following exposure to examine immediate changes in DNA methylation patterns and identify early differentially methylated genes. Correlating gene expression with methylation data identified six common targets that were both differentially methylated and differentially expressed, suggesting a potential epigenetic role in the cellular response and that these targets may serve as biomarkers for immediate RF-EMF response.
Unknown authors · 2023
RF and BC induced moderately significant cell damage at 8 or 24 hours and much greater damage after 48 hours and higher BC dose, and an antiproliferative effect increased with exposure time and dose. Oxidative stress and ROS production increased with radiation time, BC dose, and their combination. Both radiation and BC separately and jointly triggered necrosis and apoptosis, with BCL2a gene expression activated after 24 hours and FASR and caspase-3 activation after 48 hours.
Saka VP, Chitra V, Narayanasamy D · 2023
Researchers exposed primary cortical neurons from neonatal rats to electromagnetic radiation from a mobile phone at 2100 MHz and 1.6 W/kg SAR for two hours. The study assessed the induction of apoptosis through modulation of pro- and anti-apoptotic genes via a mitochondrial pathway. Pyrazole derivatives reduced apoptosis by lowering reactive oxygen species and modulating apoptotic gene levels. The compounds showed antioxidative and anti-apoptotic properties.
Unknown authors · 2023
Researchers exposed Chinese hamster ovary cells to a non-toxic dose of 15-nm gold nanoparticles for 48 hours and then to 900 MHz GSM radiofrequency radiation for 24 hours. The conditioned medium from these cells was transferred to new bystander cells, and cell death, DNA breaks, oxidative stress, and COX-2 expression were measured. RFR increased metabolic death in GNP-treated cells, while colony formation was reduced in bystander cells and RFR-exposed cells regardless of GNP presence. ROS levels were significantly lower in GNP-treated cells, but RFR-induced DNA breaks and micronuclei frequencies were not significantly affected by GNP.
Gurhan H, Barnes F · 2023
Gurhan H, Bajtoš M, Barnes F · 2023
Researchers exposed human fibrosarcoma and fibroblast cells to weak RF magnetic fields in the 3–5 MHz range for four days. At 4.2 MHz, exposure significantly increased mitochondrial mass and oxidative stress in fibrosarcoma cells.
Piszczek P et al. · 2022
Unknown authors · 2022
Researchers investigated the effects of 50 and 60 Hz magnetic fields on ROS production, DNA damage, DNA repair, and gene expression in human SH-SY5Y neuroblastoma cells. Cells were exposed to 100 µTRMS magnetic fields for 24 hours, then assayed or treated with menadione before assay. There was suggestive evidence of a low-magnitude increase in ROS-related gene expression immediately after exposure, and 50 Hz fields reduced menadione-induced ROS levels. Magnetic field exposures did not affect cytosolic superoxide levels, DNA damage, or DNA repair rates. No firm evidence was found for differential effects between 50 and 60 Hz fields.
Unknown authors · 2022
Antibacterial activity was measured by reduction in bacterial viable count, and apoptosis in MCF-7 breast cancer cells was assessed using annexin V-FITC and cell cycle analysis. Combined ELF-EMF and 10 µM/ml AgNPs for 2 hours achieved 100% inhibition of K. pneumonia, while either agent alone reduced viable counts in a time-dependent manner. Combination treatment significantly increased apoptosis in MCF-7 cells, shown by elevated Pre G1 phase cells, while AgNPs caused S phase arrest and ELF-EMF caused G2/M phase accumulation.
Unknown authors · 2022
Unknown authors · 2022
Researchers aimed to evaluate the anti-tumor effect of caffeic or chlorogenic acid combined with a moderate-strength static magnetic field on C32 melanoma cells by assessing apoptosis. Apoptosis was evaluated using flow cytometry, apoptosis-associated gene expression was determined using RT-qPCR, and caspase activity and oxidative damage markers were measured. Phenolic acids and a static magnetic field triggered apoptosis of C32 cells and affected expression of genes encoding apoptosis regulatory proteins. The study concluded that both phenolic acids and a static magnetic field can be used supportively in melanoma treatment and that caffeic acid is more pro-apoptotic than chlorogenic acid.
Unknown authors · 2022
The study aimed to examine the 96-hour effects of ELF-EMF on HERV-H, K, and W expression in human melanoma cells. SK-MEL-37 cells were continuously exposed to ELF-EMF at 50 Hz and intensities of 1.5 and 3 mT for 96 hours, and HERV expression was measured by qPCR after mRNA extraction. Exposure to ELF-EMF for 96 hours significantly downregulated HERV-H, K, and W env gene expression (P<0.001). The researchers suggested that low intensity and long-term exposure to ELF-EMF may pave using this type of radiation as a novel therapeutic approach by neutralizing the HERVs upregulated expression in melanoma cells.
Unknown authors · 2022
The results show that extremely low levels of electric stimulation can cause transcriptional changes. The findings reveal the safety of electroporating pulses used in biomedical applications. The results open up the possibility of further therapeutic applications of this technology.
Unknown authors · 2022
The study investigated changes in neuronal activity and synaptic transmission after a 60-minute exposure to 3.0 GHz RF-EMF at a low dose (SAR < 1 W/kg). RF-EMF exposure decreased the amplitude of action potentials, depolarized resting membrane potential, and increased neuronal excitability and synaptic transmission in cultured primary hippocampal neurons.
Unknown authors · 2022
Researchers exposed miR-34a-transfected DLD1 colorectal cancer cells to 2.4 GHz RF-EMF for 24 hours and evaluated effects using real-time PCR, scratch assay, and MTS test. Administration of miR-34a decreased expression of per2, bmal1, sirtuin1, and survivin and inhibited proliferation and migration of DLD1 cells. RF-EMF exposure increased cry1 mRNA expression, weakened miR-34a's inhibition of per2, abolished its inhibition of survivin, and eliminated miR-34a's effects on proliferation and migration. The authors concluded that RF-EMF strongly influenced miR-34a-mediated regulation of the peripheral circadian oscillator in DLD1 cells.
Unknown authors · 2022
The researchers exposed primary human dermal fibroblasts to millimeter waves. The exposure used repeated 60 GHz waves at 2.6 mW cm-2 and 46.8 J cm-2 d-1. After 2 and 4 days, the exposure induced a unique physiological response. High-dose millimeter waves caused non-thermal alterations to the transcriptome and DNA structural dynamics, including G-quadruplex and i-motif formation, but not DNA damage.
