Mahmoudinasab H, Saadat M · 2016
NQO1 mRNA decreased immediately after exposure to the 5 min on/5 min off pattern at 0.25 mT compared to unexposed cells. NQO2 mRNA increased immediately and at two hours after exposure to the same pattern at 0.50 mT compared to unexposed cells.
Unknown authors · 2016
Researchers investigated the effect of a 50 Hz, 1 mT extremely low frequency electromagnetic field on MAP2 and Nestin gene expression in dermal papilla mesenchymal cells. Four experimental groups were prepared: chemical exposure, EMF exposure, combined chemical and EMF exposure, and a control with no treatment, all treated for 5 days. An additional EMF group was exposed for 14 consecutive days to assess the effect of longer exposure on neural differentiation markers Nestin and MAP2. After 14 days, MAP2 expression was higher than after 5 days and cell proliferation decreased from days 5 to 20, indicating that longer exposure promotes neural differentiation.
Akpınar D et al. · 2016
Researchers exposed rats to an electric field at 12 kV/m for 1 hour per day, dividing them into control, prenatal, postnatal, and prenatal-plus-postnatal groups. The mismatch negativity amplitude was decreased in all exposed groups compared with the control group. Levels of 4-HNE, a marker of lipid peroxidation, were significantly increased in the postnatal group compared with the control group, while protein carbonyl levels were significantly decreased in the prenatal-plus-postnatal group compared with the control, prenatal, and postnatal groups.
Unknown authors · 2016
Researchers exposed rats to 40 Hz electromagnetic fields (similar to power line frequencies) daily for three weeks and tracked changes in basic behaviors like eating, drinking, movement, and blood sugar. The exposed rats showed disrupted weight patterns, reduced appetite, altered glucose levels, and decreased activity on the first day after exposure. These findings suggest that even extremely low-frequency EMF can disrupt normal biological functions in mammals.
Deshmukh PS et al. · 2016
Researchers exposed male Fischer rats to microwave radiation for 90 days at 900, 1800, and 2450 MHz to investigate effects on cognitive function, heat shock protein 70 (HSP70) levels, and DNA damage in the brain. Cognitive function was tested using the elevated plus maze and Morris water maze, HSP70 levels were measured by ELISA, and DNA damage was assessed using alkaline comet assay. The results suggest that low-level microwave exposure at these frequencies may lead to hazardous effects on the brain.
Ansari AM et al. · 2016
Researchers exposed male adult NMRI mice to an extremely low frequency magnetic field at 0.5 mT and 50 Hz, either as a single 2-hour session or for 2 hours a day over 2 weeks. Depressive-like behavior was measured using the Forced Swimming Test, which records immobility time. Long-term exposure significantly reduced immobility time, while short-term exposure did not alter it.
Feng B, Ye C, Qiu L, Chen L, Fu Y, Sun W · 2016
Human amniotic FL cells were exposed to a 50-Hz magnetic field for different durations or intensities, then incubated with staurosporine for 4 hours. Magnetic field exposure alone had no significant effect on early apoptosis or cell viability. When cells were also treated with staurosporine, magnetic field exposure had a protective effect against early apoptosis, and this effect could be abolished by MPT inhibitors. The anti-apoptotic effect was completely eliminated when Akt was inhibited, suggesting that Akt activation was necessary for the effect.
Hu Y et al. · 2016
Mahmoudinasab H, Sanie-Jahromi F, Saadat M · 2016
Researchers designed the study to evaluate the effect of EMFs on the mRNA levels of seven antioxidant genes in human MCF-7 cells. Three exposure patterns were tested at two field intensities, 0.25 mT and 0.50 mT, each with a total exposure time of 30 minutes. The MTT assay showed no cytotoxic effect on MCF-7 cells under any of the three exposure conditions at either field intensity. Under the 15-min-on/15-min-off pattern, the 0.50 mT intensity was associated with more up- or down-regulated genes than 0.25 mT, a statistically significant difference (P=0.041), while no such difference was found for the other two exposure patterns.
Martínez MA, Úbeda A, Moreno J, Trillo MÁ · 2016
Researchers investigated how power frequency magnetic fields affect cell proliferation in NB69 cells and the role of p38 and JNK kinases and reactive oxygen species in that response. NB69 cultures were exposed to a 100 µT magnetic field, either intermittently for 24, 42 or 63 hours, or continuously for periods of 15 to 120 minutes, with or without p38 or JNK inhibitors and an antioxidant. Field exposure induced transient activation of p38, JNK, and ERK1/2, but only the p38 inhibitor blocked the proliferative effect. The antioxidant NAC blocked the field effects on cell proliferation and p38 activation, but not those on ERK1/2 activation.
Yin C, Luo X, Duan Y, Duan W, Zhang H, He Y, Sun G, Sun X · 2016
Researchers investigated whether lotus seedpod procyanidins (LSPCs) could protect primary cultured rat hippocampal neurons from extremely low frequency electromagnetic field (ELF-EMF)-induced neurotoxicity. ELF-EMF exposure significantly decreased cell viability and increased the number of apoptotic cells compared with control. LSPCs evidently protected the hippocampal neurons against ELF-EMF-induced cell damage, and the researchers concluded this occurred through inhibiting oxidative stress and a mitochondrial apoptotic pathway.
Ansari AM et al. · 2016
Researchers exposed mice to extremely low frequency magnetic fields (the type emitted by power lines and electrical devices) for either 2 hours once or 2 hours daily for 2 weeks. They found that long-term exposure reduced depression-like behavior in the mice, while short-term exposure interfered with antidepressant medications. This suggests that chronic EMF exposure may alter brain chemistry and affect how psychiatric medications work.
Madjid Ansari A et al. · 2016
Researchers exposed mice to extremely low frequency magnetic fields (the type generated by power lines and electrical appliances) to study effects on depression-like behavior. They found that short-term exposure (2 hours) had no effect, but long-term exposure (2 hours daily for 2 weeks) actually reduced depressive symptoms in the mice. The study suggests this effect may work through changes in nitric oxide levels in the brain.
Maes A, Anthonissen R, Wambacq S, Simons K, Verschaeve L. · 2016
Researchers conducted an in vitro cytogenetic laboratory investigation to explore a possible association between extremely low frequency magnetic fields and Alzheimer's disease risk. The study found that 50 Hz ELF-MF increased the frequency of cells with large micronuclei and nuclear buds, suggesting that fields above 50 μT may induce chromosome instabilities like those found in Alzheimer's disease patients. The researchers noted that results from other published experimental studies on ELF-MF and Alzheimer's disease are described as rather reassuring.
Shahbazi-Gahrouei D, Shiri L, Alaei H, Naghdi N. · 2016
Researchers investigated the effect of extremely low frequency magnetic fields (ELF-MFs) at 10 Hz and 690–720 μT on the level of 5-hydroxyindolacetic acid (5-HIAA) in adult male Wistar rats. A total of 24 adult Wistar male rats were exposed to the magnetic field for 15 successive days, after which 5-HIAA was measured from dialysate samples using high performance liquid chromatography. Exposure for 3 hours daily decreased 5-HIAA in the raphe nucleus, but exposure for 1 hour daily was not effective in altering the level of 5-HIAA.
Rostami A et al. · 2016
Researchers exposed male rats to 3 Hz and 60 Hz ELF/EMF using a protocol of two cycles of 2 hours per day for four days with a two-day interval. Immediately after exposure, locomotor activity was significantly lower in both exposed groups compared with sham-exposed rats. The exposure did not change anxiety-like behavior or short-term memory over the following two weeks. Electrophysiological recording showed a significant reduction in locus coeruleus firing rate after two hours of exposure. Proteomic analysis indicated global changes in the whole brain proteome after treatment.
Reale M et al. · 2016
Researchers exposed SH-SY5Y neuroblastoma cells to 50 Hz/1 mT ELF-EMF for short (1h-3h) and sub-chronic (48 h) periods. Exposed cells showed an increased intracellular 5-hydroxyindoleacetic acid/5-hydroxytryptamine ratio reflecting transmitter synthesis, catabolism, and release. Matrix metalloproteinases involved in neuronal cell death were not significantly altered. The balance between reactive oxygen species generation and elimination, and between pro- and anti-inflammatory cytokines, was maintained. The changes were minor compared to damage expected to underlie neurodegeneration or cognitive impairment.
D'Angelo C et al · 2015
Researchers studied MCP-1 expression in HaCaT, SH-SY5Y, THP-1, and K562 cells exposed to a sinusoidal EMF at 50 Hz with a flux density of 1 mT (rms). Preliminary results showed that EMF exposure differently modifies MCP-1 expression in different cell types. The authors state that MCP-1 expression needs to be better determined with additional studies using different parameters and exposure times.
D'Angelo C et al · 2015
Italian researchers exposed four different types of human cells to 50 Hz electromagnetic fields (the same frequency as power lines) at 1 milliTesla strength. They found that this exposure changed the production of MCP-1, a protein involved in immune response and inflammation, but the effects varied depending on the cell type. This suggests that power-frequency EMFs can trigger biological responses in human cells, though more research is needed to understand the health implications.
Megha et al. · 2015
Researchers exposed 24 male Fischer 344 rats to microwave radiation at 900, 1800, and 2450 MHz with specific absorption rates of 0.59, 0.58, and 0.66 mW/kg for 60 days (2 hours per day, 5 days per week). Exposure increased oxidative stress markers malondialdehyde, protein carbonyl, and catalase, while decreasing reduced glutathione and superoxide dismutase. Pro-inflammatory cytokines IL-2, IL-6, TNF-α, and IFN-γ were significantly elevated in exposed rats. Significant DNA damage was observed in the microwave exposed groups compared to sham.
Unknown authors · 2015
Researchers exposed rats to low-intensity microwave radiation at cell phone frequencies (900, 1800, and 2450 MHz) for 60 days and found significant brain damage. The study revealed increased oxidative stress, inflammation, and DNA damage in the hippocampus, with effects becoming more severe at higher frequencies. This suggests that even very low power microwave exposure can harm brain tissue through multiple biological pathways.
Çiğ B, Nazıroğlu M. · 2015
Researchers exposed MCF-7 breast cancer cells to 900, 1800, and 2450 MHz electromagnetic radiation at distances from 0 to 25 cm for one hour. At distances of 0, 1, and 5 cm, exposed cells showed higher reactive oxygen species, calcium concentrations, apoptosis, and caspase activity compared to controls, while cell viability increased with distance. No statistically significant differences were observed between control cells and those exposed at 20 or 25 cm.
Çiğ B, Nazıroğlu M · 2015
Researchers exposed breast cancer cells to radiation from mobile phones (900 MHz, 1800 MHz) and Wi-Fi (2450 MHz) at various distances for one hour. They found that EMF exposure within 10 cm triggered harmful cellular effects including oxidative stress, calcium overload, and programmed cell death, while distances beyond 10 cm showed no significant effects. This suggests maintaining distance from wireless devices may reduce biological harm.
Hardell & Carlberg (2015) Increasing rates of brain tumours in the Swedish National Inpatient Register & the Causes of Death Register. Int J Envir Res Public Health. http://bit.ly/1aDHJm Devocht (2016) Inferring the 1985–2014 impact of mobile phone use on selected brain cancer subtypes using Bayesian structural time series and synthetic controls. Environ Int. http://bit.ly/2jJlbZu corrigendum (2017): http://bit.ly/2Cuq2nU Hardell & Carlberg (2017) Mobile phones et al. · 2015
Shokri S, Soltani A, Kazemi M, Sardari D, Mofrad FB · 2015
Iranian researchers exposed male rats to 2.45 GHz Wi-Fi radiation (the same frequency as most home routers) for either 1 or 7 hours daily over two months. Both exposure groups showed decreased sperm quality, increased cell death in the testes, and reduced seminal vesicle weight compared to unexposed controls. The damage was worse with longer daily exposure times.