Syed Taha SMA et al. · 2025
Male Sprague Dawley rats (n = 6 per group) were exposed for 60 days to 3.5 GHz or 24 GHz RF-EMF for 1 h/day or 7 h/day, and testicular cytokines, apoptosis-related genes, and sperm quality were evaluated. IL-10 was significantly reduced in the 24 GHz group at both 1-h and 7-h exposure duration, and at 7 h TNF-α was also lower at 24 GHz. Casp3 expression was higher and Tp53 was lower at 3.5 GHz at 1-h exposure duration. Sperm concentration and viability were reduced after 24 GHz exposure at 7 h, while sperm motility was reduced after 3.5 GHz exposure at both durations.
Unknown authors · 2025
Researchers exposed mice to a 5G-modulated 3.5 GHz signal for 1 hour daily, 5 days per week over six weeks at a brain-averaged SAR of 0.19 W/kg. Behavioral tests after four weeks showed no significant effects on locomotion, anxiety, or memory. mRNA profiling at the end revealed significant expression changes in less than 1% of genes, with over-representation of glutamatergic synapse genes. The right cortical area with a SAR of 0.43 W/kg showed more mitochondrial genome-encoded responsive genes than the left area with a SAR of 0.14 W/kg.
Ilgaz NS et al. · 2025
After exposure, liver tissues were examined for DNA damage using the Comet assay and for histopathologic changes using Masson Trichrome and Hematoxylin Eosin staining. The Comet assay showed higher genetic damage index and damaged cell percentage in exposed rats, but the difference was not statistically significant. Histopathologic examination revealed more prominent portal inflammation, single cell necrosis, vascularity, and congestion in the exposed group.
Gelenli Dolanbay E et al. · 2025
Pregnant Wistar Hannover rats were divided into sham control, full-gestation exposure (3T RFR), and late-gestation exposure (2T RFR) groups with 2 hours of exposure per day. Male offspring were euthanized at 12 months for testicular analysis. In the full-gestation exposure group, seminiferous tubule diameter and epithelial height were significantly reduced compared to controls. Abnormal sperm morphology increased significantly in the full-gestation exposure group. DNA damage markers and apoptosis were elevated in both exposure groups compared to sham.
Unknown authors · 2025
Yilmaz H, Tümkaya L, Mercantepe T, Yılmaz A, Gül F, Suzan ZT · 2025
Researchers exposed male Sprague-Dawley rats to 5.9 GHz electromagnetic fields for two hours daily over 30 days. The EMF exposure caused histopathological damage including edema, vascular congestion, inflammation, and loss of sperm cells. EMF exposure also increased oxidative stress markers and endoplasmic reticulum stress proteins in testicular tissue. Coenzyme Q10 treatment reversed both the histopathological and biochemical changes caused by EMF exposure.
Unknown authors · 2025
Researchers used 32 Wistar Albino rats divided into four groups: sham, MIR, prophylactic RF-EMF plus MIR, and therapeutic RF-EMF plus MIR. After the experiment, blood and terminal ileum samples were collected for histopathological, immunohistochemical, genetic, and biochemical analyses. In the MIR group, expressions of Cas-3, TNF-α, VEGF, BAX, and HIF-1α increased, while OSI levels and PCNA, BCL2, and eNOS expressions decreased. Marked hyperemia, hemorrhage, edema, inflammatory cell infiltrations, and erosion or ulcers were observed in the MIR group. Both prophylactic and therapeutic RF-EMF treatment reversed these molecular and pathological changes, with therapeutic treatment showing more effective recovery.
Unknown authors · 2025
Researchers exposed female Wistar rats to 700MHz radiation for short-term (6 hours daily for 10 days) and long-term (4 hours daily for 60 days) periods. No changes were seen in estrous cycles or DNA damage in either exposure group. Testosterone levels increased significantly in exposed rats, while estradiol and progesterone remained normal. Exposed ovaries showed elevated malondialdehyde and reduced superoxide dismutase, total sulfhydryl content, and ferric reducing antioxidant power. Histopathology revealed no significant ovarian morphology changes after short-term exposure but cystic follicles and abnormal vasculature after long-term exposure.
Hancı H, Yenilmez E, Demir S, Yıldırım M, Gedikli Ö, Kaya H · 2025
Nine pregnant Sprague Dawley rats were assigned to three groups, with the control group receiving no procedure. The sham group was placed in the EMF cage for 1 hour daily during pregnancy without exposure, and the EMF group was exposed to 900 MHz EMF for the same duration. Offspring were evaluated on postnatal days 21 and 60 with open field and rotarod tests, followed by electrophysiological measurements, and sciatic nerves were analyzed histopathologically, histomorphometrically, immunohistochemically, and biochemically. The study concluded that prenatal 900 MHz EMF exposure adversely affected peripheral nerve development, with persistent morphological changes in the sciatic nerve into adulthood that were not severe enough to impair functional outcomes.
Günay S, Delen K, Özkan ET, Kuzay Aksoy D, Sırav Aral B · 2025
Gautam R, Jha N, Tomar AK, Nirala JP, Arora T, Rajamani P · 2025
The exposed group received 35.5 GHz millimeter wave radiation for 2 hours per day over 60 days. Exposure significantly reduced sperm viability and count and caused morphometric changes in testicular tissue. Lipid peroxidation increased while superoxide dismutase, total sulfhydryl levels, and total antioxidant capacity decreased in exposed rats. DNA damage measured by comet assay was significantly increased in the exposed group.
Er H, Basaranlar G., Derin N., Kantar D, Ozen S. · 2025
Researchers exposed adult rats to 2100 MHz RFR for 2 hours per day, 5 days per week, for either 1 week or 10 weeks, with sham controls. In the 1-week exposure group, all auditory brainstem response wave latencies were prolonged, oxidative stress markers increased, and antioxidant enzyme activities decreased. Acute exposure also caused edema in neurons, astrocytes, and astrocytic end-feet, as well as mitochondrial damage in astrocytes. The 10-week exposure group showed no harmful effects on the auditory system.
Bektas H, Bese Akgun BB, Cakir S, Dogu S, Ahnas B · 2025
Altun G, Kaplan S · 2025
Offspring hypothalamic neurons were analyzed using stereological, immunohistochemical, and ultrastructural methods, and anxiety and locomotor behaviors were assessed. EMF exposure reduced neuron numbers in the arcuate nucleus and increased anxiety-like behavior in offspring. Oxidative stress markers were altered and NPY immunoreactivity was stronger in EMF-exposed offspring, while melatonin and omega-3 did not show clear therapeutic effects.
Unknown authors · 2025
Forty male Wistar rats were randomly divided into four groups (Sham, IR, IR+RF-EMF, RF-EMF), and IR was applied by 60 minutes of left lung hilus clamping followed by 60 minutes of reperfusion, while all rats were kept in the RF-EMF unit for 60 minutes with or without activation. IR injury increased emphysematous findings, hyperemia, septal thickness, TNF-alpha and caspase-3 expression, and raised total oxidant status, oxidative stress index, HIF-1α, eNOS, and BAX while decreasing BCL2. In the IR+RF-EMF group, improvement was detected in all parameters, indicating antioxidant, antiapoptotic, and anti-inflammatory effects through the HIF-1α/eNOS and BCL-2/BAX pathways.
Unknown authors · 2024
After four weeks the offspring were sacrificed and hippocampal, kidney, and liver tissues were examined for injury. EMF exposure increased necrotic areas in the hippocampus, hyperemia and necrosis in the kidneys, and degeneration in the liver compared with the group that received both EMF and curcumin. Daily curcumin during pregnancy prevented these tissue injuries in the fetuses.
Sissons SM, Dotta BT · 2024
Researchers exposed perinatal rats to tap water, L-arginine, or N-methylarginine, with half of each group also receiving a 7-Hz square-wave EMF at 5, 50, or 500 nT for 2 to 14 days after birth. Brains were harvested in adulthood at a mean age of 568.17 days and stained with Toluidine Blue O for neuronal counts in the hippocampus, cortices, amygdala, and hypothalamus. Histological analysis revealed a main EMF effect of increased neuronal counts in the secondary somatosensory cortex at low intensities, a significant increase in the left CA3 region in exposed females, and a significant increase in the right entorhinal cortex in exposed males.
Klimek A et al. · 2024
Researchers exposed rats to 50 Hz ELF-EMF at 1 and 7 mT for 1 hour per day over 7 days, repeated the procedure three times, and measured adrenaline, noradrenaline, MHPG, and β2-adrenergic receptor expression. After each exposure, some rats underwent a behavioural test to assess stress response to subsequent stress factors. ELF-EMF at 1 mT induced adaptive changes in the noradrenergic locus coeruleus system. Exposure to 7 mT increased stress system activity, causing sensitization to a different stress factor, profoundly decreased β2-adrenergic receptor levels, and moderately increased anxiety-related behaviour.
Unknown authors · 2024
Researchers investigated the permeability, fine structure, and protein expression of tight junctions in the blood-brain barrier of rats exposed to electromagnetic pulses. They used fluorescein isothiocyanate-labeled dextran of different molecular masses to measure tracer leakage under varying field intensities. Transmission electron microscopy revealed open tight junctions in endothelial cells of exposed rats compared to sham-irradiated controls. Western blot analysis showed no change in levels of ZO-1, claudin-5, or occludin proteins.
Unknown authors · 2024
Researchers exposed mouse zygotes to cell phone electromagnetic radiation at 900-1800 MHz for 30 minutes on day 1. Exposed embryos showed slower cleavage divisions, more abnormal cleavage patterns, and delayed blastocyst formation. Cell survival in blastocysts was lower in the exposed group.
Mahmoudi R et al. · 2024
Researchers divided 32 adult male Wistar rats into four groups: control, RF-EMF alone, heat stress alone, and RF-EMF combined with heat stress. Rats in the RF-EMF group were exposed for 2 hours daily over 52 days, while the heat stress group received 10 minutes of heat per day for the same period. After 52 days, the combined RF-EMF and heat group showed significant improvements in testis volume, tubular epithelium, interstitium, cell counts, sperm quality, and Leydig cells compared with the heat-only group.
Unknown authors · 2024
Karamazı Y, Emre M, Uçar S, Aksoy G, Emre T, Tokuş M · 2024
The study used 20 female and 20 male Wistar Albino rats divided into four groups with different exposure patterns to 6 GHz RF-EMF at 0.054 W/kg. Rats were exposed for 4 hours per day for 6 weeks before mating, and pregnant rats in Group-II and Group-III were exposed for 4 hours per day for 18 days of gestation. Fetal weight, body lengths, and bone measurements all increased significantly in every exposure group compared with controls.
Zhao J, Ma J, Wang X, Zhang B · 2024
Researchers exposed mice to 90 kHz electromagnetic fields from a WPT biosafety experimental platform for 2, 4, or 8 weeks. They tested learning and memory using the Novel Object Recognition test and measured neuronal excitability with whole-cell patch-clamp recordings. Long-term exposure accelerated action potential release, inhibited potassium channel activation, and increased dentate gyrus neuron excitability, but did not significantly affect cognitive function.
Unknown authors · 2024
Researchers created two groups of seven rats each, a sham control and an exposure group, and exposed the latter to 2100 MHz RF-EMF for five hours per day over 14 days. Histological examination of brain tissue from the exposed group revealed vascular alterations and significant edema. The comet assay showed much more cellular damage in the exposed group (18.26% tail DNA) than in controls (4.06% tail DNA). Testicular tissue showed significant deterioration of spermatogenic cells in the exposed group. Bax and bcl-2 mRNA levels differed significantly between groups, while p53 did not change.
