Lundberg L, Sienkiewicz Z, Anthony DC, Broom KA. · 2019
Researchers exposed male C57BL/6J mice for 30 min to 50 Hz magnetic fields at 580 μT, blue LED light at 700 lux, both, or neither, to test whether power frequency magnetic fields can influence circadian rhythm control. The magnetic fields did not modulate the response to light and had no significant effect on adrenal markers on their own, though they were associated with a decrease in locomotor activity (P < 0.03). Gene expression of cry1 and cry2 in the adrenals, liver, and hippocampus was not affected by any of the exposures (P > 0.05).
Li Y, Zhang Y, Wang W, Zhang Y, Yu Y, Cheing GL, Pan W. · 2019
Researchers allocated forty Sprague-Dawley rats to four groups, including an STZ-induced dementia group exposed to pulsed EMF at 10 mT and 20 Hz. The Morris water maze was used to assess learning and memory abilities, and IGF-1 and IGF-2 gene expression were measured by quantitative PCR. Mean escape latency was reduced by 66%, swimming distance decreased by 55%, and time for first crossing the platform decreased by 41.6% in the pulsed EMF group compared with the STZ group. IGF-2 gene expression significantly increased in the pulsed EMF group compared with the STZ group.
Di G, Kim H, Xu Y, Kim J, Gu X. · 2019
Researchers exposed mice to a static electric field (SEF) and a power frequency electric field (PFEF), each at 35 kV/m, for 49 days. The study found that a 35 kV/m SEF did not cause significant influences on learning and memory ability in mice, while a 35 kV/m PFEF caused significant positive effects on learning and memory ability on day 33. Behaviors were assessed using the Morris water maze test, and amino acid neurotransmitter levels in the hippocampus were examined during exposure.
Alkis ME et al. · 2019
Researchers exposed male Sprague Dawley rats to radiofrequency radiation at 900, 1800, or 2100 MHz for 2 hours per day for 6 months to study DNA damage and oxidative stress in blood and brain tissue. DNA damage and oxidative stress indicators were found higher in the radiofrequency radiation exposure groups than in the sham-control group. The researchers concluded that 900-, 1800-, and 2100-MHz radiofrequency radiation may cause oxidative damage, induce an increase in lipid peroxidation, and increase oxidative DNA damage in the frontal lobe of rat brain tissues, and that 2100-MHz radiation may cause formation of DNA single-strand breaks.
Unknown authors · 2018
Psychomotility was assessed in an open field, attention in novel object recognition, and spatial learning in the Morris water maze. EMF stimulation increased vertical rearing activity in the open field, improved attention-based discrimination in novel object recognition, and facilitated working memory spatial learning in the water maze. No undesirable side effects were observed even at the highest dose.
Unknown authors · 2018
The study investigated the effects of low-frequency PEMF pretreatment on bone marrow-derived mesenchymal stem cells and their ability to promote regeneration of a crush-injured rat mental nerve using in vitro and in vivo experiments. In vitro, PEMF-pretreated BMSCs proliferated faster and showed greater mRNA expression of growth factors compared with unpretreated BMSCs. In vivo, rats receiving PEMF-pretreated BMSCs had higher myelinated axon counts, greater axon density, more DiI-labeled neurons in the trigeminal ganglia, and faster functional recovery than those receiving unpretreated BMSCs.
Unknown authors · 2018
Researchers exposed 80 male rats to extremely low frequency (50 Hz) electromagnetic fields at magnetic flux intensities of 1, 100, 500, and 2,000 μT and a control. All rats were immunized with human serum albumin after 1 month of exposure and the experiment continued for another 1 month. Thymus weight significantly declined at intensity of 2,000 μT. At the preimmunization phase, serum IL-17 and TGF-β levels were significantly decreased at intensities of 1 and 100 μT. Foxp3 expression was also downregulated at intensities of 1 and 100 μT.
Unknown authors · 2018
Unknown authors · 2018
Researchers exposed 5xFAD mice to 1950 MHz RF-EMF at a specific absorption rate of 5.0 W/kg for 2 h/day and 5 days/week for 8 months. Behavioral changes were assessed using an open field test and an object recognition memory task after exposure ended. RF exposure rescued hyperactivity-like and anxiolytic behaviors in the open field test and improved cognitive deficits in the object recognition memory test. Glucose metabolism in the hippocampus and amygdala was significantly increased in RF-exposed mice compared to sham-exposed mice.
Keleş AI et al. · 2018
Histopathological examination revealed impairments of pyramidal and granular cell structures in the hippocampus of exposed rats. No significant differences were found in learning, memory, or locomotor behavior among the groups.
Qin F et al. · 2018
Researchers exposed male mice to 1800 MHz radiofrequency fields at 40 μW/cm2 and 0.0553 W/Kg SAR for 2 hours per day over 32 days. The exposure significantly reduced testosterone levels and altered the expression of steroidogenic genes Star, P450scc, P450c17, and 3β-Hsd in testicular tissue. RF exposure also significantly reduced the regulatory protein CaMKI/RORα, and similar effects were observed in cultured primary Leydig cells exposed in vitro. The CaMK inhibitor KN-93 blocked all these observations, and ionomycin reversed the RF-induced down-regulation of intracellular calcium and CaMKI/RORα expression.
Unknown authors · 2018
Recent research focuses on unraveling mechanisms of radiofrequency electromagnetic radiation and potential cellular targets. Omics high-throughput approaches are powerful tools to investigate global effects of radiofrequency electromagnetic radiation on cellular physiology.
Unknown authors · 2018
Researchers exposed female Wistar albino rats to 1800 MHz RF-EMF at 6.8 ± 0.1 V/m and 0.06 W/kg SAR for 2 hours per day for eight weeks, with a sham group kept under similar conditions without exposure. They measured Hsp27, p38MAPK, EGFR, and caspase-3 gene expression using real-time PCR. Caspase-3 and p38MAPK gene expression were significantly upregulated in the ocular tissues of exposed rats.
Unknown authors · 2018
Twelve-week old male mice were exposed to 2.45 GHz microwave radiation at 0.0248 mW/cm2 power density and 0.0146 W/kg SAR for two hours daily over 15, 30, and 60 days. Researchers evaluated testicular histology, serum testosterone, ROS, NO, MDA, antioxidant enzymes, and apoptotic protein expression. Exposure induced testicular redox imbalance that enhanced apoptosis via a p53 dependent Bax-caspase-3 pathway with severity increasing with exposure duration.
Unknown authors · 2018
Researchers divided male Wistar-Albino rats into four groups of eight and exposed one group to 2600 MHz EMF for 30 days. EMF exposure increased sinus dilatation, caspase-3 and TNF-α positive cells, and malondialdehyde levels in liver tissue. Quercetin at 100 mg/kg/day did not prevent the liver damage.
Unknown authors · 2018
Researchers exposed female rats to a continuous 900-MHz electromagnetic field for one hour daily from postnatal day 35 to 59. Control, sham, and EMF groups were established, with sham rats receiving a pseudo-megahertz effect. All animals were euthanized on postnatal day 60 and kidney tissues were analyzed for histopathology and oxidative stress markers. Histopathological analysis showed no pathology in control or sham groups, but the EMF group exhibited hemorrhage in glomeruli, vacuolization and irregularity in tubular epithelium, glomerular degeneration and edema, Bowman capsule degeneration, medullary hemorrhage, disturbed nuclei, and cortical tubular edema. Total oxidant status and oxidative stress index values were significantly lower in both EMF and sham groups compared with controls.
Unknown authors · 2018
Male albino Wistar rats were exposed to 900 MHz radiation for 1 hour per day at a power density of 146.60 µW/cm2 from a mobile phone for 28 days. MDA concentration was increased but TA levels and GST activity were not different in the exposed group compared to controls. Sperm motility was slightly reduced and percentage of abnormal sperm was significantly elevated in the exposed group. Loss of germ cells, particularly spermatocytes and spermatids, was found in the testes of exposed rats, and testes caspase-3 activity was slightly elevated.
Unknown authors · 2018
Mice were exposed to 2.45 GHz microwave radiation at a power density of 0.25 mW/cm2 and SAR of 0.09 W/kg for 2 hours per day over 30 days. Exposure significantly increased catalase, malondialdehyde, and reactive oxygen species while decreasing glutathione peroxidase, testosterone, and superoxide dismutase levels. Flow cytometry revealed micronuclei formation in the blood of exposed mice, and histopathological changes were observed in seminiferous tubules.
Unknown authors · 2018
Researchers randomly assigned rats to cage control, sham, or 2.1 GHz RF exposure for 2 hours daily over 7 days and recorded auditory evoked potentials. Peak-to-peak amplitudes of auditory evoked potentials were significantly higher in the RF group than in the sham group. Evoked power, inter-trial phase synchronization, and auditory evoked gamma responses were also significantly higher in the RF group. The RF group showed significantly lower TBARS and 4-HNE levels, while GFAP, nNOS, and iNOS levels did not differ between groups, and no differences were found between cage control and RF groups.
Hancı H, Kerimoğlu G, Mercantepe T, Odacı E · 2018
Twenty-four Sprague Dawley rats aged 21 days were randomly divided into three groups, and the EMF group rats were exposed to continuous 900-MHz EMF for 1 h each day during adolescence. The results showed that changes may occur in morphology and oxidative stress biomarkers in the rat testis after such exposure.
Unknown authors · 2018
They measured reduced glutathione (GSH) and malondialdehyde (MDA) in kidney tissue and used caspase-3 immunohistochemistry to assess apoptosis. Deterioration was observed in the brush border in renal tubules of the EMF groups. The immunohistochemical analysis revealed a greater number of positively stained renal tubular epithelial cells in the EMF groups compared with the control group. In the EMF groups renal MDA levels increased and renal GSH levels decreased compared with the control group (p = 0.00 for both).
Unknown authors · 2018
Twenty-four 34-day-old female Sprague-Dawley rats were divided into control, sham, and EMF exposure groups. The EMF group received 900-MHz electromagnetic field exposure for 1 hour daily from postnatal days 35 to 59. Ovarian tissue in the EMF group showed thinning of granulosa and theca layers, shrunken granulosa cells, reduced mitotic activity, and leukocyte infiltration. Secondary follicle counts were significantly lower in the EMF group than in the control or sham groups. Biochemical analysis found increased oxidative stress markers and antioxidant enzymes in both EMF and sham groups compared to controls, with the EMF group showing the highest total oxidant status and oxidative stress index.
Jeong YJ et al. · 2018
The aim of the study was to observe the impact of long-term RF-EMF on oxidative stress and neuroinflammation in aging brains of C57BL/6 mice. Compared with young mice, aged mice showed significantly increased protein and lipid oxidative damage markers in the brain. Aged mice also had significantly elevated markers of DNA damage, apoptosis, astrocytes, and microglia. Long-term RF-EMF exposure did not change the levels of oxidative stress, DNA damage, apoptosis, astrocyte, or microglia markers in aged mouse brains. Long-term RF-EMF exposure also did not alter locomotor activity in aged mice.
Esmaeilpour K et al. · 2018
Hippocampal kindled rats showed deficits in learning and memory in the passive-avoidance test. Low-frequency stimulation reversed the impairment in learning and memory behavior in kindled rats. Low-frequency stimulation also diminished kindling-induced neuronal loss and atrophy in the hippocampus. The stimulation protocol used 200 monophasic square wave pulses of 0.1 ms duration at 1 Hz, delivered in four packages every five minutes.
Pandey N, Giri S. · 2018
Researchers exposed Swiss albino mice to RFR for 3 hours twice daily for 35 days and gave some mice melatonin at 5 mg/kg body weight per day. RFR-exposed mice showed extensive DNA damage, arrest in pre-meiotic spermatogenesis, low sperm count, and sperm head abnormalities. Biochemical assays revealed excess free radical generation and histological changes in testis and germ cells. Melatonin supplementation diminished or abolished these effects.
