Kimsa-Dudek M et al. · 2018
Researchers evaluated how simultaneous exposure to fluoride and a static magnetic field from permanent magnets affects the expression of antioxidant defense genes in human fibroblasts. They used control fibroblasts and fluoride-treated fibroblasts exposed to a static magnetic field with moderate induction, analyzing gene expression with an oligonucleotide microarray. Fluoride ions caused oxidative stress in the cells. Exposure to a static magnetic field with moderate induction normalized the expression of five antioxidant defense genes (SOD1, PLK3, CLN8, XPA, HAO1) that were altered by fluoride.
Gupta SK, Mesharam MK, Krishnamurthy S. · 2018
Male rats were exposed to EMR (900, 1800 and 2450 MHz) every day for 1 h for 28 consecutive days. Animals exposed to EMR-2450 MHz exhibited significant cognitive deficits. EMR-2450 MHz caused loss of mitochondrial function and integrity, an increase in amyloid beta expression. There was release of cytochrome-c and activation of apoptotic factors such as caspase-9 and -3 in the hippocampus. Further, there was decrease in levels of acetylcholine, and increase in activity of acetyl cholinesterase, indicating impairment of cholinergic system.
Ruigrok HJ et al. · 2018
Researchers monitored TRPV1 activation in real time on live cells using a bioluminescence resonance energy transfer technique. Transiently-transfected HEK293T cells were exposed to 1800 MHz radiofrequency signals including CW, GSM, UMTS, LTE, Wi-Fi and WiMAX at specific absorption rates between 8 and 32 W/kg. TRPV1 channels were activated by the heat produced by radiofrequency exposure, but no activation occurred without temperature elevation. Radiofrequency exposure did not alter the maximal efficacy of capsaicin to activate TRPV1 at fixed temperature.
Qureshi MRA, Alfadhl Y, Chen X, Peyman A, Maslanyj M, Mann S · 2018
Lasalvia M et al. · 2018
Researchers exposed human peripheral blood lympho-monocytes in a reverberation chamber to 1.8 GHz electromagnetic fields at 200 V/m for times ranging from 1 to 20 hours. Some exposed cells took on an enlarged and deformed shape, and Raman spectra of the nuclear compartment showed reductions in DNA backbone-linked vibrational modes. After 20 hours of exposure, resting oxygen consumption rate increased and was coupled to a significant increase of the FoF1-ATP synthase-related oxygen consumption. The researchers suggested that adaptive mechanisms, likely via redox signaling, may compensate for early impairments of the oxidative phosphorylation system caused by EMF exposure.
Zuo H, Liu X, Wang D, Li Y, Xu X, Peng R, Song T. · 2018
Researchers created an Alzheimer's disease rat model using D-galactose and Aβ25-35 injections, then exposed the animals to 50 Hz and 400 µT ELF-MF for 60 continuous days. ELF-MF exposure partially improved the cognitive disorder and upregulated the levels of RKIP, TAK1, and the RKIP/TAK1 interaction, but downregulated p-IKK levels in AD rats. The researchers suggested that ELF-MF exposure might have a potential therapeutic value for AD.
Laszlo AM et al. · 2018
Researchers exposed turkeys to extremely low frequency electromagnetic fields (50 Hz; 10 μT) for 3 weeks and then observed the birds for a 5-week regeneration period without exposure. Norepinephrine-activated β-adrenoceptor function decreased in the treated birds in a time-dependent manner, while toxicological parameters in the serum showed no differences compared to normal ranges. The decreased β-adrenoceptor function was compensated by the homeostatic complex during the 5-wk regeneration period.
Kazemi M et al. · 2018
Researchers exposed male rhesus macaques to extremely low frequency magnetic fields at 0.7 μT for 4 hours per day over 30 consecutive days, at either 1 Hz or 12 Hz. Visual working memory scores and plasma melatonin levels increased significantly in the animals exposed to 12 Hz, but not in those exposed to 1 Hz. NMDA receptor gene expression also increased at 12 Hz exposure, while hippocampal volume did not increase significantly at either frequency.
Hong I et al. · 2018
Researchers delivered low intensity repetitive magnetic stimulation (LI-rMS) at 10 mT and at frequencies of 1 Hz and 10 Hz to B50 rat neuroblastoma cells in vitro for 10 minutes. Both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. The effects were frequency-specific, with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites suggested that higher spontaneous activity may have led to an increase in GABA release.
Erdal ME, Yılmaz SG, Gürgül S, Uzun C, Derici D, Erdal N. · 2018
Researchers examined the effects of long-term ELF-MF exposure on miRNAs previously related to brain and human diseases, including miR-26b-5p, miR-9-5p, miR-29a-3p, miR-106b-5p, miR-107, and miR-125a-3p. Male and female Wistar-Albino rats were divided into sham and ELF-MF exposed groups, and after sacrifice, blood samples and brain tissues were collected. All miRNA expression levels in young female rats showed a significant decrease in blood compared to brain samples, though fewer miRNAs showed a similar significant decrease in blood.
Bobkova NV et al. · 2018
Researchers studied the effects of a weak combined magnetic field on spatial memory and brain amyloid-β in two mouse models of Alzheimer's disease. Exposure to the magnetic fields for 4 hours per day for 10 days decreased amyloid-β levels in the brains of OBE mice and reduced the number of amyloid-β plaques in the cortex and hippocampus of transgenic mice. Memory improvement was seen in transgenic mice only, not in OBE animals.
Akbarnejad Z et al. · 2018
Researchers injected amyloid-β 1-42 into the lateral ventricle of rats to establish an Alzheimer's disease model, then exposed some groups to a 50 Hz, 10 mT extremely low frequency magnetic field for 14 consecutive days. AD rats showed significant impairment in learning and memory compared to control rats, and spatial memory was assessed using the Morris water maze. The researchers found that the magnetic field improved learning and memory impairments and suggested it also disrupted the processes of AD rat model formation.
Yakymenko I et al. · 2018
Researchers used developing quail embryos to assess the oxidative and mutagenic effects of GSM 1800 MHz microwave radiation from a commercial smartphone. The embryos were exposed in ovo to 0.32 µW/cm2, discontinuously, for 5 days before and 14 days through the incubation period. Exposure resulted in a 2-fold increase in superoxide generation rate, an 85% increase in nitrogen oxide generation rate, and up to twice increased levels of 8-oxo-dG in cells of 1-day old chicks from the exposed embryos. The exposure also resulted in a significant, almost twice, increase of embryo mortality.
Li R et al. · 2018
Mouse spermatocyte-derived cells (GC-2) were exposed to radiofrequency electromagnetic fields at 4 W/kg for 24 hours. The exposure induced both autophagy and DNA damage in GC-2 cells through the generation of reactive oxygen species. When autophagy was blocked by knocking down AMPKα, DNA damage increased, while overexpressing AMPKα promoted autophagy and reduced DNA damage.
Houston BJ, Nixon B, King BV, Aitken RJ, De Iuliis GN. · 2018
Researchers exposed cultured mouse spermatogonial GC1 and spermatocyte GC2 cell lines, as well as cauda epididymal spermatozoa, to continuous wave RF-EMR at 1.8 GHz at 0.15 and 1.5 W/kg. A 4-hour exposure increased mitochondrial reactive oxygen species in GC1 cells (7 vs. 18%; p < 0.001) and GC2 cells (11.5 vs. 16%; p < 0.01), with Complex III of the electron transport chain identified as the potential source. Exposure at 0.15 W/kg for 3 hours induced significant DNA fragmentation in spermatozoa (p < 0.05), though this was no longer significant after 4 hours. After 4 hours of exposure, spermatozoa showed significant oxidative DNA damage in the form of 8-hydroxy-2′-deoxyguanosine (p < 0.05) and a decline in sperm motility (p < 0.05).
Zhang J, Ding C, Meng X, Shang P · 2018
Researchers studied whether nitric oxide modulates the responses of osteoclast formation to static magnetic fields. Field strengths of 500 nT (hypomagnetic field), 0.2 T (moderate static magnetic field), and 16 T (high static magnetic field) were utilized as treatment. At 16 T, osteoclast formation was markedly decreased and nitric oxide synthase activity was enhanced, producing a high level of NO. At 500 nT and 0.2 T, osteoclast formation increased alongside decreased NOS activity and NO production.
Sun Y, Shi Z, Wang Y, Tang C, Liao Y, Yang C, Cai P · 2018
Researchers exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency electromagnetic fields from the egg stage until the fourth larva stage. Reactive oxygen species levels were significantly elevated and total antioxidant capacity was significantly depressed in the exposed worms. The TCA cycle enzyme fumarase showed decreased expression, while arachidonic acid, prostaglandin E2, and prostaglandin E2 synthase all showed increased levels in exposed worms.
Sun L, Chen L, Bai L, Xia Y, Yang X, Jiang W, Sun W. · 2018
Researchers exposed human amnion epithelial (FL) cells to a 50-Hz magnetic field and investigated the role of reactive oxygen species (ROS) in magnetic-field-induced clustering of epidermal growth factor receptor (EGFR). When cells were pretreated with ROS scavengers NAC or PDTC before exposure, both the ROS-related effects and the EGFR clustering were inhibited. The researchers concluded that ROS mediates magnetic-field-induced EGFR clustering via ASMase activation.
Shokrollahi S, Ghanati F, Sajedi RH, Sharifi M · 2018
Researchers exposed soybean plants to static magnetic fields of 0, 20, and 30 mT for 5 days at 5 hours each day, examining ferrous content and the gene expression and activity of ferritin and catalase. At 20 mT, gene expression of the iron transporter, ferrous content, hydrogen peroxide content, and ferritin and catalase gene expression, content, and activity all decreased. Opposite responses were observed under 30 mT treatments. The researchers also evaluated purified ferritin and apoferritin from horse spleen and catalase from bovine liver under the static magnetic field, finding that tertiary structure, size, and activity were altered, while secondary structure was not influenced.
Seif F, Bayatiani MR, Ansarihadipour H, Habibi G, Sadelaji S · 2018
Researchers divided adult male rats into three groups: a control group, a group exposed to extremely low-frequency magnetic fields (ELFMF), and a group given Myrtus communis extract before ELFMF exposure. The magnetic field was set at 0.7 mT and 50 Hz, applied for two hours a day for one month. ELFMF exposure decreased plasma antioxidant power and significantly increased markers of oxidative damage, including protein carbonyl, methemoglobin, and hemichrome concentrations. Administration of M. communis extract increased antioxidant power and decreased plasma protein carbonyl, methemoglobin, and hemichrome concentrations.
Mohammadi F, Ghanati F, Sharifi M, Chashmi NA · 2018
Exposure to the static magnetic field delayed the G1 to S transition in the cell cycle, accompanied by a decrease in cyclin-dependent kinase A and D-type cyclin. The static magnetic field also increased levels of nitric oxide, hydrogen peroxide, and salicylic acid compared to the control group.
Medina-Fernandez FJ et al. · 2018
Researchers studied the effects of transcranial magnetic stimulation (TMS), natalizumab, dimethyl fumarate, and dexamethasone on Dark Agouti rats given a single dose of myelin oligodendrocyte glycoprotein (MOG). MOG triggered a significant increase in clinical score and in levels of lipid peroxides and carbonylated proteins, but reduced the GSH/GSSG ratio in brain, spinal cord and blood. Both TMS and the clinical treatments decreased the changes caused by MOG, with TMS doing so more significantly.
Maliszewska J et al. · 2018
Researchers studied whether a 50 Hz magnetic field affects how American cockroaches respond to noxious heat and alters oxidative stress markers. The exposure was 50 Hz at 7 mT, and the researchers measured how long cockroaches took to escape from high ambient temperature. Escape latency significantly increased after 24 hours, 72 hours, and 7 days of EMF exposure. Malondialdehyde levels increased significantly after 24-hour exposure and remained elevated up to 7 days, while glutathione levels significantly declined after 7 days of exposure.
Mahmoudinasab H, Saadat M. · 2018
Researchers measured the effects of morphine and EMF, in combination with β-lapachone, on cell growth inhibition and expression of antioxidant genes in SH-SY5Y cells. The study used 0.50 mT intensity of 50 Hz EMF under two exposure conditions: "15 min field-on/15 min field-off" and "30 min field-on continuously." Under the "15 min field-on/15 min field-off" condition, NQO1 mRNA level decreased while NQO2 expression increased. Multiple linear regression analysis indicated that NQO1 mRNA levels were associated positively with β-lapachone and negatively with EMF.
Mahmoudinasab H, Saadat M. · 2018
Researchers tested four treatment combinations on MCF-7 and SH-SY5Y cells: cisplatin alone, cisplatin with morin, cisplatin with electromagnetic field, and cisplatin combined with both morin and electromagnetic field. The electromagnetic field was applied at 50 Hz and 0.50 mT using a cycle of 15 minutes field-on followed by 15 minutes field-off. The IC50 value of cisplatin in the cisplatin-plus-morin-plus-EMF treatment was significantly higher than cisplatin alone and the cisplatin-plus-morin treatment in both cell lines. In SH-SY5Y cells, most of the antioxidant genes showed upregulation with the cisplatin-plus-EMF and cisplatin-plus-morin-plus-EMF treatments, while antioxidant gene expression patterns were similar across the four treatments in MCF-7 cells.
