Ozorak A et al. · 2013
Kidney and testis samples were collected from decapitated rats during the fourth, fifth, and sixth weeks. In the fourth week, lipid peroxidation in kidney and testis and copper, zinc, GSH, GSH-Px, and TAS in kidney decreased, while iron in kidney and vitamin A and vitamin E in testis increased in exposed groups. In the fifth week, iron, vitamin A, and β-carotene in kidney increased, while GSH and TAS decreased in exposed groups. In the sixth week, iron concentrations in kidney and lipid peroxidation in kidney and testis increased, while copper, TAS, and GSH concentrations decreased in exposed groups.
Jelodar G, Akbari A, Nazifi S. · 2013
Researchers exposed adult male Sprague-Dawley rats to 900 MHz radiofrequency waves daily for 45 days and measured antioxidant enzymes and malondialdehyde in eye tissue. Radiofrequency wave exposure decreased glutathione peroxidase, superoxide dismutase, and catalase activity and increased malondialdehyde compared with controls. Vitamin C supplementation at 200 mg/kg per day improved antioxidant enzyme activity and reduced malondialdehyde compared with the test group.
Aynali G, Nazıroğlu M, Celik O, Doğan M, Yarıktaş M, Yasan H · 2013
Thirty-two male rats were divided into four groups, including a melatonin-treated group receiving 10 mg/kg/day. Rats in the exposure groups received 2.45 GHz radiation for 60 minutes daily for 28 days. Radiation exposure raised lipid peroxidation levels compared with controls. Melatonin treatment lowered lipid peroxidation and increased glutathione peroxidase activity. Reduced glutathione levels did not differ among groups.
Zhang Y, She F, Li L, Chen C, Xu S, Luo X, Li M, He M, Yu Z. · 2013
Researchers exposed primary cultured cortical neurons from newborn Sprague-Dawley rats to pulsed 2.45 GHz radiofrequency electromagnetic fields for 10 minutes. Exposure significantly decreased cellular viability and increased apoptotic nuclei, CDK5 activity, the p25 to p35 ratio, and tau phosphorylation at Ser404. CDK5 expression did not change significantly after exposure. A CDK5 inhibitor blocked the exposure-induced decrease in cellular viability and tau hyperphosphorylation but did not significantly block apoptosis.
Zhijian C et al. · 2013
The study used protein microarray to examine protein expression in human B lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation at a specific absorption rate of 2.0 W/kg for 24 hours. The analysis identified 27 differentially expressed proteins associated with DNA damage repair, apoptosis, oncogenesis, cell cycle, and proliferation, with a fold change greater than 1.5 and P<0.05. Western blot validation confirmed that RPA32 expression was significantly down-regulated and p73 expression was significantly up-regulated in the exposed group (P<0.05).
Xu S et al. · 2013
Tsybulin O et al. · 2013
Japanese quail embryos were exposed in ovo to GSM 900 MHz radiation for 38 hours or 158 hours with a 48-second on, 12-second off cycle at an average power density of 0.25 μW/cm(2) and a specific absorption rate of 3 μW/kg. The number of differentiated somites was assessed microscopically and DNA damage was assessed by an alkaline comet assay. After 38 hours the number of differentiated somites increased and DNA strand breaks decreased, while after 158 hours the number of differentiated somites decreased and DNA damage increased.
Sun W, Shen X, Lu D, Lu D, Chiang H · 2013
The radiofrequency radiation induced EGF receptor clustering and enhanced phosphorylation on tyrosine-1173 at the higher absorption rates but not at 0.1 W/kg. A 2 μT incoherent magnetic field alone did not significantly affect receptor clustering or phosphorylation. When applied together, the incoherent magnetic field completely inhibited the radiofrequency radiation effects at 0.5, 1.0, and 2.0 W/kg but not at 4.0 W/kg.
Jiang B, Zong C, Zhao H, Ji Y, Tong J, Cao Y · 2013
Adult male ICR mice were pre-exposed to 900 MHz radiofrequency fields for 4 hours per day for 7 days, then given an acute whole-body dose of 3 Gy gamma radiation. The micronucleus assay was used to measure genotoxicity in immature erythrocytes in peripheral blood and bone marrow. Mice exposed to the radiofrequency fields alone showed micronucleus indices similar to unexposed controls, while mice given gamma radiation alone showed a significantly increased micronucleus frequency. Mice pre-exposed to the radiofrequency fields before gamma radiation showed a significant decrease in micronucleus indices compared to those given gamma radiation alone.
Gapeyev AB, Kulagina TP, Aripovsky AV. · 2013
Researchers studied how extremely high-frequency electromagnetic radiation (EHF EMR) affected fatty acid composition in the thymus, liver, blood plasma, muscle tissue, and tumor tissue of mice carrying Ehrlich solid carcinoma. Mice were exposed to EHF EMR at 42.2 GHz, 0.1 mW/cm2, for 20 minutes daily over five consecutive days starting the day after tumor cells were introduced. In tumor-bearing mice, EHF EMR exposure led to recovery of fatty acid composition in thymocytes to the state typical for normal animals, and in tumor tissue it significantly decreased monounsaturated fatty acids and increased polyunsaturated fatty acids.
Atlı Şekeroğlu Z, Akar A, Sekeroğlu V. · 2013
Researchers investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields on bone marrow cells of immature and mature rats. Rats in the treatment groups were exposed to RF-EMF for 2 hours per day for 45 days. Significant differences were observed in chromosome aberrations, micronucleus frequency, mitotic index, and ratio of polychromatic erythrocytes in all treatment and recovery groups. A 15-day recovery period after exposure did not reduce the damage to the same extent as the corresponding control groups.
Tkalec M, Stambuk A, Srut M, Malarić K, Klobučar GI. · 2013
Researchers exposed earthworms (Eisenia fetida) in vivo to 900 MHz radiofrequency electromagnetic fields at field levels of 10, 23, 41 and 120 V m(-1) for 2 hours using a Gigahertz Transversal Electromagnetic (GTEM) cell. All exposure treatments induced significant genotoxic effects in earthworm coelomocytes, as detected by the Comet assay. The results also indicated enhanced catalase and glutathione reductase activity, as well as lipid and protein oxidative damage. Antioxidant responses and the potential for damage to lipids, proteins, and DNA differed depending on field level, field modulation, and duration of exposure.
Burlaka A et al. · 2013
Researchers exposed Japanese quail embryos in ovo to GSM 900 MHz radiofrequency radiation at 0.25 µW/cm2 for 158 to 360 hours on a discontinuous schedule. The exposure produced a significant persistent overproduction of superoxide and nitrogen oxide in embryo cells throughout the analysis period. Exposed embryo cells also showed significantly increased levels of TBARS and 8-oxo-dG alongside significantly decreased superoxide dismutase and catalase activities.
Wang H et al. · 2013
Researchers exposed 100 Wistar rats to a 2.856 GHz pulsed microwave field at average power densities of 0, 5, 10, and 50 mW/cm² for 6 minutes. Rats exposed to 10 or 50 mW/cm² showed significant deficits in spatial learning and memory at 6 hours, 1 day, and 3 days after exposure. At the 5 mW/cm² level, exposed rats showed no difference compared with the sham group. The study suggested that impairment of LTP induction and damages of hippocampal structure, especially changes of synapses, might contribute to cognitive impairment after microwave exposure.
Sharma A, Sisodia R, Bhatnagar D, Saxena VK · 2013
Researchers exposed Swiss albino mice to 10 GHz microwaves at a power density of 0.25 mW/cm2 for 2 hours per day for 30 days, then tested spatial memory using the Morris water maze test. Exposed mice showed statistically significant higher mean latency to reach the target quadrant compared to sham-exposed mice. A decrease in protein levels was also estimated in the whole brain of exposed mice compared to sham-exposed mice.
Ros-Llor I et al. · 2012
Researchers conducted a cohort study of 50 Caucasian mobile phone users, collecting two cheek mucosa cell samples from each participant. No statistically significant changes were recorded for age, gender, body mass index, or smoking status, and comparisons by phone side and exposure duration showed no significant differences. The authors reported no genotoxic effects from RF exposure for any study parameter.
Marjanović AM, Pavičić I, Trošić I · 2012
Radiation from man-made sources has exceeded natural radiation due to rapid technological progress. There is general concern about appliances using radiofrequency and microwave radiation, especially mobile communication systems. The authors identify reactive oxygen species generation as a plausible mechanism for radiofrequency and microwave radiation effects. Excessive reactive oxygen species production can overload antioxidant defenses and lead to cellular damage with potential for disease development. Further research is needed to understand the exact mechanism and consequences.
Laudisi F et al. · 2012
Researchers exposed pregnant mice to WiFi signals at 2450 MHz with a specific absorption rate of 4 W/kg for two hours daily during gestation. Thymocyte count, phenotype, and proliferation were unchanged in exposed offspring. These null effects were consistent in both sexes at 5 and 26 weeks of age.
Bourthoumieu S et al. · 2012
Researchers exposed human amniotic cells to GSM-900 radiofrequency fields for 24 hours at SARs of 0.25, 1, 2, and 4 W/kg. They measured p53 protein expression and phosphorylation at serine 15 and 37 using Western blot after three independent exposures from three donors. No significant changes in p53 expression or activation were found at any of the tested SARs up to 4 W/kg in human embryonic cells. Bleomycin-exposed cells served as a positive control.
Hong MN et al. · 2012
Researchers exposed human MCF10A mammary epithelial cells to single frequencies of 837 MHz or 1950 MHz, or both frequencies together, at a SAR of 4 W/kg for 2 hours while maintaining isothermal temperature. They measured intracellular ROS, SOD enzyme activity, and GSH/GSSG ratio and found no statistically significant alterations from either single or multiple RF radiation exposures. Ionizing radiation used as a positive control showed evident changes in all measured endpoints. The results indicate that single or multiple RF radiation exposure did not elicit oxidative stress in MCF10A cells under these conditions.
Ozlem Nisbet H, Nisbet C, Akar A, Cevik M, Karayigit MO · 2012
Nazıroğlu M, Ciğ B, Doğan S, Uğuz AC, Dilek S, Faouzi D. · 2012
Researchers divided twelve cell cultures into control and irradiated groups of six each, then further split them into four exposure durations of 1, 2, 12, and 24 hours. Exposed cells showed higher lipid peroxidation, cytosolic free Ca²⁺, and cell numbers than controls. The rise in cytosolic free Ca²⁺ was radiation time-dependent and highest at 24 hours. Reduced glutathione, glutathione peroxidase, vitamin C, and cell viability did not change in any group. 2-aminoethyl diphenylborinate inhibited Ca²⁺ influx by blocking transient receptor potential melastatin 2 channels.
Ingole IV, Ghosh SK. · 2012
Researchers exposed chick embryos to radiofrequency radiation emitted by a cell phone by varying the duration of exposure to create different doses. Light microscopic examination revealed that developing dorsal root ganglion neurons suffered damage that was dose dependent. The damage persisted even when exposure-free periods were given between exposures.
Fattahi-Asl J et al. · 2012
Fifty human serum wells from 25 normal healthy donors were labeled with ruthenium for an immunoassay and placed into two batches. The first batch was exposed to 900 MHz radiation from a Nokia 1202 cell phone in speech mode for 30 minutes. Ferritin levels in the exposed batch averaged 84.94 ± 1.04 μg/L versus 87.25 ± 0.83 μg/L in the unexposed batch, a significant decrease (P = 0.029).
Ceyhan AM et al. · 2012
Thirty-two male Wistar albino rats were divided into control, sham, electromagnetic radiation, and radiation plus β-glucan groups. Rats in the radiation groups were exposed to a 2.45-GHz field for 60 minutes daily over four weeks, and β-glucan was given at 50 mg/kg/day by gavage before each exposure. Radiation increased malondialdehyde and catalase activity and decreased superoxide dismutase and glutathione peroxidase activities, while β-glucan significantly reversed the malondialdehyde rise and the superoxide dismutase drop, with non-significant effects on catalase and glutathione peroxidase.
