Zeni O et al. · 2012
When lymphocytes from six donors were pre-exposed to RF at 0.3W/kg SAR and then treated with MMC, these cells showed a significant reduction in the frequency of MN compared with cells treated with MMC alone, indicating adaptive response. An earlier study found that lymphocytes stimulated for 24h, exposed for 20h to a 900MHz RF GSM signal at 1.25W/kg SAR and then treated with 100ng/ml MMC also exhibited adaptive response. In the current study, cells from nine healthy human volunteers were stimulated for 24h with phytohaemagglutinin, exposed for 20h to a 1950MHz RF UMTS signal at SARs of 1.25, 0.6, 0.3, and 0.15W/kg, then treated at 48h with 100ng/ml mitomycin C, and micronuclei frequency was recorded at 72h using the cytokinesis-block method.
Yang L, Hao D, Wang M, Zeng Y, Wu S, Zeng Y. · 2012
Researchers exposed NIH/3T3 cells to 916 MHz continuous microwave radiation for 2 h per day at power densities of 10, 50, and 90 w/m(2). After 5 to 8 weeks, the exposed cells showed changes in morphology and proliferation and formed colonies in soft agar after another 3 to 4 weeks. When injected into SCID mice, the exposed cells produced lumps on the animals' backs after more than 4 weeks.
Sun W, Shen X, Lu D, Fu Y, Lu D, Chiang H · 2012
Researchers investigated the effects of 1.8-GHz RFR at different intensities on EGF receptor clustering and phosphorylation in human amniotic (FL) cells. Receptor clustering was analyzed by immunofluorescence with confocal microscopy, and phosphorylation was measured by western blot. Exposure at SAR 0.5, 1.0, 2.0, and 4.0 W/kg significantly induced clustering and phosphorylation, while SAR 0.1 W/kg had no significant effect. The authors concluded that membrane receptors may be a primary target of RFR, with a dose-rate threshold between 0.1 and 0.5 W/kg and a sigmoid dependence on intensity.
Solomentsev GY, English NJ, Mooney DA · 2012
Researchers performed non-equilibrium molecular dynamics simulations of a solvated 21-residue polyalanine peptide at 300 K and 1 bar with external electromagnetic fields at 2.45 GHz and electric field intensities of 0.01–0.05 V/Å. The peptide naturally tends to form stable secondary structure elements stabilized by tertiary interactions. External fields disrupted this behavior through localized dipolar alignment that enhanced hydrogen bond perturbations, causing more frequent transitions between shorter-lived conformational states. The study reported 16 independent 40-nanosecond trajectories starting from both extended and fully folded states.
Misa Agustiño MJ et al. · 2012
Fifty-four adult female Sprague-Dawley rats were individually exposed to 2.45 GHz radiation for 30 minutes in a GTEM cell at non-thermal SAR levels. Ninety minutes after exposure HSP-90 and HSP-70 decreased significantly at SAR values of 0.046±1.10 W/Kg and 0.104±5.10−3 W/Kg. Twenty-four hours after exposure HSP-90 had partially recovered and HSP-70 had recovered completely. There were few indications of lesions in the glandular structure and signs of apoptosis were negative in all radiated animals.
Kumar S, Behari J, Sisodia R. · 2012
Researchers exposed 70-day-old male albino rats to 10-GHz EMF for 2 hours a day over 45 days. Creatine kinase phosphorylation increased in sperm after exposure. Melatonin and MDA levels decreased in exposed rats. The authors concluded that microwave exposure could adversely affect male fertility.
Kesari KK, Kumar S, Behari J. · 2012
Researchers exposed Wistar rats to 2.45 GHz frequency microwave radiation for 2 h a day for 45 days, with a whole body specific absorption rate estimated to be 0.14 W/kg. Pineal melatonin levels were significantly lower in the exposed group compared with the sham-exposed group. Creatine kinase, caspase 3, and calcium ion concentration were all significantly higher in the whole brain of the exposed animals.
Jin Z, Zong C, Jiang B, Zhou Z, Tong J, Cao Y. · 2012
Researchers pre-exposed human promyelocytic leukemia HL-60 cells to 900 MHz radiofrequency fields at 12 µW/cm2 power density for 1 hour/day for 3 days, then treated them with doxorubicin at 0.125 mg/L. Radiofrequency exposure alone showed no significant differences from unexposed or sham-exposed control cells. Compared with doxorubicin treatment alone, cells that received radiofrequency pre-exposure before doxorubicin showed increased cell proliferation, decreased apoptosis, increased mitochondrial membrane potential, decreased intracellular calcium, and increased Ca2+-Mg2+-ATPase activity.
Hekmat A, Saboury AA, Moosavi-Movahedi AA. · 2012
Researchers exposed calf thymus DNA to mobile phone radiofrequency fields at 940 MHz, 15 V/m, and a SAR of 40 mW/kg, then examined the DNA immediately after exposure and again two hours after a 45-minute exposure using several laboratory instruments. UV-vis and circular dichroism experiments showed that the EMFs caused disturbance in the DNA structure. Exposed DNA samples were relatively thermally unstable compared to unexposed DNA, and showed more fluorescence emission, which the researchers suggested may have occurred due to expansion of the exposed DNA structure. The structure of the DNA immediately after exposure was not significantly different from the DNA sample two hours after the 45-minute exposure, suggesting the changes were irreversible.
Akar A et al. · 2012
Researchers exposed adult male Wistar rats to 2.45 GHz microwave radiation at a SAR of 0.25 W/kg for 2 hours each day for 21 days and then measured corneal thickness using histological and stereological methods. Overall corneal thickness was 278.9 ± 54.5 μm in the control group and 272.4 ± 85.6 μm in the study group, with no statistically significant difference between groups. Anterior corneal epithelium thickness was 28.1 ± 4.9 μm in the control group and 31.7 ± 5.5 μm in the exposed group, a statistically significant difference. The researchers concluded that exposure to microwave radiation might cause alterations in the rat cornea.
Jiang B, Nie J, Zhou Z, Zhang J, Tong J, Cao Y. · 2012
Researchers pre-exposed mice to 900 MHz radiofrequency fields at 120 µW/cm2 for 4 hours per day for 1, 3, 5, 7, or 14 days, then subjected them to an acute dose of 3 Gy of gamma-radiation. DNA damage in peripheral blood leukocytes was measured using the alkaline comet assay, which detects alkali labile base damage and single strand breaks. Mice pre-exposed to radiofrequency fields for just 1 day showed damage similar to those exposed to gamma-radiation alone, but pre-exposure for 3 or more days led to progressively decreased damage.
Lu YS, Huang BT, Huang YX. · 2012
Researchers studied reactive oxygen species (ROS) formation and apoptosis in human peripheral blood mononuclear cells (PBMC) exposed to 900 MHz radiofrequency electromagnetic fields at a specific absorption rate of ~0.4 W/kg. ROS was found to play an important role in apoptosis when exposure lasted longer than two hours, with apoptosis induced through the mitochondrial pathway and mediated by activating ROS and caspase-3, and decreasing the mitochondrial potential. The imbalance between antioxidant defenses and ROS formation was associated with about 37% human PBMC death in eight hours.
Güler G et al. · 2012
Researchers exposed infant rabbits to 1800 MHz GSM-like RF radiation and measured lipid peroxidation and DNA damage in their livers. Lipid peroxidation levels in liver tissue increased in both female and male infant rabbits under RF radiation exposure. Liver 8-OHdG levels, a marker of DNA damage, increased in exposed female rabbits but showed no change in exposed male rabbits. The researchers concluded that GSM-like RF radiation may induce biochemical changes by increasing free radical attacks to structural biomolecules in the rabbit model.
Pilla AA · 2012
Researchers tested how a non-thermal pulse-modulated RF signal affected nitric oxide release in dopaminergic MN9D neuronal cells challenged with lipopolysaccharides and in human fibroblast cultures challenged with low serum. In dopaminergic MN9D cultures, the signal produced an immediate nearly three-fold increase in nitric oxide, measured in real-time using a nitric oxide selective membrane electrode. In human fibroblast cultures challenged with low serum and exposed for 15 minutes to the same signal, nitric oxide increased nearly two-fold, and this increase could be blocked by the calmodulin antagonist W-7.
Yang XS, He GL, Hao YT, Xiao Y, Chen CH, Zhang GB, Yu ZP. · 2012
Researchers exposed adult male Sprague-Dawley rats to a 2.45 GHz electromagnetic field at a specific absorption rate of 6 W/kg, or to sham conditions, then examined gene and protein expression in the hippocampus. Of 2048 candidate genes examined, 23 were upregulated and 18 were downregulated. Two heat shock proteins, HSP27 and HSP70, stood out because their expression levels were increased in the rat hippocampus. The researchers concluded that their data provide direct evidence that exposure to electromagnetic fields elicits a stress response in the rat hippocampus.
Nazıroğlu M et al. · 2012
Researchers divided rats into four groups: a cage control, a sham control, a group exposed to 2.45 GHz electromagnetic radiation, and a group exposed to the same radiation while also receiving melatonin. The radiation-exposed groups received that exposure for 60 minutes per day for 30 days, after which brain cortex and dorsal root ganglion samples and EEG recordings were collected. Lipid peroxidation, cell viability, and cytosolic calcium values in dorsal root ganglion neurons were higher in the radiation-only group than in the control groups. The melatonin-plus-radiation group showed lower EEG spike numbers and higher brain cortex vitamin E concentration compared with the radiation-only group.
Maskey D, Kim HJ, Kim HG, Kim MJ. · 2012
Researchers exposed mice to 835 MHz radiofrequency at SAR values of 1.6 W/kg and 4.0 W/kg for one month to study effects in the hippocampus. Compared with a sham control group, both exposed groups showed decreased calbindin D28-k and calretinin, loss of immunoreactive cells, and increased GFAP with hypertrophic cytoplasmic processes. The group exposed at 4.0 W/kg showed a more prominent decrease in calbindin and calretinin, and more prominent GFAP immunoreactivity, than the group exposed at 1.6 W/kg.
Lu Y et al. · 2012
Researchers exposed Wistar rats to 2.45 GHz pulsed microwave irradiation at a power density of 1 mW/cm2 for 3 hours per day for up to 30 days. Microwave exposure induced spatial learning and memory impairments in rats, and reduced hippocampal glucose uptake in the absence or presence of insulin. Blood glucose and insulin levels were not affected, but spatial memory deficits were reversed by systemic glucose treatment.
Cogulu O. · 2012
Researchers exposed mouse brain cell cultures to radiofrequency waves to evaluate genotoxic effects. The cultures were exposed to 10.715 GHz signals at a specific absorption rate of 0.725 W/kg for 6 hours over 3 days at 25°C. The study measured changes in the micronucleus assay and in the expression of 11 proapoptotic and antiapoptotic genes. The micronuclei rate increased 11-fold and STAT3 expression decreased 7-fold in the exposed cell cultures.
Chen G, Lu D, Chiang H, Leszczynski D, Xu Z. · 2012
Researchers used the yeast Saccharomyces cerevisiae to study how exposure to extremely low frequency magnetic fields and radiofrequency EMF affects gene expression. Yeast cells were exposed for 6 hours to either 0.4 mT 50 Hz ELF-MF or 1800 MHz RF-EMF at a specific absorption rate of 4.7 W/kg. The study found no confirmed gene expression changes from ELF-MF exposure, and only the expressions of SMC3 and AQY2 were confirmed as responsive to RF-EMF.
Bourthoumieu S et al. · 2011
Researchers investigated the potential induction of genomic instability by GSM-900 MHz radiofrequency radiation in human amniotic cells exposed for 24 h to average-specific absorption rates (SAR) of 0.25, 1, 2 and 4 W/kg. The rate of aneuploidy of chromosomes 11 and 17 was determined by interphase FISH immediately after independent exposure of three different donors for 24 h. No significant change in the rate of aneuploidy of chromosomes 11 and 17 was found at average SAR up to 4 W/kg.
Sakurai T et al. · 2011
The study used DNA microarray to examine gene expression in SVGp12 human glial cells after exposure to 2.45 GHz continuous-wave RF fields at SARs of 1, 5, and 10 W/kg for 1, 4, and 24 hours. Microarray analysis identified 23 assigned and 5 non-assigned gene spots as potentially altered. Follow-up RT-PCR analysis of Twenty-two genes showed no significant alterations in gene expression. The authors concluded that under their experimental conditions they found no evidence that RF field exposure affected gene expression in SVGp12 cells.
Lukac N et al. · 2011
Researchers exposed bovine semen to 1800 MHz GSM-like RF-EMW in vitro and measured motility at 0, 30, 120, and 420 minutes using a computer-assisted semen analyzer. The percentage of motile and progressively motile sperm decreased significantly after 120 and 420 minutes of exposure. Average path distance decreased after 30 and 420 minutes, while path velocity initially increased at 30 minutes but decreased at 420 minutes. Amplitude of lateral head displacement also decreased significantly after 420 minutes.
Jorge-Mora T et al. · 2011
Researchers investigated the effects of 2.45 GHz microwave radiation on the paraventricular nucleus of rat hypothalamus using c-Fos protein expression as a marker of cellular activation. Rats were exposed once or repeatedly (ten times in two weeks) to 2.45 GHz radiation at power intensities of 3 and 12 W in a GTEM cell, with SAR determined by Finite Difference Time Domain calculation. High SAR increased c-Fos expression at 90 minutes and 24 hours post-exposure, low SAR increased c-Fos counts at 24 hours, and repeated 3 W exposure increased PVN activation by more than 100% compared to acute exposure and non-radiated controls.
Garaj-Vrhovac V et al. · 2011
The study examined workers occupationally exposed to pulsed microwave radiation from marine radars. Comet assay parameters (mean tail intensity 0.67 vs 1.22, moment 0.08 vs 0.16) and micronucleus test parameters showed statistically significant differences between exposed and unexposed groups. Glutathione concentration was significantly lower in exposed workers (1.24 vs 0.53) while malondialdehyde concentration was significantly higher (1.74 vs 3.17), indicating oxidative stress. Electromagnetic field strength was measured at marine radar frequencies of 3 GHz, 5.5 GHz, and 9.4 GHz with specific absorption rate values determined.
