Gok DK et al. · 2016
Researchers divided Wistar albino female rats into four groups: a control group, a prenatal-exposure group, a postnatal-exposure group, and a combined prenatal-plus-postnatal-exposure group. Pregnant rats in the prenatal and combined groups were exposed to a 50 Hz electric field at 12 kV/m for 1 hour per day during pregnancy, while postnatal animals were exposed for 68 days after birth. The latencies of visual evoked potential components were significantly prolonged in all exposed groups compared with controls. Retina TBARS levels, a marker of lipid peroxidation, were significantly elevated in all experimental groups compared with controls.
Feng B, Ye C, Qiu L, Chen L, Fu Y, Sun W · 2016
Human amniotic FL cells were exposed to a 50-Hz magnetic field for different durations or intensities, then incubated with staurosporine for 4 hours. Magnetic field exposure alone had no significant effect on early apoptosis or cell viability. When cells were also treated with staurosporine, magnetic field exposure had a protective effect against early apoptosis, and this effect could be abolished by MPT inhibitors. The anti-apoptotic effect was completely eliminated when Akt was inhibited, suggesting that Akt activation was necessary for the effect.
Feng B, Dai A, Chen L, Qiu L, Fu Y, Sun W. · 2016
Researchers exposed human amniotic epithelial (FL) cells to a 50-Hz magnetic field to investigate the molecular mechanisms behind magnetic field-induced epidermal growth factor receptor clustering. Exposing FL cells to the magnetic field at intensities higher than 0.2 mT for 15 minutes enhanced total reactive oxygen species production. Blocking the NADPH oxidase enzyme with the inhibitor DPI suppressed magnetic field-induced cytoplasmic superoxide production and subsequent EGFR clustering, while mitochondrial superoxide production was not affected.
Feng B, Qiu L, Ye C, Chen L, Fu Y, Sun W. · 2016
Researchers exposed human amniotic epithelial cells to a 50-Hz magnetic field at 0.4 mT and examined mitochondrial permeability, mitochondrial membrane potential, and cytochrome c release. Exposure to the magnetic field at 0.4 mT for 60 min transiently induced mitochondrial permeability transition and Cyt-c release, although there was no significant effect on mitochondrial membrane potential. Cells exposed to the magnetic field showed increased intracellular reactive oxidative species levels and GSK-3β dephosphorylation, and the induced mitochondrial permeability transition was attenuated by a ROS scavenger or GSK-3β inhibitor.
Falone S et al. · 2016
Researchers exposed SK-N-BE(2) drug-resistant human neuroblastoma cells to a pulsed electromagnetic field and then challenged the cells with hydrogen peroxide to measure oxidative stress responses. The PEMF increased MnSOD-based antioxidant protection and reduced ROS production in response to a pro-oxidant challenge. The researchers suggested their work might lay foundation for the development of non-invasive PEMF-based approaches aimed at elevating endogenous antioxidant properties in cellular or tissue models.
Duong CN, Kim JY · 2016
Researchers studied whether EMF exposure could protect a human microglial cell line, HMO6, from cell death caused by oxygen-glucose deprivation (OGD), a model of ischemic injury. HMO6 cells were cultured for 4 hours under OGD with or without exposure to EMF at different combinations of frequencies and intensities, and cell survival, intracellular calcium, and ROS levels were measured. Among the combinations tested, 50 Hz/1 mT EMF was the most potent at reducing OGD-induced cell death and lowering intracellular calcium and ROS levels. A significant but less potent protective effect was also found at 10 Hz/1 mT, while no protective effect was found at other combinations.
Benassi B et al. · 2016
Researchers exposed SH-SY5Y cells to an extremely low frequency magnetic field set up to reproduce environmental prolonged exposure to ELF-MF (50 Hz, 1 mT) and then treated the cells with the pro-Parkinson's toxin MPP+. ELF-MF alone did not affect cell survival, shape, or morphology, but it did significantly impair redox homeostasis and thiol content, triggering an increase in protein carbonylation. When MPP+ was added to ELF-MF-exposed cells, toxicity was highly enhanced, with significant increases in ROS levels, oxidative damage, and caspase-dependent apoptosis. Pre-incubation with the thiol antioxidants N-acetyl-L-cysteine and GSH ethyl-ester significantly reduced oxidative damage and protected cells from death caused by the combined ELF-MF/MPP+ treatment.
Ansari AM et al. · 2016
Researchers exposed male adult NMRI mice to an extremely low frequency magnetic field at 0.5 mT and 50 Hz, either as a single 2-hour session or for 2 hours a day over 2 weeks. Depressive-like behavior was measured using the Forced Swimming Test, which records immobility time. Long-term exposure significantly reduced immobility time, while short-term exposure did not alter it.
Akpınar D et al. · 2016
Researchers exposed rats to an electric field at 12 kV/m for 1 hour per day, dividing them into control, prenatal, postnatal, and prenatal-plus-postnatal groups. The mismatch negativity amplitude was decreased in all exposed groups compared with the control group. Levels of 4-HNE, a marker of lipid peroxidation, were significantly increased in the postnatal group compared with the control group, while protein carbonyl levels were significantly decreased in the prenatal-plus-postnatal group compared with the control, prenatal, and postnatal groups.
Wang LF et al. · 2016
Researchers exposed Wistar rats to microwave radiation at 30 mW/cm2 for 5 minutes per day, 5 days per week, over a period of 2 months. Screening of the GRIN2B promoter region revealed a stable C-to-T variant at nucleotide position −217 that was not induced by microwave exposure. Following microwave exposure, NR2B protein expression decreased, while the Glu contents in the hippocampus and CSF increased, and memory impairment was observed in the TT genotype but not the CC and CT genotypes. In PC12 cells, the effects of the T allele were more pronounced than those of the C allele on transcription factor binding ability, transcriptional activity, NR2B mRNA, and protein expression.
Sırav B, Seyhan N · 2016
Researchers used young adult male and female Wistar albino rats to investigate whether pulse modulated 900 MHz and 1800 MHz radio-frequency radiation affects the permeability of the blood-brain barrier. Permeability was measured by quantifying Evans blue dye that passed into the brain after intravenous injection. In both male radio-frequency radiation exposed groups, blood-brain barrier permeability was found to be increased compared with controls, with 1800 MHz found more effective in males. For female rats, 900 MHz exposure was associated with increased permeability, but no statistical difference was found between the 1800 MHz exposed and control groups.
Nirwane A, Sridhar V, Majumdar A · 2016
Hidisoglu E et al. · 2016
Researchers exposed rats to 2100-MHz electromagnetic fields for 2 hours per day for either 1 or 10 weeks, then recorded visual evoked potentials and measured markers of oxidative stress in the brain. After 1 week of exposure, visual response latencies were shortened and several antioxidant markers were increased. After 1 or 10 weeks, the effects on visual response latencies differed: latencies were shortened in the 1-week group but prolonged in the 10-week group. The researchers concluded that the effects of EMF on visual evoked potentials depend on exposure duration.
Erdem Koç G et al. · 2016
Researchers established seven groups of pregnant rats and exposed some to 900 MHz EMF for 60 minutes per day during the gestation period. After birth, male rat pups were selected and the total number of pyramidal neurons in the hippocampal region was counted using the optical fractionator technique. The total number of pyramidal cells in the EMF group was significantly lower than in the control, sham, EMF plus melatonin, and EMF plus omega-3 groups. No difference was observed between any groups in terms of rats' body or brain weights.
Deshmukh PS et al. · 2016
Researchers exposed male Fischer rats to microwave radiation for 90 days at 900, 1800, and 2450 MHz to investigate effects on cognitive function, heat shock protein 70 (HSP70) levels, and DNA damage in the brain. Cognitive function was tested using the elevated plus maze and Morris water maze, HSP70 levels were measured by ELISA, and DNA damage was assessed using alkaline comet assay. The results suggest that low-level microwave exposure at these frequencies may lead to hazardous effects on the brain.
(E) Barthélémy A et al. · 2016
Researchers exposed male Sprague Dawley rats for 15 minutes at 0, 1.5, or 6 W/kg, or for 45 minutes at 6 W/kg, to radiofrequency electromagnetic fields. Memory, emotionality, and locomotion were tested using fear conditioning, the elevated plus maze, and the open field. Long-term memory was reduced and cytosolic GFAP was increased in the hippocampus and in the olfactory bulb at 6 W/kg. No MBP or Aβ1–40 expression modification was shown. The study suggests that RF EMF-induced astrogliosis had functional consequences on memory but did not demonstrate that it was secondary to neuronal damage.
Yoon S, Choi JW, Lee E, Ahn H, Kim HS, Choi HD, Kim N. · 2015
Korean researchers studied 285 brain tumor patients and 285 healthy controls to investigate whether mobile phone use increases the risk of gliomas, a serious type of brain cancer. The study found no significant overall increase in brain tumor risk from mobile phone use, though there was a non-significant trend toward higher risk when people used phones on the same side of their head where the tumor developed. The researchers concluded their findings don't support the idea that mobile phones cause brain tumors, but suggested more research is needed for long-term users.
Söderqvist F, Carlberg M, Hardell L · 2015
Swedish researchers tested whether cell phone radiation affects the blood-brain barrier (the brain's protective shield) by measuring specific proteins in blood samples from 24 volunteers before and after exposure to phone-like signals. The study found no significant differences in these barrier-protecting proteins between real exposure and fake exposure sessions. However, the researchers noted that all participants were regular cell phone users, which may have influenced the results.
Shrestha M, Raitanen J, Salminen T, Lahkola A, Auvinen A · 2015
Finnish researchers studied whether mobile phone use increases the risk of pituitary tumors by comparing 80 tumor patients with 240 healthy controls. They found no increased risk of pituitary tumors from mobile phone use, even after 10 years of use. However, the study had limited data on very long-term users (beyond 10 years), so questions remain about potential risks from decades of use.
Schoeni A, Roser K, Röösli M. · 2015
Researchers studied 439 Swiss adolescents to see how nighttime mobile phone interruptions affect their health and thinking abilities. They found that teens awakened by phones at least once monthly were 86% more likely to experience daytime tiredness and over twice as likely to feel rapidly exhausted. However, the interruptions didn't impair memory or concentration on cognitive tests, suggesting the main impact is on energy levels rather than mental performance.
Malkemper EP et al. · 2015
Researchers tested whether wood mice can sense magnetic fields by observing where they built nests in circular arenas. They found that mice normally oriented their nests north-south using Earth's magnetic field, but when exposed to weak radio frequency fields (0.9-5 MHz), the mice switched to building nests east-west instead. This demonstrates that low-level RF exposure can disrupt an animal's natural magnetic navigation system.
Liu YX et al. · 2015
Researchers exposed human brain tumor cells to 3G mobile phone radiation (1950-MHz) for up to 48 hours at high power levels (SAR of 5 W/kg) to see if the radiation would promote tumor growth or change cell behavior. They found no significant effects on cell growth, gene expression, or tumor formation ability. This suggests that 3G signals at these exposure levels don't act as tumor-promoting agents in already-existing brain cancer cells.
Kottou S et al. · 2015
Greek researchers measured electromagnetic field levels in over 4,500 indoor locations across three regions, focusing on extremely low frequency magnetic fields and radiofrequency electric fields that health agencies consider possibly cancer-causing. They found that while EMF levels varied by location and distance from sources, all measurements remained well below current safety limits set by European regulators. The study provides baseline data on typical indoor EMF exposure levels in Greek homes.
Gurbuz N, Sirav B, Kuzay D, Ozer C, Seyhan N. · 2015
Researchers exposed diabetic rats to cell phone radiation (2100 MHz) to see if it caused genetic damage in bladder cells by looking for micronuclei - small fragments of broken DNA that indicate cellular damage. They found no increase in genetic damage in either healthy or diabetic rats exposed to the radiation compared to unexposed animals. The study suggests that this level of RF radiation may not cause DNA damage in bladder cells, even in animals with diabetes who might be more vulnerable.
Dabouis V et al. · 2015
French researchers tracked 57,000 naval personnel over 26 years to compare death rates between those working with radar systems and those in non-radar roles. They found no increased risk of death from any cause, including cancer, among radar-exposed workers compared to unexposed personnel. This large-scale occupational study suggests that the radiofrequency radiation from naval radar systems did not significantly impact mortality rates during the study period.
