Poulletier de Gannes F et al. · 2011
Researchers exposed three human brain cell lines and primary cortical neurons to the EDGE signal at 1800 MHz. Four exposure conditions were tested: 2 and 10 W/kg for 1 and 24 hours. Reactive oxygen species production was measured by flow cytometry using the DCFH-DA probe. All cells tested responded to rotenone treatment by increasing ROS production. The EDGE signal did not induce oxidative stress under any test condition, including at 10 W/kg.
Lee JW, Kim MS, Kim YJ, Choi YJ, Lee Y, Chung HW. · 2011
Researchers investigated the genotoxic potential of 3 T clinical MRI scans in cultured human lymphocytes by exposing them to electromagnetic fields from a clinical brain protocol for 22, 45, 67, and 89 minutes. They observed a significant increase in single-strand DNA breaks after exposure. Micronuclei and chromosome aberration frequencies increased in a time-dependent manner.
Kumar S, Kesari KK, Behari J. · 2011
Esmekaya MA et al. · 2011
Researchers investigated the mutagenic and morphologic effects of 1.8GHz GSM radiofrequency radiation, alone and combined with Ginkgo biloba (EGb 761) pre-treatment, in human peripheral blood lymphocytes using Sister Chromatid Exchange and electron microscopy. There was a significant increase (p<0.05) in Sister Chromatid Exchange frequency in RF-exposed lymphocytes compared to sham controls. RF radiation also inhibited cell viability in a time-dependent manner, and EGb 761 pre-treatment significantly increased cell viability at 8 and 24 hours compared to RF-exposed groups alone.
Aly H., Ashraf & deris, Safaai & Zaki, Nazar. (2011). · 2011
Researchers investigated whether radiofrequency field exposure could affect the activity of human immune system cells by studying the activity and behavior of human leukocytes upon exposure to radiofrequency fields. Human blood leukocytes were exposed to 1800 MHz RF radiation (SAR of 1.25 W/Kg, Power density of 0.1 mW/cm2) from a mobile phone when placed within a distance of 5 cm from the microscope stage. The maximum speed exceeded that measured at any temperature by approximately 50%, and a significant change was observed in leukocyte movement direction and cell behavior, including shrinking, expanding, and rolling.
Falzone N, Huyser C, Becker P, Leszczynski D, Franken DR. · 2011
Highly motile human spermatozoa were exposed for 1 hour to 900-MHz mobile phone radiation at a specific absorption rate of 2.0 W/kg and examined at various times after exposure. The radiation did not affect the sperm acrosome reaction, but significant reductions were found in sperm head area and acrosome percentage of the head area among exposed sperm compared with unexposed controls. The mean number of zona-bound sperm was 22.8 in exposed samples versus 31.8 in controls, a statistically significant difference (p < 0.05). The researchers concluded that these results could indicate a significant effect of RF-EMF on sperm fertilization potential.
Dode AC et al. · 2011
Researchers examined a spatial correlation between cellular base station clusters and deaths by neoplasia in Belo Horizonte, Brazil, from 1996 to 2006. The study used a descriptive ecological-epidemiological approach with georeferencing, drawing on death records, base station registrations, and census data. Between 1996 and 2006, 7191 deaths by neoplasia occurred, and within 500 m of a base station the mortality rate was 34.76 per 10,000 inhabitants, with a decrease in deaths observed outside that area.
Cao Y, Xu Q, Jin ZD, Zhou Z, Nie JH, Tong J. · 2011
Adult male Kunming mice were exposed to 900 MHz radiofrequency fields at power intensities of 12, 120 and 1200 μW/cm(2) for 1 h/day for 14 days and then subjected to whole body gamma-irradiation. Compared with mice exposed to gamma-radiation alone, the RF pre-exposed mice showed a significant increase in survival time and a reduction in hematopoietic tissue damage. The researchers described this effect as appearing similar to the 'adaptive response' which is well documented in scientific literature.
Aydin B, Akar A. · 2011
Researchers exposed immature and mature rats to a 900-MHz electromagnetic field for 2 hours per day for 45 days and measured oxidative stress in lymphoid organs, polymorphonuclear leukocytes, and plasma. Antioxidant enzyme activity, glutathione levels in lymphoid organs, and the antioxidant capacity of plasma decreased after exposure, while lipid peroxidation, nitric oxide levels, and myeloperoxidase activity increased. Improvements seen after a recovery period were limited, especially in immature rats, and oxidative damage was described as tissue specific.
Savić T, Janać B, Todorović D, Prolić Z. · 2011
Researchers investigated how a static magnetic field affected development and viability in Drosophila melanogaster and Drosophila hydei. Eggs of both species were exposed to an average magnetic induction of 60 mT throughout full development from egg to adult. Exposure reduced development time in both species, though statistical significance was found only for D. hydei, and viability was significantly weaker in both exposed species compared to controls.
Elferchichi M, Ammari M, Maaroufi K, Sakly M, Abdelmelek H. · 2011
Researchers exposed adult Wistar rats to a 128 millitesla static magnetic field for 1 hour per day for 5 consecutive days, then tested motor skills and measured iron levels in plasma and brain regions. No significant change in motor skills was observed between sham and exposed rats. Plasma transferrin content increased by 25.4% and plasma iron decreased by 16.2%, while brain iron concentration was not altered.
Amara S et al. · 2011
Researchers studied the effects of co-exposure to a static magnetic field (SMF) and cadmium on antioxidant enzyme activity and DNA integrity in rat brain. Cadmium exposure alone reduced antioxidant enzyme activity, including GPx, CAT, and SOD, in the frontal cortex and hippocampus, and increased markers of oxidative damage. When SMF (128 mT, 1 hour/day for 30 consecutive days) was combined with cadmium, SOD activity and glutathione levels in the frontal cortex fell further compared with the cadmium-only group, and MDA concentration also increased further. SMF exposure did not alter DNA oxidation levels in cadmium-exposed rats.
Monselise EB, Levkovitz A, Gottlieb HE, Kost D · 2011
Researchers exposed water plants (etiolated duckweed) for 24 hours to radio-frequency electromagnetic fields ranging between 1.8 V m(-1) and 7.8 V m(-1), generated by AM 1.287 MHz transmitting antennas. The exposure resulted in alanine accumulation in the plant cells, which the researchers describe as a universal stress signal. The magnitude of the effect corresponds qualitatively to the level of RF-EMF exposure. When vitamin C at 10 mM was present, alanine accumulation was completely suppressed, suggesting the involvement of free radicals in the process.
Kesari KK, Kumar S, Behari J. · 2011
The study investigated the effect of mobile phone exposure on fertility-related markers in 70-day-old male Wistar rats. Rats were exposed in Plexiglas cages for 2 hours a day for 35 days, with a specific absorption rate estimated at 0.9 W/kg. In exposed rats, antioxidant enzymes glutathione peroxidase and superoxide dismutase decreased, while malondialdehyde and free radical generation increased significantly. The researchers concluded that radiofrequency electromagnetic waves from commercially available cell phones might affect the fertilizing potential of spermatozoa.
Esmekaya MA, Ozer C, Seyhan N · 2011
Researchers exposed male Wistar albino rats to 900 MHz pulse-modulated RF radiation at a SAR of 1.20 W/kg for 20 minutes per day over three weeks to investigate oxidative damage in multiple tissues. MDA and NOx levels were significantly increased in liver, lung, testis, and heart tissues of the exposed group compared to sham and control groups, while GSH levels were significantly lower in exposed rat tissues. No significant difference was observed between the sham and control groups.
Chen YB, Li J, Liu JY, Zeng LH, Wan Y, Li YR, Ren D, Guo GZ. · 2011
Researchers exposed male BALB/c mice to electromagnetic pulses at a peak intensity of 400 kV/m for a total of 200 pulses, then measured associative learning, oxidative stress in the brain, and serum chemistry. Associative learning was reduced significantly at 12 hours and 1 day after exposure compared with sham control, but recovered at 2 days post exposure. Lipid peroxidation and chemiluminescence intensity in brain tissue increased significantly, while activity of antioxidant enzymes decreased significantly, at 3 h, 6 h, 12 h and 1 d post EMP exposure, with all parameters recovering at 2 days. Pretreatment with TMG showed protective effects against EMP exposure.
McCarty DE et al. · 2011
Zhao G et al. · 2011
The study examined cellular effects of a strong homogeneous static magnetic field in human-hamster hybrid (AL) cells, mitochondria-deficient cells, and double-strand break repair-deficient cells. ATP content was significantly decreased in AL cells exposed to 8.5 Tesla but not 1 or 4 T static magnetic field for either 3 or 5 hours. Reactive oxygen species levels were significantly increased by 8.5 T exposure, and concurrent treatment with the ROS inhibitor DMSO dramatically suppressed the ATP content in exposed AL cells. Mutation frequency and cell cycle distribution were not significantly affected by 8.5 T exposure for 3 hours.
Zeng L et al. · 2011
Researchers exposed male Sprague Dawley rats to electromagnetic pulse irradiation at 100 kV/m peak-to-peak electric field intensity to observe effects on the reproductive system. Sperm morphology, micronucleus formation rate, sperm number, and viability were not affected, but the acrosin reaction rate decreased at 24 h and 48 h and recovered by 72 h after irradiation. Enzymatic activities of T-SOD and MnSOD were reduced by 24 h, MDA content increased at 6 h, and serum LH and testosterone levels were elevated in irradiated rats compared to controls. The researchers suggested that the mechanism of EMP-induced damage may be associated with inhibition of MnSOD expression.
Sullivan K, Balin AK, Allen RG · 2011
Researchers determined the effects of a static magnetic field (SMF) on the proliferation of various types of human cells. SMF significantly decreased the initial attachment of fibroblasts and their subsequent growth, with effects observed in both fetal lung (WI-38) and adult skin fibroblast lines. SMF inhibited melanoma cell growth by 20% on day 7, but did not affect melanoma cell attachment. Oxidant production increased 37% in WI-38 cells exposed to SMF during the first 18 hours after seeding, but no elevation in oxidant levels was observed after a prolonged 5-day exposure.
Patruno A et al. · 2011
The study estimated kinetic constants of catalase, cytochrome P450, and inducible nitric oxide synthase in human HaCaT and THP-1 cell lines exposed to ELF-EMF. To evaluate cellular responses to an inflammatory stimulus, both cell lines were treated with lipopolysaccharide. The effects of ELF-EMF depend upon frequency, amplitude, and length of exposure, and are also related to intrinsic susceptibility and responsiveness of different cell types.
Osera C et al. · 2011
Researchers investigated the effects of an electromagnetic wave at 2 mT and 75 Hz on human neuroblastoma cells overexpressing amyloid precursor protein (APP). After prolonged electromagnetic treatment, lower mitochondrial activity and proliferation rate were observed, resulting in higher cellular quiescence. The treatment was also associated with an overall increase of HSP70 and superoxide dismutase-1 (SOD-1), and with increased expression of the enzyme ADAM10 and enhanced release of the neurotrophic factor sAPPα. The researchers suggested a possible use of this physical stimulation to improve the functional capability of cells to face noxae.
Martino CF, Castello PR · 2011
Researchers studied how suppressing the Earth's magnetic field to between 0.2 µT and 2 µT affected hydrogen peroxide production in several cell types. The cell types tested were a human fibrosarcoma cancer cell line, a pancreatic cancer cell line, and bovine pulmonary artery endothelial cells. Reducing the Earth's magnetic field suppressed hydrogen peroxide production in the cancer cells and the endothelial cells. Adding catalase and a superoxide dismutase mimetic called MnTBAP inhibited the magnetic field effect.
Martino CF · 2011
Researchers investigated how low level fields (0.2–1 µT), 30 µT, and 120 µT static magnetic fields affected the proliferation of human umbilical vein endothelial cells (HUVECs). Reducing the magnetic field to low levels clearly inhibited proliferation, while small differences of 30 µT reduced endothelial cell numbers significantly. Adding the free radical scavenger superoxide dismutase suppressed the enhanced proliferation caused by 120 µT static magnetic fields, suggesting a free radical mechanism of interaction.
Ghodbane S et al. · 2011
Researchers exposed male adult rats to a static magnetic field (128 mT, 1 hour per day for 5 days) and examined antioxidant enzymes and selenium levels in liver, kidney, muscle, and brain. Static magnetic field exposure decreased selenium levels in kidney, muscle, and brain, and decreased GPx activity in kidney and muscle. In the liver, static magnetic field exposure increased total GSH levels and total SOD activities, while glutathione reductase activity was unaffected. Selenium supplementation restored selenium levels and GPx activity in kidney and muscle, but did not bring down the elevated GSH and SOD levels in liver.
