Unknown authors · 2010
Researchers exposed H. pylori ATCC 43629 cultures and 2-day-old biofilm to ELF-EMF at 50 Hz and 1 mT for 2 days. Exposed cultures and sham-exposed controls were studied for cell viability, morphology, biofilm mass, genotypic profile, and luxS and amiA gene expression. During biofilm formation, ELF-EMF exposure significantly increased spiral forms to 58.41% versus 33.14% in controls and reduced biofilm cell mass. On mature biofilm, no significant differences were found compared to controls, though biofilm mass was also reduced. No changes in DNA patterns were recorded, but amiA gene expression was modulated.
Unknown authors · 2010
Unknown authors · 2010
Researchers induced diabetes in male Wistar rats with streptozocin and created a full-thickness dermal incision. The wound was exposed to ELF PEMF at 20 Hz, 4 ms, 8 mT for one hour per day. Healing rate was significantly greater in diabetic rats receiving PEMF compared to diabetic controls. Wound tensile strength was also significantly greater in PEMF-treated animals. Even non-diabetic control rats receiving PEMF healed faster than sham-exposed controls.
Unknown authors · 2010
Rats were exposed to 100 and 500 μT ELF-MF for 2 h/day during 10 months. Calcium levels decreased in the ELF-500 exposure group compared to the sham group. Magnesium levels decreased in the ELF-500 exposure group compared to both the sham and ELF-100 exposure groups. Zinc levels were lower in the ELF-500 exposure group than in the sham and ELF-100 exposure groups. No significant differences were found in phosphorus, copper, or iron levels between groups.
Unknown authors · 2010
Unknown authors · 2010
Researchers exposed human mesenchymal stem cells at passages 5 and 6 to homogeneous sinusoidal extremely low-frequency magnetic fields at 5 mT during chondrogenic differentiation. After 3 weeks, collagen type II expression increased significantly at passage 6 under EMF exposure. Aggrecan and SOX9 expression did not change significantly after EMF exposure. Collagen type X expression decreased under electromagnetic stimulation. Glycosaminoglycan content relative to DNA was higher in EMF-treated pellets at passage 5.
Unknown authors · 2010
The study used RAW 264.7 cells and RBL 2H3 cells. The cells were exposed to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours. Basal and melittin-induced arachidonic acid release, cPLA2, sPLA2, PLC, and PLD activities were unchanged by EMF exposure. The authors concluded that the exposure conditions, which were 2.4 fold higher than the occupational limit, did not induce phospholipase-associated signaling pathways.
Unknown authors · 2010
The group used early chick embryos and L929 and Daudi cells as experimental systems. The review found that ELF magnetic noise fields inhibited the effects when they were observed, and in all cases where a noise field was applied to prevent an observed effect it eliminated the effect.
Sekijima M et al. · 2010
The study examined cell proliferation and gene expression in three human cell lines after exposure to 2.1425 GHz continuous wave and W-CDMA modulated radiofrequency fields. Cells were exposed at specific absorption rates of 80, 250, or 800 mW/kg for up to 96 hours. No significant differences in cell growth or viability were found between exposed groups and sham controls, and less than 1% of genes showed altered expression.
Lee HJ et al. · 2010
Male rats were exposed to 848.5 MHz RF for 12 weeks. The exposure schedule consisted of two 45-min RF exposure periods separated by a 15-min interval. The whole-body average SAR was 2.0 W/kg. Researchers measured sperm counts, malondialdehyde concentrations, spermatogenesis stage frequency, germ cell counts, apoptotic cells, and performed immunoblotting for p53, bcl-2, caspase 3, p21, and PARP. The study concluded that subchronic exposure did not have observable adverse effects on rat spermatogenesis.
Kowalczuk C et al. · 2010
Researchers used a doubly resonant cavity to test whether biological samples generate a second harmonic when exposed to continuous wave RF fields at 883 MHz. They tested over 500 samples including human and mouse cell suspensions, adherent cell lines, and mouse tissue slices, both viable and non-viable. Input powers were 0.1 or 1 mW, and the system could detect second harmonic signals above a noise floor of -169 dBm. No consistent second harmonic was detected, so the results do not support the hypothesis that living cells can demodulate RF energy.
Kim KB et al. · 2010
Researchers exposed MCF7 human breast cancer cells to 849 MHz RF radiation for one hour per day over three consecutive days at SARs of 2 or 10 W/kg. Protein expression profiles were analyzed using two-dimensional gel electrophoresis and showed no significant or reproducible changes after exposure. Follow-up analysis of three non-reproducible protein spots using mass spectrometry, RT-PCR, and Western blot confirmed no alterations in mRNA or protein levels.
Hintzsche H, Stopper H. · 2010
Researchers collected buccal mucosa cells from 131 individuals and grouped them by mobile phone use. Cells were stained with alpha-tubulin-antibody and chromomycin A3, and micronuclei were counted in 2000 cells per person. Mobile phone use did not significantly increase micronucleus frequency.
Gurbuz N, Sirav B, Yuvaci HU, Turhan N, Coskun ZK, Seyhan N. · 2010
Researchers exposed female Wistar rats to 1800 MHz GSM-modulated RFR for 20 minutes per day, 5 days a week, for one month. They measured micronucleus frequency in exfoliated bladder cells to assess genotoxic damage. No significant difference in micronucleus frequency was found between exposed and control groups (p>0.05). The authors concluded that 1800 MHz RFR did not produce any genotoxic effect under these exposure conditions.
Falzone N, Huyser C, Franken DR, Leszczynski D. · 2010
Researchers examined effects of mobile phone radiation on apoptosis-related properties in human spermatozoa. Ejaculated, density-purified, highly motile human spermatozoa were exposed to mobile phone radiation at specific absorption rates of 2.0 and 5.7 W/kg. Mobile phone radiation had no statistically significant effect on any of the parameters studied, including caspase 3 activity, externalization of phosphatidylserine, DNA strand breaks, and reactive oxygen species generation. This suggests that impairment of fertility reported in some studies was not caused by induction of apoptosis in spermatozoa.
Bourthoumieu S et al. · 2010
Researchers exposed human amniotic cells to GSM-900 MHz radiofrequency radiation at an average SAR of 0.25 W/kg for 24 hours. Genotoxic effects were assessed immediately and 24 hours after exposure using R-banded karyotyping of 100 metaphase cells per assay across four samples. No direct cytogenetic effects were observed at either time point.
Markkanen A, Naarala J, Juutilainen J · 2010
Murine L929 fibroblast cells were exposed to 50 Hz magnetic fields of 100 or 300 μT during or before UV exposure. No significant magnetic field effects were found, and the results did not support the hypothesis that such fields modify biological responses to UV radiation by changing oxidative reactions.
O'Connor RP, Madison SD, Leveque P, Roderick HL, Bootman MD · 2010
Researchers used a high-throughput imaging platform to monitor calcium changes in cells exposed to 900 MHz GSM fields at specific absorption rates of 0.012–2 W/kg. The study examined resting calcium, agonist-evoked calcium signals, calcium store emptying, and store-operated calcium entry. GSM field data were compared with continuous wave fields and no-field controls. No changes in calcium signals were detected even at the highest field strengths, which exceeded typical phone exposure levels. The researchers concluded they could not detect any consequence of RF exposure under their experimental conditions.
Nylund R, Kuster N, Leszczynski D · 2010
Researchers exposed primary human umbilical vein endothelial cells and primary human brain microvascular endothelial cells to 1800 MHz GSM radiation for one hour at an average specific absorption rate of 2.0 W/kg. Protein expression patterns were examined immediately after exposure using two dimensional difference gel electrophoresis-based proteomics (2DE-DIGE). Exposure did not cause any statistically significant changes in protein expression in either cell type.
Finnie JW, Cai Z, Manavis J, Helps S, Blumbergs PC · 2010
Researchers exposed mice to 900 MHz RF fields at 4 W/kg for 60 minutes or five days per week for 104 weeks. Control mice were sham-exposed or freely moving in cages, and positive controls received a stab wound. No increase in microglial Iba1 expression was found in exposed brains compared to controls, while positive controls showed substantial activation. Acute or two-year exposure did not produce microglial activation detectable by Iba1 immunostaining.
Yu Y, Yao K. · 2010
It has been reported that exposure affects lens transparency, alters cell proliferation and apoptosis, inhibits gap junctional intercellular communication, and induces genetic instability and stress responses in lens epithelial cells. The authors note that high-power microwave radiation is well established to induce cataracts via thermal effects, but it remains unclear whether low-power microwave radiation below current exposure limits is cataractogenic. The review raises the question of whether ambient microwave environments can induce non-thermal effects in the lens with potential health consequences and calls for further in vivo studies.
Solomentsev GY, English NJ, Mooney DA · 2010
Meo SA, Al-Drees AM, Husain S, Khan MM, Imran MB · 2010
Researchers used 34 male Wistar albino rats divided into a control group and two groups exposed to mobile phone radiation for 30 or 60 minutes daily for three months. The 60-minute daily exposure group showed a significant decrease in serum testosterone compared to controls (p=0.028).
Goldwein O, Aframian DJ. · 2010
Fragopoulou AF, Koussoulakos SL, Margaritis LH. · 2010
Physically comparable pregnant mice were exposed to GSM 900 MHz radiofrequency radiation from a mobile phone. Within 5h after birth most pups were fixed and processed with double staining and paraffin histology, while other pups remained with their mothers until teeth eruption. Exposed newborns appeared normal externally, but histological examination revealed variations in cranial bone and rib ossification and displacement of Meckelian cartilage.
